Kultur in vitro pisang Kepok Merah (Musa paradisiaca) untuk mikropropagasi cepat

AbstractBanana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and are important in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L. For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA. NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan media Woody Plant (WP); ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/Ldan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0.5 mg/L yang dikombinasikan dengan BA 5 mg/L memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet. Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik

Download Full-text

Kultur in vitro pisang (Musa paradisiaca L.) cv. Kepok Merah untuk mikropropagasi cepat [In vitro culture of banana (Musa paradisiaca) cv. Kepok Merah for rapid micropropagation]

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v84i2.221 ◽

2017 ◽

Vol 84 (2) ◽

Author(s):

Efah FITRAMALA ◽

Eva KHAERUNNISA ◽

Nina Ratna Djuita Ratna DJUITA ◽

Hadi SUNARSO ◽

Diah RATNADEWI

Keyword(s):

Woody Plant ◽

Shoot Multiplication ◽

Ms Medium ◽

Musa Paradisiaca ◽

In Vitro Micropropagation ◽

The Media ◽

B Complex Vitamins ◽

Initiation Stage ◽

Planting Materials

Banana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) media were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L. For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA. NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. [Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.]AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan Woody Plant (WP), ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/L dan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0,5 mg/L yang dikombinasikan dengan BA 5 mg/L memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet. [Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik.]

Download Full-text

EMBRYO CULTURE AND IN VITRO CLONAL PROPAGATION OF OAK (Quercus aegilops L.)

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i1.934 ◽

2020 ◽

Vol 51 (1) ◽

Author(s):

Fadladeen & Toma

Keyword(s):

Embryo Culture ◽

Germination Rate ◽

Shoot Multiplication ◽

Ms Medium ◽

Suitable Alternative ◽

Culture Techniques ◽

Initiation Stage ◽

Propagation Methods ◽

Better Than

An attempt was done to develop a micropropagation protocol for oak using embryo culture. Oak is considered a hard-to-root woody plant by conventional propagation methods, that’s why using tissue culture techniques is a very suitable alternative method. For oak embryo culture, WPM was used and found to be better than MS medium for embryo germination which gave 66.13%. As well as adding of GA3 to the medium improved the germination rate of embryos (43.25% and 82.25 %). At initiation stage, WPM was used and found to be the best medium by giving the highest number of shoots/ explant which was 1.80, the highest number of leaves (15.17 leaves/ explant) and the longest shoots (1.42 cm) followed by MS medium then GD which gave the lowest parameters which gave 0.98 shoots/ explant, 7.20 leaves/ explant and 1.06 cm shoot length. At shoot multiplication stage, BA was better than Kinetin for multiplication of oak explants. The addition of BA at 3 mg.l-l gave the highest number of shoot and leaves which were 3.33 and 26.11 respectively. The longest shoots were achieved when 4.5 mg.l-l of BA was used. Furthermore, kinetin at 3 and 4.5 mg-l gave the lowest parameters which were 1 cm in length and 1.54 leaves/ explant. For rooting stage, NAA was better than IAA in giving better parameters and rooting percentage. The highest number of roots and rooting percentage were achieved when 1 mg.l-l was added by giving 6 roots/ explant and 100% rooting percentage. While the longest roots were achieved when 0.5 mg.l-l of NAA was used (3.67 cm) followed by 1.5 mg.l-l IAA which gave 3.55 roots/ explant with rooting percentage 90%. The produced plantlets were successfully acclimatized and transferred to the open-air conditions with a rate reached 85%.

Download Full-text

A Practical and Efficient Method for the Micropropagation of Japanese Cherry (Prunus sp.)

Vietnam Journal of Agricultural Sciences ◽

10.31817/vjas.2018.1.4.02 ◽

2019 ◽

Vol 1 (4) ◽

pp. 261-269

Author(s):

Thuy Linh Thi Nguyen ◽

Ngoc Thi Pham ◽

Thao Thi Ninh ◽

Phuong Thao Thi Nguyen

Keyword(s):

Climate Adaptation ◽

Shoot Multiplication ◽

Good Growth ◽

Multiplication Rate ◽

Ms Medium ◽

Rice Husks ◽

Shoot Initiation ◽

Primary Explants ◽

Planting Materials

This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA. The highest shoot multiplication rate (9.57 times) was obtained on MS medium containing 1 mg L-1 BA and 0.25 mg L-1 a-NAA after 8 weeks of culture. 100% of the shoots produced roots with a mean of 10.10 roots per plant within 4 weeks on ½ MSM medium with 4 mg L-1 IBA. The survival rate of in vitro derived plantlets after a 6 to 7-week-period of rooting during acclimatization using a soil: coco peat: smoked rice husks (2:2:1, v/v/v) substrate was 100% and acclimatized plantlets showed good growth and development. This is the first report on a practical and efficient in vitro multiplication protocol for Japanese cherry in Vietnam, starting from shoot initiation to establishment of plants under greenhouse conditions.

Download Full-text

APLIKASI SITOKININ TIPE PURIN DAN UREA PADA MULTIPLIKASI TUNAS ANIS (Pimpinellla anisum L.) IN VITRO

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v13n1.2007.1-7 ◽

2020 ◽

Vol 13 (1) ◽

pp. 1

Author(s):

OTIH ROSTIANA

Keyword(s):

Essential Oils ◽

Culture Media ◽

Shoot Multiplication ◽

Tropical Region ◽

Herbaceous Plant ◽

Solid Culture ◽

Pimpinella Anisum ◽

The Media ◽

Initiation Stage

ABSTRAK Anis (Pimpinella anisum L.) merupakan tanaman herba tahunan yang termasuk ke dalam famili Umbelliferae. Buahnya diketahui mengandung minyak atsiri yang didominasi senyawa trans-anethol (90%) dan berkhasiat sebagai antiseptik, antispasmodik, antikanker, karminatif, pelega tenggorokan, obat bronkitis, serta digunakan dalam pembuatan sabun, parfum, pasta gigi, juga krim kulit. Sebagai tanaman bernilai ekonomi, upaya perbanyakan anis perlu dilakukan. Perbanyakan secara in vitro dengan teknik kultur jaringan merupakan salah satu metode alternatif yang dapat digunakan untuk menghasilkan bibit dalam jumlah banyak, seragam dan dalam waktu yang relatif singkat. Dengan penambahan sitokinin sintetik tipe urea seperti thidiazuron (TDZ) dan tipe purin seperti benzil amino purin (BAP) akan memacu inisiasi dan proliferasi tunas. Penelitian ini bertujuan mendapatkan media yang tepat untuk menginduksi tunas anis yang optimal dengan penambahan BAP atau TDZ, mengetahui respon pertumbuhan dan penampakan kultur akibat penambahan berbagai konsentrasi BAP atau TDZ, serta mempelajari sinergisme yang terjadi antara keduanya. Pada tahap inisiasi, eksplan berupa tunas pucuk diinduksi di dalam media MS padat dengan penambahan BAP (0,1 mg/l; 0,2 mg/l; 0,3 mg/l; 1 mg/l; 2 mg/l; 3 mg/l), atau TDZ dengan kisaran konsentrasi yang sama. Tunas terbanyak yang dihasilkan dari dua jenis sitokinin pada tahap ini disubkultur ke dalam media yang ditambahkan jenis sitokinin yang berbeda (TDZ ke BAP atau BAP ke TDZ) pada konsentrasi 0,3 mg/l atau 3 mg/l. Pada media yang ditambahkan TDZ dihasilkan tunas anis lebih banyak (3,62-6,28) dibandingkan pada media yang ditambahkan BAP (1,86-2,78), tetapi tunas yang dihasilkan pendek (roset). Sedangkan tunas yang dihasilkan dalam media yang ditambahkan BAP beruas lebih tinggi tetapi jumlah tunasnya sedikit. Subkultur tunas anis ke dalam media yang diperkaya dengan sitokinin yang berbeda meningkatkan jumlah tunas yang berproliferasi dan memperbaiki visual tunas. Kata kunci: Anis, Pimpinellla anisum L. , minyak atsiri, multiplikasi tunas, in vitro, TDZ, BAP, Jawa Barat ABSTRACT Application of purine and urea types of cytokinins in shoot multiplication of Anise (Pimpinella anisum L.) in vitro Pimpinella anisum L. or sweet anise is an annual–herbaceous plant belongs to the Umbelliferae family. The fruit of anise contains of essential oil, which is mainly consisted of trans-anethol (90%). Essential oils of anise is mainly used as an antiseptic, antispasmodic, anticancer, carminative, expectorant and has also been used as component in soap, perfumery, tooth paste, and skin cream productions. Since this crop is mainly cultivated in sub tropical region, anise cultivation in Indonesia has not been performed. To obtain sufficient numbers of anise planting materials in vitro propagation was conducted by applying benzyl amino purine (BAP) and thidiazuron (TDZ). In this research TDZ or BAP were applied at various concentrations (0,1 mg/l: 0.2 mg/l; 0.3 mg/l; 1 mg/l; 2 mg/l; 3 mg/l), to induce shoots in MS-solid culture media. The highest number of shoots obtained in those two type of cytokinins containing media from the initiation stage were subcultured into the media supplemented with different cytokinins (TDZ to BAP or BAP to TDZ) at 0.3 mg/l or 3 mg/l levels. The results showed that medium with the addition of TDZ resulted in higher numbers of shoot (3,26-6,28) than that of medium with an addition of BAP (1,86-2,78). However, rosette shoots were dominant in TDZ containing medium. On the other hand, medium with an addition of BAP resulted in less numbers of shoots with taller nodes. Subculture of anise into different kinds of cytokinins increased the numbers of proliferated-shoots and recovered the abnormal shoots. Key words : Anise, Pimpinellla anisum L, essential oils, shoots multiplication, in vitro, TDZ, BAP, West Java

Download Full-text

TEKNIK BUDIDAYA IN VITRO Eleutherine sp. (Bawang Sabrang)

Jurnal Teknologi Lingkungan ◽

10.29122/jtl.v11i3.1179 ◽

2016 ◽

Vol 11 (3) ◽

pp. 341

Author(s):

Djadja Siti Hazar Hoesen

Keyword(s):

Acetic Acid ◽

Plant Growth Regulator ◽

Ms Medium ◽

Benzyl Adenine ◽

Soil Medium ◽

Shoot Buds ◽

The Media ◽

Initiation Stage ◽

Dichlorophenoxy Acetic Acid

Vegetative propagation from bulb excised of Eleutherine sp. (Iridaceae) were cultured in Murashige and Skoog (MS) medium supplemented with plant growth regulator (PGR) Benzyl adenine (BA) 1 mg/l at initiation stage, BA (2 and 5) mg/l for induced shoot buds formation at multiplication stage. In this study also BA 2 mg/l and BA 2 mg/l combined with naphthalein acetic acid (NAA) 0.5 mg/l were treated for rooting planlets formation. Calli formation were induced with auxin PGR picloram 1 mg/l combined with 2,4 dichlorophenoxy acetic acid (2,4-D) 0.5 and 1 mg/l in concentrations. The media contained 2 mg/l cytokinin (BA) without auxin (NAA), resulted the highest shoot buds formation. Rooting planlets were produced in MS medium combined with BA and NAA.MS medium contains Picloram 1 mg/l and 2,4-D 1 mg/l was optimal for frequency oncalli initiation (100%) and the largest diameter of calli also represented in cultured MS medium with picloram 1 mg/l and 2,4-D 1mg/l. In acclimatization stage, 100% of planlets survived and successfully transplanted to soil medium in the field.Key words: in vitro, Eleutherine sp.

Download Full-text

Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽

10.3390/agronomy11020320 ◽

2021 ◽

Vol 11 (2) ◽

pp. 320

Author(s):

Nisar Ahmad Zahid ◽

Hawa Z.E. Jaafar ◽

Mansor Hakiman

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Zingiber Officinale ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Zingiber Officinale Roscoe ◽

Planting Materials ◽

Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

Download Full-text

Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽

10.3390/molecules26113229 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3229

Author(s):

Mat Yunus Najhah ◽

Hawa Z. E. Jaafar ◽

Jaafar Juju Nakasha ◽

Mansor Hakiman

Keyword(s):

Callus Induction ◽

Antioxidant Activities ◽

Shoot Multiplication ◽

Wild Plants ◽

Wild Plant ◽

Total Phenolic ◽

Nodal Segment ◽

Ms Medium ◽

In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

Download Full-text

Shoot multiplication of Pogostemon cablin var. Sidikalang and patchouli oil profile

Nusantara Bioscience ◽

10.13057/nusbiosci/n110202 ◽

2019 ◽

Vol 11 (2) ◽

Author(s):

POPY HARTATIE HARDJO ◽

DANNY PUTRA SENTOSA SUSANTO ◽

WINA DIAN SAVITRI ◽

MARIA GORETTI MARIANTI PURWANTO

Keyword(s):

Growth Index ◽

Shoot Multiplication ◽

High Price ◽

Ms Medium ◽

Ex Vitro ◽

Average Growth ◽

In Vitro Multiplication ◽

Patchouli Alcohol ◽

Pogostemon Cablin

Abstract. Hardjo PH, Susanto DPS, Savitri WD, Purwanto MGM. 2019. Shoot multiplication of Pogostemon cablin var. Sidikalang and patchouli oil profile. Nusantara Bioscience 11: 123-127. Pogostemon cablin Benth. is a plant producing patchouli oil, which mostly consists of patchouli alcohol compound. Patchouli oil has great potentials in the world market because of its stability and high price. In this study, in vitro multiplication of Sidikalang variety of Acehnese patchouli shoots was done on solid and liquid Murashige & Skoog (MS) medium. This study aimed to determine the effect of cytokinins in various combinations of shoot multiplication and to compare the patchouli oil yield of in vitro and ex vitro culture. In vitro multiplication of Acehnese patchouli shoots by using solid MS medium with addition of 0.2 ppm benzyl aminopurine (BAP) and 0.2 ppm Kinetin resulted in shoot explants with an average growth index of 82.198 ± 0.690. Patchouli oil extraction was done on 7 weeks old in vitro shoot explants cultured on solid MS medium + 0.2 ppm BAP + 0.2 ppm Kinetin using water distillation method. In vitro shoots yielded 2.5% patchouli oil and contained ± 35% patchouli alcohol compound, whereas ex vitro shoots produced 4% patchouli oil and contained ± 25% patchouli alcohol compound. The qualitative analysis by using thin layer chromatography (TLC) showed that there were similarities in the number of spot and Rf value for each spot of ex vitro and in vitro patchouli oil.

Download Full-text

MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

CBU International Conference Proceedings ◽

10.12955/cbup.v6.1313 ◽

2018 ◽

Vol 6 ◽

pp. 1185-1191

Author(s):

Minh Van Tran

Keyword(s):

Somatic Embryogenesis ◽

Somatic Cell ◽

Embryonic Cell ◽

Cell Suspensions ◽

Ms Medium ◽

Callus Initiation ◽

Set Up ◽

Planting Materials ◽

In Vitro Embryogenesis

Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

Download Full-text

Research on in vitro micropropagation of Lilium brownii F.E. Brown

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/14/1/9302 ◽

2016 ◽

Vol 14 (1) ◽

pp. 121-129

Author(s):

Vũ Hoài Sâm ◽

Bùi Đức Quỳnh ◽

Nguyễn Thị Hương ◽

Nguyễn Văn Khiêm

Keyword(s):

Subtropical Climate ◽

Ms Medium ◽

In Vitro Plant Regeneration ◽

Medicinal Species ◽

Surface Sterilization ◽

The North ◽

In Vitro Micropropagation ◽

Half Strength ◽

Over Exploitation

Lilium brownii Brown belonging Lilium genus and Liliaceae family is well-known as a popular medicinal species, as well as food source and beautiful ornamental flowers. The specie has unique and ornamental floral characteristics such as light and elegant fragrance and perianth color rapidly changing from yellowish cream to white during anthesis. In traditional medicine, it is used for treatment cough, sedation diuretic, bronchitis... In nature, it can be found in subtropical climate moutainous areas in the North such as Sa Pa, Bat Xat, Mu Cang Chai; Sin Ho and Phong Tho, Quang Ba and Dong Van. In recent years, this species has been listed in the Red List for medicinal plants in Vietnam due to over-exploitation. The only effective strategy for sustaible conservation this species is in vitro micropropagation. In this study, in vitro plant regeneration and micropropagation of L. brownii was established from bubles and stem nodes. After surface sterilization with 0.1% HgCl2 in 10 minutes, healthy young shoots were obtained from initial bubles and stem nodes on MS medium supplemented with 0.5 mg/l BAP or 0.5 mg/l NAA, respectively. Bulblets also were formed from young shoot on MS supplemented with 0.5 mg/l NAA. The highest number of 4.5 bulblets per an explant was recorded from longitude-divided bubbles on MS medium containing 0.5 mg/l NAA and 0.2 mg/l BAP after 60 days in culture. The regererated plants produced quality roots on half strength MS supplemented with the combination of 1.0 mg/ l NAA and 0.2 mg / l BAP. More than 90% of rooted plants in vitro were survival on artificial soil TN1 in the nursery.

Download Full-text