scholarly journals Peningkatan kualitas planlet tanaman pisang raja bulu (Musa paradisiaca) dengan penambahan bap dan iaa pada media pengakaran kultur in vitro

2019 ◽  
Vol 3 (3) ◽  
pp. 132
Author(s):  
Rizal Try Nofiyanto ◽  
Florentina Kusmiyati ◽  
Karno Karno
Keyword(s):  
Musa Paradisiaca

Penelitian ini bertujuan untuk mengkaji pengaruh penambahan BAP dan IAA pada media pengakaran kultur jaringan terhadap pertumbuhan tanaman pisang raja bulu (Musa paradisiaca). Penelitian dilaksanakan di Laboratorium Kultur Jaringan Kebun Benih Hortikultura Salaman, Magelang pada bulan Juni hingga Agustus 2018. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) faktorial dengan 4 (empat) kali ulangan. Faktor pertama adalah hormon BAP dengan konsentrasi masing-masing 0 ; 0,5 ; 1 ; 1,5 ; dan 2 ppm, faktor kedua yaitu hormon IAA dengan konsentrasi 0, 1, 2, 3, dan 4 ppm. Kombinasi perlakuan sebanyak 25 dengan 4 kali ulangan, sehingga terdapat 100 unit percobaan yang setiap unit percobaan terdiri dari 5 planlet pisang raja bulu. Hasil penelitian menunjukkan bahwa terdapat pengaruh interaksi perlakuan BAP dan IAA terhadap parameter pertumbuhan planlet pisang raja bulu. Kombinasi BAP 1 ppm + IAA 3 ppm memberikan pengaruh terbaik pada jumlah daun dan diameter batang, sedangkan kombinasi BAP 0,5 ppm + IAA 4 ppm memberikan pengaruh terbaik pada jumlah akar dan panjang akar. Kombinasi BAP 1,5 ppm + IAA 4 ppm memberikan pengaruh terbaik pada tinggi planlet. Kesimpulan dari penelitian ini adalah zat pengatur tumbuh BAP 0,5 – 1,5 ppm + IAA 3 – 4 ppm direkomendasikan untuk media pengakaran kultur jaringan pisang raja bulu. Kata kunci: in vitro, BAP, IAA, plantlet, pisang

scholarly journals High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

2015 ◽  
Vol 3 (2) ◽  
pp. 202-209 ◽  
Author(s):  
R. Shashi Kumar ◽  
V. Krishna ◽  
. Venkatesh
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Synergetic Effect ◽  
Multiple Shoot ◽  
Coconut Water ◽  
Morphological Variations ◽  
Musa Paradisiaca ◽  
Shoot Initiation

High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 %) was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l), TDZ (0.1 to 1.2 mg/l) and coconut water (0.1 to 0.9 ml/l) induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg), number of hands in a bunch, number of fingers in a hand, length of the finger (cm), girth of the finger (cm) and girth of the pseudostem (cm) exhibited by in vitro plants were higher than the in vivo plants.Int J Appl Sci Biotechnol, Vol 3(2): 202-209 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12536 

scholarly journals Physical and chemical characteristics of enzymatically modified Batu banana (Musa balbisiana Colla) and Kepok banana (Musa paradisiaca formatypica) flours

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 124-131
Author(s):  
D.N. Afifah ◽  
Stephanie ◽  
A. Aulia ◽  
A. Rahadiyanti ◽  
D.M. Kurniawati ◽  
...  
Keyword(s):  
Water Absorption ◽  
Resistant Starch ◽  
Physical Characteristics ◽  
In Vitro Digestibility ◽  
Chemical Characteristics ◽  
Musa Balbisiana ◽  
Musa Paradisiaca ◽  
Banana Flour ◽  
Granular Morphology

Batu (Musa balbisiana Colla) and Kepok (Musa paradisiaca formatypica) bananas are two food commodities with high resistant starch content. The later has been suggested to prevent colorectal cancer. Modifications of these, into flour via the addition of pullulanase enzymes and autoclaving-cooling method are expected to improve the quality. The physical characteristics, levels of resistant starch, amylose, amylopectin and in vitro digestibility of enzymatically modified banana starches were determined. Therefore, a descriptive study was performed with untreated Batu banana flour (TP), autoclavingcooling (AC), autoclaving-cooling + pullulanase enzyme (AC + E), and autoclavingcooling + pullulanase enzyme + autoclaving-cooling (AC + E + AC). The granular morphology, water absorption, and swelling ability were evaluated as physical characteristics. Amylose, amylopectin content, and starch digestibility were evaluated as chemical characteristics. Based on SEM, the analysis of granular morphology showed structural modification in both treatments, although Batu banana flour showed greater water absorption and swelling ability, with and without AC + E + AC. However, both samples demonstrated better outcomes, in terms of high levels of starch resistance, low amylose, high amylopectin, and low in vitro digestibility after treatment with AC + E + AC.

scholarly journals Kultur in vitro pisang Kepok Merah (Musa paradisiaca) untuk mikropropagasi cepat

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Efah FITRAMALA ◽  
Eva KHAERUNNISA ◽  
Nina Ratna Djuita Ratna DJUITA ◽  
Hadi SUNARSO ◽  
Diah RATNADEWI
Keyword(s):  
Woody Plant ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Musa Paradisiaca ◽  
In Vitro Micropropagation ◽  
The Media ◽  
B Complex Vitamins ◽  
Initiation Stage ◽  
Planting Materials

AbstractBanana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and are important in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L.  For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA.  NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan media Woody Plant (WP); ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/Ldan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L   sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0.5 mg/L   yang dikombinasikan dengan BA 5 mg/L   memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L   pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet.  Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik

scholarly journals In vitro antibacterial, antioxidant, and cytotoxicity evaluations of Musa paradisiaca cv. Sekaki florets from Sarawak, Malaysia

2021 ◽  
Author(s):  
Ariffin Muhammad Mirza ◽  
Khong Heng Yen ◽  
Nyokat Nyotia ◽  
Liew Gee Moi ◽  
Hamzah Ahmad Sazali ◽  
...  
Keyword(s):  
Musa Paradisiaca

scholarly journals Fermentación ruminal y producción de metano usando la técnica de gas in vitro en forrajes de un sistema silvopastoril de ovinos de Chiapas, México

2019 ◽  
Vol 10 (2) ◽  
pp. 298-314
Author(s):  
Angel Jiménez Santiago ◽  
Guillermo Jiménez Ferrer ◽  
Armando Alayón-Gamboa ◽  
Esaú De Jesús Pérez-Luna ◽  
Angel Trinidad Piñeiro-Vázquez ◽  
...  
Keyword(s):  
Zea Mays ◽  
Leucaena Leucocephala ◽  
Zea Mays L ◽  
Gliricidia Sepium ◽  
Panicum Maximum ◽  
Musa Paradisiaca

Se evaluaron mediante la técnica de producción de gas in vitro, fuentes energéticas locales (melaza, Zea mays L. y Musa paradisiaca L.) sobre la fermentación ruminal y producción de metano de diversos forrajes usados en un sistema silvopastoril con Panicum maximum cv. Tanzania, Gliricidia sepium (Jacq.) y Leucaena leucocephala cv. Cunningham, con ovinos. Se usaron cinco borregos Pelibuey x Katahdin 40 ± 3 (μ±DE) kg como donantes de líquido ruminal. Se analizaron cinco tratamientos (dietas) con diferentes mezclas de follaje de arbóreas y fuentes energéticas en un diseño experimental completamente al azar. M. paradisiaca y Z. mays presentaron los mayores registros de volumen (V) máximo en producción de gas (544 y 467 ml/g-1 MS, respectivamente) (P≤0.05). El follaje de G. sepium y L. leucocephala tuvieron los menores valores de V (253 y 180 ml/g-1 MS, respectivamente) (P≤0.05). La dieta D4 GMP (48 % P. maximum, 30 % G. sepium, 7 % Zea mays, 15 % M. paradisiaca) registro el mayor valor de V. No hubo diferencia (P>0.05) en la producción de metano en las dietas usadas, teniendo un rango de 6.31 a 9.60 de LCH4/kg MSDIG. Se generó un índice de emisión potencial de gases fermentables (IPEGF), el cual sugirió que dietas con carbohidratos de lenta fermentación, contribuyen a un índice más alto de emisión de gases. Por su mejoramiento en la calidad de las dietas y en contribuir en una baja de emisiones de CH4, se sugiere el manejo de arbóreas forrajeras como G. sepium y L. leucocephala, incorporando fuentes energéticas locales.

scholarly journals Índice Glucémico in vitro, Contenido fenólico y Actividad Antioxidante de Snacks Elaborados con Harinas de Plátano (Musa paradisiaca) y Yacón (Smallanthus sonchifolius)

2019 ◽  
Vol 30 (5) ◽  
pp. 111-120 ◽  
Author(s):  
Alejandra Díaz-Osorio ◽  
Marcela Martínez-Castaño ◽  
José Contreras-Calderon ◽  
Cecilia Gallardo-Cabrera
Keyword(s):  
Smallanthus Sonchifolius ◽  
Musa Paradisiaca

scholarly journals Anticancer potential of musa × paradisiaca as cervical carcinoma and malignant melanoma

2020 ◽  
Author(s):  
Aakash Deep ◽  
Om Narayan Upadhyay ◽  
Rimmy Nandal ◽  
Sanjiv Kumar ◽  
Arun Kumar Sharma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Malignant Melanoma ◽  
Ethyl Acetate ◽  
Cervical Carcinoma ◽  
Ethyl Acetate Extract ◽  
Significant Inhibition ◽  
Gas Chromatography Mass Spectrometry ◽  
Musa Paradisiaca ◽  
And Oxidative Stress

Abstract This study was performed to assess the anticancer potential of Musa × pardisiaca L. It has several traditional benefits due to the presence of various bioactive compounds. Ethyl acetate extract of leaves was prepared and characterized by Gas Chromatography-Mass spectrometry (GC-MS). Compounds ( 1-40 ) were isolated from leaves, out of which two major compounds i.e. eugenol and phytol are potentially responsible for anticancer activity and oxidative stress. In vitro cytotoxicity of extract showed significant inhibition of cells in cervical carcinoma and malignant melanoma when treated by MTT assay against Hela and A375 cell lines. Ethyl acetate extract of musa × paradisiaca L. shown anticervical carcinoma and anti-malignant melanoma activity in our study.

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