Fungistatic Activity Of Essential Oils Extracted from Peumus boldus Mol., Laureliopsis philippiana (Looser) Schodde and Laurelia sempervirens (Ruiz & Pav.) Tul. (Chilean Monimiaceae)

Candidiasis therapy, especially for candidiasis caused by Candida non-albicans species, is limited by the relatively reduced number of antifungal drugs and the emergence of antifungal tolerance. This study evaluates the anticandidal activity of 41 plant-derived products against Candida species, in both planktonic and biofilm cells. This study also evaluates the toxicity and the therapeutic action of the most active compounds by using the Caenorhabditis elegans–Candida model. The planktonic cells were cultured with various concentrations of the tested agents. The Cupressus sempervirens, Citrus limon, and Litsea cubeba essential oils as well as gallic acid were the most active anticandidal compounds. Candida cell re-growth after treatment with these agents for 48 h demonstrated that the L. cubeba essential oil and gallic acid displayed fungistatic activity, whereas the C. limon and C. sempervirens essential oils exhibited fungicidal activity. The C. sempervirens essential oil was not toxic and increased the survival of C. elegans worms infected with C. glabrata or C. orthopsilosis. All the plant-derived products assayed at 250 µg/mL affected C. krusei biofilms. The tested plant-derived products proved to be potential therapeutic agents against Candida, especially Candida non-albicans species, and should be considered when developing new anticandidal agents.

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Fungistatic activity of essential oils for the control of bipolaris leaf spot in maize

Journal of Medicinal Plants Research ◽

10.5897/jmpr2019.6781 ◽

2019 ◽

Vol 13 (12) ◽

pp. 280-287

Author(s):

De Souza Carlos Mourao Dalmarcia ◽

Rodrigues De Souza Micaele ◽

Vinícius Lopes Dos Reis Joao ◽

Pereira De Souza Ferreira Talita ◽

Raymundo Argüelles Osorio Pedro ◽

...

Keyword(s):

Essential Oils ◽

Leaf Spot ◽

Fungistatic Activity

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Fungistatic Activity of Essential Oils from Ocimum basilicum Chemotypes

Journal of Phytopathology ◽

10.1111/j.1439-0434.1984.tb00736.x ◽

1984 ◽

Vol 110 (1) ◽

pp. 20-22 ◽

Cited By ~ 46

Author(s):

R. Reuveni, A. Fleischer ◽

E. Putievsky

Keyword(s):

Essential Oils ◽

Ocimum Basilicum ◽

Fungistatic Activity

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Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous Fusarium Isolates Obtained from Cereal Grains in Southern Poland

Molecules ◽

10.3390/molecules25020292 ◽

2020 ◽

Vol 25 (2) ◽

pp. 292 ◽

Cited By ~ 2

Author(s):

Teresa Krzyśko-Łupicka ◽

Sławomir Sokół ◽

Anna Piekarska-Stachowiak

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Gas Chromatography Mass Spectrometry ◽

Ionization Time ◽

Tea Tree ◽

Fungistatic Activity ◽

Litsea Cubeba ◽

Synthetic Pesticide ◽

The Relationship

The aim of the study was to determine the relationship between the chemical composition of eight commercial essential oils (EsO) (garlic, grapefruit, lemon grass, tea tree, thyme, verbena, cajeput, and Litsea cubeba) and their fungistatic activity in relation to four species of Fusarium: F. avenaceum, F. culmorum, F. graminearum, and F. oxysporum. The species identification of Fusarium isolates was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The determination of qualitative and quantitative chemical composition of the EsO was carried out using the gas chromatography–mass spectrometry (GC–MS) method. The fungistatic activity of EsO was assessed by using the method of poisoned substrates. The data were compiled in the STATISTICA 13.0 program. The chemical composition of the tested oils varied; the dominant fraction, except for grapefruit and garlic oils, were monoterpenoids. The greatest similarity to the action of the synthetic pesticide Funaben T was found in four oils, i.e., thyme, lemongrass, verbena, and Litsea cubeba. The studies showed that F. oxysporum and F. avenaceum were characterized by a higher resistance to low oil concentrations, and F. culmorum and F. graminearum by sensitivity. The fungicidal activity of two EsO-dominant monoterpenoids-thymol and citral—has been confirmed.

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The Concentration-Dependent Effects of Essential Oils on the Growth of Fusarium graminearum and Mycotoxins Biosynthesis in Wheat and Maize Grain

Applied Sciences ◽

10.3390/app12010473 ◽

2022 ◽

Vol 12 (1) ◽

pp. 473

Author(s):

Daniela Gwiazdowska ◽

Katarzyna Marchwińska ◽

Krzysztof Juś ◽

Pascaline Aimee Uwineza ◽

Romuald Gwiazdowski ◽

...

Keyword(s):

Essential Oils ◽

Plant Protection ◽

Animal Health ◽

Protection Factor ◽

High Biological Activity ◽

Fungistatic Activity ◽

Complex Chemical Composition ◽

Maize Grain ◽

Mycotoxin Biosynthesis ◽

Fusarium Fungi

The presence of Fusarium fungi and their toxic metabolites in agricultural crops contributes to significant quantitative and qualitative losses of crops, causing a direct threat to human and animal health and life. Modern strategies for reducing the level of fungi and mycotoxins in the food chain tend to rely on natural methods, including plant substances. Essential oils (EOs), due to their complex chemical composition, show high biological activity, including fungistatic properties, which means that they exhibit high potential as a biological plant protection factor. The aim of this study was to determine the fungistatic activity of three EOs against F. graminearum, and the reduction of mycotoxin biosynthesis in corn and wheat grain. All tested EOs effectively suppressed the growth of F. graminearum in concentrations of 5% and 10%. Cinnamon and verbena EOs also effectively reduced the ergosterol (ERG) content in both grains at the concentration of 1%, while at the 0.1% EO concentration, the reduction in the ERG amount depended on the EO type as well as on the grain. The degree of zearalenone (ZEA) reduction was consistent with the inhibition of ERG biosynthesis, while the reduction in deoxynivalenol (DON) was not consistent with this parameter.

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Fungistatic activity of essential oils towards selected oilseed rape pathogens

Progress in Plant Protection ◽

10.14199/ppp-2018-042 ◽

2018 ◽

Cited By ~ 1

Keyword(s):

Essential Oils ◽

Oilseed Rape ◽

Fungistatic Activity

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FUNGITOXICITY OF ESSENTIAL OILS ON PLASMOPARA VITICOLA,CAUSAL AGENT OF GRAPEVINE DOWNY MILDEW

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452017015 ◽

2017 ◽

Vol 39 (4) ◽

Author(s):

RÉGIS DE OLIVEIRA FIALHO ◽

MARLI DE FÁTIMA STRADIOTO PAPA ◽

ALAN RODRIGO PANOSSO ◽

ANA MARIA RODRIGUES CASSIOLATO

Keyword(s):

Essential Oils ◽

Downy Mildew ◽

Eucalyptus Globulus ◽

Direct Contact ◽

Plasmopara Viticola ◽

Causal Agent ◽

Antifungal Effect ◽

Grapevine Downy Mildew ◽

The Third ◽

Fungistatic Activity

ABSTRACT This study aimed to evaluate the fungitoxic (antifungal) effect of seven essential oils on Plasmopara viticola, the causal agent of grapevine downy mildew. The cinnamon, eucalyptus globulus, marjoram, melaleuca, peppermint, oregano and white thyme essential oils were compared with the mancozeb + metalaxyl-M fungicide. Three experiments were conducted to evaluate the effect of essential oils on spore germination. The first experiment evaluated the effect of direct contact of essential oils on the pathogen; the second evaluated the effect of volatile compounds on the pathogen and the third assessed the behavior of essential oils, when applied to vine leaves in field conditions, on the pathogen. All essential oils inhibited the spores’ germination, but in varying degrees, in addition, presenting fungistatic activity. All the essential oils showed chemical instability when the incubation time or the interval between products application where increased, all oils decrease antifungal activity percentage. Noteworthy, the cinnamon and the eucalyptus globulus essential oils were the most fungitoxic (antifungal) on P. viticola.

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Effectiveness of the Influence of Selected Essential Oils on the Growth of Parasitic Fusarium Isolated from Wheat Kernels from Central Europe

Molecules ◽

10.3390/molecules26216488 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6488

Author(s):

Teresa Krzyśko-Łupicka ◽

Sławomir Sokół ◽

Monika Sporek ◽

Anna Piekarska-Stachowiak ◽

Weronika Walkowiak-Lubczyk ◽

...

Keyword(s):

Growth Rate ◽

Essential Oils ◽

Fungicidal Activity ◽

German Population ◽

Polish Population ◽

Fusarium Spp ◽

Second Stage ◽

Fungistatic Activity ◽

Litsea Cubeba ◽

Wheat Kernels

The aim of the study was to determine the effectiveness of selected seven commercial essential oils (EsO) (grapefruit, lemongrass, tea tree (TTO), thyme, verbena, cajeput, and Litsea cubeba) on isolates of common Central European parasitic fungal species of Fusarium obtained from infected wheat kernels, and to evaluate the oils as potential natural fungicides. The study was conducted in 2 stages. At each stage, the fungicidal activity of EsO (with concentrations of 0.025; 0.05; 0.125; 0.25; 0.50; 1.0, and 2.0%) against Fusarium spp. was evaluated using the disc plate method and zones of growth inhibition were measured. At the first stage, the fungistatic activity of EsO was evaluated against four species of Fusarium from the Polish population (F. avenaceum FAPL, F. culmorum FCPL, F. graminearum FGPL and F. oxysporum FOPL). The correlation coefficient between the mycelial growth rate index (T) and the fungistatic activity (FA) was calculated. At the second stage, on the basis of the mycelium growth rate index, the effectiveness of the EsO in limiting the development of Fusarium isolates from the German population (F. culmorum FC1D, F. culmorum FC2D, F. graminearum FG1D, F. graminearum FG2D and F. poae FP0D) was assessed. The first and second stage results presented as a growth rate index were then used to indicate essential oils (as potential natural fungicides) effectively limiting the development of various common Central European parasitic species Fusarium spp. Finally, the sensitivity of four Fusarium isolates from the Polish population and five Fusarium isolates from the German population was compared. The data were compiled in STATISTICA 13.0 (StatSoft, Inc, CA, USA) at the significance level of 0.05. Fusarium isolates from the German population were generally more sensitive than those from the Polish population. The sensitivity of individual Fusarium species varied. Their vulnerability, regardless of the isolate origin, in order from the most to the least sensitive, is as follows: F. culmorum, F. graminearum, F. poae, F. avenaceum and F. oxysporum. The strongest fungicidal activity, similar to Funaben T, showed thyme oil (regardless of the concentration). Performance of citral oils (lemongrass and Litsea cubeba) was similar but at a concentration above 0.025%.

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