scholarly journals Effectiveness of the Influence of Selected Essential Oils on the Growth of Parasitic Fusarium Isolated from Wheat Kernels from Central Europe

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6488
Author(s):  
Teresa Krzyśko-Łupicka ◽  
Sławomir Sokół ◽  
Monika Sporek ◽  
Anna Piekarska-Stachowiak ◽  
Weronika Walkowiak-Lubczyk ◽  
...  
Keyword(s):  
Growth Rate ◽  
Essential Oils ◽  
Fungicidal Activity ◽  
German Population ◽  
Polish Population ◽  
Fusarium Spp ◽  
Second Stage ◽  
Fungistatic Activity ◽  
Litsea Cubeba ◽  
Wheat Kernels

The aim of the study was to determine the effectiveness of selected seven commercial essential oils (EsO) (grapefruit, lemongrass, tea tree (TTO), thyme, verbena, cajeput, and Litsea cubeba) on isolates of common Central European parasitic fungal species of Fusarium obtained from infected wheat kernels, and to evaluate the oils as potential natural fungicides. The study was conducted in 2 stages. At each stage, the fungicidal activity of EsO (with concentrations of 0.025; 0.05; 0.125; 0.25; 0.50; 1.0, and 2.0%) against Fusarium spp. was evaluated using the disc plate method and zones of growth inhibition were measured. At the first stage, the fungistatic activity of EsO was evaluated against four species of Fusarium from the Polish population (F. avenaceum FAPL, F. culmorum FCPL, F. graminearum FGPL and F. oxysporum FOPL). The correlation coefficient between the mycelial growth rate index (T) and the fungistatic activity (FA) was calculated. At the second stage, on the basis of the mycelium growth rate index, the effectiveness of the EsO in limiting the development of Fusarium isolates from the German population (F. culmorum FC1D, F. culmorum FC2D, F. graminearum FG1D, F. graminearum FG2D and F. poae FP0D) was assessed. The first and second stage results presented as a growth rate index were then used to indicate essential oils (as potential natural fungicides) effectively limiting the development of various common Central European parasitic species Fusarium spp. Finally, the sensitivity of four Fusarium isolates from the Polish population and five Fusarium isolates from the German population was compared. The data were compiled in STATISTICA 13.0 (StatSoft, Inc, CA, USA) at the significance level of 0.05. Fusarium isolates from the German population were generally more sensitive than those from the Polish population. The sensitivity of individual Fusarium species varied. Their vulnerability, regardless of the isolate origin, in order from the most to the least sensitive, is as follows: F. culmorum, F. graminearum, F. poae, F. avenaceum and F. oxysporum. The strongest fungicidal activity, similar to Funaben T, showed thyme oil (regardless of the concentration). Performance of citral oils (lemongrass and Litsea cubeba) was similar but at a concentration above 0.025%.

scholarly journals Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous Fusarium Isolates Obtained from Cereal Grains in Southern Poland

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 292 ◽  
Author(s):  
Teresa Krzyśko-Łupicka ◽  
Sławomir Sokół ◽  
Anna Piekarska-Stachowiak
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Time ◽  
Tea Tree ◽  
Fungistatic Activity ◽  
Litsea Cubeba ◽  
Synthetic Pesticide ◽  
The Relationship

The aim of the study was to determine the relationship between the chemical composition of eight commercial essential oils (EsO) (garlic, grapefruit, lemon grass, tea tree, thyme, verbena, cajeput, and Litsea cubeba) and their fungistatic activity in relation to four species of Fusarium: F. avenaceum, F. culmorum, F. graminearum, and F. oxysporum. The species identification of Fusarium isolates was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The determination of qualitative and quantitative chemical composition of the EsO was carried out using the gas chromatography–mass spectrometry (GC–MS) method. The fungistatic activity of EsO was assessed by using the method of poisoned substrates. The data were compiled in the STATISTICA 13.0 program. The chemical composition of the tested oils varied; the dominant fraction, except for grapefruit and garlic oils, were monoterpenoids. The greatest similarity to the action of the synthetic pesticide Funaben T was found in four oils, i.e., thyme, lemongrass, verbena, and Litsea cubeba. The studies showed that F. oxysporum and F. avenaceum were characterized by a higher resistance to low oil concentrations, and F. culmorum and F. graminearum by sensitivity. The fungicidal activity of two EsO-dominant monoterpenoids-thymol and citral—has been confirmed.

scholarly journals Antifungal activities of thiosemicarbazones and semicarbazones against mycotoxigenic fungi

2014 ◽  
Vol 38 (6) ◽  
pp. 531-537 ◽  
Author(s):  
Rojane de Oliveira Paiva ◽  
Lucimar Ferreira Kneipp ◽  
Carla Marins Goular ◽  
Mariana Almeida Albuquerque ◽  
Aurea Echevarria
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Flavus ◽  
Fungicidal Activity ◽  
Fusarium Verticillioides ◽  
Animal Health ◽  
Fungistatic Activity ◽  
Mycotoxigenic Fungi ◽  
Quality Of Food ◽  
Chelating Effect

Mycotoxigenic fungi can compromise the quality of food, exposing human and animal health at risk. The antifungal activity of eight thiosemicarbazones (1-8) and nine semicarbazones (9-17) was evaluated against Aspergillus flavus, A. nomius, A. ochraceus, A. parasiticus and Fusarium verticillioides. Thiosemicarbazones had MIC values of 125-500 µg/ml. The thiosemicarbazones 1 and 2 exerted fungistatic activity against Aspergillus spp., and thiosemicarbazone 2 exerted fungicidal activity against F. verticillioides. Compound 2 showed an iron chelating effect of 63%. The ergosterol content of A. parasiticus had a decrease of 28 and 71% for the 31.2 and 62.5 µg/ml concentrations of thiosemicarbazone 2 compared to the control. The obtained results of antifungal activity revealed that thiosemicarbazone class was more active when compared to semicarbazone class and, the thiosemicarbazone 2 was the most active compound, specially, against Aspergillus spp.

Chemical composition, antimicrobial activity and antioxidant activity of Litsea cubeba essential oils in different months

2019 ◽  
Vol 34 (22) ◽  
pp. 3285-3288 ◽  
Author(s):  
Qi-Hui She ◽  
Wen-Shuang Li ◽  
Yuan-Yuan Jiang ◽  
Yi-Chao Wu ◽  
Yong-Hong Zhou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Litsea Cubeba

scholarly journals Chemical Composition and Antimicrobial Activity of Selected Essential Oils against Staphylococcus spp. Isolated from Human Semen

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 765
Author(s):  
Miroslava Kačániová ◽  
Margarita Terentjeva ◽  
Jana Štefániková ◽  
Jana Žiarovská ◽  
Tatsiana Savitskaya ◽  
...  
Keyword(s):  
Essential Oils ◽  
Chemical Properties ◽  
Citrus Limon ◽  
Santalum Album ◽  
Cinnamomum Camphora ◽  
Human Semen ◽  
Litsea Cubeba ◽  
Wide Range ◽  
Staphylococcus Spp ◽  
Santalum Album L

Staphylococcus spp. is not only a commensal bacteria but also a major human pathogen that causes a wide range of clinical infections. Recent evidence suggests that Staphylococcus has the ability to colonize the reproductive system and to affect its structure and functions. The objective of this study was to determine the chemical properties and antibacterial effects of select essential oils (EOs): Amyris balsamifera L., Boswellia carterii Birdw., Canarium luzonicum (Blume) A. Gray, Cinnamomum camphora (L.) J. Presl., Cinnamomum camphora var. linaloolifera Y. Fuita, Citrus x aurantium L., Gaultheria procumbens L., Litsea cubeba (Lour.) Pers., Melaleuca ericifolia Smith., Melaleuca leucadendra L., Pogostemon cablin (Blanco) Benth., Citrus limon (L.) Osbeck, Santalum album L., and Vetiveria zizanoides (L.) Roberty against 50 Staphylococcus spp. cultures isolated from human semen, specifically Staphylococcus aureus, S. capiti, S. epidermidis, S. haemoliticus, and S. hominis. The disc diffusion and broth microdilution methods were used to assess the antimicrobial potential and to determine the minimum inhibitory concentration (MIC) of the selected EOs. The best anti-Staphylococcus activities were found with both methods for the essential oils of C. luzonicum (Blume) A. Gray, A. balsamifera, C. camphora, and P. cabli.

scholarly journals Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia

2016 ◽  
Vol 44 (2) ◽  
pp. 411-417 ◽  
Author(s):  
Snežana PAVLOVIC ◽  
Danijela RISTIC ◽  
Ivan VUCUROVIC ◽  
Miloš STEVANOVIC ◽  
Saša STOJANOVIC ◽  
...  
Keyword(s):  
Essential Oils ◽  
Molecular Identification ◽  
Fusarium Species ◽  
Fusarium Spp ◽  
First Report ◽  
Pimpinella Anisum ◽  
Health Inspection ◽  
The Family ◽  
The World

Anise (Pimpinella anisum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mošorin, Veliki Radinci and Ostojićevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungion the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. tricinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sporotrichoides, F. incarnatum and F. verticillioides. According to our knowledge and research, this is the first report of F. tricinctum and F. sporotrichoides as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricinctum and F. incarnatum.

Fungicidal activity of cecropin A.

1997 ◽  
Vol 41 (2) ◽  
pp. 481-483 ◽  
Author(s):  
A J DeLucca ◽  
J M Bland ◽  
T J Jacks ◽  
C Grimm ◽  
T E Cleveland ◽  
...  
Keyword(s):  
Fungicidal Activity ◽  
Lethal Concentration ◽  
Fusarium Spp ◽  
Rpmi Medium ◽  
Cecropin A ◽  
Minimal Lethal Concentration

Cecropin A (CA) fungicidal properties were explored. Nongerminated and germinated Aspergillus spp. and Fusarium spp. conidia were treated with CA. CA achieved complete lethality at < or = 25 microM (99 micrograms/ml) for germinating, but not nongerminating, conidia of Aspergillus spp. CA achieved total lethality for nongerminated and germinating conidia of Fusarium spp at 1.5 microM (6 micrograms/ml). MIC and minimal lethal concentration assays in buffered RPMI medium gave similar results.

scholarly journals Assessment of Candida auris Response to Antifungal Drugs Using Time–Kill Assays and an Animal Model

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S73-S73 ◽  
Author(s):  
Ronen Ben-Ami ◽  
Liat Ashkenazi ◽  
Judith Berman ◽  
Nuphar Korolker ◽  
Anna Novikov
Keyword(s):  
Amphotericin B ◽  
Fungicidal Activity ◽  
Antifungal Drugs ◽  
Rank Test ◽  
Infection Model ◽  
Candida Auris ◽  
Fungistatic Activity ◽  
Kill Curve ◽  
Time Kill

Abstract Background Candida auris is an emerging nosocomial pathogen that is resistant to Fluconazole and variably susceptible to other systemic drug classes. Treatment with echinocandins has been recommended based on MICs in the susceptible range, but supporting in vivo data is lacking. Methods We tested the MIC of C. auris strains (n = 12) to fluconazole, voriconazole, posaconazole. anidulafungin, amphotericin B and flucytosine. Representative C. auris strains from Israel and South Africa, and a reference C. albicans strain were analysed using time–kill curve assays. Fungicidal activity was defined as reduction of ≥3 log from baseline CFU/ml. Response to caspofungin treatment was assessed in BALB/c mice immunosuppressed with cyclophosphamide and inoculated with 7 × 107C. auris cells by tail vein injection. Mice were treated from day +1 to day +7 with caspofungin (IP) at doses of 1 or 5 mg/kg and compared with sham-treated controls. Survival was assessed daily. Kaplan-Meier survival analyses were performed and treatment arms were compared using the log-rank test. Results Drug susceptibility results (MIC50 and MIC90) were: fluconazole, 64 and 128 mg/l; voriconazole, 0.5 and 24 mg/l; posaconazole, 0.5 and 27 mg/l; anidulafungin, 0.03 and 0.06 mg/l; amphotericin B, 2 and 8 mg/l; flucytosine, 0.3 and 1 mg/l. Time–kill curve analyses showed log reduction from baseline CFU concentration of −3.0 to −2.8 for fluconazole (MIC ×1), 5.6–6.1 for amphotericin B (MIC ×4) and −0.4 to −0.9 for caspofungin (MIC ×16), consistent with fungicidal activity of amphotericin B and weak fungistatic activity of caspofungin. In the mouse model, survival rate was similar with sham treatment (33%) and treatment with caspofungin 1 mg/kg/day (44%) and 5 mg/kg/day (22%), P = 0.7. Conclusion Despite generally low MIC, caspofungin has only mild fungistatic activity on C. auris and no effect on survival in a mouse infection model. Amphotericin B has fungicidal activity against C. auris. Disclosures All authors: No reported disclosures.

scholarly journals Weissella cibaria Fungistatic Activity against Fusarium spp. affecting Yellow Pitahaya

2016 ◽  
Vol 13 (12) ◽  
pp. 1354-1364 ◽  
Author(s):  
Leidy Johana Valencia-Hernández ◽  
Karina López-López ◽  
Liliana Serna-Cock
Keyword(s):  
Fusarium Spp ◽  
Weissella Cibaria ◽  
Fungistatic Activity
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