scholarly journals Antioxidant capacity, phenolic and vitamin C contents of quinoa (Chenopodium quinoa Willd.) as affected by sprouting and storage conditions

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Maura N. Laus ◽  
Mariagrazia P. Cataldi ◽  
Carlo Robbe ◽  
Tiziana D'Ambrosio ◽  
Maria L. Amodio ◽  
...  
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Peroxyl Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Chenopodium Quinoa ◽  
Storage Conditions ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Reducing Activity

Antioxidant capacity (AC) of quinoa (<em>Chenopodium quinoa</em> Willd. cv. Real) seeds and sprouts obtained after 4 days of seed germination at 20°C and 70% humidity was evaluated using trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays, able to highlight reducing activity and peroxyl radical scavenging capacity, respectively; phenolic content (PC) was also measured. Both TEAC and ORAC assays revealed a significantly higher (about 2- and 2.8-fold, respectively) AC of 4-day-old sprouts compared to seeds; consistently, also PC values of sprouts resulted about 2.6 times higher than seeds. In order to investigate the influence of storage on AC and PC, as well as on vitamin C content (VCC), 4-day-old sprouts were subjected for 7 days at 5°C to three different conditions of controlled atmosphere storage (CAS) compared with air. Interestingly, whatever the CAS conditions, storage of quinoa sprouts up to 7 days induced an increase of AC evaluated in terms of reducing activity by TEAC assay. Consistently, an increase of PC and VCC was measured during storage, positively correlated to TEAC values. Moreover, a decrease of peroxyl radical scavenging activity, measured by ORAC, was observed after 7 days of storage, in accordance with a shift of AC towards the reducing activity component. Overall, these findings indicate that sprouting approach using quinoa may provide highly antioxidant-enriched seedlings that may improve nutritional quality of diet or of functional foods. Interestingly, antioxidant properties of quinoa sprouts may be deeply influenced by storage, able to increase reducing activity by increasing phenols and vitamin C.

scholarly journals Determination of Flavonoid and Proanthocyanidin Profile of Hungarian Sour Cherry

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3278 ◽  
Author(s):  
Andrea Nemes ◽  
Erzsébet Szőllősi ◽  
László Stündl ◽  
Attila Biró ◽  
Judit Homoki ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sour Cherry ◽  
Fresh Fruit ◽  
Water Soluble ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Trolox Equivalent

Hungarian sour cherries (SC) are excellent source of anthocyanin (concentrations (100–300 mg in 100 g fresh fruit) and melatonin (0.15 mg in 100 g fresh fruit), but other flavonoid derivatives also can be isolated by aqueous alcoholic extraction. We have developed a new process for extracting non-extractable procyanidines bound to the membrane, proteins, and fibers. These compounds were seperated with UHPLC-MS methods, and the structure of individual components were identified on the basis of their mass fragmentation spectra. The antioxidant capacity of soluble and non-soluble antioxidants were measured with ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH), trolox equivalent antioxidant capacity (TEAC) assays, and compared to the new measurement methods of water-soluble antioxidant capacity (ACW), lipid-soluble antioxidant capacity (ACL). Furthermore, total phenolic content (TPC) and total procyanidin content (PAC) were determinated. As a result of our investigation, we found that the solvent combination, where in the first step is water–ethanol (1:1), then 100% ethanol were suitable for the extraction of the extractable antioxidants. However, the chemiluminescence method that is based on the elimination of the superoxide radical is more accurate than other colorimetric methods which measure antioxidant capacity.

scholarly journals Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp

2020 ◽  
Vol 10 (17) ◽  
pp. 6007 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wen-Shin Lin ◽  
Jing-Wen Tung ◽  
Ya-Chih Cheng ◽  
Min-Yun Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Jujube Fruit

In this study, the effects of different fruit parts and extraction conditions on the antioxidant properties of jujube (Ziziphus jujuba Mill.) fruit were investigated. Five in vitro antioxidant models and statistical analyses were performed. The results revealed that jujube peel with pulp (peel pulp) exhibited better antioxidant capacity than did seeds. Overall, jujube peel pulp extracted using 50% ethanol at 60 °C exhibited the best antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (0.3 ± 0 mg/mL), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (0.5 ± 0 mg/mL), total phenolic content (38.3 ± 0.4 mg gallic acid equivalent per gram dry weight), total flavonoid content (43.8 ± 0.2 mg quercetin equivalent per gram dry weight), and reducing power (41.9 ± 2.2 mg ascorbic acid equivalent per gram dry weight). The results indicated that jujube peel pulp is a more potential natural antioxidant than seeds.

scholarly journals Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

2017 ◽  
Author(s):  
Charalampos Proestos ◽  
Theo Varzakas
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Aromatic Plants ◽  
Polyphenol Content ◽  
Stable Free Radical ◽  
Rancimat Test ◽  
Phenolic Substances

The antioxidant properties and polyphenol content of some selected aromatic plants grown in Greece were studied. Plants were refluxed with 60% methanol after acid hydrolysis. The phenolic substances were quantified by HPLC-DAD. The antioxidant capacity of the extracts was determined with the Rancimat test using sunflower oil as substrate. Free radical scavenging activity was measured using the stable free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Results were compared with standard BHT and ascorbic acid. Total phenol concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard.

scholarly journals (196) A Comparison of Fruit Quality and Antioxidant Properties of Alabama-grown Kiwi

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1050D-1050
Author(s):  
Ming-Wei S. Kao ◽  
Floyd Woods ◽  
William A. Dozier ◽  
Robert C. Ebel ◽  
Chang Y. Lee ◽  
...  
Keyword(s):  
Vitamin C ◽  
Fruit Quality ◽  
Radical Scavenging Activity ◽  
Soluble Sugars ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Health Indicators ◽  
Fresh Fruit ◽  
Kiwi Fruit ◽  
Antioxidant Power

The health status of Alabama's population ranks above the national average with respect to the prevalence of poor overall health indicators. Consumer knowledge of the health benefits of consumption of fresh fruit is lacking. The compositional and nutritional qualities of fruit are highly variable among states with different climate, soil, and other environmental conditions. Compositional and nutritional data of fresh fruit that reflect Alabama growing conditions is limited. Commercially fully ripened kiwi fruit (Actinidia deliciosa cvs. Fitzgerald and Hayward) were compared for fruit quality (pH, TA, °Brix, °Brix/TA, and soluble sugars), and antioxidant properties; Vitamin C (reduced, oxidized, and total), Vitamin C Equivalent Antioxidant Capacity (VCEAC), ferric reducing antioxidant power (FRAP), DPPH radical scavenging activity, total phenolics, and flavonoids. In general, `Fitzgerald' ranked higher in overall fruit quality and antioxidant properties when compared to `Hayward'.

scholarly journals Determination of Antioxidant Capacity, Flavonoids, and Total Phenolic Content in Eucalyptus and Clover Honeys

2014 ◽  
Vol 58 (1) ◽  
pp. 103-111 ◽  
Author(s):  
María C. Ciappini ◽  
Fernando S. Stoppani
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic

Abstract Polyphenolic compounds reportedly produce physiological effects that are beneficial to health. Bee products are particularly rich in polyphenolic compounds. We determined the antioxidant capacity and the phenolic and flavonoid compounds content of 81 samples of honey. We used the Folin-Ciocalteu reagent method to evaluate the total phenolic content. The antioxidant activities were evaluated using in vitro scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH ) and hydroxyl radicals (OH ), Trolox equivalent antioxidant capacity (TEAC ), and ferric-reducing antioxidant capacity (FRAC ). Total phenolic content ranged from 40.3 to 193.0 mg gallic acid equivalents (GAE )/100 g; the flavonoid content varied from 1.4 to 7.5 mg quercetin equivalents (QE)/100 g. Eucalyptus honeys exhibited significantly higher phenolic content and free radical-scavenging activity than clover honey samples (p<0.05 for all). Principal component analysis explained 73% of the differences observed in antiradical activity with respect to floral origin. Total phenolic content may be more useful than the radical-scavenging assay for detecting antioxidant capacity in honey; it also represents the variable that most appropriately discriminated among these honeys.

scholarly journals Antioxidant Properties of Camphene-Based Thiosemicarbazones: Experimental and Theoretical Evaluation

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1192
Author(s):  
Lijuan Yang ◽  
Haochuang Liu ◽  
Dasha Xia ◽  
Shifa Wang
Keyword(s):  
Density Functional ◽  
Peroxyl Radical ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Theoretical Data ◽  
Hydrogen Atom Transfer ◽  
Theoretical Evaluation ◽  
Wide Range

The thiosemicarbazone derivatives have a wide range of biological activities, such as antioxidant activity. In this study, the antiradical activities of six camphene-based thiosemicarbazones (TSC-1~6) were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxyl radical scavenging capacity (PSC) assays, respectively, and the results reveal that TSC1~6 exhibited good abilities for scavenging free radicals in a dose-dependent way. Compound TSC-2 exhibited the best effect of scavenging DPPH radical, with the lowest EC50 (0.208 ± 0.004 mol/mol DPPH) as well as the highest bimolecular rate constant Kb (4218 M−1 s−1), which is 1.18-fold higher than that of Trolox. Meanwhile, TSC-2 also obtained the lowest EC50 (1.27 µmol of Trolox equiv/µmol) of scavenging peroxyl radical. Furthermore, the density functional theory (DFT) calculation was carried out to further explain the experimental results by calculating several molecular descriptors associated with radical scavenging activity. These theoretical data suggested that the electron-donating effect of the diethylamino group in TSC-2 leads to the enhancement of the scavenging activities and the studied compounds may prefer to undergo the hydrogen atom transfer process.

scholarly journals Development of a new measurement method to determine plant antioxidant status

2015 ◽  
pp. 105-112
Author(s):  
Andrea Nemes ◽  
Éva Stefanovitsné Bányi ◽  
Judit Remenyik
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sour Cherry ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Reducing Ability ◽  
Trolox Equivalent ◽  
Tart Cherries ◽  
Antioxidant Effects ◽  
Soft Fruits

  The antioxidant capacity of 12 cultivar that were harvested in 2014, were determined by FRAP (Ferric Reducing Ability of Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity) TEAC (Trolox Equivalent Antioxidant Capacity) and photochemiluminescence method. In sour cherry, the most antioxidant effects of natural bioactive compounds are anthocyanins. Our results show that the photochemiluminescence method is the most suitable to determine the antioxidant capacity of red soft fruits and tart cherries

scholarly journals Effects of Blueberry and Black Carrot Extracts Addition on Antioxidant Properties and Protein-Precipitating Capacity of Ultrasound-Treated Cider

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 812
Author(s):  
Boris Brezan ◽  
Carmen Liliana Bădărău ◽  
Alexandru Woinaroschy
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Constant Frequency ◽  
Antioxidant Power ◽  
Mean Values ◽  
Black Carrot

The results presented in this study highlight the level of total phenolic content (TPC), total flavonoid content (TFC) and protein-precipitating capacity (PPC) correlated with the antioxidant capacity of new cider varieties enriched with valuable bio compounds by the addition of blueberry and black carrot extracts. The cider variants were treated with ultrasound (constant frequency of 20 kHz) at 20%, 30% and 40% amplitude (A) for two, five or seven minutes. TPC determination was performed by Folin–Ciocâlteu method and TFC was determined by an aluminum chloride colorimetric method. The antioxidant capacity was determined by ferric-reducing antioxidant power assay (FRAP), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The results showed that the antioxidant performance indicators (FRAP, ABTS and DPPH) were positively correlated with the TPC and TFC of the ultrasound-treated cider variants, suggesting that the content of these bio compounds may be improved by the treatments applied. The highest mean values for TPC and TFC were found in the cider variant enriched with black carrot extract (0.3%, v/v), subsequently ultrasound-treated (A20%, five minutes) Conclusively, the extracts used in this study could improve the antioxidant capacity of the cider, especially in case of variants ultrasound-treated.

scholarly journals CHANGES IN THE TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF FERMENTED MORINDA CITRIFOLIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 228
Author(s):  
Chaiyavat Chaiyasut ◽  
Bhagavathi Sundaram Sivamaruthi ◽  
Periyanaina Kesika ◽  
Sasithorn Sirilun ◽  
Khontaros Chaiyasut ◽  
...  
Keyword(s):  
Carbon Source ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cane Sugar ◽  
Morinda Citrifolia ◽  
Total Phenolic

Objective: The fermented plant beverages (FPBs) are considered as functional food. A detailed scientific investigation is required to develop functionally potential FPBs. The present study aimed to investigate the changes in total phenolic content and antioxidant properties of Lactobacillus paracasei HII01-mediated fermented Morinda citrifolia L. (noni) juice.Methods: The fermentation media consist of 3:10:1 ratio of noni, water, and carbon source (either cane sugar or honey) along with 10% of inoculum (L. paracasei). The control samples were prepared without inoculum or substrate. The variations in pH, acidity, total phenolic content, and the antioxidant capacity of the samples were kinetically measured by standard methods.Results: The pH and total acidity of the samples were progressively reduced and improved when the duration of fermentation was prolonged, respectively. After 15 days of fermentation, F1 (1.198 mg GAE/ml sample) and F3 (1.265 mg GAE/ml sample) exhibited high total phenolic compound compared to other samples. Likewise, sample F3 displayed maximum antioxidant capacity. The samples with cane sugar exhibited high phenolic content, free radical scavenging activity, and chelating power than samples with honey.Conclusion: About 15 days of fermentation in sufficient to obtain the high quality (rich in phenolic compounds and antioxidant capacity) fermented M. citrifolia juice using L. paracasei, and cane sugar as starter, and carbon source, respectively

scholarly journals The Comparison of the Chemical Composition, Sensory, Phenolic and Antioxidant Properties of Juices from Different Wheatgrass and Turfgrass Species

2016 ◽  
Vol 44 (2) ◽  
pp. 499-507 ◽  
Author(s):  
Abdullah ÖZKÖSE ◽  
Derya ARSLAN ◽  
Aysenur ACAR
Keyword(s):  
Vitamin C ◽  
Festuca Arundinacea ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Major Elements ◽  
Water Soluble ◽  
Total Phenols ◽  
Triticum Species

Wheatgrass juice is mainly derived from the common wheat Triticum aestivum L. The present study focused on the analysis of the potential of different perennial turfgrass species in grass juice production by determining certain compositional characteristics. The effects of fertilisers on the plants and the cutting time on some chemical constituents and antioxidant potential of grass juices were addressed. The juices from the different species of grasses, such as T. durum, T. aestivum, Lolium perenne L., Festuca arundinacea Schreb. were obtained by pressing. DPPH (1, 1diphenyl-2-picrylhydrazyl) radical scavenging activity, elemental composition, total chlorophylls, total carotenes, tocopherols, vitamin C, total phenols, viscosity and the colour profiles of the grass juice samples were analysed. Water-soluble dry matter, protein, total phenols, flavonoids and vitamin E (especially Durum cultivar) content were found to be higher in the juices of Triticum species as compared to the other grasses. Turfgrass species demonstrated higher concentrations of oil (in grass), vitamin C (unfertilised samples), chlorophyll (except L. perenne local) and major elements. The pressing of turfgrass was an easy process. Such perennial grass varieties have advantages over the Triticum species in terms of having higher concentrations of vitamin C (unfertilised samples) and major elements. The results of sensory analysis suggested that L. perenne (cultivar) is the most promising cultivar in terms of obtaining pressed grass juice.

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