scholarly journals Isolation of potential fluorescent pseudomonads from Kuini (Mangifera odorata) planted soil and their potential as biofertilizer

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Seng Heng Jeffrey Lim ◽  
Nur Samahah Mohd Zainual
Keyword(s):  
Plant Growth Promoting Rhizobacteria ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Pseudomonas Sp ◽  
Brassica Chinensis ◽  
Plant Growth Promoting ◽  
Potential Activity ◽  
Seed Germination Test ◽  
Indole 3 Acetic Acid ◽  
Soil Dilution

Pseudomonas sp. are known to be good Plant Growth Promoting Rhizobacteria (PGPR). In this study, Pseudomonas sp. were isolated from soil planted with kuini (Mangifera odorata) using soil dilution method and spread onto King’s B media. Five isolates of Pseudomonas sp. were observed to give promising results in the phytohormone and antimicrobial test conducted. These isolates are Pseudomonas sp. isolate K24pf, K29pf, K32pf, K33pf and K37pf. From the 5 potential isolates, Pseudomonas sp. isolate K29pf was chosen because it showed potential activity in producing marked amounts of Indole-3-Acetic Acid and gibberellic acid. Pseudomonas sp. isolate K29pf also produced antimicrobial activities towards Ralstonia solanacearum, Erwinia caratovora, Erwinia mallotivora and Colletotrichum gloeosporioides. Seed germination test showed that Pseudomonas sp. isolate K29pf was able to promote approximately 90% growth of Brassica chinensis seeds. Pot trial conducted showed that Treatment 3 (+OF+PGPR) was able to increase Brassica chinensis root by 36.5% and 28.4% of its biomass compared to treatment using Treatment 1 (+OF).

Rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce

1996 ◽  
Vol 42 (3) ◽  
pp. 279-283 ◽  
Author(s):  
T. C. Noel ◽  
C. Sheng ◽  
C. K. Yost ◽  
R. P. Pharis ◽  
M. F. Hynes
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Direct Growth ◽  
Early Seedling ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus cv. Westar) and lettuce (Lactuca saliva cv. Grand Rapids) was significantly promoted by inoculation of seeds with certain strains of Rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. Auxotrophic Rhizobium mutants requiring tryptophan or adenosine (precursors for indole-3-acetic acid and cytokinin synthesis, respectively) did not promote growth to the extent of the parent strain. The findings of this study demonstrate a new facet of the Rhizobium–plant relationship and that Rhizobium leguminosarum can be considered a plant growth-promoting rhizobacterium (PGPR).Key words: Rhizobium, plant growth-promoting rhizobacteria, PGPR, indole-3-acetic acid, cytokinin, roots, auxotrophic mutants.

scholarly journals Effect of Indigenous Pseudomonas sp. and Bacillus sp. Strains on Yield and Main Chemical Growth Parameters of Radicchio

2018 ◽  
Vol 67 (1) ◽  
pp. 20-26
Author(s):  
Aleksandra Stanojković-Sebić ◽  
Radmila Pivić ◽  
Zoran Dinić ◽  
Renata Iličić ◽  
Dragana Latković ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Aerial Parts ◽  
Dry Biomass ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Calcium And Magnesium

Summary Pseudomonas sp. and Bacillus sp. belong to plant growth promoting rhizobacteria which are able to colonize the plants roots and stimulate growth. In this study, the effect of two indigenous plant growth promoting rhizobacterial strains Pseudomonas sp. Q4 and Bacillus sp. Q10 and their mixture (mix Q4+Q10) on content of the main chemical growth parameters (nitrogen, phosphorus, potassium, calcium and magnesium) and the yield of dry biomass of radicchio (Cichorium spp. var. rossa di treviso) aerial parts and root, was investigated. The study was carried out with stagnosol type of soil in pot experiments under semi-controlled conditions in the Institute of Soil Science (Belgrade), in the period from July to October in 2013. Phosphorus was determined by spectrophotometer, potassium - by flame emission photometry and total nitrogen and carbon - using elemental CNS analyzer, while calcium and magnesium were determined by AAS. The data on yield of both aerial parts and root dry biomass of radicchio showed that its treatment with Q4 and Q10 strains, as well as with their mixture, caused noticeably increase in this parameter in relation to the control, whereby the strain Q4 was more effective for aerial parts, while mix Q4+Q10 - for roots. The obtained data on the studied chemical parameters of radicchio root and aerial parts were in total accordance with their yield. Concluding, studied strains have a potential in promoting the biomass yield and main chemical growth parameters of both aerial parts and root of radicchio.

scholarly journals Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi

2020 ◽  
Vol 8 (4) ◽  
pp. 590 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun
Keyword(s):  
Control Plant ◽  
Pathogenic Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
Forest Tree ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Pseudomonas Sp ◽  
Headspace Collection ◽  
Plant Growth Promoting ◽  
Encoding Gene

Plant growth-promoting rhizobacteria (PGPR) can potentially be used as an alternative strategy to control plant diseases. In this study, strain ST–TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have a strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST–TJ4 was identified as Pseudomonas sp. based on 16S rRNA-encoding gene sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1–phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Additionally, the volatile compounds released by strain ST–TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST–TJ4 obtained from headspace collection and GC–MS/MS analysis, 1-undecene was identified. In summary, the results suggested that Pseudomonas sp. ST–TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

scholarly journals Extraction of Indole-3-acetic Acid from Plant Growth Promoting Rhizobacteria of Bamboo Rhizosphere and Its Effect on Biosynthesis of Chlorophyll in Bamboo Seedlings

2020 ◽  
Author(s):  
Janardan Lamichhane ◽  
Bishnu Maya K.C. ◽  
Dhurva Prasad Gauchan ◽  
Sanjay Nath Khanal ◽  
Sharmila Chimouriya
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Biochemical Analysis ◽  
Chlorophyll Biosynthesis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Agitation Rate ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Background: Indole-3-acetic acid (IAA), a principal phytohormone, controls several crucial physiological processes of plants. It ameliorates plant growth by stimulating cell elongation, root initiation, seed germination and seedling growth. Alteration of IAA level by plant growth promoting rhizobacteria leads to varied impacts on plant growth and development. Methods: Soil samples were collected from bamboo (Bambusa tulda, B. nutans subsp. cupulata, B. balcooa and Dendrocalamus strictus) rhizosphere. Altogether five bacterial isolates were screened by serial dilution method and subjected to biochemical analysis. The isolate BUX1 with high IAA production capacity was optimized for IAA production. IAA was partially purified and quantified from the bacterial extract by thin layer chromatography (TLC). The influence of extracted bacterial IAA on chlorophyll biosynthesis in bamboo seedlings of B. tulda was compared with uninoculated control plants. Results: Biochemical analysis revealed that all the isolates belonged to genus Bacillus which were found capable of producing IAA. During optimization, BUX1 isolate produced 99.13 µg ml-1 of IAA at 37°C, pH 7, 3 mg l-1 concentration of L-tryptophan and 150 rpm agitation rate after 192 hour of incubation. The Rf value of the bacterial IAA during TLC was identical to that of standard IAA (0.425) indicating that IAA was present in crude extract of Bacillus (BUX1). The influence of bacterial IAA on chlorophyll biosynthesis in bamboo seedlings was significant in comparison to uninoculated plants. Therefore, this isolate could be a prospective candidate to be employed as biofertilizer.

scholarly journals Efficacy of rhizobacteria for growth promotion in sorghum under greenhouse conditions and selected modes of action studies

2008 ◽  
Vol 147 (1) ◽  
pp. 17-30 ◽  
Author(s):  
A. IDRIS ◽  
N. LABUSCHAGNE ◽  
L. KORSTEN
Keyword(s):  
South Africa ◽  
Agar Medium ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Chemical Fertilizers ◽  
Modes Of Action ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
Chryseomonas Luteola

SUMMARYThe screening of rhizobacteria for growth promotion of sorghum (Sorghum bicolour(L.) Moench) was conducted under greenhouse conditions for a total of 78 bacteria isolated from the rhizosphere of sorghum in Ethiopia, and 86 isolates from the rhizosphere and rhizoplane of grasses in South Africa. Three isolates from Ethiopia, all identified asBacillus cereus, enhanced growth promotion by resulting in statistically significant increases in at least five parameters. Of these,B. cereus(KBE7-8) resulted in significant increase in shoot and root biomass. Among effective isolates from South Africa,B. cereus(NAS4-3) andStenotrophomonas maltophilia(KBS9-B) showed significant increases in all the parameters measured. The isolates which resulted in significant growth promotion colonized the roots effectively with a count up to ⩾108cfu/g. In the study conducted to elucidate the possible modes of action by these effective isolates, indole 3-acetic acid-like substances were detected in culture filtrates of the isolates ranging from 4·2 μg/ml bySerratia marcescens(KBS9-R) to 22·8 μg/ml byB. cereus(KBS5-H) in the presence of 2 mg tryptophan/g nutrient broth solution. Higher rates of solubilization of tricalcium phosphate on Pikovskaya agar medium were shown byChryseomonas luteola(KBS5-F),S. marcescens(KBS6-H) andB. cereus(KBE9-1). There is very limited knowledge of the use of rhizobacteria in agriculture in Ethiopia and South Africa. The current study therefore generates valuable information towards application of plant growth promoting rhizobacteria as alternatives to chemical fertilizers.

Co-inoculation of Mesorhizobium sp. and plant growth promoting rhizobacteria Pseudomonas sp. as bio-enhancer and bio-fertilizer in chickpea (Cicer arietinum L.)

2015 ◽  
Author(s):  
Navprabhjot Kaur ◽  
Poonam Sharma ◽  
Sunita Sharma
Keyword(s):  
Synergistic Effect ◽  
Reference Strain ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pseudomonas Sp ◽  
Cicer Arietinum L ◽  
Maximum Improvement ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Pseudomonas Diminuta ◽  
Technology Programme

The present investigation was undertaken to study the synergistic effect of recommended Mesorhizobium sp. (LGR -33) (Meso) and native potential PGPR Pseudomonas sp. (PGPR 2 and PGPR 3) along with reference strain Pseudomonas diminuta (LK884) on bio-enhancing activity, symbiotic parameters and grain yield in desi (PBG 1) and kabuli (BG 1053) under field conditions in chickpea during rabi 2009-2010. Significant improvement in growth and symbiotic parameters was observed with co-inoculation of Mesorhizobium sp. with native potential Pseudomonas sp. PGPR as compared to single inoculants of Mesorhizobium sp. in both varieties. Maximum improvement in symbiotic parameters was observed with co-inoculation of Mesorhizobium sp. and PGPR 3 followed by native PGPR 2 as compared to LK884 (reference). On an average, co-inoculation of Mesorhizobium sp. with PGPR 3 improved the yield by 7.0% (desi) and 5.3% (kabuli) over Mesorhizobium sp. alone. It appears that native potential Pseudomonas sp. PGPR can be explored as potent bio-enhancers and bio-fertilizers along with Mesorhizobium sp. in chickpea under low input technology programme of sustainable agriculture.

scholarly journals Plant growth-promoting rhizobacteria associated to candelilla rhizosphere (Euphorbia antisyphilitica) and its effects on Arabidopsis thaliana seedlings

2021 ◽  
Vol 49 (2) ◽  
pp. 12294
Author(s):  
Maria T. SALAZAR-RAMÍREZ ◽  
Jorge SÁENZ-MATA ◽  
Pablo PRECIADO-RANGEL ◽  
Manuel FORTIS-HERNÁNDEZ ◽  
Edgar O. RUEDA-PUENTE ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth Promoters ◽  
Biochemical Tests ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

In the communities of Sierra Mojada and Viesca, Coahuila, Mexico of Coahuila desert, two rhizosphere samplings of candelilla (Euphorbia antisyphilitica Zucc) were collected to isolate, characterize, and identifying plant growth-promoting rhizobacteria (PGPR); 165 rhizobacteria were tested in vitro with Arabidopsis thaliana seedlings to evaluate their potential as plant growth promoters, and obtaining 21 strains with best results in the variables of the number of secondary roots and fresh weight concerning the uninoculated control. Their salinity tolerance was evaluated at concentrations from 0.85 M, 1.7 M and 2.55 M of NaCl. Biochemical tests were accomplishing such as siderophores production, phosphates solubilization, production of Indole-3-acetic acid (IAA), and the activity of the ACC deaminase enzyme. The results obtained from 21 strains selected, high activities were obtained in organic substances like a siderophores since they developed a translucent orange halo around their growth; four rhizobacteria developed a clear halo around the bacterial growth with a thickness between 1.487 mm ± 0.667 mm and 5.267 mm ± 0.704 mm in phosphates solubilization; in the production of Indole-3-acetic acid (IAA), the bacterial strains showed the presence of this phytohormone, with values ​​from 4.444 μg mL-1 to 19.286 μg mL-1; and according to the activity of the ACC deaminase enzyme, values ​​from 0.424 to 1.306 µmol α-KB/h/mg Pr were showed. 16S rRNA sequencing was carried out and genus identified were Bacillus, Staphylococcus, Acinetobacter, Cronobacter and Siccibacter. The results obtained show the potential of the isolated rhizobacteria as growth promoters and the increase in the biomass of the Arabidopsis thaliana seedlings is evident. This is a first indication to proceed to carry out tests in different phenological stages in crops of agricultural importance.

scholarly journals Investigation of salt-tolerant rhizosphere bacteria from seawater-intruding paddy rice field in Vietnam

2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Ho Tu Cuong ◽  
Bui Van Cuong ◽  
Lam Thuong Thuong ◽  
Tran Mai Hoang ◽  
Luong Thi Thu Huong ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Rice Paddy ◽  
Paddy Rice ◽  
Salt Tolerant ◽  
Plant Growth Promoting ◽  
Paddy Rice Field ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
Number Of Bacteria

Salt‐tolerant plant growth‐promoting rhizobacteria (ST‐PGPR) are known as potential tools to improve rice salinity tolerance. In this study, we aimed to investigate the plant growth‐promoting rhizobacteria community richness of the paddy rice fields in Soc Trang and Ben Tre Provinces where were seriously affected by sea level rise. The salinity in the sampling sites ranged from 0.14‰ to 2.17‰ in November 2018, the rainy season. The microbial abundance of samples was evaluated by spreading the samples in tryptic soy agar (TSA) medium supplemented with various concentrations of NaCl. With the increase of salt concentration up to 10% NaCl, a total number of bacteria decreased for all the samples, ranging from 106 to 104 CFU/g, and bacterial colonies were not observed at 30% NaCl. Among a total of 48 salt-resisting bacteria isolated from the rice paddy field mud surrounding the rice root, 22 isolates were able to produce indole-3-acetic acid (IAA: phytohormone for the plant growth). Seventeen out of 48 isolates were able to grow in the medium without nitrogen or phosphor sources. Six isolates having high IAA producing activity, nitrogen fixation and phosphate solubilization were belonged to Bacillus (DT6, LT16, and LHT8), Halobacillus (DT8), Aeromonas (LHT1), and Klebsiella (LHT7) genera. All the sequences of the strains DT6, DT8, LT16, LHT1, LHT7, and LHT8 were registered in the GeneBank with the accession numbers MK335670, MK335671, MK335672, MK335673, MK335674, and MK335675, respectively. 

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