scholarly journals Efficacy of a genotype 2 Newcastle disease vaccine (Avinew®) against challenge with highly virulent genotypes 5d and 3d

2009 ◽  
Vol 80 (3) ◽  
Author(s):  
D.G. Bwala ◽  
C. Abolnik ◽  
A. Van Wyk ◽  
E. Cornelius ◽  
S.P.R. Bisschop
Keyword(s):  
South Africa ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Disease Outbreaks ◽  
Challenge Virus ◽  
Genotype 2 ◽  
Significant Difference ◽  
Specific Pathogen ◽  
Newcastle Disease Vaccine

Since 2002, following its introduction, the lineage 5d Newcastle disease virus (so-called Goose paramyxovirus -GPMV) strain has caused numerous disease outbreaks among commercial and backyard poultry in South Africa, raising questions about the ability of commercially available Newcastle disease vaccines to fully protect poultry against the strain. This study aimed to determine whether there are differences in the level of protection offered by Avinew® Newcastle disease vaccine against GPMV virus as compared with a 3d Newcastle disease virus isolated in South Africa in 1993 (Rainbow challenge virus - RCV) strain. Six groups of 10-day-old, specific pathogen-free chickens were vaccinated with doses of 103.0, 104.5 and 106.0 EID50 of Avinew® vaccine and challenged at 4 weeks of age intramuscularly at a dose of 105.3EID50/ 0.2 mℓ/bird of GPMV and RCV. No statistically significant difference could be found in the protection offered by Avinew® vaccine against GPMV as compared to RCV challenge. The protection offered against the ND challenge was found to be dose dependent. At the recommended field dose of 106.0 EID50 the vaccine gave 100 % protection from mortality against both the challenge viruses, but not against infection and replication of the viruses, as gross lesions were evident even in apparently healthy birds that survived the challenge. The protective dose (PD90) of the Avinew® vaccine against GPMV challenge was calculated at 104.38 and against that of RCV at 104.43.

Pathogenesis of Newcastle Disease Virus Genotype VII in Chickens Vaccinated with LaSota and Inactivated Newcastle Disease Vaccines

2020 ◽  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Bursa Of Fabricius ◽  
Virus Genotype ◽  
Challenge Virus ◽  
Significant Difference ◽  
Group D ◽  
Genotype Vii

Newcastle disease (ND) is one of the most serious viral diseases affecting poultry farms in different countries. Many outbreaks -even in vaccinated poultry flocks- were recorded in the last few years caused by Newcastle disease virus (NDV) genotype VII. This study was conducted to compare the pathogenesis of NDV genotype VII in non-vaccinated chickens and chickens vaccinated with NDV genotype II live (LaSota) and inactivated vaccines. One hundred 1-day-old chicks were divided into four equal groups; 25 for each. Groups A and B were kept unvaccinated. Group C was vaccinated with LaSota, and group D was vaccinated with both LaSota and inactivated NDV vaccine. Group A was kept as nonchallenged control blank group, while groups B, C and D were challenged intranasally by 0.1 ml 106 EID50 NDV genotype VII at 25-day of age. Three chickens were sacrificed from each group at 2, 5- and 10-days post challenge (dpc). Tissue specimens from trachea, lungs, bursa of fabricius, spleen and thymus were collected for histopathology and immunohistochemistry. NDV genotype VII challenge virus did not induce mortality in both vaccinated groups. Both vaccination programs resulted also in less severe clinical signs and histopathological lesions comparing to non-vaccinated challenged birds. Tracheal lesion score was significantly low in group D at 10 dpc while no significant difference was recorded between groups C and D in lungs. All lymphoid organs showed significantly less severe pathological alterations and depletion in groups C and D comparing to group B. Our results indicated that mis-matched genotype NDV vaccines could alleviate the pathological effect of the NDV challenge virus but do not provide complete protection of the infected host organs.

scholarly journals Genome Sequences of Newly Emerged Newcastle Disease Virus Strains Isolated from Disease Outbreaks in Indonesia

2020 ◽  
Vol 9 (23) ◽  
Author(s):  
Mohammad Rabiei ◽  
Mohamad Indro Cahyono ◽  
Phuong Thi Kim Doan ◽  
Putri Pandarangga ◽  
Simson Tarigan ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Disease Outbreaks ◽  
Phylogenetic Study ◽  
Genome Sequences ◽  
Gene Coding ◽  
Study Results ◽  
Virus Strains ◽  
Genotype Vii

ABSTRACT Here, we report two genomes of newly emerged strains of Newcastle disease virus (NDV), Chicken/Indonesia/Tangerang/004WJ/14 and Chicken/Indonesia/VD/003WJ/11, from disease outbreaks in chickens in Indonesia. Phylogenetic study results of the fusion (F) protein’s gene-coding sequences of different genotypes of NDV revealed that these two strains belong to genotype VII.2 in the class II cluster of avian paramyxoviruses.

scholarly journals Ultrastructural Changes of the Tracheal Epithelium after Vaccination of Day-Old Chickens with the La Sota Strain of Newcastle Disease Virus

2005 ◽  
Vol 42 (5) ◽  
pp. 559-565 ◽  
Author(s):  
J. Mast ◽  
C. Nanbru ◽  
T. van den Berg ◽  
G. Meulemans
Keyword(s):  
Electron Microscopy ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Goblet Cells ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Ciliated Cells ◽  
Specific Pathogen ◽  
And Function

The progression of tracheal lesions induced by vaccination of day-old specific pathogen-free chicks with the La Sota strain of Newcastle disease virus (NDV) was examined by relating surface changes as observed by scanning electron microscopy with subcellular changes seen by transmission electron microscopy. NDV infection resulted in hypertrophy of goblet cells, their rupture, and the formation of excess mucus. Activation of goblet cells peaked within 4 days postvaccination. Afterward, the activation levels gradually decreased. At the level of the ciliated cells, a marked increase in the proportion of nonciliated to ciliated cells and later an almost complete deciliation of the tracheal surface were observed because a simple squamous to cuboidal epithelium replaced the original pseudostratified epithelium. Fifteen days postvaccination, all epithelial damage was restored. Because the observed vaccination-induced lesions are detrimental to epithelial integrity and function as a barrier against invading microorganisms, they might explain at the ultrastructural level the secondary complications of vaccination with the La Sota strain against NDV

scholarly journals Molecular characterisation of Newcastle disease virus exotic isolate in East Java, Indonesia

2021 ◽  
Vol 24 (2) ◽  
pp. 191-199
Author(s):  
N. P. Kusumarahayu ◽  
N. Putri ◽  
R. Ernawati ◽  
J. Rahmahani ◽  
S. Suwarno ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Rt Pcr ◽  
Genetic Characteristics ◽  
Basic Work ◽  
Specific Pathogen ◽  
Baseline Information ◽  
Native Chickens

Newcastle disease virus (NDV) is ssRNA paramyxovirus causing clinical signs, varying from subclinical infections to 100% mortality in infected chickens. Haemagglutinin-neuraminidase (HN) protein has an important role related to infection and pathogenesis, therefore, the protein was characterised in this study. Samples were collected from 45 cloacal swabs of native chickens. They were isolated by inoculating in specific pathogen-free embryonated eggs. Molecular detection of NDV was done by reverse transcriptase polymerase chain reaction (RT-PCR) encoding HN protein. RT-PCR for HN gene of NDV generated DNA fragments sized 503 bp, which were then sequenced using ABI Prism. The results have shown that virus isolates were mostly lentogenic and might contribute to outbreak in East Java, Indonesia. Based on this fact, NDV infected native chickens can act as reservoir and contribute to outbreak in the poultry. Our study provides baseline information on genetic characteristics of NDV circulating in East Java and serves as a basic work for further research.

scholarly journals Preparation and bioactivity of anti-Newcastle disease virus-phosphoprotein cytoplasmic transduction peptide antibody

2020 ◽  
Author(s):  
Benqiang Li ◽  
Man Wang ◽  
Jianguo Zhu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Protein Function ◽  
Cell Protection ◽  
Test Analysis ◽  
Hiv Tat ◽  
Significant Difference ◽  
Macromolecular Protein ◽  
And Control

Abstract On the basis of cell-penetrating peptide’s character that it can penetrate cytomembrane and transfer macromolecular protein to cytoplasm so to play biological function, we took the experiments. The fuse penetrating peptide our experiment adoptted is HIV-TAT derived fragment-CTP512, with good transmember effect and distinct cytoplasm-position. In this chapter, the research of transmembrane character was processed first. According to the tests on trans- member protein with different concentrations, the best trans-member concentration is 3µM. Afterwards, we found that the location of trans-member antibody is overlapping with phosphoprotein using indirect immunofluorescence test analysis. According to MTT test, there is no significant difference between CTP fusion protein and control on cell proliferation and viability. TCID50 test was used to detect the protective effect of trans-member antibody on cell. Result showed that trans- member antibody has significant cell protection effect compared to the control in the order: ZL.103>ZL.17>Control. Fluorogenic quantitative PCR result showed that trans- member antibody can disturb the duplication and transcription of Newcastle disease virus. This results not only paved a good way to research the transport of disease related protein, but also provide a splendid tool on protein function research.

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