Hepatoprotective activity of Ocimum sanctum alcoholic leaf extract against paracetamol-induced liver damage in Albino rats

Ethnopharmacological relevance: The dry leaf of Alchornea cordifolia (AC) is used, in traditional medicine in the S Nigeria, for the preparation of blood tonic, remedies for urinary, respiratory, liver and gas intestinal disorders. Aim of the study: This study investigated the protective property of AC leaf against liver damage in animals with a view to exploring its use for the treatment of hepatotoxicity in humans. Material and methods: Ethanol extract of A. cordifolia was used to study the hepatoprotective activity in acetaminophen-induced Albino rats (150-200g). Animals in Group 1 served as vehicle control, Group 2 served as hepatotoxin (Acetaminophen 2g/kg treated) group, Groups 3 and 4 served as positive control (Vitamin E and Curcumin 100 mg/kg bw respectively) groups, and Groups 5-8 served as (200-500mg/kg bw) AC leaf extract treated groups while Group 9 served as normal group (AC extract only 300 mg/kg bw). Results: The hepatotoxic group showed hepatocytic necrosis, cellular infiltration and inflammation in the liver. The treatment group restored the liver cells to their normal lobular architecture in a dose dependent manner. The protection offered by the plant extract compared well with the standard antioxidant agents (Curcumin and Vitamin E). Tannins, flavonoids, alkaloids and saponins were detected in the phytochemical screening. Conclusion: Our findings suggest Alchornea cordifolia ethanol leaf extract as promising herpatoprotective herb and give credence to the folkloric use of this plant for the treatment of liver problems.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10473Int J Appl Sci Biotechnol, Vol. 2(2): 217-221

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Pharmacological and Pathological Studies on Taiwan Folk Medicine (IX): The Hepatoprotective Effect of the Methanolic Extract from Echinops Grijisii

The American Journal of Chinese Medicine ◽

10.1142/s0192415x93000054 ◽

1993 ◽

Vol 21 (01) ◽

pp. 33-44 ◽

Cited By ~ 8

Author(s):

Chun-Ching Lin ◽

Cheng-Hung Lin

Keyword(s):

Liver Biopsy ◽

Ethyl Acetate ◽

Liver Damage ◽

Methanol Extract ◽

Methanolic Extract ◽

Folk Medicine ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Histopathological Changes ◽

Wistar Albino Rats

In order to isolate the main hepatoprotective component of Echinops grijisii, the crude drug was extracted with methanol and subjected to continuous extractions using n-hexane chloroform, ethyl acetate and n-butanol. The hepatoprotective studies of each fraction from the methanol extract of E. grijisii was conducted in Wistar albino rats with CC14-induced liver damage. Hepatoprotective activity was evaluated in terms of the modification of serum transaminase values such as SGOT and SGPT, and histopathological changes of liver biopsy. The results indicated that the main hepatoprotective component was concentrated in n-butanol and aqueous fractions.

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A comparative study of eugenol and Ocimum sanctum Linn. leaf extract on the antifertility effect in female albino rats

Journal of the Chinese Medical Association ◽

10.1097/jcma.0000000000000034 ◽

2019 ◽

Vol 82 (3) ◽

pp. 231-234 ◽

Cited By ~ 2

Author(s):

Venkataramanaiah Poli ◽

Changamma Challa

Keyword(s):

Comparative Study ◽

Leaf Extract ◽

Ocimum Sanctum ◽

Albino Rats

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Phytoconstituents Isolation and Hepatoprotective Activity Potential of Averrhoa bilimbi Leaf Extract

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i58b34239 ◽

2021 ◽

pp. 573-581

Author(s):

Raghavendra Prabhu ◽

Ronald Fernandes ◽

K. Adarsha Govinda

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Histopathological Examination ◽

Chemical Constituents ◽

Ethanol Extract ◽

Tween 80 ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Standard Drug ◽

Plant Chemical

Objective: To isolate and evaluate the hepatoprotective activity of the crude ethanolic leaf extract of Averrhoa bilimbi Methods: The leaves of Averrhoa bilimbi were extracted by cold maceration using ethanol as a solvent, and the solvent fractions were obtained with petroleum ether and ethyl acetate. Preliminary phytochemical tests were performed for the presence or absence of secondary metabolites. Plant chemical constituents were isolated using column chromatography and characterized by IR,1HNMR,13CNMR and mass spectroscopic values. Albino rats were treated with the vehicles (distilled water or 2% Tween 80), three different doses (100, 200 and 400 mg/kg) of the crude ethanol extract and the standard drug (silymarin 100 mg/kg), and the hepatotoxicant paracetamol. Then, the levels of biomarkers of liver injury – such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) – and liver function such as bilirubin were measured along with histopathological examination. Results: Preliminary phytochemical studies shown the presence of n-docosanoic acid and beta sitosterol from petroleum extract and from ethyl acetate a flavonoid apigenin.The ethanol extract suppressed the plasma levels of AST, ALT and ALP (P=0.05) in the aforementioned doses. Maximum hepatoprotective activity was observed at the dose of 400 mg/kg body weight. Conclusion: Averrhoa bilimbi is endowed with hepatoprotective activity, probably with the presence its chemical constituents like sterols,flavonoids and terpenoids.

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Antioxidant and Hepatoprotective Potentials of Lasimorpha senegalensis Schott Leaf Extract on Carbon Tetrachloride-induced Liver Damage in Rats

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i2130754 ◽

2020 ◽

pp. 70-78

Author(s):

Chinyere Blessing Chigor ◽

Felix Ifeanyi Nwafor ◽

Edith Ugwuja ◽

Chisimdi S. Obi

Keyword(s):

Liver Damage ◽

Leaf Extract ◽

Liver Enzymes ◽

Radical Scavenging Activity ◽

Research Work ◽

Radical Scavenging ◽

Albino Rats ◽

Endogenous Antioxidants ◽

Wistar Albino Rats

Aims: The present study assessed the antioxidant and hepatoprotective potentials of the methanolic leaf extract of Lasimorpha senegalensis – a medicinal plant used by the indigenous people of Nigeria to treat hepatitis and feverish conditions. Place and Duration of Study: The research work was conducted in the Department of Pharmacognosy and Environmental Medicine and Department of Plant Science and Biotechnology, both in the University of Nigeria, Nsukka, from May to August, 2019. Methodology: Phytochemical analyses and acute toxicity study of the sample followed standard procedures. In vitro antioxidant assay was by DPPH and H2O2 models. A total of 25 male Wistar albino rats (120 – 150 g) were grouped into five, each group with five animals. Hepatotoxicity was induced with carbontetrachloride (1 ml/kg). The treatment groups (3-5) received extract (200 and 400 mg/kg) and Silymarin (100 mg/kg). Endogenous antioxidants (superoxide dismutase, catalase, glutathione peroxidase), plasma malondialdehyde and liver enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphate) were determined after treatment. Results: The results showed the leaf extract had appreciable amounts of bioactive phytochemicals and free radical scavenging activity (IC50 of 0.52 mg/ml and 0.71 mg/ml for DPPH and H2O2 respectively) with no toxicity at 5000 mg/kg. The extract also elevated the endogenous antioxidants and significantly (p ≤ .05) reduced lipid peroxidase and liver enzymes. Conclusion: This report justifies the local use of this plant in the management of various diseases related to oxidative stress and liver damage.

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