Hepatoprotective Effect of Cassia auriculata L. Leaf Extract on Carbon Tetrachloride Intoxicated Liver Damage in Wister Albino Rats

Aims: The present study assessed the antioxidant and hepatoprotective potentials of the methanolic leaf extract of Lasimorpha senegalensis – a medicinal plant used by the indigenous people of Nigeria to treat hepatitis and feverish conditions. Place and Duration of Study: The research work was conducted in the Department of Pharmacognosy and Environmental Medicine and Department of Plant Science and Biotechnology, both in the University of Nigeria, Nsukka, from May to August, 2019. Methodology: Phytochemical analyses and acute toxicity study of the sample followed standard procedures. In vitro antioxidant assay was by DPPH and H2O2 models. A total of 25 male Wistar albino rats (120 – 150 g) were grouped into five, each group with five animals. Hepatotoxicity was induced with carbontetrachloride (1 ml/kg). The treatment groups (3-5) received extract (200 and 400 mg/kg) and Silymarin (100 mg/kg). Endogenous antioxidants (superoxide dismutase, catalase, glutathione peroxidase), plasma malondialdehyde and liver enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphate) were determined after treatment. Results: The results showed the leaf extract had appreciable amounts of bioactive phytochemicals and free radical scavenging activity (IC50 of 0.52 mg/ml and 0.71 mg/ml for DPPH and H2O2 respectively) with no toxicity at 5000 mg/kg. The extract also elevated the endogenous antioxidants and significantly (p ≤ .05) reduced lipid peroxidase and liver enzymes. Conclusion: This report justifies the local use of this plant in the management of various diseases related to oxidative stress and liver damage.

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TWIGS OF Andrographis paniculata (Burn. F) NEES ATTENUATES CARBON TETRACHLORIDE (CCl4 - ) INDUCED LIVER DAMAGE IN WISTAR ALBINO RATS

RASAYAN Journal of Chemistry ◽

10.31788/rjc.2021.1445987 ◽

2022 ◽

Vol 14 (04) ◽

pp. 2598-2603

Author(s):

Olubanke O. Ogunlana ◽

Oluseyi E. Ogunlana ◽

Jacob O. Popoola ◽

Babatunde O. Adetuyi ◽

Alaba O. Adeyemi ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Damage ◽

Andrographis Paniculata ◽

Albino Rats ◽

Wistar Albino Rats

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Hepatoprotective effect of aqueous extract of Melothria perpusilla against carbon tetrachloride induced liver injury in albino rats

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20170501 ◽

2017 ◽

Vol 5 (3) ◽

pp. 806 ◽

Cited By ~ 1

Author(s):

Nokul S. Yengkhom ◽

Ngangom Gunindro ◽

Sania M. Kholi ◽

Rakesh S. Moirangthem ◽

Bharati D. Rajkumari

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Aqueous Extract ◽

Liquid Paraffin ◽

Herbal Medicines ◽

Modern Medicine ◽

Hepatoprotective Effect ◽

Albino Rats ◽

Group Iii ◽

Group I

Background: In absence of reliable hepatoprotective drug in modern medicine, the traditional herbal medicines have been emphasized. Present study was designed to assess hepatoprotective effect of aqueous extract of Melothria perpusilla (AEMP) against carbon tetrachloride (CCl4) induced liver injury.Methods: Five groups of animals with 6 rats in each were treated for 7 days. Group I received 1% gum acacia in distilled water (1 ml/200 g p.o.) daily. Group II, III, IV and V received CCl4 in liquid paraffin (1 ml/kg s.c.) on day 2, 4 and 6. Group III, IV and V were treated respectively with silymarin (100 mg/kg p.o.), AEMP- 200 and 400 mg/kg p.o. daily. On day 8, liver injury was assessed by measuring serum ALT, AST, ALP and bilirubin.Results: ALT, AST, ALP and bilirubin were significantly reduced in groups receiving both CCl4 and AEMP when compared with CCl4 treated group.Conclusions: AEMP produced hepatoprotective effect against CCl4 induced liver injury.

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