Evaluation of petroleum ether heartwood extract of Cedrus deodara in healthy and diabetic rats

Objective: In the present study, the antidiabetic and antioxidant study of stem part of Coccinea grandis Voigt plant extracts in Streptozotocin induced diabetic rats were investigated. Materials and methods: Fifty four Wistar albino rats were used with nine groups and with six rats in each group. 45 mg/kg body weight streptozotocin was administered to group 2 to 9. Group 2 was diabetic control. Group 3 was given with glimepiride as standard drug. Group 4 and 5 were given petroleum ether extract 250 and 500 mg/kg respectively. Group 6 and 7 were given 250 and 500 mg/kg chloroform extract respectively. Group 8 and 9 were given 250 and 500 mg/kg hydro alcoholic extract respectively. Antidiabetic activity of the extracts was assessed by serum glucose level on glucose kit. Superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidation studies were assessed with histopathology. Result: The chronic study data on diabetic rats cleared the administration of all extracts significantly reduced blood glucose level and lipid peroxidation level with better antioxidant activity. Conclusion: From the study, the petroleum ether, chloroform and hydro alcoholic extracts of stem part of Coccinea grandis Voigt plant have shown antidiabetic and antioxidant potential. Key Words: Antidiabetic activity, antioxidant activity, Lipid peroxidation, Superoxide dismutase, Catalase.

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ISOLATION AND ANTIDIABETIC ACTIVITY OF NEW LANOSTENOIDS FROM THE LEAVES OF PSIDIUM GUAJAVA L.

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159//ijpps.2016.v8i9.10425 ◽

2016 ◽

Vol 8 (9) ◽

pp. 14 ◽

Cited By ~ 2

Author(s):

Priyanka Bagri ◽

Mohammed Ali ◽

Vidhu Aeri ◽

Malay Bhowmik

Keyword(s):

Metabolic Disease ◽

Petroleum Ether ◽

Silica Gel ◽

Chemical Constituents ◽

Arachidic Acid ◽

Psidium Guajava ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Soxhlet Apparatus ◽

Leaf Powder

Objective: <a href="http://www.webmd.com/diabetes/default.htm">Diabetes</a> mellitus is a chronic metabolic disease which affects our body's ability to use the energy found in food. Our study was planned to isolate chemical constituents from the leaves of Psidium guajava L. (Myrtaceae), to characterize their structures and to investigate their antidiabetic activity.Methods: The air-dried leaf powder was exhaustively extracted with methanol in a Soxhlet apparatus. The concentrated leaf extact was adsorbed on silica gel (60-120 mesh) for the preparation of a slurry. The dried slurry was chromatographed over silica gel column packed in petroleum ether. The column was eluted with petroleum ether, chloroform and methanol, successively, in order of increasing polarity to isolate the compounds. These natural constituents were tested for the antidiabetic activity in STZ-induced diabetic models.Results: Six new lanosterol-type triterpenoids characterized as lanost-7-en-3β-ol-26-oic acid (2), lanost-7-en-3β, 12β-diol-26-oic acid (3), lanost-7-en-3β, 12β, 29-triol-26-oic acid (4), lanost-cis-1,7,23-trien-3β, 12β, 18, 22α-tetraol-26-oic acid (5), lanosteryl-3β-O-D-xylopyranosyl-2′-p-benzaldehyde (7) and lanost-7-en-3β-ol-26-oic acid-3β-D-glucopyranoside (8) along with the known compounds arachidic acid (1) and β-sitosterol xylopyranoside (6) were isolated from the leaves. The compounds 2, 3, 4 and 8 exhibited significant antidiabetic activity against streptozotocin-induced diabetic rats.Conclusion: The leaves of P. guajava possessed antidiabetic lanostene-type triterpenoids.

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Chemical Composition of the Essential Oil and Petroleum Ether Extract from Korea Pine Needle Leaves of Cedrus deodara

Asian Journal of Chemistry ◽

10.14233/ajchem.2014.16543 ◽

2014 ◽

Vol 26 (10) ◽

pp. 3029-3032 ◽

Cited By ~ 1

Author(s):

Ill-Min Chung ◽

Jung -Dae Lim ◽

Bo-Ra Yu ◽

Seung-Hyun Kim ◽

Ateeque Ahmad

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Petroleum Ether ◽

Petroleum Ether Extract ◽

Ether Extract ◽

Pine Needle ◽

Cedrus Deodara

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Antidiabetic and Hypolipidemic Effects of Different Fractions of Catharanthus Roseus (Linn.) on Normal and Streptozotocin-induced Diabetic Rats

Journal of Scientific Research ◽

10.3329/jsr.v1i2.1075 ◽

2009 ◽

Vol 1 (2) ◽

pp. 334-344 ◽

Cited By ~ 15

Author(s):

M. A. Islam ◽

M. A. Akhtar ◽

M. R. Islam ◽

M. S. Hossain ◽

M. K. Alam ◽

...

Keyword(s):

Blood Glucose ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Total Cholesterol ◽

Catharanthus Roseus ◽

Triglyceride Level ◽

Serum Triglyceride ◽

Ethyl Acetate Fraction ◽

Diabetic Rats ◽

Serum Total Cholesterol

The antidiabetic and hypolipidemic effects of petroleum-ether, ethyl acetate and chloroform fractions from ethanolic extract of the leaves of Catharanthus roseus (C. roseus) were investigated in normal and streptozotocin-induced diabetic rats (SIDRs). Single doses (150 mg/kg, i.p.) of C. roseus extracts in the fasting blood glucose (FBG) levels were determined in normal and SIDRs on 0, 1, 2, 3, 6, 10, 16, and 24th hours and serum triglyceride (TG) and serum total cholesterol (TC) levels were determined after 24th hour. In normoglycemic rats and in SIDRs, petroleum-ether and ethyl acetate fraction of C. roseus reduced blood glucose level significantly. In case of hypolipidemic effects, all fractions reduced serum total cholesterol but the ethyl acetate fraction of C. roseus was the most effective. All fractions of C. roseus reduced serum triglyceride level but the ethyl acetate fraction reduced triglyceride level at the highest. The antidiabetic and hypolipidemic activities were compared to metformin HCl (150 mg/kg). Of all the three fractions, ethyl acetate fractions were the best in activity. Ethyl acetate fraction of C. roseus was found to contain flavonoids and alkaloids. The mechanism underlying the antidiabetic activity is probably increased glycogenesis, decreased gluconeogenesis or decreased absorption of glucose from intestine. Keywords: Catharanthus roseus; Antidiabetic; Hypolipidemic; Streptozotocin-induced diabetic rat.© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1075

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Anti-diabetic activity of the petroleum ether extract of Guar gum in streptozotocin-induced diabetic rats: A comparative study

Beni-Suef University Journal of Basic and Applied Sciences ◽

10.1016/j.bjbas.2013.09.008 ◽

2013 ◽

Vol 2 (1) ◽

pp. 51-59 ◽

Cited By ~ 5

Author(s):

M.R. Nasry ◽

A.M. Abo-Youssef ◽

H.A. Abd El-Latif

Keyword(s):

Comparative Study ◽

Petroleum Ether ◽

Guar Gum ◽

Petroleum Ether Extract ◽

Diabetic Rats ◽

Ether Extract

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Sites of Insulin Action Using Ferritin Labeling

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066759 ◽

1971 ◽

Vol 29 ◽

pp. 540-541

Author(s):

Burton B. Silver ◽

Ronald S. Nelson

Keyword(s):

Electron Microscopy ◽

Binding Sites ◽

Anterior Pituitary ◽

Insulin Action ◽

Diabetic Rats ◽

Insulin Antibody ◽

Fat Pad ◽

Target Organs ◽

Uranium Salts ◽

Sugar Levels

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.

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