ARTIFICIAL FREEZING OF 1-YEAR-OLD SHOOTS OF APPLE VARIETIES
One-year-old shoots of 35 apple varieties and 6 clonal rootstocks were subjected to artificial cold treatment in three winters at three dates – early winter, mid-winter, and late winter. The rate of temperature drop was 0.7 °C. per hour and a minimum temperature of −37 °C. was maintained for 8 hours. The freezing damage was determined by evaluating discoloration of tissues shortly after treatment, by estimating recovery of treated material following 3 weeks’ forcing in the greenhouse, and by electrical conductivity measurements of water extracts from twig sections. Good agreement was obtained between the results of artificial freezing obtained in early or mid-winter and the known hardiness of varieties. Treatments in late winter, i.e. February or early March, did not produce reliable results. The recovery test gave consistent results if sufficient cold injury was produced, while the electrical conductivity measurements were most valuable in differentiating fine degrees of injury. The experience and the results obtained with known varieties seem to justify the suggestion that artificial freezing may successfully be used in screening unknown plant material for frost hardiness.