A QUANTITATIVE EVALUATION OF ONE ASPECT OF FROST HARDINESS IN ALFALFA

1974 ◽  
Vol 54 (2) ◽  
pp. 343-348 ◽  
Author(s):  
P. D. WALTON

Root tissue excised from alfalfa cultivars (Medicago sativa L. and M. media Pers.) was studied to determine the relationships between impedance, living cell count, and duration of low temperature treatments. The effect of sucrose concentrations on these relationships was also examined. Higher impedances were closely associated with a high degree of cell survival. Cold-conditioning was found to increase impedance values; treatment with sucrose gave a further increase. Highly significant correlations between duration of cold treatment and mean living cell count, duration of cold treatment and impedance, and mean living cell count and impedance were found in six cultivars. Those cultivars which showed greater frost hardiness under field conditions gave higher tissue impedance values and greater cell survival in the presence of sucrose than did the frost-susceptible cultivars. The possibility of using response to sucrose treatment as a means of selecting for frost hardiness was considered.

1975 ◽  
Vol 55 (3) ◽  
pp. 823-826 ◽  
Author(s):  
P. D. WALTON

Root tissue excised from alfalfa cultivars (Medicago sativa L. and M. media Pers.) was used to determine relationships between impedance, living cell count and duration of low temperature treatment for cold-conditioned material. Correlations exist between all combinations of these three characters. For the cultivars studied, differences were found to exist between regression coefficients for the relationship between tissue impedance and living cell counts when tissue treated with sucrose was compared with tissue receiving no sucrose. The use of sucrose together with cold conditioning was also shown to be effective in detecting small differences in impedance between clones.


1979 ◽  
Vol 15 (10) ◽  
pp. 282 ◽  
Author(s):  
Mariano Aceves ◽  
L. don Kendall

2008 ◽  
Vol 198 (6) ◽  
pp. 843-850 ◽  
Author(s):  
Sarah W. Read ◽  
Richard A. Lempicki ◽  
Michele Di Mascio ◽  
Sharat Srinivasula ◽  
Rosanne Burke ◽  
...  

2021 ◽  
Vol 6 (1) ◽  
pp. 78-86
Author(s):  
N. Yu. Moskalenko ◽  
O. A. Kudryashova ◽  
L. S. Kudryashov ◽  
S. L. Tikhonov ◽  
N. V. Tikhonova ◽  
...  

It is known that processing methods ensuring partial or full microbial inactivation are quite limited. Therefore, it is of great interest to develop technique and technologies allowing the effective action on microorganisms without a significant influence on product properties. The use of cold plasma can be one of the promising methods of meat product treatment by cold sterilization. The present work examines a possibility of chilled meat treatment with low-temperature atmospheric-pressure plasma to increase its stability to microbial spoilage and extend shelf life. To obtain low temperature plasma, the equipment developed by the designing department “Plasmamed” was used. Chilled meat was treated with low-temperature atmospheric-pressure argon plasma for 5, 10, 20 and 30 min. Samples were stored at a temperature of 2–4 °C for 10 days. Organoleptic indices, moisture weight fraction, changes in pH and water activity were analyzed before treatment and during storage. Sanitary microbiological analyses were carried out by the following indicators: quantity of mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM), the presence and quantity of coliforms, Salmonella, Escherichia coli, Listeria monocytogenes, Proteus. It was shown that meat cold treatment with argon plasma inhibited the development of mesophilic microorganisms. The colony forming units detected in the samples after ten days of storage were determined by the duration of exposure to plasma. It was proved that meat treatment for 15 and 30 min had the bactericidal effect and facilitated an improvement in meat color during storage. The organoleptic indices of the samples treated with plasma corresponded to the requirements of standards and approved consumer characteristics.


1983 ◽  
Vol 10 (6) ◽  
pp. 539 ◽  
Author(s):  
DH Greer

The development of frost hardiness in seedlings of P. radiata was followed using a photoperiod of 9 h and a constant day temperature of 12°C. The seedlings were preconditioned to a night temperature of 6.5°C for 35 days and 4°C for 7 days. The temperature was then reduced to -4°C for 66 days in one hardening treatment (designated 12/-4°C) and to 3°C for 136 days in another (designated 12/3°C). At the end of those periods, the night temperature was increased back to 6.5°C for a further 15 days. Frost hardiness was determined at regular intervals by exposing the seedlings to a series of artificial frosts. During the low temperature regime, frost hardiness developed continuously in seedlings from both treatments, from -5 to -9.5°C in the 12/3°C treatment and to -16.5°C in the 12/-4°C treatment. The rate of development of frost hardiness was constant at constant temperatures. Using a temperature response model, specific rates of hardening were estimated for both this and an earlier experiment, and found to be approximately linear against temperature. The analysis establishes that temperature controls the hardening process of P. radiata by regulating the rate of development of frost hardiness. Frost hardiness was also shown to be related to thermal time. Before this relationship could be useful in predicting frost hardiness of field-grown seedlings, the relative contribution of the photoperiod-induced and temperature-induced hardening in natural environments needs to be more clearly defined.


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