LC–MS/MS quantification of asymmetric dimethyl arginine and symmetric dimethyl arginine in plasma using surrogate matrix and derivatization with fluorescamine

Bioanalysis ◽  
2020 ◽  
Vol 12 (22) ◽  
pp. 1607-1619
Author(s):  
Venkatraman Junnotula ◽  
Barry R Jones ◽  
Shelby Gorman ◽  
Miaoqing Shen ◽  
Daniel Mulvana
Keyword(s):  
Protein Precipitation ◽  
Analytical Methodology ◽  
Internal Standards ◽  
Simultaneous Quantification ◽  
Preclinical Species ◽  
Surrogate Matrix ◽  
Asymmetric Dimethyl Arginine ◽  
Dimethyl Arginine ◽  
Positive Ion

Aim: A novel LC–MS/MS method using a surrogate matrix and derivatization with fluorescamine was developed and validated for simultaneous quantification of asymmetric dimethyl arginine and symmetric dimethyl arginine. Methods & results: Asymmetric dimethyl arginine, symmetric dimethyl arginine and corresponding internal standards were extracted using protein precipitation and derivatization with fluorescamine followed by SPE. Derivatives were analyzed by turbo ion spray LC–MS/MS in the positive ion mode. Methodology was successfully transferred across multiple preclinical species and utilized in the support of several investigative studies. Conclusion: A new LC–MS/MS analytical methodology that utilizes a surrogate matrix and derivatization with fluorescamine was successfully developed and validated.

scholarly journals The Development and Validation of a Novel Designer Benzodiazepines Panel by LC–MS-MS

2021 ◽  
Author(s):  
Rebecca A Mastrovito ◽  
Donna M Papsun ◽  
Barry K Logan
Keyword(s):  
Illicit Drug ◽  
Liquid Extraction ◽  
Electrospray Tandem Mass Spectrometry ◽  
Method Performance ◽  
Internal Standards ◽  
Liquid Liquid Extraction ◽  
Relative Standard ◽  
Development And Validation ◽  
Positive Ion ◽  
Designer Benzodiazepines

Abstract Novel illicit benzodiazepines are among the most active areas of new illicit drug manufacture and use. We describe a method for the detection and quantification of etizolam and its metabolite α-hydroxyetizolam, flubromazolam, clonazolam, diclazepam, delorazepam, bromazepam, flubromazepam, phenazepam, flualprazolam, flunitrazolam, and nitrazolam in human whole blood. After addition of internal standards, samples are buffered and extracted using a liquid–liquid extraction. Analysis is performed using positive-ion electrospray tandem mass spectrometry for detection and quantitation. Calibration ranges were established based on the method performance and differed from compound to compound. Replicates at the lowest calibration point for each compound performed within 5% of CV (Coefficient of Variation). The correlation coefficient was >0.990 for all compounds. Relative standard deviation for all compounds was ≤10% of CV and accuracy was  ±10% for both within- and between-run experiments. The maximum average intra- and inter-run imprecision were 5.7%. The maximum average intra- and inter-run imprecision was −8.7%. As part of evaluating the scope for relevancy, samples testing positive in immunoassay but confirmed to be negative in traditional benzodiazepine confirmation method were re-analyzed using this method. The presence of at least one novel benzodiazepine was identified in 70% of these samples. The appearance of these novel “designer” benzodiazepines demonstrates the challenge for toxicology testing and the need for continually updated confirmation methods.

The association between serum asymmetric dimethyl arginine levels and a history of gestational diabetes among healthy women

2012 ◽  
Vol 23 (5) ◽  
pp. 391-395 ◽  
Author(s):  
Ilknur I. Gumus ◽  
Ayse Kargili ◽  
Ikbal Kaygusuz ◽  
Aysel Derbent ◽  
Feridun Karakurt ◽  
...  
Keyword(s):  
Gestational Diabetes ◽  
Healthy Women ◽  
History Of ◽  
Asymmetric Dimethyl Arginine ◽  
Dimethyl Arginine

Asymmetric dimethyl arginine levels in testicular tissue and blood of rats with induced experimental varicocele

2014 ◽  
Vol 12 (4) ◽  
pp. 139-144
Author(s):  
Cabir Alan ◽  
Yunus Ertung ◽  
Dilek Ulker Cakır ◽  
Naci Topaloglu ◽  
Ahmet Resit Ersay ◽  
...  
Keyword(s):  
Testicular Tissue ◽  
Asymmetric Dimethyl Arginine ◽  
Dimethyl Arginine

scholarly journals Validation of an LC-MS/MS Method for the Quantification of Caffeine and Theobromine Using Non-Matched Matrix Calibration Curve

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2863 ◽  
Author(s):  
Vera M. Mendes ◽  
Margarida Coelho ◽  
Angelo R. Tomé ◽  
Rodrigo A. Cunha ◽  
Bruno Manadas
Keyword(s):  
Human Plasma ◽  
Calibration Curve ◽  
Considerable Effort ◽  
Caffeine Consumption ◽  
Internal Standards ◽  
Beneficial Effects ◽  
Calibration Curves ◽  
Accuracy And Precision ◽  
Precise Quantification ◽  
Surrogate Matrix

Caffeine is one of the most widely consumed psycho-stimulants. The study of the beneficial effects of caffeine consumption to decrease the risk of developing several neuropsychiatric pathologies is receiving increasing attention. Thus, accurate and sensitive methods have been developed, mainly by LC-MS/MS, in order to quantify caffeine and its metabolites. These quantifications of caffeine and its metabolites by LC-MS/MS require a considerable effort to select or find a surrogate matrix, without the compounds of interest, to be used in the calibration curves. Thus, we evaluated the possibility of using calibration curves prepared in solvent instead of calibration curves prepared in human plasma. Results show that the calibration curves prepared in solvent and in human plasma were similar by comparing their slopes and interceptions, and the accuracy and precision were within the limits of acceptance for both calibration curves. This work demonstrates that, by using internal standards, it is possible to use a calibration curve in solvent instead of a calibration curve in plasma to perform an accurate and precise quantification of caffeine and theobromine.

scholarly journals Asymmetric Dimethyl Arginine Level in Children with Sickle Cell Disease: A Cross Sectional Study

2021 ◽  
pp. 93-98
Author(s):  
Seham Fathy Khedr ◽  
Mohamed Hosny El Bradaey ◽  
Hala Mohamed Nagy ◽  
Mohamed Ramadan El-Shanshory ◽  
Eslam Elhawary
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cell Disease ◽  
Adma Level ◽  
Cross Sectional ◽  
Asymmetric Dimethyl Arginine ◽  
Dimethyl Arginine

Background: Sickle cell disease (SCD) consists of a group of hemoglobinopathies in which individuals inherit hemoglobin variants derived from single point mutations. Asymmetric dimethylarginine (ADMA) contributes to limiting Nitric Oxide (NO)  bioavailability in SCD. The aim of the present study was to assess the level of the Asymmetric Dimethyl Arginine in children with sickle cell. Methods: This cohort cross-sectional study was carried out on 60 children which were divided in to 3 equal groups. Group I: SCD children with sickle retinopathy. Group II: SCD children without retinopathy. Group III: healthy control children who were selected from the outpatient clinic. Results: There was a significant increase in ADMA level among participants withSCD. There was a positive significant correlation between ADMA  level and family history as well as the  incidence of hepatomegaly. There was no significant correlation between ADMA level and demographic and laboratory parameters except LDH. Conclusions: The level of ADMA is elevated in children with sickle cell anemia. High plasma ADMA level is a risk for hepatomegaly in children with sickle cell anemia.

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