scholarly journals Determination of Phenol Content and Antibacterial Activity of Five Medicinal Plants Ethanolic Extracts from North-West of Morocco

Author(s):  
Abdelhakim Bouyahya
2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Bishnu P. Marasini ◽  
Pankaj Baral ◽  
Pratibha Aryal ◽  
Kashi R. Ghimire ◽  
Sanjiv Neupane ◽  
...  

The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find thein vitroantibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range ofin vitrogrowth inhibitory action against the tested bacterial species, andCynodon dactylonwas found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistantStaphylococcus aureus, imipenem-resistantPseudomonas aeruginosa, multidrug-resistantSalmonella typhi, andS. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts ofCinnamomum camphora, Curculigo orchioides, andCurcuma longaexhibited the highest antibacterial activity againstS. pyogeneswith a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction ofCynodon dactylonexhibited best antibacterial activity againstS. aureuswith a MIC of 31 μg/mL. Among all,C. dactylon, C. camphora, C. orchioides, andC. longaplant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.


1970 ◽  
Vol 18 ◽  
pp. 128-133 ◽  
Author(s):  
MTH Molla ◽  
MS Ahsan ◽  
MT Alam ◽  
ME Haque

Context: Development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. Seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study.   Objectives: Evaluation of the effectiveness of some medicinal plant extracts against four Gram-positive and five Gram-negative bacteria.  Materials and Methods: The antibacterial activity of the crude ethanolic extracts obtained from the leaves of seven medicinal plants; viz., Andrographis paniculata, Catharanthus roseus, Adhatoda vasica, Vitex vegundo, Aloe vera, Flacortia ramontchi and Nyctanthes arbortristis were tested against nine bacteria at concentrations of 300-, 400- and 500 μg/ml. Standard antibiotic disc kanamycin (30μg/ml) was used for comparison. The minimum inhibitory concentration (MIC) of ethanolic extracts of the leaves of these medicinal plants were determined by testing the extracts on four Gram-positive and five Gram-negative bacteria by serial tube dilution method.   Results: All the extracts have notable antimicrobial activities against the test organisms. The ethanolic extracts of the leaves showed the highest antimicrobial activities against Bacillus megaterium and Shigella dysenteriae for An. paniculata, Ad. vasica and Al. vera; Bacillus subtilis and Salmonella typhi for C. roseus and N. arbortristis; Staphylociccus aureus and Salmonella typhi for V. vegundo; and Bacillus subtilis and Shigella sonnei for F. ramontchi respectively. The extract of the plants had MIC values ranging from 32 to 128 mg/ml. All plant extracts showed no MIC against Shigella shiga and against Sarcina lutea only C. roseus showed MIC 128 mg/ml.   Conclusion: The results revealed that the ethanolic extracts of the plants under present investigation have notable antimicrobial activities.   Keywords: medicinal plants; antimicrobial screening; MIC; bacteria. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8788 JBS 2010; 18(0): 128-133


2021 ◽  
Vol 10 (5) ◽  
pp. e7510514493
Author(s):  
Selene Maia de Morais ◽  
Francisco Flávio da Silva Lopes ◽  
Gilson Araújo Fontenele ◽  
Marcus Vinícius Ferreira da Silva ◽  
Victor Borges Fernandes ◽  
...  

The State’s Cocó Park in the city of Fortaleza-CE present mainly a mangrove flora and include landscape and medicinal plants. The aim of this study is determining the total phenol content, antioxidant activity against the free radical DPPH and the inhibition of the enzyme acetylcholinesterase (AChE) in ethanolic extracts of 30 medicinal plants and thus assess which plants have potential against Alzheimer's Disease.  The plants rich in phenolic compounds with amounts ranging from 297.46 ± 26.94 to 599.30 ± 17.08 mg GAE/g plant extract, which showed greater antioxidant activities (with IC50 against DPPH radical from 3.44 ± 0.16 to 3.73 ± 0.12 µg mL-1) and higher acetylcholinesterase inhibiting power (IC50 < 20 μg mL-1) were Anacardium occidentale, Ceiba pentandraLaguncularia racemosa, Mangifera indica, Myracrodrum urundeuva and Terminalia catappa. Then, these species and their constituents are recommended for more specific studies related to Alzheimer´s Disease.


2017 ◽  
Author(s):  
Luong Thi My Ngan ◽  
Pham Phuong Dung ◽  
Nguyen Vang Thi Yen Nhi ◽  
Nguyen van Minh Hoang ◽  
Tran Trung Hieu

Author(s):  
A. Megalinska ◽  
M. Sokulska

The article is devoted to the important problem of the search of herbal preparations with high antibacterial, cytostatic and lithic activity. The plants such as Ambrosia artemisiifolia, which belongs to the family of Aystrou, are promising in this direction. The aim of the presented work is to study the antibacterial, cytostatic and lithic activity of water extracts of Ambrosia polynolithic and conduct a comparative analysis of these properties with other medicinal plants. Determining that the lectin extract from the green shoots (until flowering) of Ambrosia artemisiifolia does not agglutinate red blood cells, makes it possible to consider this raw material as a potential drug in pharmacology. Investigation of cytostatic activity by the method of Ivanov and Bystrova enabled to establish a place of ambrosia among already well-known medicinal plants. The cytostatic activity of the investigated plants can be represented by the following series in the order of reduction: Chelidónium május, Ambrosia artemisiifolia, Vinca minor, Viscum album. The establishment of antibacterial activity allowed to confirm the presence of anti-staphylococcal activity of the water extract of ambrosia and its significant effect on the colon. Anti-staphylococcus activity of the water extract of Ambrosia artemisiifolia is close to Viscum album and Chelidónium május. The antibacterial effect against Proteus vulgaris was the highest in the extract of аmbrosiа shoots, compared with other medicinal plants studied. Taking into account the phylogenetic method, it could be expected that the plants of the Aystroe family may have a lithic activity with respect to the concrements that are formed in the kidneys of man. So, a study was conducted on the lithic activity of polystyrene embryos and it was found that Ambrosia artemisiifolia has a high lithic activity in relation to urate and oxalate concretions.


2017 ◽  
Vol 5 (1) ◽  
pp. 62 ◽  
Author(s):  
Stephen Enabulele ◽  
Oluwatosin Amusa ◽  
Eseosa Uwadiae

The leaves of two medicinal plants namely; Morinda lucida and Nauclea latifolia were investigated for their antibacterial, mineral and phytochemical properties. Results of the study showed that the aqueous and ethanolic extract of the two leaves had significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes and Proteus mirabilis. The biologically active constituents present in the extracts were cardiac glycosides, tannins, saponin, coumarins, reducing sugar, terpenoids, alkaloids, steroids and flavonoids. The percentage yield extracts of the respective plants were Morinda lucida leaves 12.9% for ethanol and 9.0% for aqueous extracts while Nauclea latifolia had 12.1% for ethanol and 8.4% for aqueous extracts. Results of the antibacterial activity analysis revealed that the ethanolic extracts of the two medicinal plants at different concentrations were more active against the test organisms named above than the aqueous extracts. The MIC values for the ethanolic extracts ranged between 25.0 and 100.0 mg/ml while that of aqueous extract ranged between 50.0 and 100.0 mg/ml. The MBC values for ethanolic extract ranged between 50.0 and 100mg/ml while that of aqueous extracts also ranged between 50.0 and 100mg/ml. Analysis of the mineral content of the plant leaves shows that they were rich in potassium and calcium. This study confirms that the leaf extracts have some reasonable level of antimicrobial activity and appreciable amounts of mineral content which could be beneficial to human health. The findings from this work can be further exploited for isolation and characterization of novel Phytochemical compounds which could be used in the treatment of infectious diseases especially in light of the emergence of drug-resistant microorganisms and the need to produce new effective antimicrobial agents.


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