Evaluation of Methyl Red Tolerant Cyanobacteria for Simultaneous Laccase Production and Dye Decolorization

2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Mohd Kafeel Ahmad Ansari ◽  
Umar Mohteshum Khatib
Keyword(s):  
Dye Decolorization ◽  
Laccase Production ◽  
Methyl Red

scholarly journals Evaluation of Enzymes Production Activity of Trichoderma, Aspergillus and Rhizopus Species in Paraeforce (Herbicide) Degradation

2020 ◽  
pp. 1-7
Author(s):  
F. I. Onianwah ◽  
H. O. Stanley ◽  
V. C. Eze ◽  
V. O. Ifeanyi ◽  
C. J. Ugboma
Keyword(s):  
Hydrogen Peroxide ◽  
Soil Samples ◽  
Laccase Production ◽  
Methyl Red ◽  
Aerobic Incubation ◽  
Fungal Isolation ◽  
Qualitative And Quantitative ◽  
Herbicide Degradation ◽  
Production Activity ◽  
Catalase Peroxidase

The study aims to evaluate enzymes that facilitate fungal degradation of paraeforce. Soil samples for fungal isolation were collected from impacted sites and inoculated on potato dextrose agar (PDA). The isolates were screened for growth and tolerance to paraefoce in 50 mg/l concentration of the test herbicides. Trichoderma, Aspergillus and Rhizopus species were found to grow in paraeforce supplemented PDA. Qualitative and quantitative assay for different enzyme production in hydrogen peroxide, methyl red, guaiacol and hydrogen peroxide-pyrogallol complex proved potential for catalase, lignin peroxidase, laccase and manganese peroxidase production, respectively. The results showed that these three fungi have great potential for catalase, peroxidase and laccase production after six days aerobic incubation in paraeforce and these enzymes facilitated the utilization of the paraeforce.  

scholarly journals Laccase production by Galerina sp. and its application in dye decolorization

2014 ◽  
Vol 5 (2) ◽  
pp. 13-22 ◽  
Author(s):  
Mendoza Laura ◽  
Ibrahim Victor ◽  
Teresa lvarez Maria ◽  
Hatti-Kaul Rajni ◽  
Mamo Gashaw
Keyword(s):  
Dye Decolorization ◽  
Laccase Production

Production of spore laccase from Bacillus pumilus W3 and its application in dye decolorization after immobilization

2017 ◽  
Vol 76 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Wen Zhou ◽  
Zheng-Bing Guan ◽  
Yu Chen ◽  
Feng Zhang ◽  
Yu-Jie Cai ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Textile Wastewater ◽  
Dye Decolorization ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Laccase Production ◽  
Methyl Green ◽  
Liquid Loading ◽  
Bacillus Genus ◽  
Acid Red 1

Given that spore laccase from the Bacillus genus is heat- and alkali-resistant, it is more suitable for industrial applications than fungal laccase. To determine the optimal culture conditions for spore laccase production, the effects of Cu2+ concentration, oxygen content, and culture time on spore laccase production from Bacillus pumilus W3 were investigated. The optimal production parameters were 0.2 mM of Cu2+, 200 rpm shaking speed, 100 mL liquid loading, and 5 days of cultivation. Spore laccase was efficiently immobilized on amino-functionalized celite. When used in dye decolorization, the immobilized spore laccase removed 84.15% of methyl green and 69.70% of acid red 1 after 48 h of treatment. Moreover, the immobilized spore laccase retained 87.04% of its initial decolorization activity after six cycles in the decolorization of acid red 1. These insights into the culture conditions and immobilization of spore laccases should be useful in the development of spore laccase as a biocatalyst in the treatment of textile wastewater.

scholarly journals Study of suitable media for laccase synthesis of basidiomycete fungi from Bidoup - Nuiba park and its reactive dye decolorization ability

2016 ◽  
Vol 14 (2) ◽  
pp. 313-325
Author(s):  
Trần Thị Thu Hiền ◽  
Lê Thị Hiền ◽  
Nguyễn Văn Huynh ◽  
Đinh Thị Thu Hằng ◽  
Đặng Thị Cẩm Hà
Keyword(s):  
Dye Removal ◽  
Dye Decolorization ◽  
Reactive Dye ◽  
Polycyclic Aromatic Compounds ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Its Sequence Analysis ◽  
Polycyclic Aromatic ◽  
Acid Blue ◽  
Basidiomycete Fungi

A fungal strain FBD154 with high laccase production was isolated from Bidoup-Nui Ba Parks, Lam Đong, Vietnam. FBD154 was identified as Polyporus sp. FBD154 based on traditional method and ITS sequence analysis. Polyporus sp. FBD154 synthesized laccase with high activity 74925 U/l on modified TSH1 (200 g/l containing potato extract, 1 g/l casein, 1 g/l rice bran, 5 g/l soybean meal, 10 g/l manose, 3 g/l NH4Cl, 0.3 mM CuSO4, pH 4). Crude laccase of Polyporus sp. FBD154 was applied to decolor several synthetic and commercial dyes. After 30 min, laccase from Polyporus sp. FBD154 could decolorize synthetic dyes at 100 mg/l concentration with efficiency of 60% acid red (NY1), 18% acid red 299 (NY7), 52% acid blue 281 (NY5) and 83% Remazol Brilliant Blue R (RBBR) without mediator. For commercial dyes at concentration of 100 mg/l, color removal efficiency reached to 62% megafix black CLS (CLS) and 72% everzol red LF2B (LF-2B) without mediator. Efficiency of synthetic dye removal by Polyporus sp. FBD154 crude laccase with 200 µM mediator obtained 90% RBBR with violuric acid (VIO) after 30 min; 80% NY5 with hydroxybenzotriazole (HBT) for 24 hours; 87% NY1 with acetosyringone (Ace) for 5 min; 92% NY7, 91% LF-2Band 73% CLS with syringaldehyde (Syr) for 5 min. The obtained evidences show that laccase was synthesized by Polyporus sp. FBD154 with high potential for application in wastewater treatment of textile plants in particular as well a sin detoxification of polycyclic aromatic compounds in general.

scholarly journals Biological Consortia Designed for Laccase Production and Dye Removal

2018 ◽  
Author(s):  
Roberto González ◽  
Roberto Villagómez ◽  
Alfredo Madariaga ◽  
Javier Castro ◽  
César González
Keyword(s):  
Azo Dyes ◽  
Dye Removal ◽  
Dye Decolorization ◽  
The Other ◽  
Laccase Production ◽  
Attractive Alternative ◽  
Textile Effluents ◽  
The Poor ◽  
Enzyme Isoforms ◽  
Low Efficiency

The potential of biological consortia designed for laccase production and dye treatment is discussed in this review. The poor yields in laccase production and low efficiency in dye decolorization of monoculture-based treatments has encouraged the use of designed biological consortia. A consortium is a system where the growth of two or more organisms, chosen to improve a particular bioprocess, is induced in the same medium. Chemical and natural mediators are being replaced by consortia for the production of laccases because, in addition to being less toxic, they induce new enzyme isoforms and lead to high laccase yields. On the other hand, consortia act synergistically in the decolorization of azo dyes through the enzymes they produce, so overall degradation is improved. Designed consortia are an attractive alternative still in development that could provide new biotechnological tools for the treatment of textile effluents.

scholarly journals Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes

2020 ◽  
Vol 6 (4) ◽  
pp. 301
Author(s):  
Ivana Eichlerová ◽  
Petr Baldrian
Keyword(s):  
Enzyme Production ◽  
Ligninolytic Enzymes ◽  
Brown Rot ◽  
Dye Decolorization ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Mn Peroxidase ◽  
Taxonomic Groups

An extensive screening of saprotrophic Basidiomycetes causing white rot (WR), brown rot (BR), or litter decomposition (LD) for the production of laccase and Mn-peroxidase (MnP) and decolorization of the synthetic dyes Orange G and Remazol Brilliant Blue R (RBBR) was performed. The study considered in total 150 strains belonging to 77 species. The aim of this work was to compare the decolorization and ligninolytic capacity among different ecophysiological and taxonomic groups of Basidiomycetes. WR strains decolorized both dyes most efficiently; high decolorization capacity was also found in some LD fungi. The enzyme production was recorded in all three ecophysiology groups, but to a different extent. All WR and LD fungi produced laccase, and the majority of them also produced MnP. The strains belonging to BR lacked decolorization capabilities. None of them produced MnP and the production of laccase was either very low or absent. The most efficient decolorization of both dyes and the highest laccase production was found among the members of the orders Polyporales and Agaricales. The strains with high MnP activity occurred across almost all fungal orders (Polyporales, Agaricales, Hymenochaetales, and Russulales). Synthetic dye decolorization by fungal strains was clearly related to their production of ligninolytic enzymes and both properties were determined by the interaction of their ecophysiology and taxonomy, with a more relevant role of ecophysiology. Our screening revealed 12 strains with high decolorization capacity (9 WR and 3 LD), which could be promising for further biotechnological utilization.

scholarly journals Molecular phylogeny of wood decay fungi of hardwood and their ability to produce laccase that correlates with triphenylmethane dye decolorization

2019 ◽  
Author(s):  
Maduri Piumi Sashikala Mahawaththage Dona ◽  
Anushi Suwanethya Deraniyagala ◽  
Priyanga Wijesinghe ◽  
Renuka Nilmini Attanayake
Keyword(s):  
Sri Lanka ◽  
Fusarium Solani ◽  
Wood Decay ◽  
Dye Decolorization ◽  
Fungal Species ◽  
Laccase Production ◽  
Decay Fungi ◽  
Triphenylmethane Dye ◽  
Hardwood Species ◽  
Dry Zone

AbstractThough Sri Lanka belongs to one of the 34 biodiversity hotspots of the world, its microfolora specially fungi are not well studied and underrepresented in the global literature. Here we report the fungal species diversity of decaying hardwood of a Sri Lankan dry zone forest for the first time. Decaying hardwoods were collected from historically important Dimbulagala forest reserve, Sri Lanka and fungi associated with these woods were isolated. Out of 35 fungal species identified using morphological and molecular methods, 11 species were first records in Sri Lanka. All the tested isolates were able to utilize wood as the sole carbon source and produced varying degrees of laccase. Isolates of Perenniporia tephropora, Coriolopsis caperata, Gymnopilus dilepis, Fusarium solani and Vanderbylia fraxinea were among the top six laccase producers. Except Fusarium solani, the rest of the isolates showed more than 70% decolorization of the of triphenylmethane dye and there was a significant positive correlation between laccase production and dye decolorization. To the best of our knowledge laccase production and dye decolorization ability of Vanderbylia fraxinea and Gymnopilus dilepis have never been reported in the fungal kingdom before. Perenniporia tephropora was isolated from one of the strongest decay resistant hardwood species, Ebony (Diospyros ebenum) also known as dark wood and V. fraxinea was isolated from another medicinally important hardwood Neem (Azadirachta indica). Findings of this study confirms that decaying hardwood of Sri Lanka provide unexplode a unique niche for discovering fungal species with biotechnological applications such as high laccase producers and dye decolorizers.

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