Vitamin D Receptor Genetic Variants among CKD Patients of South Indian Population

Abstract Background Diabetic nephropathy is known to be a leading complication of diabetes mellitus, characterized by diverse aspects such as high urinary albumin level, elevated blood pressure, and genetic susceptibility leading to end-stage renal disease. The current study was carried out to investigate the association of eNOS and TGFβ1 gene polymorphisms in the progression of diabetic nephropathy among type 2 diabetic patients in the South Indian population. The eNOS and TGFβ1 genetic variants were genotyped in 280 T2DM patients, 140 with DN, 140 without DN, and 140 controls. Genotyping was performed using ARMS PCR and the genomic variants were confirmed by the Sanger sequencing method. Results A significant (p < 0.05) association was observed in the genotypic frequencies of eNOS (G > T) polymorphism in the T2DM patients with diabetic nephropathy when compared to controls. The frequency of TT (heterozygous) genotype was observed to increase in patients with type 2 diabetes and DN when compared to the diabetic patients without DN and controls. This indicates that diabetic patients with TT genotype are at an increased risk to develop DN. However, TGFβ1 (G > C) polymorphism did not show any association in the allele and genotypic frequencies with DN when compared with T2DM and controls. Conclusion The results of the study propose a strong influence of TT genotype of eNOS gene be significantly linked with diabetic nephropathy in T2DM patients. Whereas no association was examined concerning TGFβ1 gene polymorphism and DN. Nevertheless, large sample size studies are required to confirm the part of these genetic variants in the development of DN.

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Prevalence of vitamin D deficiency in urban south Indians with different grades of glucose tolerance

British Journal Of Nutrition ◽

10.1017/s0007114520001129 ◽

2020 ◽

Vol 124 (2) ◽

pp. 209-216 ◽

Cited By ~ 1

Author(s):

Ramamoorthy Jayashri ◽

Ulagamathesan Venkatesan ◽

Coimbatore S. Shanthirani ◽

Mohan Deepa ◽

Ranjit Mohan Anjana ◽

...

Keyword(s):

Vitamin D ◽

Glucose Tolerance ◽

Vitamin D Deficiency ◽

Indian Population ◽

Vitamin D Supplementation ◽

South Indian Population ◽

The Metabolic Syndrome ◽

South Indian ◽

Urban South ◽

Vitamin D Levels

AbstractThe present study assessed the prevalence of vitamin D deficiency in an urban south Indian population in individuals with different grades of glucose tolerance. A total of 1500 individuals (900 normal glucose tolerance (NGT), 300 prediabetes and 300 with type 2 diabetes mellitus (T2DM)) who were not on vitamin D supplementation were randomly selected from the Chennai Urban Rural Epidemiological Study follow-up study. Anthropometric, clinical examination and biochemical investigations (25-hydroxyvitamin D (25(OH)D), insulin, glycated Hb (HbA1c) and serum lipids) were measured. Vitamin D deficiency was defined as serum 25(OH)D < 20·0 ng/ml, insufficiency as 20–29·9 ng/ml and sufficiency as ≥30 ng/ml. Of the 1500 individuals studied, 45 % were males and the mean age was 46 (sd 12) years. Vitamin D levels lowered with increasing degrees of glucose tolerance (NGT: 21 (sd 11); prediabetes: 19 (sd 10); T2DM: 18 (sd 11) ng/ml, P < 0·001). The overall prevalence of vitamin D deficiency was 55 % and was significantly higher among individuals with T2DM (63 %) followed by prediabetes (58 %) and NGT (51 %) (Pfor trend < 0·001). Women had 1·6 times the risk of vitamin D deficiency compared with men (unadjusted OR 1·6 (95 % CI 1·3, 2·0) and adjusted OR 1·6 (95 % CI 1·2, 1·9)). However, there was no increasing trend observed with increasing age. The prevalence of abdominal obesity (66 v. 49 %), generalised obesity (80 v. 64 %), the metabolic syndrome (45 v. 37 %) and insulin resistance (38 v. 27 %) was significantly higher in those with vitamin D deficiency compared with those without. This study shows that vitamin D deficiency is highly prevalent in this urban south Indian population and was higher among individuals with T2DM and prediabetes compared with those with NGT.

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Estrogen receptor α genetic variants and the risk of stroke in a South Indian population from Andhra Pradesh

Clinica Chimica Acta ◽

10.1016/j.cca.2010.08.001 ◽

2010 ◽

Vol 411 (21-22) ◽

pp. 1817-1821 ◽

Cited By ~ 15

Author(s):

Anjana Munshi ◽

Vandana Sharma ◽

Subhash Kaul ◽

Amal Al-Hazzani ◽

Ali A. Alshatwi ◽

...

Keyword(s):

Estrogen Receptor ◽

Genetic Variants ◽

Andhra Pradesh ◽

Indian Population ◽

Estrogen Receptor Α ◽

South Indian Population ◽

South Indian

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mRNA expression profile in peripheral blood mononuclear cells based on ADRB1 Ser49Gly and Arg389Gly polymorphisms in essential hypertension — a case-control pilot investigation in South Indian population

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2017-0882 ◽

2018 ◽

Vol 56 (8) ◽

pp. 1230-1237 ◽

Cited By ~ 1

Author(s):

Varsha Varakantham ◽

Ashok Kumar Kurakula Sailoo ◽

Balakrishna Nagalla ◽

Dinesh Kumar Bharatraj

Keyword(s):

Essential Hypertension ◽

Mrna Expression ◽

Genetic Variants ◽

Indian Population ◽

Mononuclear Cells ◽

Case Control ◽

South Indian Population ◽

Mrna Levels ◽

Peripheral Blood Mononuclear ◽

South Indian

AbstractBackground:β1-Adrenoreceptor (ADRB1) genetic polymorphisms are widely studied for susceptibility to many cardiovascular diseases such as essential hypertension. However, the mRNA expression ofADRB1is rarely studied.Methods:A case-control pilot study with 292 hypertensives and 324 controls was designed to evaluate the role of the Ser49Gly and Arg389Gly, which are commonly studied single nucleotide polymorphisms (SNP), in the mRNA levels ofADRB1, in conjunction with its genetic predisposition to essential hypertension.Results:Differential expression ofADRB1mRNA was seen between hypertensives and controls (p<0.01) based on genetic variants of Ser49Gly. Among hypertensive subjects, Ser49Ser and Gly49Gly were highly expressed in comparison to Ser49Gly (p<0.05 and p<0.01, respectively), whereas genetic variants of Arg389Gly did not demonstrate any such variations. We found no association between theADRB1SNPs viz., Ser49Gly and Arg389Gly and essential hypertension.Conclusions:The increased mRNA levels of Gly49Gly may indicate a plausible role in the interindividual variations in drug response. Further,ADRB1polymorphisms did not contribute to the genetic risk of essential hypertension. Studies with larger sample size are warranted to confirm these observations in the South Indian population.

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