Removal properties are researched and discussed of algae and nitrogen & phosphorus nutrients with periphyton by assembled substrate in lake-based eutrophic water body, including impacts on removal effects of several physical & chemical properties as water depth, water velocity, DO, temperature and PH. The results show that: the removal rate of NH4-N, other nitrogen nutrients, phosphorus nutrients and Chl-a were 96%, 40%, 80%, 65% respectively. With the growth of water velocity(from 0 to 200L/h ), the removal of Chl-a, TP, TDP increased to a certain extent(Chl-a: from 63.63% to 67.66%; TP:from 76.15% to 85.13%;TDP:from 80.10% to 88.44%),and that of TN,TDN and NO3-N decreased to different extents(TN: from 45.19% to 31.97%; TDN: from 42.81% to 28.77%; NO3-N: from 38.11% to –5.26%), but that of NH4-N and PO4-P altered little(NH4-N:from 96.80% to 95.94%;PO4-P:from 85.38% to 87.02%). It will provide reference for recovery and re-contribution of destroyed Eco-system, and a new thought for treatment in eutrophical lakes.