scholarly journals Effect of abiotic factors on the molluscicidal activity of oleoresin of Zingiber officinale against the snail Lymnaea acuminata

2010 ◽  
Vol 02 (10) ◽  
pp. 1148-1154
Author(s):  
Vijya Singh ◽  
Pradeep Kumar ◽  
V. K. Singh ◽  
D. K. Singh
2013 ◽  
Vol 55 (4) ◽  
pp. 251-259 ◽  
Author(s):  
Aparna Upadhyay ◽  
Vinay K. Singh ◽  
Dinesh K. Singh

SUMMARY The molluscicidal activity of the leaf powder of Moringa oleifera and lyophilized fruit powder of Momordica charantia against the snail Lymnaea acuminata was time and concentration dependent. M. oleifera leaf powder (96 h LC50: 197.59 ppm) was more toxic than M. charantia lyophilized fruit powder (96 h LC50: 318.29 ppm). The ethanolic extracts of M. oleifera leaf powder and Momordica charantia lyophilized fruit powder were more toxic than other organic solvent extracts. The 96 h LC50 of the column purified fraction of M. oleifera leaf powder was 22.52 ppm, while that of M. charantia lyophilized fruit powder was 6.21 ppm. Column, thin layer and high performance liquid chromatography analysis show that the active molluscicidal components in M. oleifera leaf powder and lyophilized fruit of M. charantia are benzylamine (96 h LC50: 2.3 ppm) and momordicine (96 h LC50: 1.2 ppm), respectively. Benzylamine and momordicine significantly inhibited, in vivo and in vitro, the acetylcholinesterase (AChE), acid and alkaline phosphatase (ACP/ALP) activities in the nervous tissues of L. acuminata. Inhibition of AChE, ACP and ALP activity in the nervous tissues of L. acuminata by benzylamine and momordicine may be responsible for the molluscicidal activity of M. oleifera and M. charantia fruits, respectively.


2021 ◽  
Vol 40 (2) ◽  
pp. 40-50
Author(s):  
Hasnun Nita Ismail ◽  
Nadia Nisha Musa

The study was conducted to assess the effect of Zingiber officinale and Carica papaya extract on the egg hatchability of the golden apple snail, Pomacea canaliculata. This is a freshwater snail that is considered a pest in agriculture as well as natural ecosystems. In combating the population of the aforementioned pest, this study evaluates the use of plant extracts such as biomolluscicides to control the production of new hatchlings from the clusters of eggs. The egg clusters were exposed to different concentrations of Z. officinale ethanolic extract and C. papaya methanolic extracts (25 ppm, 50 ppm, 100 ppm) using the direct single spraying method. Observation was made for a maximum of 30 days. The time taken for the eggs to hatch and the number of eggs successfully hatched were measured to reflect the potential of both extracts in affecting egg hatchability. The finding shows that the eggs hatch later when exposed to increasing concentrations (25 to 100 ppm) of Z. officinale extract. The number of eggs successfully hatched was significantly decreased from 86% to 9% after exposure to 25 ppm and above. Meanwhile, the extract of C. papaya did not show any significant effect on both parameters for egg hatchability of P. canaliculata, regardless of the increase in concentration from 25 to 100 ppm. Our findings reveal that the extract of Z. officinale contains higher molluscicidal activity than the extract of C. papaya. Therefore, it has the potential to be commercialized as a biomolluscicide to control the population of golden apple snails specifically at the egg stage.


Author(s):  
R. N. SINGH ◽  
PRADEEP KUMAR ◽  
NAVNEET KUMAR ◽  
D. K. SINGH

Objective: The objective of the present study is the evaluation of the effect of the sublethal (40% and 60% of 48h LC50) binary combination (1:5 ratios) of molluscicides deltamethrin+MGK-264 on the endogenous levels of protein, amino acid and nucleic acid in different tissues of snail Lymnaea acuminata. Methods: The snails were treated with 1:5 mixtures of sub-lethal concentration of (40% and 60% of 48h LC50) deltamethrin+MGK-264 on the protein, amino acid and nucleic acid levels in gonadal, nervous and foot tissue of L. acuminata. In order to study the effect of withdrawal from treatment, the snails were first exposed to the above concentrations for 96h, after which they were transferred to freshwater. Water was changed every 24h for the next seven days, after which different biochemical parameters were estimated. Results: There was a significant change in the levels of protein (sublethal concentration of 60% of 48h LC50 after 96h) gonadal, nervous and foot tissues are 48.1, 12.1 and 14.5%, respectively, amino acid are 273, 234 and 252%, respectively, DNA are 25.1, 38.9 and 42.1%, respectively and RNA are 12.2, 30.7 and 30.5%, respectively. These changes were time and concentration-dependent. In the withdrawal experiment, the snails were treated for 96h to transfer in freshwater for 7 d, which caused significant recovery in all the biochemical parameters. Conclusion: The present study concluded that the high molluscicidal activity of deltamethrin+MGK-264 simultaneous decrease in the levels of proteins, DNA, RNA and increase in the level of amino acids.


2009 ◽  
Vol 8 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Harsh Vardhan Jigyasu ◽  
Vinay Kumar Singh

Lymnaea acuminata breeds round the year. The effect of pH, temperature, dissolved oxygen, carbon dioxide, light/dark period and clean/polluted water on the fecundity, hatchability and survival of young snails of L. acuminata were studied. It was observed that these environmental variant abiotic factors caused a significant variation in fecundity, hatchability and survival of young snails. Maximum reproduction of this snail was observed in the months of March to May. A significant positive correlation (p<0.05) between D.O. (3.1–7.7 ppm)/pH (7.01–8.96) of water with fecundity (6.0–196.33/20 snails), hatchability (54.69–96.91%) and survival (61.3–95.86%) of young snails was observed for each month and each interval of 24–72 h. In contrast, a significant negative correlation between dissolved CO2 (4.6–16.6 ppm)/temperature (16–37°C) of water was noted with fecundity, hatchability and survival of snails. Percent hatchability in the eggs in different regimens of water was between 96.91–54.69%. The hatching period was prolonged (2–14 days) in snail exposed to different groups of water compared to the control group (2–9 d). This study conclusively shows that variant abiotic factors in different months of the year can significantly alter the reproductive ability and development process in the snail Lymnaea acuminata.


2019 ◽  
Vol 22 (2) ◽  
pp. 526-534 ◽  
Author(s):  
Paulo Roberto Barros Gomes ◽  
Romer Pessôa Fernandes ◽  
Jonas Batista Reis ◽  
Maria Alves Fontenele ◽  
Adriana Crispim de Freitas ◽  
...  

2011 ◽  
Vol 53 (5) ◽  
pp. 265-270 ◽  
Author(s):  
Saroj Chauhan ◽  
Ajay Singh

The present work was carried out to evaluate the molluscicidal activity of active ingredient Taraxerol with the acetone extract of Euphorbia tirucalli against Lymnaea acuminata snail. The (fecundity, hatchability and survivability) of snail L. acuminata exposed to this extract was studied. The effects of the tested extracts on life-history traits of harmful snail L. acuminata have also been evaluated, and this study also expounds the inhibitory effects of these extracts singly as well as in binary combination (1:1 ratio). It concluded that these herbal products act as a potential source of molluscicides, and that they would also have the advantage of easy availability, low cost, biodegradability and greater acceptance amongst users than synthetic pesticide.


2012 ◽  
Vol 54 (3) ◽  
pp. 135-140 ◽  
Author(s):  
Kanchan Lata Singh ◽  
D.K. Singh ◽  
Vinay Kumar Singh

The molluscicidal activity of Bauhinia variegata leaf and Mimusops elengi bark was studied against vector snail Lymnaea acuminata. The toxicity of both plants was time and concentration-dependent. Among organic extracts, ethanol extracts of both plants were more toxic. Toxicity of B. variegata leaf ethanolic extract (96h LC50- 14.4 mg/L) was more pronounced than M. elengi bark ethanolic extract (96h LC50-15.0 mg/L). The 24h LC50 of column purified fraction of B. variegata and M. elengi bark were 20.3 mg/L and 18.3 mg/L, respectively. Saponin and quercetin were characterized and identified as active molluscicidal component. Co-migration of saponin (Rf 0.48) and quercetin (Rf 0.52) with column purified bark of M. elengi and leaf of B. variegata on thin layer chromatography demonstrate same Rf value i.e. 0.48 and 0.52, respectively. The present study clearly indicates the possibility of using M. elengi and/or B. variegata as potent molluscicide.


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