scholarly journals Effect of Direct-Fed Microbial Supplementation on Pathogenic <i>Escherichia coli</i> Fecal Shedding, Live Performance, and Carcass Characteristics in Feedlot Steers

2020 ◽  
Vol 10 (04) ◽  
pp. 683-705
Author(s):  
Alex Thompson ◽  
M. Alexandra Calle ◽  
Flavio R. Ribeiro ◽  
Ansen R. Pond ◽  
Wanda M. Kreikemeier ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carcass Characteristics ◽  
Live Performance ◽  
Fecal Shedding ◽  
Feedlot Steers

Comparative Effect of Direct-Fed Microbials on Fecal Shedding of Escherichia coli O157:H7 and Salmonella in Naturally Infected Feedlot Cattle

2008 ◽  
Vol 71 (3) ◽  
pp. 539-544 ◽  
Author(s):  
EBOT S. TABE ◽  
JAMES OLOYA ◽  
DAWN K. DOETKOTT ◽  
MARC L. BAUER ◽  
PENELOPE S. GIBBS ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Control Group ◽  
Escherichia Coli O157 ◽  
Comparative Effect ◽  
Laboratory Methods ◽  
Fecal Shedding ◽  
E Coli ◽  
Feedlot Steers ◽  
And Control

The effect of direct-fed microbials (DFM) on fecal shedding of Escherichia coli O157:H7 and Salmonella in naturally infected feedlot cattle was evaluated in a clinical trial involving 138 feedlot steers. Following standard laboratory methods, fecal samples collected from steers were evaluated for change in the detectable levels of E. coli O157:H7 and Salmonella shed in feces after DFM treatment. Sampling of steers was carried out every 3 weeks for 84 days. A significant reduction (32%) in fecal shedding of E. coli O157:H7 (P &lt; 0.001), but not Salmonella (P = 0.24), was observed among the treatment steers compared with the control group during finishing. The probability of recovery of E. coli O157:H7 from the feces of treated and control steers was 34.0 and 66.0%, respectively. Steers placed on DFM supplement were almost three times less likely to shed E. coli O157:H7 (odds ratio, 0.36; 95% confidence interval, 0.25 to 0.53; P &lt; 0.001) in their feces as opposed to their control counterparts. The probability of recovery of Salmonella from the feces of the control (14.0%) and the treated (11.3%) steers was similar. However, the DFM significantly reduced probability of new infections with Salmonella among DFM-treated cattle compared with controls (nontreated ones). It appears that DFM as applied in our study are capable of significantly reducing fecal shedding of E. coli O157:H7 in naturally infected cattle but not Salmonella. The factors responsible for the observed difference in the effects of DFM on E. coli O157:H7 and Salmonella warrants further investigation.

Fecal Shedding of Foodborne Pathogens by Florida-Born Heifers and Steers in U.S. Beef Production Segments†

2008 ◽  
Vol 71 (4) ◽  
pp. 807-810 ◽  
Author(s):  
D. G. RILEY ◽  
G. H. LONERAGAN ◽  
W. A. PHILLIPS ◽  
J. T. GRAY ◽  
P. J. FEDORKA-CRAY
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Odds Ratio ◽  
Local Development ◽  
Development Program ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
E Coli ◽  
Feedlot Steers ◽  
Secondary Objective

The objective in this study was to assess breed effects in fecal prevalence of Escherichia coli O157:H7 in heifers on a development program in Florida and in their steer half siblings in stocker and feedlot phases in Oklahoma. A secondary objective was to characterize fecal shedding of Campylobacter and Salmonella in subsets of the same samples. After weaning, heifers (n = 501; purebreds and F1 crosses of Angus, Brahman, and Romosinuano) were preconditioned and placed in a local development program. Steers (n = 481) were transported to Oklahoma, where they grazed wheat for 6 months and then were placed in feedlot pens. Fecal samples were obtained at least every 28 days for 12 months on most animals. None of the 10,982 samples tested positive for E. coli O157:H7. Overall fecal prevalences of Campylobacter and Salmonella in heifers were 1.7 and 0.04%, respectively. Corresponding overall prevalences in steer samples were 27.2 and 0.6%. Campylobacter isolates were mostly C. jejuni and were tetracycline resistant. Eight Salmonella isolates were Salmonella Typhimurium that were either quad or penta resistant, most often to ampicillin, chloramphenicol, sulfamexathole, and tetracycline. Feedlot steers had greater odds of positive detection of Campylobacter (odds ratio, 8.5; confidence interval, 3.7, 19.5) than when grazing winter wheat. No breed effect was detected for fecal prevalence of these pathogens.

Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep

2009 ◽  
Vol 89 (2) ◽  
pp. 285-293 ◽  
Author(s):  
S J Bach ◽  
R P Johnson ◽  
K. Stanford ◽  
T A McAllister
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Total Coliforms ◽  
E Coli ◽  
Oral Inoculation ◽  
Experimental Treatments ◽  
And Control

Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages (phage), and control (CON). Oral inoculation of the rams with 109 CFU of a mixture of four nalidixic acid-resistant strains of E. coli O157:H7 was performed on day 0. A mixture of 1010 PFU of bacteriophages P5, P8 and P11 was administered on days -2, -1, 0, 6 and 7. Fecal samples collected on 14 occasions over 21 d were analyzed for E. coli O157:H7, total E. coli, total coliforms and bacteriophages. Sheep in treatment O157+phage shed fewer (P < 0.05) E. coli O157:H7 than did sheep in treatment O157. Populations of total coliforms and total E. coli were similar (P < 0.05) among treatments, implying that bacteriophage lysis of non-target E. coli and coliform bacteria in the gastrointestinal tract did not occur. Bacteriophage numbers declined rapidly over 21 d, which likely reduced the chance of collision between bacteria and bacteriophage. Oral administration of bacteriophages reduced shedding of E. coli O157:H7 by sheep, but a delivery system that would protect bacteriophages during passage through the intestine may increase the effectiveness of this strategy as well as allow phage to be administered in the feed.Key words: Escherichia coli O157:H7, bacteriophage, sheep, environment, coliforms

scholarly journals Effects of voluntary removal of ractopamine hydrochloride (Optaflexx) on live performance and carcass characteristics of beef steers

2021 ◽  
Author(s):  
P J Rincker ◽  
J B Allen ◽  
M Edmonds ◽  
M S Brown ◽  
J C Kube
Keyword(s):  
Dry Matter ◽  
Carcass Characteristics ◽  
Carcass Weight ◽  
Dry Matter Intake ◽  
Beef Steers ◽  
Live Performance ◽  
Meet Residue ◽  
Ractopamine Hydrochloride ◽  
The Impact ◽  
Linear Manner

Abstract There is a lack of consistency across the globe in how countries establish tissue ractopamine residue limits and which residue limits are applied to various tissues, particularly for edible noncarcass tissues. Therefore, some US beef slaughter organizations have recommended a 48-h voluntary removal of ractopamine before slaughter in order to meet residue requirements of specific export countries and maintain international trade. Our objective was to assess the impact of voluntary removal of ractopamine hydrochloride (Optaflexx®; Elanco, Greenfield, IN) up to 8 d before slaughter on growth performance and carcass characteristics. Crossbred beef steers (60 pens of 10 animals/pen) with an initial shrunk body weight (BW) of 611.8 ± 10 kg SEM were fed one of six treatments over 42 d. Treatments included a control that did not receive ractopamine, on-label use of ractopamine (0-d withdrawal), and 2, 4, 6, or 8 d of voluntary removal of ractopamine from feed before slaughter. The start of ractopamine feeding (30.1 mg/kg of diet dry matter for 32 d) was staggered so that blocks could be slaughtered on the same day. Dry matter intake was decreased by 0.5 kg/d when ractopamine was fed with a 0-d withdrawal (P = 0.04) compared to the control, but was not altered (P = 0.56) as the duration of ractopamine removal increased from 0 to 8 d. Final BW, total BW gain, and average daily BW gain were increased by feeding ractopamine with a 0-d withdrawal (P = 0.09) compared to the control, but these variables decreased in a linear manner (P = 0.10) as the duration of removal increased from 0 to 8 d. Gain efficiency was improved by 15% (P &lt; 0.01) by feeding ractopamine with a 0-d withdrawal compared to the control, and gain efficiency decreased linearly (P = 0.06) as the duration of ractopamine removal increased. Approximately 2/3 of the increase in gain efficiency remained after 8 d of removal. Hot carcass weight was increased by 6 kg (P = 0.02) by feeding ractopamine with a 0-d withdrawal compared to the control. Measured carcass characteristics were not altered by ractopamine feeding or by removal before slaughter (P ≥ 0.24). The consequences of voluntary removal of ractopamine up to 8 d before slaughter were a linear decrease in live BW gain (0.64 kg/d), poorer gain efficiency, and numerically lighter carcass weight.

EFFECTS OF DIETARY COMPOSITION ON THE RELATIVE PERFORMANCE AND CARCASS CHARACTERISTICS OF MALE AND FEMALE PIGS FED FROM 25 KG TO 92 KG LIVEWEIGHT

1976 ◽  
Vol 56 (3) ◽  
pp. 439-450
Author(s):  
A. G. CASTELL ◽  
D. T. SPURR
Keyword(s):  
Carcass Characteristics ◽  
Sole Source ◽  
Relative Performance ◽  
Restricted Feeding ◽  
Finishing Pigs ◽  
Live Performance ◽  
Feed Conversion ◽  
Protein Levels ◽  
Satisfactory Performance ◽  
Field Peas

One hundred and twenty-four feeder pigs, representing two breed groups (Hampshire × Yorkshire and Yorkshire) were used in three experiments to compare the live performance and carcass characteristics of boars, barrows and gilts fed, from 25 to 92 kg, a 16% protein, barley–soybean meal diet or diets based on a utility grade, soft wheat (Triticum aestivum cv. Pitic 62) with 0, 7½ and 15% field peas (Pisum sativum cv. Century) to provide total protein levels of 13, 14 or 15%. Under conditions of adequate nutrition, boars were superior to barrows in efficiency of feed conversion and estimates of carcass leanness, while gilts were intermediate in feed conversion and equivalent to boars in carcass indices. The amount and composition of the diet consumed had a greater effect upon the relative performance of boars and, to a lesser extent, of gilts than was observed with barrows. In addition, the dressing percentage was consistently lower with boars than with barrows or gilts. Sexual odor or taint was not noticeably present in the carcasses at the packing plant, although there was a tendency for meat from boars on restricted feeding to have a higher moisture and darker lean than that present in carcasses from barrows and gilts. The results also suggested that satisfactory performance of growing–finishing pigs could be achieved with diets based on Pitic 62 wheat using field peas as the sole source of supplementary protein.

Evaluation of Commercial β-Agonists, Dietary Protein, and Shade on Fecal Shedding of Escherichia coli O157:H7 from Feedlot Cattle

2017 ◽  
Vol 14 (11) ◽  
pp. 649-655 ◽  
Author(s):  
James E. Wells ◽  
Elaine D. Berry ◽  
Minseok Kim ◽  
Steven D. Shackelford ◽  
Kristin E. Hales
Keyword(s):  
Escherichia Coli ◽  
Dietary Protein ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding
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