HTRF-based high-throughput PGE2 release prohibition model and application in discovering traditional Chinese medicine active ingredients

2016 ◽  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Throughput ◽  
Active Ingredients ◽  
Pge2 Release

The Application of High Throughput Microarrays in the Screening Active Ingredients of Traditional Chinese Medicine

2014 ◽  
Vol 998-999 ◽  
pp. 346-349
Author(s):  
Qing Shan Liu ◽  
Wei Wei Zhang ◽  
Xu Li ◽  
Shu Juan Zhuang ◽  
Xiao Ying Yin
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Throughput ◽  
Detection Method ◽  
Active Ingredients ◽  
Chinese Medicines ◽  
Accurate Detection ◽  
And Control ◽  
Research Tools

The accurate detection of traditional Chinese medicine is significant for diagnosis, treatment and control for disease. There is an urgent need for the development of a rapid, simple, and accurate detection method. The high throughput microarray is recommended for use in all researches including those involving rare samples and expensive reagents. Due to the complexity of Chinese medicines interference and multi-target, multi-component, the advantages that sensitivity, reproducibility, and specificity of high-throughput microarrays make it become one of the effective research tools.

scholarly journals Stability of Active Ingredients of Traditional Chinese Medicine (TCM)

2009 ◽  
Vol 4 (12) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Wang Meng ◽  
Ren Xiaoliang ◽  
Gao Xiumei ◽  
Franco Francesco Vincieri ◽  
Anna Rita Bilia
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Animal Origin ◽  
Active Ingredients ◽  
Rational Development ◽  
Plant Constituents ◽  
Crude Drugs ◽  
The Stability ◽  
First Time

Studies on stability of active ingredients are fundamental and critical for the rational development of Traditional Chinese Medicine (TCM) in view of its modernization and worldwide use. The stability of both active and marker constituents of plants used in TCM is reviewed for the first time. More than 100 papers, mostly written in Chinese, have been reviewed. Studies concerning plant constituents were analyzed according to their chemical classification of active ingredients. In addition, several crude drugs of animal origin are also reported. Stability of active ingredients is summarized during extraction and/or storage of the herbal drug preparations, and under stress conditions (pH, temperature, solvents, light, and humidity) and in the presence of preservatives, antioxidants, and metals.

Astragaloside IV Inhibits Membrane Ca2+ Current but Enhances Sarcoplasmic Reticulum Ca2+ Release

2017 ◽  
Vol 45 (04) ◽  
pp. 863-877 ◽  
Author(s):  
Mei-Mi Zhao ◽  
Wen-Wen Lian ◽  
Zhuo Li ◽  
Dong-Xue Shao ◽  
Si-Chong Chen ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Sarcoplasmic Reticulum ◽  
Traditional Chinese Medicine ◽  
Cardiac Myocytes ◽  
Cardiac Disease ◽  
H9c2 Cells ◽  
Inhibitory Effects ◽  
Astragaloside Iv ◽  
Active Ingredients ◽  
Dependent Inactivation

Astragaloside IV (AS-IV) is one of the active ingredients in Astragalus membrananceus (Huangqi), a traditional Chinese medicine. The present study investigated the effects of AS-IV on Ca[Formula: see text] handling in cardiac myocytes to elucidate its possible mechanism in the treatment of cardiac disease. The results showed that AS-IV at 1 and 10[Formula: see text][Formula: see text]M reduced KCl-induced [Ca[Formula: see text]]i increase ([Formula: see text] from 1.33[Formula: see text][Formula: see text][Formula: see text]0.04 (control, [Formula: see text] 28) to 1.22[Formula: see text][Formula: see text][Formula: see text]0.02 ([Formula: see text], [Formula: see text] 29) and 1.22[Formula: see text][Formula: see text][Formula: see text]0.02 ([Formula: see text] 0.01, [Formula: see text]), but it enhanced Ca[Formula: see text] release from SR ([Formula: see text] from 1.04[Formula: see text][Formula: see text][Formula: see text]0.01 (control, [Formula: see text]) to 1.44[Formula: see text][Formula: see text][Formula: see text]0.03 ([Formula: see text], [Formula: see text]) and 1.60[Formula: see text][Formula: see text][Formula: see text]0.04 ([Formula: see text] 0.01, [Formula: see text]0), in H9c2 cells. Similar results were obtained in native cardiomyocytes. AS-IV at 1 and 10[Formula: see text][Formula: see text]M inhibited L-type Ca[Formula: see text] current ([Formula: see text] from [Formula: see text]4.42[Formula: see text][Formula: see text][Formula: see text]0.58 pA/pF of control to [Formula: see text]2.25[Formula: see text][Formula: see text][Formula: see text]0.12 pA/pF ([Formula: see text] 0.01, [Formula: see text] 5) and [Formula: see text]1.78[Formula: see text][Formula: see text][Formula: see text]0.28 pA/pF ([Formula: see text] 0.01, [Formula: see text] 5) respectively, when the interference of [Ca[Formula: see text]]i was eliminated due to the depletion of SR Ca[Formula: see text] store by thapsigargin, an inhibitor of Ca[Formula: see text] ATPase. Moreover, when BAPTA, a rapid Ca[Formula: see text] chelator, was used, CDI (Ca[Formula: see text]-dependent inactivation) of [Formula: see text] was eliminated, and the inhibitory effects of AS-IV on ICaL were significantly reduced at the same time. These results suggest that AS-IV affects Ca[Formula: see text] homeostasis through two opposite pathways: inhibition of Ca[Formula: see text] influx through L-type Ca[Formula: see text] channel, and promotion of Ca[Formula: see text] release from SR.

Molecularly Imprinted Solid-Phase Extraction: Extraction of Traditional Chinese Medicine Active Ingredients

2014 ◽  
Vol 665 ◽  
pp. 311-314
Author(s):  
Qing Shan Liu ◽  
Xu Li ◽  
Shu Juan Zhuang ◽  
Wei Wei Zhang ◽  
Xiao Ying Yin ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Active Ingredients ◽  
Molecularly Imprinted ◽  
Extraction And Separation ◽  
Synthetic Materials ◽  
Molecular Imprinting Technology

Molecular imprinting technology (MIT) is a developing technique with high recognition which is just like the recognition between enzymes and antibodies in the organism. Molecularly imprinted polymers (MIPs), synthetic materials obtained using the imprinting technology, have played a huge advantage and been used in many fields. Especially, MIPs have been applied to the extraction and separation of analytes as the selective adsorbent of solid-phase extraction (SPE), which is known as molecularly imprinted polymer solid-phase extraction (MISPE) in recent years. In the present review, the methodology of MIPs preparation and evaluation are explained. Moreover, recent great developments of SPE and MISPE are discussed, and the potential application of MISPE in extraction of traditional Chinese medicine (TCM) active ingredients are also presented briefly.

scholarly journals A Network Pharmacology-Based Study on Active Ingredients of Corydalis Rhizoma on Coronary Heart Disease

2020 ◽  
Author(s):  
Ying Li ◽  
Guhang Wei ◽  
Zhenkun Zhuang ◽  
Mingtai Chen ◽  
Changjian Yuan ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Signaling Pathway ◽  
Network Pharmacology ◽  
Active Ingredients ◽  
Active Components

Abstract BackgroundCorydalis Rhizoma(CR) showed a high efficacy for coronary heart disease (CHD). However, the interaction between the active ingredients of CR and the targets of CHD has not been unequivocally explained in previous researches. To study the active components and potential targets of Corydalis Rhizoma and to determine the mechanism underlying the exact effect of Corydalis Rhizoma on coronary heart disease, a method of network pharmacology was used.Materials and MethodsThe active components of CR and targets corresponding to each component were scanned out from Traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP), and target genes of CHD were searched on GeneCards database and Online Mendelian Inheritance in Man(OMIM) database. The active components and common targets of CR and CHD were used to build the “CR-CHD” network through Cytoscape (version 3.2.1) software as well as protein-protein interaction(PPI) network on String database. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis was executed by clusterProfiler(version 3.8) and DOSE(version 3.6) package on R platform.Results49 active ingredients and 394 relevant targets of CR and the 7173 CHD-related genes were retrieved. 40 common genes were selected for subsequent analysis. Crucial biological processes and pathways were obtained and analyzed, including DNA-binding transcription activator activity, RNA polymerase II-specific, RNA polymerase II transcription factor binding, kinase regulator activity, ubiquitin-like protein ligase binding, fluid shear stress and atherosclerosis, TNF signaling pathway, apoptosis, MAPK signaling pathway and PI3K-Akt signaling pathway.ConclusionsOverall, CR could alleviate CHD through the mechanisms predicted by network pharmacology, laying the foundation for future development of new drugs from traditional Chinese medicine on CHD.

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