scholarly journals Evaluation of the Efficacy of 2% Chlorhexidine in Combination with Passive Ultrasonic Irrigation on Enterococcus faecalis Biofilm

2020 ◽  
Vol 21 (2) ◽  
pp. 140-146
Author(s):  
Bertan Kesim ◽  
Seda Tezcan Ülger ◽  
Hüseyin Sinan Topçuoğlu ◽  
Gönül Aslan ◽  
Nurbanu Kurnaz ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation

scholarly journals Effect of sodium hypochlorite solution and gel with/without passive ultrasonic irrigation on Enterococcus faecalis, Escherichia coli and their endotoxins

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 642
Author(s):  
Amjad Abu Hasna ◽  
Laiana Pereira Da Silva ◽  
Fernanda Carvalho Pelegrini ◽  
Cláudia Luísa Ribeiro Ferreira ◽  
Luciane Dias de Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Ethylenediaminetetraacetic Acid ◽  
Lipoteichoic Acid ◽  
Sodium Hypochlorite Solution ◽  
Passive Ultrasonic Irrigation ◽  
E Coli ◽  
Significant Difference ◽  
Ultrasonic Irrigation

Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The purpose of this study was to evaluate the effect of NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on Enterococcus faecalis, Escherichia coli, and their endotoxins, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Methods: 40 human lower premolars were contaminated with E. coli (ATCC 25922) for 28 days and E. faecalis (ATCC 29212) for 21 days. Specimens were randomly divided into four groups: (1) 2.5% NaOCl irrigating the canals without PUI activation; (2) 2.5% NaOCl with PUI; (3) 3% NaOCl gel irrigating the canals without PUI; and (4) 3% NaOCl gel with PUI. 40 mL of irrigant was used for each group. PUI activation was carried out using E1-Irrisonic stainless-steel tip at 10% frequency. After treatment, all specimens were filled with 3mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3min and then washed with nonpyrogenic saline solution. Three samples were collected from the canals: S1, at baseline to confirm biofilm formation; S2 after treatment; and S3 after EDTA. Samples were assessed for E. coli and E. faecalis colony forming units, and LPS and LTA were assessed using chromogenic kinetic LAL assay and ELISA, respectively. Data were analyzed by Kruskal-Wallis, Friedmann and Dunn tests with α≤0.05. Results: All groups were effective in reducing the microbial load of E. coli and E. faecalis after treatment without a significant difference among the groups. NaOCl and NaOCl gel groups had no significant difference in reducing LPS and LTA. Statistically increased reduction was seen for NaOCL + PUI and NaOCl gel + PUI compared for groups without PUI. Conclusions: NaOCl gel has the same antimicrobial action of NaOCl solution and can partially detoxify endotoxins. PUI improves NaOCl (gel or solution) action over E. faecalis and E. coli and their endotoxins.

scholarly journals Effect of Passive Ultrasonic Irrigation on Enterococcus faecalis from Root Canals: An Ex Vivo Study

2015 ◽  
Vol 26 (4) ◽  
pp. 342-346 ◽  
Author(s):  
Juliane Maria Guerreiro-Tanomaru ◽  
Gisselle Moraima Chávez-Andrade ◽  
Norberto Batista de Faria-Júnior ◽  
Evandro Watanabe ◽  
Mário Tanomaru-Filho
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Saline Solution ◽  
Ex Vivo ◽  
Canal System ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Significant Difference ◽  
Ultrasonic Irrigation ◽  
Root Canal System

<p>Endodontic irrigation aims to clean and disinfect the root canal system. Passive ultrasonic irrigation (PUI) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. The aim of this study was to evaluate, ex vivo, the effectiveness of PUI in eliminating <italic>Enterococcus faecalis</italic> from root canals. Seventy-five extracted human single-root teeth were used. After root canal preparation, specimens were inoculated with <italic>E. faecalis</italic> and incubated at 37 °C for 21 days. Specimens were distributed into five groups (n=15), according to the irrigation method: PUI + saline solution (PUI/SS); PUI + 1% NaOCl (PUI/NaOCl); conventional needle irrigation (CNI) + saline solution (CNI/SS); CNI + 1% NaOCl (CNI/NaOCl); No irrigation (control). Microbiological samples were collected at three time points: initial (21 days after inoculation), post-irrigation (immediately after irrigation), and final (7 days after irrigation). Data were obtained in CFU mL<sup>-1</sup> and subjected to analysis by ANOVA and Tukey's tests at 5% significance level. The post-irrigation samples did not demonstrate statistical difference between PUI/SS and CNI/SS nor between PUI/NaOCl and CNI/NaOCl (p>0.05), but PUI/NaOCl and CNI/NaOCl had lower CFU mL<sup>-1</sup> number than the other groups (p>0.05). Statistically significant difference was observed between the initial and post-irrigation samples and between the post-irrigation and final samples (p<0.05) in all groups, except in the control. The final samples of all groups presented bacterial counts similar to the initial samples. PUI or CNI with 1% NaOCl contribute to disinfection, but are unable to eradicate <italic>E. faecalis</italic> from the root canal system.</p>

The Influence of Passive Ultrasonic Irrigation Associated with Different Irrigating Solutions on Radicular Dentin Microhardness

2020 ◽  
Vol 04 (04) ◽  
Author(s):  
Sandra Pillon Nogueira ◽  
Carlos Eduardo da Silveira Bueno ◽  
Alexandre Sigrist De Martin ◽  
Rina Andrea Pelegrine ◽  
Carlos Eduardo Fontana ◽  
...  
Keyword(s):  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation

scholarly journals Outcomes of Endodontic Therapy Comparing Conventional Sodium Hypochlorite Irrigation with Passive Ultrasonic Irrigation Using Sodium Hypochlorite and Ethylenediaminetetraacetate. A Retrospective Analysis

2016 ◽  
Vol 10 (1) ◽  
pp. 375-381 ◽  
Author(s):  
Moritz Hertel ◽  
Katja Sommer ◽  
Eckehard Kostka ◽  
Sandra Maria Imiolczyk ◽  
Husam Ballout ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Clinical Symptoms ◽  
Treatment Success ◽  
Success Rates ◽  
Chi Square ◽  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation ◽  
Endodontic Irrigation ◽  
Group 2

The aim of the present study was to investigate the clinical outcomes of two different standardized endodontic irrigation protocols. It was assumed that the additional use of ethylenediaminetetraacetate (EDTA) and passive ultrasonic irrigation (PUI) would result in an increased rate of absence of symptoms and remission based on the periapical index (PAI) compared to passive irrigation using only sodium hypochlorite (NaOCl). Data and radiographs from 199 teeth retrieved from the institutional endodontic database were analyzed retrospectively. In 106 teeth irrigation was performed using only NaOCl (protocol 1). Ninety-three teeth were irrigated using NaOCl and EDTA (protocol 2). Chlorhexidine (CHX) was additionally used in revision treatments in both groups. All irrigants in group 2 were activated by PUI. Mean follow-up periods were: protocol 1 = 9.2 ± 4.4 and protocol 2 = 6.6 ± 2.5 months (p < 0.0001 (chi-square test). The frequencies of the PAImasterpoint and PAIfollow-up scores did not differ significantly between teeth, which received either protocol 1 or 2 (p = 0.555 and 0.138). Statistical analysis revealed no significant association between treatment success (absence of clinical symptoms and PAIfollow-up = I or PAImasterpoint > PAIfollow-up > I) and the applied protocol (success rates: protocol 1 = 72.6% vs. protocol 2 = 82.8%; p = 0.203). Furthermore, the frequency of extractions did not differ significantly between the two protocols (p = 0.102). No association was found between follow-up time and treatment success (p = 0.888). The hypothesis was not confirmed. Even though the obtained success rate was higher after supplementing the irrigation protocol with EDTA and PUI, no significance was recorded. Hence, protocol 2 was not superior to protocol 1 regarding therapy success, at least within the limited follow-up period. It may be cautiously concluded that sufficient mechanical debridement combined with passive NaOCl irrigation results in comparably high success rates compared to EDTA and PUI.

Evaluation of the Efficacy of EASYDO ACTIVATOR Irrigation Technique in Intracanal Smear Layer and Debris Removal from Mature Permanent Teeth

2021 ◽  
Author(s):  
Shao-Hui Zhang ◽  
Zheng-Rong Gao ◽  
Dusenge Marie Aimee ◽  
Yao Feng ◽  
Jing Hu ◽  
...  
Keyword(s):  
Smear Layer ◽  
Permanent Teeth ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Effective Removal ◽  
Apical Direction ◽  
Debris Removal ◽  
Ultrasonic Irrigation ◽  
Group 3 ◽  
Group 2

Abstract EASYDO ACTIVATOR (EA) is a continuously vibrational device for root canals irrigation, but its cleaning effectiveness has not been evaluated by any published reports. We were aiming to evaluate whether EA results in a greater intracanal smear layer and debris removal than conventional needle irrigation (NI) and passive ultrasonic irrigation (PUI). Twenty-seven single-rooted teeth were used. Canals were sized to R30 and irrigated with 3% sodium hypochlorite. Species were divided into three groups: Group 1: NI; Group 2: PUI; Group 3: EA. Canal walls were subjected to scanning electron microscopy. NI- and PUI-group canal cleanliness decreased from the coronal to apical direction (P < 0.05), except for EA group in the apical third. PUI removed more smear layer from the coronal and middle thirds than EA and NI (P < 0.05). PUI and EA were superior to NI regarding debris removal (P < 0.05). The smear layer and debris from the coronal and middle thirds were effectively removed with EA and PUI. More effective removal occurred from the apical third for EA compared with PUI (P < 0.05). Both methods removed smear layer and debris better than NI, providing a theoretical basis for the clinical application of EA.

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