scholarly journals Passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against Enterococcus faecalis

2014 ◽  
Vol 56 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Yoshimi Kobayashi ◽  
Makoto Hayashi ◽  
Fumihiko Yoshino ◽  
Muneaki Tamura ◽  
Ayaka Yoshida ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radical ◽  
Enterococcus Faecalis ◽  
Bactericidal Effect ◽  
Passive Ultrasonic Irrigation ◽  
Low Concentration ◽  
Ultrasonic Irrigation ◽  
Radical Generation

scholarly journals Bactericidal Effect of Photolysis of H2O2 in Combination with Sonolysis of Water via Hydroxyl Radical Generation

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132445 ◽  
Author(s):  
Hong Sheng ◽  
Keisuke Nakamura ◽  
Taro Kanno ◽  
Keiichi Sasaki ◽  
Yoshimi Niwano
Keyword(s):  
Hydroxyl Radical ◽  
Bactericidal Effect ◽  
Radical Generation

scholarly journals Evaluation of the Efficacy of 2% Chlorhexidine in Combination with Passive Ultrasonic Irrigation on Enterococcus faecalis Biofilm

2020 ◽  
Vol 21 (2) ◽  
pp. 140-146
Author(s):  
Bertan Kesim ◽  
Seda Tezcan Ülger ◽  
Hüseyin Sinan Topçuoğlu ◽  
Gönül Aslan ◽  
Nurbanu Kurnaz ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation

Mechanism of Cu removal during CMP in H2O2-glycine based slurries

1999 ◽  
Vol 566 ◽  
Author(s):  
M. Hariharaputhiran ◽  
S. Ramarajan ◽  
Y Li ◽  
S.V. Babu ◽  
S.V. Babu
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radical ◽  
Dissolution Rate ◽  
Film Formation ◽  
Ex Situ ◽  
Copper Dissolution ◽  
Radical Generation ◽  
Cu Cmp ◽  
Cu Dissolution

Hydroxyl radical generation has been observed during Cu CMP using hydrogen peroxide-glycine based slurries. While the Cu dissolution/polish rates increased with increasing glycine concentration, the copper dissolution rate decreased with increasing peroxide concentration indicating the occurrence of both dissolution and passive film formation during CMP. This is further confirmed by both in situ and ex situ electrochemical experiments.

scholarly journals Analyses of the Effect of Peptidoglycan on Photocatalytic Bactericidal Activity Using Different Growth Phases Cells of Gram-Positive Bacterium and Spheroplast Cells of Gram-Negative Bacterium

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Akane Saikachi ◽  
Kotone Sugasawara ◽  
Tomonori Suzuki
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radical ◽  
Survival Rates ◽  
Bactericidal Effect ◽  
Growth Phases ◽  
Intact Cells ◽  
E Coli ◽  
Bactericidal Effects ◽  
Photocatalytic Bactericidal Activity ◽  
Peptidoglycan Layer

We conducted photocatalytic experiments focusing on the peptidoglycan layer to elucidate the details of the mechanism of photocatalytic sterilization. The previous study of our laboratory suggested that the presence of the peptidoglycan layer increases the bactericidal effect. To further verify it, the following experiments were performed: experiments on cells with different peptidoglycan layer thickness used Lactobacillus plantarum cells with different growth phases, experiments on cells with the thin peptidoglycan layer used Escherichia coli cells and spheroplast cells from which the peptidoglycan layer was removed from E. coli cells. The bactericidal effects increased as the growth progresses of L. plantarum. It was confirmed by TEM that the thickness of the peptidoglycan layer increased with cell growth. The survival rates of E. coli intact cells were significantly lower than those of spheroplast cells. These results strongly suggest that the peptidoglycan layer enhances the photocatalytic bactericidal effect. As a result of allowing the photocatalytic reaction to act on peptidoglycan, the amount of hydroxyl radical was smaller, and the amount of hydrogen peroxide was higher than in the absence of peptidoglycan. It is suggested that peptidoglycan may convert produced hydroxyl radical to hydrogen peroxide.

scholarly journals Effect of sodium hypochlorite solution and gel with/without passive ultrasonic irrigation on Enterococcus faecalis, Escherichia coli and their endotoxins

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 642
Author(s):  
Amjad Abu Hasna ◽  
Laiana Pereira Da Silva ◽  
Fernanda Carvalho Pelegrini ◽  
Cláudia Luísa Ribeiro Ferreira ◽  
Luciane Dias de Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Ethylenediaminetetraacetic Acid ◽  
Lipoteichoic Acid ◽  
Sodium Hypochlorite Solution ◽  
Passive Ultrasonic Irrigation ◽  
E Coli ◽  
Significant Difference ◽  
Ultrasonic Irrigation

Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The purpose of this study was to evaluate the effect of NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on Enterococcus faecalis, Escherichia coli, and their endotoxins, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Methods: 40 human lower premolars were contaminated with E. coli (ATCC 25922) for 28 days and E. faecalis (ATCC 29212) for 21 days. Specimens were randomly divided into four groups: (1) 2.5% NaOCl irrigating the canals without PUI activation; (2) 2.5% NaOCl with PUI; (3) 3% NaOCl gel irrigating the canals without PUI; and (4) 3% NaOCl gel with PUI. 40 mL of irrigant was used for each group. PUI activation was carried out using E1-Irrisonic stainless-steel tip at 10% frequency. After treatment, all specimens were filled with 3mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3min and then washed with nonpyrogenic saline solution. Three samples were collected from the canals: S1, at baseline to confirm biofilm formation; S2 after treatment; and S3 after EDTA. Samples were assessed for E. coli and E. faecalis colony forming units, and LPS and LTA were assessed using chromogenic kinetic LAL assay and ELISA, respectively. Data were analyzed by Kruskal-Wallis, Friedmann and Dunn tests with α≤0.05. Results: All groups were effective in reducing the microbial load of E. coli and E. faecalis after treatment without a significant difference among the groups. NaOCl and NaOCl gel groups had no significant difference in reducing LPS and LTA. Statistically increased reduction was seen for NaOCL + PUI and NaOCl gel + PUI compared for groups without PUI. Conclusions: NaOCl gel has the same antimicrobial action of NaOCl solution and can partially detoxify endotoxins. PUI improves NaOCl (gel or solution) action over E. faecalis and E. coli and their endotoxins.

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