scholarly journals Potential anti-proliferative effects of chemical constituents and hemisynthetic derivatives from Scadoxus pseudocaulus (Amarillydaceae)

2020 ◽  
Vol 20 (1) ◽  
pp. 469-475
Author(s):  
Annie Laure Ngankeu Pagning ◽  
Jean-de-Dieu Tamokou ◽  
Bushra Taj Muhammad ◽  
David Ngnokam ◽  
Leon AzefackTapondjou ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Biological Significance ◽  
Esterification Reaction ◽  
Natural Isolate ◽  
Raji Cells ◽  
Anti Cancer ◽  
Burkitts Lymphoma ◽  
Natural Isolates

Background: Biological significance of Amaryllidaceae is well advocated from the literature. In Cameroon, plants from this fam- ily are routinely used for the cure of liver, cancer and cardiovascular diseases. To date, no scientific investigation corresponding to the anti-cancer activity of extracts and isolated compounds of Scadoxus pseudocaulus is available. Objective: Current study is focused to elaborate the anti-proliferative effects of natural isolates (compounds 1-6, 9) and hemi-synthetic analogs (compounds 7-8) extracted from S. pseudocaulu. Methods: Column chromatography of the ethyl acetate extract followed by purification of different fractions led to the isolation of seven compounds (1 – 6, 9). Esterification reaction of compound 6 was carried out using butyroyl chlorides and triethylamin to produce two derivatives (7 – 8). The cytotoxic activity was performed after staining of treated cells with florescent dye propid- ium iodide. Dead cells were detected using cytometer FL2 or FL3 channels/filters. Results: Trans-derivative of narciclasine (a natural isolate from S. pseudocaulus), was found to be most potent among all tested compounds. Its effects were more significant on low malignant follicular lymphoma (DoHH2 cells) as compared to highly ma- lignant (EBV infected) Burkitts lymphoma (Raji cells). Conclusion: From our results, narciclasine appears to hold the potential of a lead molecule that can be used to bridge the ther- apeutic gaps in cancer research. Keywords: Scadoxus pseudocaulus; Amaryllidaceae; 7-deoxy-trans-dihydronarciclasin; farrerol; derivatization; cytotoxic activity. 

scholarly journals Improvement of Bioactivity with Nanoparticle Fabrication: Cytotoxic Test of Ethanol, N-Hexane and Ethyl Acetate Extract from Red Galangal Rhizome (Alpinia purpurata (Vieill.) K. Schum) in Bulk and Nanoparticle size using BSLT Method

2018 ◽  
Vol 21 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Enny Fachriyah ◽  
Dewi Kusrini ◽  
Pratama Jujur Wibawa
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Nanoparticle Size ◽  
Anti Cancer ◽  
Hexane Extracts ◽  
Nanoparticle Fabrication ◽  
Alpinia Purpurata ◽  
Anti Fungal

Some of the secondary metabolites present in red algae are terpenoids, quinones, flavonoids, alkaloids, essential oils, diarylheptanoids, steroids, cardioglycosides, oils and fats, tannins, carbohydrates. Activity of rhizomes, leaves and flowers red leaf is as antimicrobial, anti-fungal, anti-oxidants, anti-tumor, anti-cancer and vasodilator. One way to improve the physical, chemical and bioactivity properties of natural compounds was to make them into nanoparticles. In this study, the isolation of bioactive compounds contained in red laos rhizome by maceration method using ethanol solvent was done, then partitioned with n-hexane and ethyl acetate. The extracts thus obtained are fabricated into nanoparticles. Extracts in bulk and nanoparticles were then tested for cytotoxic activity using BSLT method. Results of analysis with PSA showed that ethanol extract had size 410,8 nm, n-hexane extract 220,7 nm and ethyl acetate extract 208,3 nm. The results of cytotoxic tests showed that nanoparticle size increased cytotoxic activity. Ethyl acetate extract was most active compared to ethanol and n-hexane extracts with LC50values of 17.919; 84,956; 166,526 ppm. Whereas the nanoparticle size was respectively 10,491; 74,072 and 84,197 ppm. Cytotoxic activity increases with nanoparticle fabrication. 

QUANTITATIVE PHYTOCHEMICAL DETERMINATIONS AND IN-VITRO CYTOTOXIC ACTIVITY OF MEDICINAL PLANTS

2015 ◽  
Vol 1 (01) ◽  
pp. 17-21
Author(s):  
P. Ethalsha Robert ◽  
A. Malar Retna
Keyword(s):  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Chemical Constituents ◽  
Chloroform Extract ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Medicinal Uses ◽  
Anti Cancer ◽  
Cancer Activity

The present study evaluates in-vitro cytotoxic activity of active extracts of medicinal plants. It evaluates anti-cancer activity of most active extracts of I. pes-caprae and C. roseus. This study focuses on the total phenol content and the total flavanoid content of the plant and searches into its medicinal potency based on the total phenol and total flavanoid content. The anti-cancer activity of the extract is found to be more significant than one another.The cytotoxic activity of Ipomoea pes-caprae and C. roseus showed better results. Methanol extracts of aerial and root of Ipomoea pes-caprae possess maximum anticancer activity is found to be 61.77% and 65.55%. Chloroform extract of Catharanthus roseus root was also active against MCF- 7 cells by exhibiting 64.34% of activity. These plants may be a source of new antibiotic compounds.These findings enriches our knowledge of the chemical constituents that are responsible for the medicinal uses of the plant and the anti-cancer potential of selected plants.

Evaluation of antipyretic activity of Capsicum annuum by Brewer’s yeast method

2020 ◽  
Vol 9 (1) ◽  
pp. 11-13
Author(s):  
Dinesh Babu J
Keyword(s):  
Weight Loss ◽  
Capsicum Annuum ◽  
Chemical Constituents ◽  
Gastrointestinal Function ◽  
Standard Drug ◽  
Antipyretic Activity ◽  
Similar Chemical ◽  
Anti Cancer ◽  
Science Investigations ◽  
Crucial Ingredient

Plants are being used in medicine and treatment of diseases from years in the evolution. During the development of science, investigations to establish eh scientific proof and validation for the activities and mechanism of herbs to treat diseases. Capsicum annuum has anti-cancer properties, analgesic properties, anti lithogenic and thermogenic properties. It is used to alter the gastrointestinal function and boost metabolism. Many formulations are enriched with capsaicin which is a crucial ingredient in Capsicum to exhibit its weight loss properties. Based on the anti-inflammatory and analgesic properties of the plant and taking into consideration, the current research will focus on the investigation and comparison of the antipyretic activity of Capsicum in fruits and leaves. Results show that the leaves have similar chemical constituents that had antipyretic potential compared to the fruits. Overall, the Capsicum showed a comparatively same activity with the standard drug, paracetamol.

Ethnomedicinal uses, Phytochemistry and Pharmacological Aspects of the Genus Berberis Linn: A Comprehensive Review

2020 ◽  
Vol 23 ◽  
Author(s):  
Roohi Mohi-ud-din ◽  
Reyaz Hassan Mir ◽  
Prince Ahad Mir ◽  
Saeema Farooq ◽  
Syed Naiem Raza ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Pharmacological Activities ◽  
Traditional Use ◽  
Comprehensive Review ◽  
Wide Range ◽  
Anti Cancer ◽  
Comprehensive Survey ◽  
Ethnomedicinal Uses

Background: Genus Berberis (family Berberidaceae), which contains about 650 species and 17 genera worldwide, has been used in folklore and various traditional medicine systems. Berberis Linn. is the most established group among genera with around 450-500 species across the world. This comprehensive review will not only help researchers for further evaluation but also provide substantial information for future exploitation of species to develop novel herbal formulations. Objective: The present review is focussed to summarize and collect the updated review of information of Genus Berberis species reported to date regarding their ethnomedicinal information, chemical constituents, traditional/folklore use, and reported pharmacological activities on more than 40 species of Berberis. Conclusion: A comprehensive survey of the literature reveals that various species of the genus possess various phytoconstituents mainly alkaloids, flavonoid based compounds isolated from different parts of a plant with a wide range of pharmacological activities. So far, many pharmacological activities like anti-cancer, anti-hyperlipidemic, hepatoprotective, immunomodulatory, anti-inflammatory both in vitro & in vivo and clinical study of different extracts/isolated compounds of different species of Berberis have been reported, proving their importance as a medicinal plant and claiming their traditional use.

Phytochemical, Pharmacological Activities and Ayurvedic Significances of Magical Plant Mimosa pudica Linn

2020 ◽  
Vol 17 ◽  
Author(s):  
Vijay Kumar
Keyword(s):  
Therapeutic Potential ◽  
Wide Spectrum ◽  
Chemical Constituents ◽  
Future Research ◽  
Mimosa Pudica ◽  
Pharmacological Activities ◽  
Medicinal Uses ◽  
Traditional Medicines ◽  
Crude Extracts ◽  
Anti Cancer

: Mimosa pudica Linn is an integrated part of Traditional Medicines Systems of India, China, Africa, Korea and America. It has been used from centuries in traditional medicines to cure different diseases like fever, diabetes, constipation, jaundice, ulcers, biliousness, and dyspepsia. It is an important ingredient of wide class of herbal formulations. To assess the scientific evidence for therapeutic potential of Mimosa pudica Linn and to identify the gaps for future research. The available information on the ethno-medicinal uses, phytochemistry, pharmacology and toxicology of Mimosa pudica Linn was collected via a library and electronic searches in Sci-Finder, Pub-Med, Science Direct, Google Scholar for the period, 1990 to 2020. In traditional medicinal systems, variety of ethno-medicinal applications of Mimosa pudica Linn has been noticed. Phytochemical investigation has resulted in identification of 40 well known chemical constituents, among which alkaloids, phenols and flavionoids are the predominant groups. The crude extracts and isolates have exhibited a wide spectrum of in vitro and in vivo pharmacological activities including anti-cancer, anti-inflammation, osteoporosis, neurological disorders, hypertension etc.. To quantify the Mimosa pudica Linn and its formulations, analytical techniques like HPLC and HPTLC has shown dominancy with good range of recovery and detection limit. Mimosa pudica Linn is the well-known herb since an ancient time. The pharmacological results supported some of the applications of Mimosa pudica Linn in traditional medicine systems. Perhaps, the predominance of alkaloids, phenols and flavionoids are responsible for the pharmacological activities the crude extracts and isolates of Mimosa pudica Linn. Further, there is need to isolate and evaluate the active chemical constituents of Mimosa pudica Linn having significant medicinal values. In future, it is important to study the exact mechanism associated with the phytochemicals of Mimosa pudica Linn especially on anti-cancer activities. Notably, toxicity studies on Mimosa pudica Linn are limited which are to be explored in future for the safe application of Mimosa pudica Linn and its formulations.

Synthesis, Anti-proliferative Evaluation, and Molecular Docking Studies of 3-(alkylthio)-5,6-diaryl-1,2,4-triazines as Tubulin Polymerization Inhibitors

2019 ◽  
Vol 16 (11) ◽  
pp. 1194-1201 ◽  
Author(s):  
Farhad Saravani ◽  
Ebrahim Saeedian Moghadam ◽  
Hafezeh Salehabadi ◽  
Seyednasser Ostad ◽  
Morteza Pirali Hamedani ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Cytotoxic Activity ◽  
Binding Site ◽  
Cell Line ◽  
Cell Lines ◽  
Tubulin Polymerization ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Colchicine Binding Site ◽  
Anti Cancer

Background: The role of microtubules in cell division and signaling, intercellular transport, and mitosis has been well known. Hence, they have been targeted for several anti-cancer drugs. Methods: A series of 3-(alkylthio)-5,6-diphenyl-1,2,4-triazines were prepared and evaluated for their cytotoxic activities in vitro against three human cancer cell lines; human colon carcinoma cells HT-29, human breast adenocarcinoma cell line MCF-7, human Caucasian gastric adenocarcinoma cell line AGS as well as fibroblast cell line NIH-3T3 by MTT assay. Docking simulation was performed to insert these compounds into the crystal structure of tubulin at the colchicine binding site to determine a probable binding model. Compound 5d as the most active compound was selected for studying of microtubule disruption. Results: Compound 5d showed potent cytotoxic activity against all cell lines. The molecular modeling study revealed that some derivatives of triazine strongly bind to colchicine binding site. The tubulin polymerization assay kit showed that the cytotoxic activity of 5d may be related to inhibition of tubulin polymerization. Conclusion: The cytotoxicity and molecular modeling study of the synthesized compounds with their inhibition activity in tubulin polymerization demonstrate the potential of triazine derivatives for development of new anti-cancer agents.

Design, Synthesis, In vitro Cytotoxic Activity Evaluation, and Study of Apoptosis Inducing Effect of New Styrylimidazo[1,2-a]Pyridines as Potent Anti-Breast Cancer Agents

2019 ◽  
Vol 19 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Faeze Khalili ◽  
Sara Akrami ◽  
Malihe Safavi ◽  
Maryam Mohammadi-Khanaposhtani ◽  
Mina Saeedi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
High Yield ◽  
Breast Cancer Cell Lines ◽  
Pyridine Derivatives ◽  
One Pot ◽  
Standard Drug ◽  
Three Component Reaction ◽  
Anti Cancer

Background: This paper reports synthesis, cytotoxic activity, and apoptosis inducing effect of a novel series of styrylimidazo[1,2-a]pyridine derivatives. Objective: In this study, anti-cancer activity of novel styrylimidazo[1,2-a]pyridines was evaluated. Methods: Styrylimidazo[1,2-a]pyridine derivatives 4a-o were synthesized through a one-pot three-component reaction of 2-aminopyridines, cinnamaldehydes, and isocyanides in high yield. All synthesized compounds 4a-o were evaluated against breast cancer cell lines including MDA-MB-231, MCF-7, and T-47D using MTT assay. Apoptosis was evaluated by acridine orange/ethidium bromide staining, cell cycle analysis, and TUNEL assay as the mechanism of cell death. Results: Most of the synthesized compounds exhibited more potent cytotoxicity than standard drug, etoposide. Induction of apoptosis by the most cytotoxic compounds 4f, 4g, 4j, 4n, and 4m was confirmed through mentioned methods. Conclusion: In conclusion, these results confirmed the potency of styrylimidazo[1,2-a]pyridines for further drug discovery developments in the field of anti-cancer agents.

scholarly journals Chemical Constituents of the Marine Sponge Aaptos aaptos (Schmidt, 1864) and Their Cytotoxic Activity

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199334
Author(s):  
Do Thi Trang ◽  
Bui Huu Tai ◽  
Dan Thuy Hang ◽  
Pham Hai Yen ◽  
Phan Thi Thanh Huong ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Spectral Data ◽  
Absolute Configuration ◽  
Marine Sponge ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Extensive Analysis ◽  
First Time ◽  
Tddft Method ◽  
Mcf 7

Seven compounds (1-7) were isolated from the marine sponge Aaptos aaptos living in the Vietnamese sea. Their structures were determined as 2 hours, 5 H,7 H,9 H-9 S-hydroxy-imidazo[1,5- α]pyridine-1,3-dione (1), 3-([9-methylhexadecyl]oxy)propane-1,2-diol 2, 2,3-dihydro-2,3-dioxoaaptamine (3), indol-3-aldehyde (4), methyl indole-3-carboxylate (5) 4-hydroxy-5-(indole-3-yl)−5-oxo-pentan-2-one (6), and thymidine (7) by extensive analysis of HR-ESI-MS, 1D, and 2D NMR spectral data, as well as by comparison of the spectral data with those reported in the literature. In addition, the absolute configuration of 1 was determined from the experimental ECD spectrum and comparison of this with the theoretical ECD calculations using the TDDFT method. Compounds 1 and 2 were isolated from nature for the first time. Compound 3 induced cytotoxic activity against SK-LU-1, MCF-7, HepG2, and SK-Mel-2 cell lines with IC50 values of 41.27 ± 2.63, 40.70 ± 2.65, 34.31 ± 3.43, and 36.63 ± 1.40 µM, respectively.

Chemical constituents and cytotoxic activity of Stachys pilifera Benth.

2021 ◽  
Vol 139 ◽  
pp. 226-229
Author(s):  
Azadeh Khademian ◽  
Marzieh Tabefam ◽  
Zeinab Mazarei ◽  
Mohammad Reza Kanani ◽  
Houri Sepehri ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Chemical Constituents
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