Phytochemical and antibacterial screening of leaf extracts from Cassia singueana Del.(Fabaceae)

Justicia Adhatoda is one of the medicinal plant native to Asia that is widely used in antidiabetic, anti inflammatory, antibacterial properties, treatment of cough and other respiratory ailments. In the present study, few steps procedure method was used to extract the major compound in the leaves.From the leaf extracts, phytochemical tests, measurement of antioxidant activity andantibacterial activities were studied. The results showed that the ethanol leaf extracts contains Phenols, Flavonoids, Tannis, Alkaloids and Saponins. The antioxidant activitywas evaluated in-vitro by radical scavenging DPPH, FRAP and H2O2 method and the disc diffusion method was employed for the antibacterial study on Pseudomonas, Staphylococcus, Bacillus, E.coli using chloramphinicol antibiotic as standard. Antibacterial screening showed that the maximum zone of inhibition for ethanolic extract against E.Coli is around 1.35 cm.

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The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine)Gardenia jasminoidesEllis

The Scientific World JOURNAL ◽

10.1155/2014/407284 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Reza Farzinebrahimi ◽

Rosna Mat Taha ◽

Kamaludin Rashid ◽

Jamilah Syafawati Yaacob

Keyword(s):

Inhibitory Effect ◽

Root Induction ◽

Inhibition Activity ◽

Fresh Weight ◽

Leaf Extracts ◽

E Coli ◽

Antibacterial Screening ◽

Gardenia Jasminoides ◽

Metabolic Activities

The leaf ofGardenia jasminoidesEllis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L−1) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L−1) respectively were used. Leaf segments cultured on WPM media added with Kn (1 mg L−1) and TDZ (2 mg L−1) yielded the least amount of callus. It was found that WPM media added with IAA (4.5–5.0 mg L−1) were optimum for root induction fromG.jasminoidesplantlets. Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect againstE. coli,P. aeruginosa,S. aureus,andB. cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity againstE. coliandB. cereus. The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts.

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Assessment of phytochemical standardization and antibacterial screening of leaf extracts of Biophytum sensitivum

Journal of Xidian University ◽

10.37896/jxu14.9/057 ◽

2020 ◽

Vol 14 (9) ◽

Keyword(s):

Leaf Extracts ◽

Antibacterial Screening

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Antibacterial screening of the stem bark and leaf extracts of Litsea glutinosa (Lour.) C.B. Rob - an ethnomedicinally important tree of the Western Ghats

Pharmacognosy Journal ◽

10.5530/pj.2011.21.13 ◽

2011 ◽

Vol 3 (21) ◽

pp. 72-76 ◽

Cited By ~ 7

Author(s):

K. Pradeepa ◽

V. Krishna ◽

Venkatesh ◽

K. Girish Kumar ◽

B.V. Thirumalesh ◽

...

Keyword(s):

Western Ghats ◽

Stem Bark ◽

Leaf Extracts ◽

Antibacterial Screening ◽

Litsea Glutinosa ◽

The Western Ghats

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Phytochemical and Antibacterial Screening on Leaf Extracts of Vitex negundo Linn

Indian Journal Of Applied Research ◽

10.15373/2249555x/july2013/185 ◽

2011 ◽

Vol 3 (7) ◽

pp. 585-587

Author(s):

P. Selvaganesh P. Selvaganesh ◽

◽

D. Nancy D. Nancy ◽

D. Kamalnathan D. Kamalnathan

Keyword(s):

Vitex Negundo ◽

Leaf Extracts ◽

Antibacterial Screening

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Callus induction studies on Stevia rebaudiana and antibacterial screening of leaf extracts

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.1(4).p208-211 ◽

2011 ◽

Vol 1 (4) ◽

pp. 208-211

Author(s):

S. Pande ◽

M. Wani ◽

Madhukar Khetmalas

Keyword(s):

Callus Induction ◽

Leaf Explants ◽

Stevia Rebaudiana ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Leaf Extracts ◽

Antibacterial Screening ◽

Dichlorophenoxy Acetic Acid ◽

Asteraceae Family

The medicinal plant Stevia rebaudiana (Asteraceae family) produces sweetsteviol glycosides in the leaves which can be used as a natural alternative toartificial sweetener. The propagation by seeds or other methods is not viabledue to the low rate of seed germination. The present study was focused oninduction and maintenance of callus from leaf explants of Stevia by usingdifferent concentrations of 2, 4 dichlorophenoxy acetic acid . Surface sterilizedleaf explants were inoculated in MS medium with vitamins, sucrose (30g l –1), agar (0.8% w/v) and supplemented with various concentrations of 2, 4Ã¢â‚¬ÂD (0.5, 1.0, 1.5, 2.0, 2.5 and 3 mg l –1). Medium supplemented with 2.5 mg l–12, 4 Ã¢â‚¬ÂD showed best callus induction. Leaf extracts in different solvent systemswere screened for antimicrobial activity against some Gram Ã¢â‚¬Âpositiveand Gram Ã¢â‚¬Ânegative bacteria by disc diffusion method. The chloroform andmethanol extract of leaves exhibited a concentration dependent antibacterialinhibition.

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