The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine)Gardenia jasminoidesEllis

The leaf ofGardenia jasminoidesEllis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L−1) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L−1) respectively were used. Leaf segments cultured on WPM media added with Kn (1 mg L−1) and TDZ (2 mg L−1) yielded the least amount of callus. It was found that WPM media added with IAA (4.5–5.0 mg L−1) were optimum for root induction fromG.jasminoidesplantlets. Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect againstE. coli,P. aeruginosa,S. aureus,andB. cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity againstE. coliandB. cereus. The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts.

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The Phytochemical Analysis of Vinca L. Species Leaf Extracts Is Correlated with the Antioxidant, Antibacterial, and Antitumor Effects

Molecules ◽

10.3390/molecules26103040 ◽

2021 ◽

Vol 26 (10) ◽

pp. 3040

Author(s):

Alexandra Ciorîță ◽

Cezara Zăgrean-Tuza ◽

Augustin C. Moț ◽

Rahela Carpa ◽

Marcel Pârvu

Keyword(s):

Bacterial Infections ◽

Inhibitory Effect ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Antitumor Effects ◽

Cytotoxic Effects ◽

E Coli ◽

Lactate Dehydrogenase Release ◽

Nitric Oxide Concentration

The phytochemical analysis of Vinca minor, V. herbacea, V. major, and V. major var. variegata leaf extracts showed species-dependent antioxidant, antibacterial, and cytotoxic effects correlated with the identified phytoconstituents. Vincamine was present in V. minor, V. major, and V. major var. variegata, while V. minor had the richest alkaloid content, followed by V. herbacea. V. major var. variegata was richest in flavonoids and the highest total phenolic content was found in V. herbacea which also had elevated levels of rutin. Consequently, V. herbacea had the highest antioxidant activity followed by V. major var. variegata. Whereas, the lowest one was of V. major. The V. minor extract showed the most efficient inhibitory effect against both Staphylococcus aureus and E. coli. On the other hand, V. herbacea had a good anti-bacterial potential only against S. aureus, which was most affected at morphological levels, as indicated by scanning electron microscopy. The Vinca extracts acted in a dose-depended manner against HaCaT keratinocytes and A375 melanoma cells and moreover, with effects on the ultrastructure, nitric oxide concentration, and lactate dehydrogenase release. Therefore, the Vinca species could be exploited further for the development of alternative treatments in bacterial infections or as anticancer adjuvants.

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LARVICIDAL ACTIVITY OF LANTANA INDICA AND VITEX NEGUNDO ON CULEX QUINQUEFASCIATUS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i5.25040 ◽

2018 ◽

Vol 11 (5) ◽

pp. 414

Author(s):

Rathnasagar K ◽

Thiyagaraj Anand

Keyword(s):

Larvicidal Activity ◽

Culex Quinquefasciatus ◽

Binding Protein ◽

Inhibitory Effect ◽

Negative Control ◽

Vitex Negundo ◽

Leaf Extracts ◽

Active Compounds ◽

Sterol Binding

Objectives: The activity of two different leaf extracts of Lantana indica and Vitex negundo is tested against the 3rd and 4th instar Culex quinquefasciatus larvae to evaluate the potency of the extracts as a larvicide and to find an ecologically sustainable alternative to chemical insecticides. A bioinformatics screening approach was performed to evaluate the in vivo results.Methods: The obtained larvae’s from nearby water sources were tested with N, N-diethyl-meta-toluamide (DEET) as the positive control which is the commercial chemical mosquito repellent and the solvents used for the respective plant extracts act as the negative control. Petroleum ether (PE), ethyl acetate (EA) and an aqueous (AQ) extract were prepared for both L. indica and V. negundo extracts, and its larvicidal activity was tested. A docking based approach was used to study the inhibitory effect of known active compounds from L. indica and V. negundo against acetylcholine esterase (AChE) and sterol binding protein as targets.Results: On comparing the results between three plants extract for its larvicidal activity, the EA extract of V. negundo and L. indica is found to be potent with a low LC50 value. Further, the docking studies between active compounds of L. indica and V. negundo with AChE and Sterol binding protein as targets showed that the compound tangeritin-1 had a good docking score compared to DEET and could be a natural alternative for larvicidal activity in the mosquito.Conclusion: Individual activity of tangeritin-1 could be further studied with mosquito mortality studies and molecular simulations and develop tangeritin-1 as a potential larvicidal compound for commercial use.

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Role of F1C Fimbriae, Flagella, and Secreted Bacterial Components in the Inhibitory Effect of Probiotic Escherichia coli Nissle 1917 on Atypical Enteropathogenic E. coli Infection

Infection and Immunity ◽

10.1128/iai.01431-13 ◽

2014 ◽

Vol 82 (5) ◽

pp. 1801-1812 ◽

Cited By ~ 23

Author(s):

Sylvia Kleta ◽

Marcel Nordhoff ◽

Karsten Tedin ◽

Lothar H. Wieler ◽

Rafal Kolenda ◽

...

Keyword(s):

Escherichia Coli ◽

Molecular Mechanisms ◽

Inhibitory Effect ◽

Host Cells ◽

Single Infection ◽

Content Type ◽

E Coli ◽

Initial Adhesion

ABSTRACTEnteropathogenicEscherichia coli(EPEC) is recognized as an important intestinal pathogen that frequently causes acute and persistent diarrhea in humans and animals. The use of probiotic bacteria to prevent diarrhea is gaining increasing interest. The probioticE. colistrain Nissle 1917 (EcN) is known to be effective in the treatment of several gastrointestinal disorders. While bothin vitroandin vivostudies have described strong inhibitory effects of EcN on enteropathogenic bacteria, including pathogenicE. coli, the underlying molecular mechanisms remain largely unknown. In this study, we examined the inhibitory effect of EcN on infections of porcine intestinal epithelial cells with atypical enteropathogenicE. coli(aEPEC) with respect to single infection steps, including adhesion, microcolony formation, and the attaching and effacing phenotype. We show that EcN drastically reduced the infection efficiencies of aEPEC by inhibiting bacterial adhesion and growth of microcolonies, but not the attaching and effacing of adherent bacteria. The inhibitory effect correlated with EcN adhesion capacities and was predominantly mediated by F1C fimbriae, but also by H1 flagella, which served as bridges between EcN cells. Furthermore, EcN seemed to interfere with the initial adhesion of aEPEC to host cells by secretion of inhibitory components. These components do not appear to be specific to EcN, but we propose that the strong adhesion capacities enable EcN to secrete sufficient local concentrations of the inhibitory factors. The results of this study are consistent with a mode of action whereby EcN inhibits secretion of virulence-associated proteins of EPEC, but not their expression.

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Experimental Application of Sage in Rabbit Husbandry

Acta Veterinaria Brno ◽

10.2754/avb200877040581 ◽

2008 ◽

Vol 77 (4) ◽

pp. 581-588 ◽

Cited By ~ 10

Author(s):

R. Szabóová ◽

A. Lauková ◽

Ľ. Chrastinová ◽

M. Simonová ◽

V. Strompfová ◽

...

Keyword(s):

Inhibitory Effect ◽

Negative Influence ◽

Feed Consumption ◽

Control Group ◽

Coagulase Negative Staphylococci ◽

E Coli ◽

Commercial Feed ◽

Experimental Group

Salvia spp. belongs to the Labiatae family and is characterized by antimicrobial and antiinflammatory effect. The aim of this study was to test its in vitro and in vivo inhibitory effect against bacteria as well as to find an alternative possibility to use sage in the rabbit ecosystem examining biochemical, zootechnical and inmunological indicators, compared to the commercial feed mixture Xtract. Using the sage extract in in vitro tests, its inhibitory effect was noted. Under in vivo conditions, in the experimental group with sage (EG1), reduction of Pseudomonas-like sp. (p < 0.01) and E. coli (p < 0.01) was noted after 7 days of sage application compared to the control group CG2 (with Robenidin) as well as after 21 days of sage extract application, when the reduction of coagulase-negative staphylococci (p < 0.01) was detected (in comparison with the experimental group-EG2, Xtract group). In the caecum of rabbits from EG1, higher values of lactic, acetic and butyric acids were noted. The values of propionic acid were not influenced. Biochemical indicators were not influenced; however, the values of GSH Px were lower in EG1 compared to EG2. Higher phagocytic activity (18%) was noted in EG1 than in EG2 (13%) after 21 days of additives application. The reduction of Eimeria sp. oocysts was demonstrated in EG1 (sage group) after 7 days of sage application comparing to CG2 (217 OPG to 566 OPG). The animals in both experimental groups achieved higher feed consumption and weight gain, lower mortality compared to both controls. Neither of the additives had a negative influence on the health status and growth performance of rabbits.

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Evaluation of three plant species to control black scurf disease of Irish potato (Solanum tuberosum Linn.)

Notulae Scientia Biologicae ◽

10.15835/nsb12110643 ◽

2020 ◽

Vol 12 (1) ◽

pp. 90-99

Author(s):

Matthew O. ADEBOLA ◽

Tunde S. BELLO ◽

Esther A. SERIKI ◽

Mariam B. AREMU

Keyword(s):

Solanum Tuberosum ◽

Mycelial Growth ◽

Pathogenic Fungus ◽

Inhibitory Effect ◽

Black Scurf ◽

Irish Potato ◽

Diffusion Method ◽

Leaf Extracts

Irish potato (Solanum tuberosum) is an important worldwide food crop and one of the most popular in Nigeria. Its abundance and successful yield have been immensely affected by black scurf disease caused by Rhizoctonia solani. Harnessing a cost-effective management of this pathogenic fungus, three botanical species Acalypha wilkesiana, Moringa oleifera and Carica papaya leaves, each at concentrations of 0 mg ml-1 (control), 25 mg ml-1, 50 mg ml-1 and 75 mg ml-1 were evaluated in vitro. The plant leaf extracts were prepared using methanol and were evaluated for their toxicity using agar well diffusion method. The fungus was isolated from spoilt Irish potato with black scurf symptoms. The results showed the presence of some phytochemicals in leaf extract of each of the plants tested. The three leaves extract independently inhibited mycelial growth of R. solani. The potency of all the plant extracts increased with the increase in concentration. The highest concentration (75 mg ml-1) of M. oleifera and C. papaya evaluated, gave the highest inhibitory effect of 0.81 mm and 1.63 mm respectively, which were not significantly different (p> 0.05), but was obviously different from A. wilkesiana (2.81 mm). Furthermore, M. oleifera extract gave the highest percentage of mycelial growth inhibition of the fungus in all grades of the concentrations evaluated, whereas A. wilkesiana showed the least. The leaves of the three species are therefore recommended for in vivo control of this fungus, owing to their proven efficacy and to their cheap availability.

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THE ACTION OF SULFONAMIDES ON THE RESPIRATION OF BACTERIA AND YEAST

The Journal of General Physiology ◽

10.1085/jgp.25.6.805 ◽

1942 ◽

Vol 25 (6) ◽

pp. 805-817 ◽

Cited By ~ 8

Author(s):

M. G. Sevag ◽

M. Shelburne ◽

Stuart Mudd

Keyword(s):

Structural Similarity ◽

Inhibitory Effect ◽

Brewer’S Yeast ◽

Respiratory Enzymes ◽

Staph Aureus ◽

E Coli ◽

Brewer's Yeast ◽

Inhibiting Effect

The inhibiting effects of sulfonamide drugs and their derivatives on the anaerobic decarboxylation of pyruvic acid by Staphylococcus aureus, Escherichia coli, baker's and brewer's yeast, and a carboxylase preparation from brewer's yeast have been investigated. These drugs are: sulfanilamide, sulfapyridine, sulfadiazine, sulfamethyldiazine, sulfathiazole, sulfamethylthiazole, sulfanilamido-5-ethyl-4-thiazolone, 2-aminopyrimidine, 2-aminothiazole, and 2-aminopyridine. The sulfathiazole ring appears to exercise decidedly greater specific inhibiting effect on the carboxylases of Staph. aureus and E. coli. The inhibiting effect on yeast carboxylase is non-differentiable among all the substances tried, except sulfamethyldiazine which is completely ineffective on the carboxylases of the organisms studied. The specific inhibitory effect of sulfathiazole on the carboxylases of Staph. aureus and E. coli in comparison to sulfanilamide, sulfapyridine, and sulfadiazine is in harmony with in vivo and in vitro experimental results of other investigators. The results of the present investigation appear to support the hypothesis (1) that sulfonamides exert their bacteriostatic action through chemical affinity for the carrier proteins of certain respiratory enzymes of the bacterial cell, and that this affinity may in part be related to structural similarity between components of the drugs and the corresponding respiratory coenzymes.

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Inhibition of isoleucyl-transfer ribonucleic acid synthetase in Echerichia coli by pseudomonic acid

Biochemical Journal ◽

10.1042/bj1760305 ◽

1978 ◽

Vol 176 (1) ◽

pp. 305-318 ◽

Cited By ~ 135

Author(s):

Julia Hughes ◽

Graham Mellows

Keyword(s):

Rna Synthesis ◽

Inhibitory Effect ◽

Trna Synthetase ◽

Threonine Deaminase ◽

Trna Synthetases ◽

E Coli ◽

Naturally Occurring ◽

Pseudomonic Acid

The mode of action of the antibiotic pseudomonic acid has been studied in Escherichia coli. Pseudomonic acid strongly inhibits protein and RNA synthesis in vivo. The antibiotic had no effect on highly purified DNA-dependent RNA polymerase and showed only a weak inhibitory effect on a poly(U)-directed polyphenylalanine-forming ribosomal preparation. Chloramphenicol reversed inhibition of RNA synthesis in vivo. Pseudomonic acid had little effect on RNA synthesis in a regulatory mutant, E. coli B AS19 RCrel, whereas protein synthesis was strongly inhibited. In pseudomonic acid-treated cells, increased concentrations of ppGpp, pppGpp and ATP were observed, but the GTP pool size decreased, suggesting that inhibition of RNA synthesis is a consequence of the stringent control mechanism imposed by pseudomonic acid-induced deprivation of an amino acid. Of the 20 common amino acids, only isoleucine reversed the inhibitory effect in vivo. The antibiotic was found to be a powerful inhibitor of isoleucyl-tRNA synthetase both in vivo and in vitro. Of seven other tRNA synthetases assayed, only a weak inhibitory effect on phenylalanyl-tRNA synthetase was observed; this presumably accounted for the weak effect on polyphenylalanine formation in a ribosomal preparation. Pseudomonic acid also significantly de-repressed threonine deaminase and transaminase B activity, but not dihydroxyacid dehydratase (isoleucine-biosynthetic enzymes) by decreasing the supply of aminoacylated tRNAIle. Pseudomonic acid is the second naturally occurring inhibitor of bacterial isoleucyl-tRNA synthetase to be discovered, furanomycin being the first.

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Inhibitory effect of Zanthoxylum bungeanum essential oil (ZBEO) on Escherichia coli and intestinal dysfunction

Food & Function ◽

10.1039/c6fo01739h ◽

2017 ◽

Vol 8 (4) ◽

pp. 1569-1576 ◽

Cited By ~ 8

Author(s):

Lei Hong ◽

Wu Jing ◽

Wang Qing ◽

Su Anxiang ◽

Xue Mei ◽

...

Keyword(s):

Escherichia Coli ◽

Essential Oil ◽

Inhibitory Effect ◽

Intestinal Health ◽

Inhibitory Effects ◽

E Coli ◽

Zanthoxylum Bungeanum

The inhibitory effects of Zanthoxylum bungeanum essential oil (ZBEO) on Escherichia coli (E. coli) in vitro and in vivo were investigated, as well as its function of improvement of intestinal health.

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Evaluation of antimicrobial activity of magnesium oxide nanocomposite film in combination with ε-poly-L-lysine against Foodborne pathogens in vacuum packaged beef

10.21203/rs.3.rs-294697/v1 ◽

2021 ◽

Author(s):

Masumeh Samadi ◽

Seyed Shahram Shekarforoush ◽

Hamid Reza Gheisari

Keyword(s):

Antimicrobial Activity ◽

Magnesium Oxide ◽

Culture Media ◽

Antimicrobial Effect ◽

Inhibitory Effect ◽

Melt Mixing ◽

E Coli ◽

Mgo Nanoparticle

Abstract Background: The aim of this study was to evaluate the antimicrobial activity of magnesium oxide nanocomposite (MgO NC) film based on Low-density polyethylene (LDPE) alone or in combination with three concentrations of ε-poly-L-lysine (500, 1000 and 2000 µg/ml) on Escherichia coli O157:H7 and Listeria monocytogenes in culture media and fresh beef. Methods: MgO NC film were prepared by melt mixing LDPE and MgO nanoparticle in the extruder. For in vitro antibacterial analysis, the MgO NC film alone or in combination with polylysine were evaluated in tryptic soy broth for 5 days at 37 °C. For in vivo analysis, beef samples were inoculated with the selected bacteria and packaged in MgO NC film under vacuum and stored at 4 °C and evaluated for up to 20 days. Results: Polylysine had an antibacterial effect against E. coli and L. monocytogenes in TSB. But MgO NC film had a bacteriostatic effect only against E. coli. MgO NC film inhibited the growth of E. coli on the surface of beef samples. Pollysine at concentrations of 500 µg/ml or more showed inhibitory activity against E. coli and L. monocytogenes in beef samples. No additional reduction was observed by combining the different concentrations of polylysine with MgO NC film.Conclusions: Polylysine at all concentrations had an inhibitory effect on E. coli and L. monocytogenes in the culture medium and beef. Although the migration of MgO nanoparticle from the film to beef was very low, but as it has little antimicrobial effect, it is not recommended as a suitable package for improving the safety of raw beef.

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UTILIZATION OF SAMBUNG NYAWA LEAF EXTRACTS Gynura procumbens (Lour) Merr. FOR TREATMENT OF Vibrio alginolyticus IN TIGER GROUPER (Epinephelus fuscoguttatus Forsskal, 1775)

Berkala Perikanan Terubuk ◽

10.31258/terubuk.47.2.134-150 ◽

2019 ◽

Vol 47 (2) ◽

pp. 134

Author(s):

Novi Santika ◽

Wardiyanto Wardiyanto ◽

Esti Harpeni

Keyword(s):

Leaf Extract ◽

Sea Water ◽

Economic Value ◽

In Vitro Study ◽

Inhibitory Effect ◽

Vibrio Alginolyticus ◽

Leaf Extracts ◽

Tiger Grouper

Tiger Grouper is one of the sea water fish commodities that is quite popular with the community and has a high economic value. The problem faced by farmers is the attack of Vibriosis, one of which is caused by the Vibrio alginolyticus bacteria. The use of synthetic antibiotics has been widely used but has many adverse effects, so it needs new alternatives for the treatment of Vibriosis disease. One of them is by using the extract of lifelong leaf extract. Life-sustaining plants (Gynura procumbens) contain secondary metabolites such as flavonoids, tannins, and antibacterial saponins. This study aims to determine the best dosage of lifelong leaf extract for the treatment of Vibriosis disease in tiger grouper. The study was conducted in two stages, namely in vitro and in vivo. Before the fish were treated with feed that had been given a sambung deca leaf extract, the fish were challenged using Vibrio alginolyticus with a density of 108 CFU / mL as much as 0.1 mL / head and then fed with treatment and maintained for 21 days. The results of the in vitro study showed that the life of sambung leaf extract at a dose of 700 ppm had a broad inhibitory effect on V. alginolyticus, which amounted to 10.47 mm compared to other treatments. Whereas when continued for in vivo testing, a dose of 350 ppm in general has been applied for the treatment of attacks of Vibrio alginolyticus in tiger grouper.

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