GC/MS analysis of the essential oils of Cupressus arizonica Greene, Juniperus communis  L. and Mentha longifolia L.

Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.

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Antimicrobial and antioxidant activities and gas chromatography mass spectrometry (GC/MS) analysis of the essential oils of Ajuga bracteosa Wall. ex Benth. and Lavandula dentata L. growing wild in Yemen

Journal of Medicinal Plants Research ◽

10.5897/jmpr11.1565 ◽

2012 ◽

Vol 6 (15) ◽

Cited By ~ 1

Author(s):

Ramzi A. Mothana

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oils ◽

Antioxidant Activities ◽

Gas Chromatography Mass Spectrometry ◽

Chromatography Mass Spectrometry ◽

Ms Analysis

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ANTIOXIDANT AND ANTITYROSINASE ACTIVITIES OF PIPER MAINGAYI HK. ESSENTIAL OILS

Jurnal Teknologi ◽

10.11113/jt.v78.4912 ◽

2015 ◽

Vol 78 (1) ◽

Author(s):

Nur Athirah Hashim ◽

Farediah Ahmad ◽

Deny Susanti

Keyword(s):

Essential Oils ◽

Chemical Components ◽

Caryophyllene Oxide ◽

Volatile Oils ◽

Ms Analysis ◽

Sesquiterpene Hydrocarbons ◽

Fruit Oil

The study was aimed to investigate the chemical components of stem and fruit volatile oils of Piper. maingayi Hk. with their antioxidant and antityrosinase activities. GC and GC-MS analysis of the essential oils obtained from the fresh stem and fruit of P. maingayi Hk. resulted in the identification of 34 and 18 components accounting for 83.6% and 78.7% of the total amount, respectively. Sesquiterpene hydrocarbons and oxygenated sesquiterpenes were the most highly represented classes as the former ranging from 64.7% to 70.7%, and the latter varying from 7.4% to 8.6%. The main constituents of the stem oil were: β-caryophyllene (26.2%), a-cedrene (8.4%), caryophyllene oxide (6.7%) and cis-calamenene (6.2%), while the fruit oil was dominated by δ-cadinene (22.6%), β-caryophyllene (18.8%), a-copaene (11.2%) and a-cadinol (7.1%). The stem and fruit of P. maingayi oils showed significant value for antioxidant and antityrosinase activities.

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Medicinal and pesticidal potentials of the constituents of the essential oil from Adenia cissampeloides leaves

Kemija u industriji ◽

10.15255/kui.2018.027 ◽

2019 ◽

Vol 68 (1-2) ◽

pp. 7-21

Author(s):

Modupe Ogunlesi ◽

Wesley Okiei ◽

Edith Ofor ◽

Anthony Eniola

Keyword(s):

Essential Oil ◽

Alternative Medicine ◽

Essential Oils ◽

Linolenic Acid ◽

Hexadecanoic Acid ◽

Ms Analysis ◽

Hexahydrofarnesyl Acetone ◽

Potential Applications

Adenia cissampeloides (Planch. ex Hook.) Harms is used in alternative medicine primarily for the management of hypertension and several other diseases. Isolation and GC-MS analysis of five essential oils collected fractionally and over 4 h by hydrodistillation from the dried leaves were carried out. The constituents included phytol, α-linolenic acid, n-hexadecanoic acid, hexahydrofarnesyl acetone, (13S)-8,13-epoxy-labd-14-ene, kaur-16-ene, guaiol, α-gurjunene, and α-elemene. Several bioactivities of the constituents as well as their potential applications in medicine and the pharmaceutical and pesticidal industries are discussed. Essential oils from some other plants have several constituents in common with those isolated from A. cissampeloides. The bioactivities of such plants are used to suggest other possible applications of A. cissampeloides.

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Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

Microorganisms ◽

10.3390/microorganisms10010109 ◽

2022 ◽

Vol 10 (1) ◽

pp. 109

Author(s):

Mohammadreza Pajohi Alamoti ◽

Behnaz Bazargani-Gilani ◽

Razzagh Mahmoudi ◽

Anna Reale ◽

Babak Pakbin ◽

...

Keyword(s):

Escherichia Coli ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Properties ◽

Multidrug Resistant ◽

Zataria Multiflora ◽

Cuminum Cyminum ◽

Plant Essential Oils ◽

E Coli ◽

Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

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CHEMICAL COMPOSITION, ADULTICIDAL AND REPELLENT ACTIVITY OF ESSENTIAL OILS FROM Mentha longifolia L. AND Lavandula dentata L. AGAINST Culex pipiens L.

Journal of Plant Protection and Pathology ◽

10.21608/jppp.2014.87995 ◽

2014 ◽

Vol 5 (7) ◽

pp. 817-826

Author(s):

Ali Al-Sarar

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Culex Pipiens ◽

Repellent Activity ◽

Mentha Longifolia

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Comparison of chemical compositions of essential oils isolated by hydrodistillation from wild thyme (Thymus serpyllum L.) with use of Deryng and Clevenger apparatus

Herba Polonica ◽

10.2478/hepo-2014-0006 ◽

2014 ◽

Vol 60 (2) ◽

pp. 7-17 ◽

Cited By ~ 4

Author(s):

Aneta Wesołowska ◽

Monika Grzeszczuk ◽

Dorota Jadczak

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Methyl Ether ◽

The Other ◽

Chemical Compositions ◽

Distillation Apparatus ◽

Ms Analysis ◽

Wild Thyme ◽

Thymus Serpyllum

Abstract The aim of the studies conducted in 2012-2013 was to compare the chemical composition of essential oils isolated from wild thyme (Thymus serpyllum L.) by hydrodistillation in Deryng and Clevenger apparatus. GC-MS analysis of the isolated oils revealed that carvacrol (42.81-45.24%), γ-terpinene (7.68-9.04%), β-caryophyllene (5.28-9.10%), β-bisabolene (5.76-6.91%) and carvacrol methyl ether (4.92-6.09%) were the major components of all the samples. On the basis of the obtained data it was proved that the type of distillation apparatus had no significant effect on the content of the main essential oil constituents of wild thyme. However, based on the means for both years of the study it was proved that hydrodistillation in Deryng apparatus was more effective for carvacrol concentration, while in Clevenger apparatus - for y-terpinene and carvacrol methyl ether concentration. The type of distillation apparatus had no significant effect on the content of the other essential oil constituents.

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