scholarly journals Vegetative propagation of Afzelia africana Sm. Ex Pers.: a multipurpose and threatened tree

2020 ◽  
Vol 14 (1) ◽  
pp. 204-212
Author(s):  
Ejeoghene Rita Ogbimi ◽  
Ayobola Moninuola Sakpere ◽  
Seun Michael Akinropo

Afzelia africana is at the verge of extinction from continuous exploitation without replacement, thus propagation of this tree species is expedient. Vegetative propagation is a reliable means of propagation of tree species. This study therefore describes a protocol for the vegetative propagation of Afzelia africana by using stem cuttings from its mature tree (20 years old) and saplings (2 years old). Herbaceous (non - lignified portion of the stem) and semi – hard wood cuttings (lignified portion of the stem) (with and without leaves) were treated with high and low concentrations of Indole butyric acid (IBA) solution via the quick dip and the soaking method of application and planted on forest soil and river sand in a humidifier. Cuttings from mature tree planted on forest soil and river sand did not respond to any treatment. Also, no response was observed on cuttings collected from saplings and planted on forest soil; however, cuttings planted on river sand sprouted. The presence and absence of leaves and IBA treatment had an interaction effect on root number (0.02) and root length (0.0001). The response from herbaceous cuttings was significantly higher than from semi – hard wood cuttings with respect to root number (1.00) and root length (5.82 cm). Cutting type and IBA treatment also had an interaction effect on root number (0.05) and root length (0.0007). Herbaceous leafy stem cuttings of A. africana treated with 400 ppm IBA and planted on river sand is suitable for rooting stem cuttings of A. africana for raising seedlings for reforestation, afforestation and other conservation purposes. © 2020 International Formulae Group. All rights reserved. Keywords: Rooting, cuttings, Afzelia africana, hormone, humidifier

1999 ◽  
Vol 17 (3) ◽  
pp. 123-125
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney

Abstract Shoot tip cuttings of Quercus phillyraeoides A. Gray ‘Emerald Sentinel’ (‘Emerald Sentinel’ oak), in a transitional growth stage between softwood and semi-hardwood, were collected from containerized plants growing under uniform fertility on June 4, 1998. Cuttings were treated with talc formulations of indolebutyric acid (IBA), ranging from 0 to 8000 ppm (0.8%), and placed under intermittent mist with shade levels excluding 0%, 30%, 60% or 90% of full solar irradiance. After 14 weeks, percent rooting averaged 18% and was unaffected by either IBA concentration or shading regime. Similarly, both mean root number per rooted cutting and mean root length were unaffected by IBA concentration or shading. Roots per rooted cutting and mean root length averaged 2.6 and 41 mm (1.6 in), respectively. Results suggest that shading stem cuttings of ‘Emerald Sentinel’ during propagation does not affect rooting.


HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 888D-888
Author(s):  
Muhammad Hatta ◽  
Caula A. Beyl ◽  
Stephen Garton

Trees of jujube (Ziziphus jujuba), particularly older ones, root with great difficulty. A greenhouse experiment was conducted to assess the effects of two strains of Agrobacterium rhizogenes (A4 and TR105) on softwood cuttings from two trees—a tree 10 years old not currently bearing flowers, which we called “juvenile” because it still exhibited many juvenile characteristics; and a tree ≈70 years old containing many flower buds, which we called “mature”. The cuttings were collected on 11 May 1994 and trimmed to 7.5 cm. Both strain and source of cutting influenced inoculation success—TR105 was more responsive to A. rhizogenes than was A4 and the “juvenile” cuttings more responsive than “mature” cuttings. Strain TR105 was very effective in increasing rooting percentages and root number. “Juvenile” cuttings had better rooting percentages, greater root number, and greater root length than did “mature” cuttings. Agrobacterium rhizogenes exhibits great potential for rooting other difficult woody ornamental or fruit tree species as well.


2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.


2018 ◽  
Vol 6 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Saumitro Das ◽  
L. K. Jha

Taxus baccata L., an endangered medicinal tree is highly valued for anti-cancer drug ‘Paclitaxel’ which is extracted from its leaves and barks. Reckless exploitation and habitat destruction have greatly reduced its natural population. Its regeneration through seed is also poor, so focus has been paid on vegetative propagation by stem cuttings to augment its natural regeneration. The present study was carried out to evaluate the effect of different rooting media on rooting behavior of Taxus baccata cuttings under poly house conditions. Effect of ten rooting media was studied on rooting of juvenile and mature stem cuttings of Taxus baccata. Six substrates i.e. field soil, forest soil, river sand, FYM, peat and vermi-compost were used for preparation of rooting media. The experiments were laid out in a randomized completely block design. The different rooting parameters were evaluated after six months of planting. The results revealed that the highest value of per cent survived, per cent callused, per cent rooted, mean number of root and mean root length was obtained in both juvenile and mature cuttings planted in Forest soil+ Peat+ FYM (1:1:1) compared to the other rooting media. Not a single cutting survived which was planted in river sand. Mature cuttings planted in Forest soil + River sand + VC (1:1:1) also failed to survive. The media containing peat as substrate significantly improved the rooting of cuttings but due to optimum physico-chemical properties, Forest soil+ Peat+ FYM (1:1:1) proved ideal rooting media for stem propagation of Taxus baccata.


Author(s):  
Ibrar Hussain ◽  
Abdul Samad ◽  
Nisar Naeem ◽  
Ziaullah ◽  
Hafeez ur Rehman ◽  
...  

Fig is a perennial fruit tree with high economic importance among horticultural crops in some countries. The fruit has a good market value, both at national and international level. The objective of this work was to evaluate the potential of vegetative propagation of fig cvs. ‘Sawari’ and ‘Tarnab Inzar’ through stem cuttings with different lengths, aiming to improve the production of nursery plants. The experiment was carried out from January to April 2017 and it was laid out in randomized complete block design with different sizes of cuttings (10, 15 and 20 cm) examined for two cultivars ‘Sawari’ and ‘Tarnab Inzar’, with three replications keeping 20 cuttings per treatment. The variables studied were number of leaves, number of roots, root length, shoot length, sprouting percentage and survival percentage. Cuttings size of fig cultivars had a significant temporal variation in the percentage of successful rooting and survival of cuttings. Based on data regarding number of leaves, number of roots, root length, sprouting percentage, it was concluded that 20 cm long cuttings collected for both cultivars are the best option for commercial production of fig nursery plants.


2014 ◽  
Vol 6 (2) ◽  
pp. 192-195
Author(s):  
Hamid HASSANPOUR ◽  
Mohamad ALI SHIRI

Clonal propagation of Cornelian cherry (Cornus mas L.) from cuttings will permit nursery production of cloned Cornelian cherry and encourage selection of superior genotypes. The effects of indole-3-butyric acid (IBA) concentrations, five genotypes and two types of media on root performance of Cornelian cherry cuttings were investigated. The percentage of rooted cuttings, mean root length, average root number per cutting, root fresh weight and root dry weight were recorded after three months. Orthogonal contrasts were used to test the effects of the auxin treatments. Regression analysis for determination of appropriate equation was performed. The IBA treatment increased the rooting percentage in genotype C1, C3 and C4 but did not affect that of genotype C2 and C5. The maximum rooting percentage, mean root length, average root number per cutting, root fresh weight and root dry weight was obtained for C3 genotype and in sand media. No statistically significant correlation was observed between callusing and other measured parameters but the rest of parameters correlate statistically with rooting. According to the regression results, it was clear that the pattern of quadratic function was better than other models for rooting. The highest rooting percentage was obtained from C3 genotype. Therefore, clonal production of this genotype is practical.


2017 ◽  
Vol 27 (1) ◽  
pp. 69-72
Author(s):  
Justin A. Schulze ◽  
Ryan N. Contreras ◽  
Carolyn F. Scagel

‘Schipkaensis’ common cherrylaurel (Prunus laurocerasus) is an important nursery crop across the United States. In our breeding efforts to reduce shot-hole symptoms and weediness, we have created chromosome doubled forms of this cultivar. Vegetative propagation is an important factor in nursery production, and we have found no studies that have looked at comparative adventitious rooting of stem cuttings using induced polyploids. The objective of this research was to determine if rooting ability varied between these two ploidy levels. Semihardwood stem cuttings from wild-type (22x) and polyploid (44x) ploidy levels were taken at the end of July 2015 and the beginning of July 2016. Cuttings were dipped in 1030 ppm (0.10%) indole-3-butyric acid (IBA) and 660 ppm (0.066%) 1-naphthaleneacetic acid (NAA) before being set in rooting substrate. After 1 month, cuttings were removed from substrate and data collected. Data included; rooting percentage, root number per rooted cutting, average root length, and total root length. In 2015, 88% of the cuttings from the 44x plants and 63% of the cuttings from the 22x plants rooted. In 2016, 100% of cuttings from both ploidy levels rooted. In both years, average root length and total root length were similar between ploidy levels; however, cuttings from 22x plants generally had more roots than those from 44x. Chromosome-doubled ‘Schipkaensis’ common cherrylaurel rooted effectively, and produce transplantable cuttings similar to the standard ploidy.


1998 ◽  
Vol 16 (4) ◽  
pp. 212-214
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney

Abstract Stem cuttings of Thuja L. x ‘Green Giant’ (‘Green Giant’ arborvitae), consisting of 22 cm (8.6 in) terminals (tips of first-order laterals) or 20 cm (7.8 in) laterals (side shoots removed from those portions of terminal cuttings inserted into the rooting medium), were collected on three dates associated with specific stock plant growth stages (softwood, semi-hardwood, and hardwood) and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm (0.9%). Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting for softwood, semi-hardwood, and hardwood, cuttings was 85%, 86%, and 96%, respectively. Semi-hardwood cuttings were the only cuttings in which percent rooting was affected by IBA treatment or cutting type, with lateral cuttings rooting in higher percentages than terminal cuttings (92% vs. 79%, respectively), while IBA concentrations of 3000 (0.3%) or 6000 ppm (0.6%) proved optimal for both cutting types. Mean root number and mean root length varied by growth stage, cutting type, and IBA concentration. In general, rooting percentages and root length were optimized at the hardwood stage with either cutting type treated with 3000, 6000 or 9000 ppm (0.3%, 0.6% or 0.9%) IBA, resulting in >10 roots per cutting.


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 875d-875
Author(s):  
C. N. Johnson ◽  
D. J. Eakes ◽  
L. L. Bruner ◽  
A. N. Wright ◽  
J. L Sibley

Clematis socialis Kral, also known as the Alabama Leatherflower, is an endangered species with only six known populations in northeast Alabama and northwest Georgia. Cutting propagation of the species would be beneficial for establishing additional self-sustaining populations and providing genetic material for future hybridization. A study conducted in 2000 and 2004 determined the effects of four nonamended substrates on root initiation and growth, as well as survival of C. socialis stem cuttings. Of the four substrates tested, including sand, perlite, vermiculite, and 1:1:1 (by volume) peat (P): pine bark (PB): sand (S), cutting survival was highest in sand in both 2000 and 2004. In 2000, sand also produced the longest roots and highest root quality. Vermiculite produced the longest and most roots and highest root quality in the 2004 study. In 2004, cuttings rooted in fine-particled substrates, such as sand and vermiculite, had higher cutting survival, root growth, root number, and root quality than those rooted in perlite and 1:1:1 (by volume) P:PB:S. The 1:1:1 P:PB:S substrate produced the lowest averages for all data collected in both the 2000 and 2004 studies. Sand was among the two highest performing media in both years, regardless of differences in IBA concentration, misting times, and environmental conditions, making it the overall best substrate for rooting C. socialis stem cuttings. Increasing the concentration of IBA in the rooting solution, providing a cooler environment, and decreasing the number and duration of misting cycles the cuttings received increased cutting survival, root length, root number, and root quality for all four substrates from 2000 to 2004.


2015 ◽  
Vol 33 (4) ◽  
pp. 155-159
Author(s):  
Justin A. Brock ◽  
Jason J. Griffin ◽  
Cheryl R. Boyer

Propagation substrates can strongly influence rooting success of stem cuttings. Eastern redcedar (Juniperus virginiana L.) chips (ERC) have been suggested as a propagation substrate component. This study investigated ERC as a perlite substitute in a perlite:sphagnum peat moss (3:1 v/v) rooting substrate. Stem cuttings of coleus [Solenostemon scutellarioides (L.) Codd], English ivy (Hedera helix L.), forsythia (Forsythia ×intermedia Zab.), lantana (Lantana camara L.), and spreading euonymus (Euonymus kiautschovicus Loes.) were rooted in substrates containing increasing concentrations of ERC hammer-milled to pass a 4.8 mm (0.19 in) screen. All species rooted well (≥ 95%) in all substrates except forsythia which rooted poorly in all substrates (8 to 36%). ERC concentration did not affect mean root number or mean root length in any species except spreading euonymus where mean root number peaked at 0 and 100% ERC content and mean root length decreased with increasing ERC content. Bulk density, container capacity, and total porosity increased as ERC replaced perlite. Physical properties of all substrates were suitable for cutting propagation. ERC can effectively replace perlite in rooting substrates for many ornamental species.


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