scholarly journals Effet de quelques légumineuses fourragères sur la reproduction, la croissance et la carcasse chez les cochons d’inde (Cavia porcellus L.)

2020 ◽  
Vol 14 (2) ◽  
pp. 600-612
Author(s):  
J. Gwladys Kontchiachou Nkana ◽  
Nathalie Ngouopo Mweugang ◽  
Soh Bah Germanus ◽  
Yam Alphonsius Semi ◽  
Paulette Ntsafack ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Total Mortality ◽  
Liver Weight ◽  
Rural Landscape ◽  
Calliandra Calothyrsus ◽  
Panicum Maximum ◽  
Cavia Porcellus ◽  
Arachis Glabrata ◽  
Significant Difference ◽  
Ad Libitum

Malgré l’importance de la caviaculture en milieu rural, la maîtrise de l’alimentation des cochons d’inde reste encore un handicap à cet élevage. C’est ainsi qu’entre octobre 2018 et mars 2019, un essai a été mené à la Ferme d’Application et de Recherche (FAR) de l’Université de Dschang en vue d’évaluer l’effet de quelques légumineuses fourragères comme source de protéine dans la ration sur les performances de production du cochon d’Inde. Pour cet essai, 56 cochons d’Inde de 5 mois d’âge de race locale pesant en moyenne 450 ± 50 g nés à la FAR ont été utilisés. En effet, 48 femelles repartis en quatre lots ont été mises en croisement avec 2 mâles par lot pendant une période de 31 jours selon un dispositif factoriel. Les animaux de chaque lot recevaient la graminée ad libitum associé à 20 g/j/animal de l’aliment composé contenant ou non une légumineuse. Les résultats montrent que les tailles de la portée sont restées comparables entre les traitements. Les taux de mortalité pré et post-sevrage (15,38% et 9,10%) ainsi que le poids des cochonnets à 8 semaines les plus élevés ont été enregistrés avec la ration contenant Arachis glabrata. A la naissance et au sevrage, le poids moyen le plus élevé des cochonnets a été obtenu avec la ration contenant Desmodium intortum. Les gains de poids des cochonnets sont restés comparables entre les traitements. Les rations contenant les légumineuses ont donné des rendements carcasses, le poids du foie et celui du cæcum significativement plus élevé. Compte tenu du coût de production, les légumineuses de cette étude peuvent être utilisées comme des sources de protéine de choix pour l'amélioration des performances de production des cobayes en milieu paysan.Mots clés : Arachis glabrata, Calliandra calothyrsus, Desmodium intortum, Panicum maximum, Performances de production.   Enlgish Title: Effect of some legumes forages on reproduction, growth and carcass of Guinea pigs (Cavia porcellus L.)Despite the importance of caviaculture in rural landscape, cavie’s feeding remains a handicap for this breeding. Thus, between October 2018 and March 2019, experiments were conducted at the Teaching and Experimental Farm of University of Dschang on the evaluation of some leguminaceous plants as protein source in the diet on production performances of Guinea pig. A total of 56 adults Guinea pigs of about 5 months and 450 ± 50 g were used for the experiments. Females were first put in breeding with 8 males for a period of 31 days and then organized in a completely randomized design of four groups of 12 females each. Each group received Panicum maximum ad libitum and 20 g DM/day/animal of a diet corresponding to the group. Mains results of this study showed that no significant difference (P > 0.05) has been noticed for mean litter size. Total mortality rate was 8.33 for diet containing Calliandra calothyrsus. No mortality was registered for others groups. High pre-weaning and post-weaning mortality (15.38 and 9.10%) was obtained with treatment based on Arachis glabrata. From birth to 8th week of age, no significant difference (P > 0.05) was observed between average live weights. Total and daily weight gains remain comparable (P > 0.05) in weaning and in post-weaning whatever the diet. Results for carcass evaluation showed that diet containing these plants as sources of protein has permitted to obtain the highest values. The same result was observed with the liver weight and cæcum. Considering the low production cost, legumes plants appear to be good protein sources for the improvement of production performances of the guinea pigs in small farmer environment. Keywords : Arachis glabrata, Calliandra calothyrsus, Desmodium intortum, Panicum maximum, production performances.

scholarly journals Carcass and organ characteristics of finishing broilers fed diets containing probiotics (Saccharomyces cerevisiae)

Agro-Science ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 25-30
Author(s):  
J.I. Ugwuoke ◽  
O.R. Okwesili ◽  
C.E. Dim ◽  
M.N. Okonkwo ◽  
H.M. Ndofor-Foleng
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Performance ◽  
Liver Weight ◽  
Heart Weight ◽  
Performance Indices ◽  
Randomized Design ◽  
Significant Difference ◽  
Ad Libitum ◽  
Completely Randomized Design ◽  
Effect Of Feeding

A five-week study was conducted to determine the effect of feeding varying levels of S. cerevisiae on carcass and organ characteristics of finishing broilers. One hundred and twenty 4-weeks old broilers of cobb strain were randomly assigned to four treatments (T1 = 0.6 g Saccharomyces cerevisiae (SC) kg–1 diet; T2 = 0.8 g SC kg–1 diet; T3 = 1.0 g SC kg–1 diet and T4 = 0.0 g SC kg–1 diet) with 30 birds per treatment and replicated twice with 15 birds per replicate in a completely randomized design. Feed and water were provided ad libitum to the birds in a deep litter system. In the end, data on growth, carcass and organ indices generated from the study were analyzed using one-way analysis of variance (ANOVA). Results showed no significant difference (p > 0.05) among the treatments in the birds’ growth performance indices. However, significant differences (p < 0.05) were observed in the birds’ values for liver weight, heart weight, shank length and thigh length with birds in T3 recording highest values of 61.30 g, 16.93 g and 12.00 cm for liver weight, heart weight and thigh length, respectively. It was thus concluded that finishing broilers fed 1.0 g of S. cerevisiae had superior carcass and organ characteristics than birds on the control and lower levels of inclusion.

scholarly journals Krim ekstrak kulit delima merah (Punica granatum) menghambat peningkatan jumlah melanin sama efektifnya dengan krim hidrokuinon pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Eva R. Siahaan ◽  
Wimpie Pangkahila ◽  
A A G P Wiraguna
Keyword(s):  
Guinea Pigs ◽  
Punica Granatum ◽  
Control Group ◽  
Cavia Porcellus ◽  
Pomegranate Peel ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract: As the outer organ of the body, skin becomes the main target of environmental influences, particularly the UVB rays. Based on analysis of phytochemicals, red pomegranate peel extract contains antioxidants such as flavonoids, phenols, tannins, and vitamin C which are able to prevent increased skin melanin by inhibiting the actions of tyrosinase. This study was aimed to prove that application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in female guinea pigs (Cavia porcellus) exposed to UVB as well as to prove that the 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin. This was a true experimental study using post test only control group design. Subjects were divided into three groups, with 10 guinea pigs each group. Group 1, the control group, was treated with UVB exposure and basic cream. Group 2 was treated with UVB exposure and 4% hydroquinone cream. Group 3 was treated with UVB and 8% red pomegranate peel extract cream. A total of 390 mJ/cm UVB dosage was given for 2 weeks. Histopahtologic slides were stained with Masson-Fontana. The amount of melanin area colored in black was evaluated by the percentage of pixel areas of melanin compared with pixel areas of all epidermal tissues. The results showed that the highest amount of melanin was in group 1 (18.13±4.76%), followed by group 3 (1.876±0.73%), and group 2 as the lowest (0.67±0.291%). There was a significant difference between control group and group 2 as s well as group 3 (P <0.05). There was no significant difference between group 2 and group 3 (P >0.05). Conclusion: Application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in guinea pigs (Cavia porcellus) exposed to UVB. The 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin.Keywords: red pomegranate peel extract, melanin, skin, UVBAbstrak: Sebagai organ terluar dari tubuh, kulit menjadi target utama dari pengaruh lingkungan, terutama oleh sinar UVB. Berdasarkan analisis fitokimia, ekstrak kulit delima merah memiliki kandungan antioksidan seperti flavonoid fenol, tanin, dan vitamin C yang mampu mencegah peningkatan jumlah melanin dengan cara menghambat kerja enzim tirosinase. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak kulit delima merah (Punica granatum) 8% dalam menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB dan krim ekstrak kulit delima merah (Punica granatum) 8% memiliki efektivitas yang sama dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin tersebut. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian dibagi menjadi tiga kelompok, masing-masing berjumlah 10 ekor marmut betina. Kelompok 1 (kontrol) diberikan pajanan sinar UVB dan krim dasar; kelompok 2 diberikan paparan sinar UVB dan krim hidrokuinon 4%; dan kelompok 3diberikan pajanan sinar UVB dan krim ekstrak kulit delima merah 8%. Dosis total UVB 390 mJ/cm2 yang diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit menggunakan pewarnaan Masson-Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan bahwa jumlah melanin paling tinggi terdapat pada kelompok 1 (18,13±4,76%), diikuti kelompok 3 (1,876±0,73%), dan paling rendah pada kelompok 2 (0,67±0,291%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05) sedangkan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak kulit delima merah (Punica granatum) 8% dapat menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina. Krim ekstrak kulit delima merah (Punica granatum) 8% sama efektif dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin pada kulit marmut.Kata kunci: kulit delima merah, melanin, kulit, UVB, marmut betina.

scholarly journals Comparative Growth Performance in Post-Weaned Guinea Pigs (Cavia Porcellus L) Fed with Panicum Maximum or Pennisetum Purpureum

2018 ◽  
Vol 6 (6) ◽  
Author(s):  
Emile Miégoué ◽  
Fernand Tendonkeng ◽  
Jules Lemofouet ◽  
Paulette Ntsafack ◽  
Nathalie Mweugang Ngouopo ◽  
...  
Keyword(s):  
Growth Performance ◽  
Guinea Pigs ◽  
Pennisetum Purpureum ◽  
Panicum Maximum ◽  
Cavia Porcellus

scholarly journals Physiological Features of Pennisetum Purpureum or Panicum Maximum Consumption in Guinea Pigs (Cavia Porcellus)

2018 ◽  
Vol 1 (1) ◽  
pp. 5-19
Author(s):  
Emile Miégoué ◽  
Fernand Tendonkeng ◽  
Nathalie Mweugang Ngouopo ◽  
Loïc Arnauld Mba Tene ◽  
Paulette Ntsafack ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Pennisetum Purpureum ◽  
Average Weight ◽  
Panicum Maximum ◽  
Cavia Porcellus ◽  
Fresh Matter ◽  
Local Breed ◽  
The University ◽  
Better Than

Guinea pig diet is essentially based on the use of grasses associated with protein sources. Then, in to improve the herbivores nutrition, the evaluation of intake and In vivo digestibility of Pennisetum purpureum or Panicum maximum in guinea pigs (Cavia porcellus) was carried out in January 2017 at the University of Dschang research and experimental farm and animal production and nutrition laboratory. 20 adult animals of local breed on average weight 450 ± 50g and aged about 5 months were used. They were organized into two batches of 10 animals each (5 males and 5 females). The first batch received 250g of fresh matter of Pennisetum purpureum + 60g concentrate/animal/day, while the second received the same treatment with Panicum maximum as grasses. Leftover and animals were weighed every morning before food distributions, and drinking water containing vitamin C was served ad libitum. According to the results, the highest ingestion was obtained with Panicum maximum. However, the digestibility different nutrients was comparable between treatments; nevertheless, males fed on P. maximum digested crude cellulose (63.78%) better than males fed on P. purpureum (51.17%). With regard to the variation of the bacterial rate of the caecal flora, enterobacteria of animals fed P. maximum was high (11.20 CFU/ml) compared to those of the animals receiving P. purpureum (7.27 CFU/ml), although regardless of the treatment, the level of lactobacilli was higher than that of enterobacteria. In view of the results obtained, these grasses can be alternatively used in feeding guinea pigs.

scholarly journals Palatabilité de onze fourrages tropicaux chez des cobayes (Cavia porcellus) de différents stades physiologiques au B

2019 ◽  
Vol 41.2 ◽  
pp. 6916-6925
Author(s):  
Alex Ghêliho Zoffoun ◽  
Abossèdé Murielle Lucrèce Faïhun
Keyword(s):  
Guinea Pigs ◽  
Elaeis Guineensis ◽  
Musa Acuminata ◽  
Pennisetum Purpureum ◽  
P Value ◽  
Panicum Maximum ◽  
Cavia Porcellus ◽  
Tridax Procumbens ◽  
Forage Species ◽  
Guinea Grass

Évaluer la palatabilité de onze fourrages tropicaux chez les cobayes (Cavia porcellus). Des cobayes appartenant à deux stades physiologiques (jeunes et adultes) ont fait l’objet des tests sur la palatabilité. Onze espèces fourragères tirées des résultats d’enquête auprès des éleveurs locaux ont été utilisées : Panicum maximum C1(Herbe de Guinée var C1), Panicum maximum local (Herbe de Guinée local), Pennisetum purpureum (Herbe éléphant), Elaeis guineensis (Palmier à huile), Musa acuminata (Bananier), Manihot esculenta (Manioc), Carica papaya (Papayer), Centrosema pubescens (Haricot sauvage), Moringa olifera (Moringe), Talinum triangulare (Pourpier tropical) et Tridax procumbens (Herbe à lapins). L’indice de palatabilité (IP) de chaque fourrage a été déterminé selon la méthode décrite par Salem et al. (2000). Panicum maximum local a présenté l’indice de préférence le plus élevé aussi bien chez les jeunes que chez les adultes, les valeurs ont été respectivement de 0,22 et 0,38. Centrosoma pubescens, Tridax procumbens et Panicum maximum C1 ont aussi présenté des indices de préférence élevés pour les deux stades physiologiques des cobayes. Les valeurs obtenues pour Centrosoma pubescens ont été de 0,22 chez les jeunes contre 0,25 chez les adultes, pour Tridax procumbens 0,19 chez les jeunes contre 0,24 chez les adultes, pour Panicum maximum C1 0,10 chez les jeunes contre 0,15 chez les adultes. Les valeurs d’indice de préférence les plus faibles ont été obtenues pour les espèces fourragères Musa acuminata (IP=0,00 chez les jeunes contre 0,02 chez les adultes) et Elaeis guineensis (IP=0,01 chez les jeunes contre 0,01 chez les adultes). Les valeurs obtenues pour les indices de préférences ont été influencées significativement par l’âge de l’animal (p-value = 0.005651) et le type de fourrage (p-value < 2.2e-16). La présente étude a permis d’évaluer la palatabilité de onze espèces fourragères chez des cobayes de différents stades physiologiques et d’en retenir les plus appétées par ces animaux. La connaissance des espèces fourragères les plus appréciées par les cobayes et la composition chimique de ces dernières permettront d'optimiser les stratégies d'alimentation dans les élevages. Palatability of eleven tropical forages in guinea pigs (Cavia porcellus) of different physiological stages in Benin To evaluate the palatability of eleven tropical forages in guinea pigs (Cavia porcellus). Guinea pigs from two physiological stages (young and adult) were tested for palatability. Eleven forage species from survey results from local breeders were used : Panicum maximum C1 (Guinea grass C1), Panicum maximum local (local Guinea grass), Pennisetum purpureum (Elephant grass), Elaeis guineensis (African oil palm), Musa acuminata (Banana), Manihot esculenta (Cassava), Carica papaya (Pawpaw), Centrosema pubescens (Butterfly pea), Moringa olifera (Moringa), Talinum triangulare (Potherb fameflower) et Tridax procumbens (Coat buttons). The palatability index (PI) of each forage was determined by following the procedure described by Salem et al. (2000). Local Panicum maximum had the highest preference index in both young and adults, with values of 0.22 and 0.38, respectively. Centrosoma pubescens, Tridax procumbens and Panicum maximum C1 also showed high preference indices for the two physiological stages of guinea pigs. The values obtained for Centrosoma pubescens were 0.22 in the young against 0.25 in the adults, for Tridax procumbens 0.19 in the young against 0.24 in the adults, for Panicum maximum C1, 0.10 in the young against 0.15 in adults. The lowest preference index values were obtained for the forage species Musa acuminata (IP = 0.00 in young versus 0.02 in adults) and Elaeis guineensis (IP = 0.01 in young versus 0.01 in adults). The values obtained for the preference indices were significantly influenced by the age of the animal (p-value = 0.005651) and the type of forage (p-value <2.2e-16). The present study evaluated the palatability of eleven forage species in guinea pigs of different physiological stages and retained the most palatable of these animals. The knowledge of the forage species most appreciated by the guinea pigs and the chemical composition of the latter will optimize the feeding strategies in the farms.

scholarly journals Croissance pré-sevrage des cobayes nourris au Panicum maximum supplémenté avec une ration contenant Arachis glabrata, Calliandra calothyrsus ou Desmodium intortum

2016 ◽  
Vol 10 (1) ◽  
pp. 313 ◽  
Author(s):  
Emile Miegoue ◽  
Fernand Tendonkeng ◽  
Jules Lemoufouet ◽  
Marie Noel Bertine Noumbissi ◽  
Ngouopo Nathalie Mweugang ◽  
...  
Keyword(s):  
Calliandra Calothyrsus ◽  
Panicum Maximum ◽  
Arachis Glabrata

scholarly journals Effect of antioxidant supplements on testicular histo-morphology in adult male guinea pigs (Cavia porcellus)

2020 ◽  
Vol 47 (5) ◽  
pp. 29-40
Author(s):  
S. Gaddafi ◽  
M. G. Garba ◽  
M. Abdulrashid ◽  
D. Zahradden ◽  
O. M. Daudou ◽  
...  
Keyword(s):  
Vitamin C ◽  
Adult Male ◽  
Guinea Pigs ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Cavia Porcellus ◽  
Left Testis ◽  
Vitamine C ◽  
Ad Libitum ◽  
Antioxidant Supplements

The study was carried out to evaluate the biopotency of antioxidant supplements on physiological, testicular and oxidative biomarkers in adult male guinea pigs (Cavia porcellus). A total of 60 adult male guinea pigs of three months of age with an average body weight of 500±20g were randomly allocated into three dietary treatment groups of vitamin C, vitamin E and selenium and each comprised four varying levels with five animals per group in a completely randomized design (CRD). Feed and water were provided ad-libitum. The testicular morphology showed a significant (P<0.05) difference in weight of right testis, weight of left testis, paired testis weight, right testis width, testis volume, testis density, right testis length and left testis length of guinea pig supplemented varying dosage of vitamin C, E and Selenium. microphotograph indicated considerable changes in seminiferous tubules density, size, and other morphological characteristics, also intertubular space, basement membrane, leydigs cells and spermatogonia of the guinea pigs supplemented varying dosage of vitamin C, E and Selenium revealed considerable changes. The supplementation of adult male guinea pigs diets with 200 mg vitamin C had the highest values of testicular morphometric followed by 15 mg vitamin E group and 0.3 mg of selenium. Similarly, considerable histo-architectural changes in seminiferous tubules and interstitial cells size and shape occurred in guinea pigs supplemented 200, 15 and 0.3 mg per kg diet vitamin C, E and selenium respectively. Therefore, supplementation of vitamin C, E and Selenium has a profound effect on testicular histo-morphology of adult male guinea pigs.     L'étude a été réalisée pour évaluer la biopotence des suppléments antioxydants sur les biomarqueurs physiologiques, testiculaires et oxydatifs chez les cobayes mâles adultes (Caviaporcellus). Un total de 60 cobayes mâles adultes de trois mois avec un poids corporel moyen de 500 ± 20 g ont été répartis au hasard dans trois groupes de traitement diététique de vitamine C, vitamine E et sélénium et chacun comprenait quatre niveaux variables avec cinq animaux par groupe en une conception complètement aléatoire. L'alimentation et l'eau étaient fournies ad libitum. La morphologie testiculaire a montré une différence significative (P <0.05) du poids du testicule droit, du poids du testicule gauche, du poids du testicule apparié, de la largeur du testicule droit, du volume du testicule, de la densité du testicule, de la longueur du testicule droit et de la longueur du testicule gauche du cobaye supplémenté en doses variables de vitamine C, E et sélénium. La microphotographie a indiqué des changements considérables dans la densité, la taille et d'autres caractéristiques morphologiques des tubules séminifères, ainsi que l'espace intertubulaire, la membrane basale, les cellules de 'leydigs' et la spermatogonie des cobayes complétés par des doses variables de vitamine C, E et de sélénium ont révélé des changements considérables. La supplémentation des régimes alimentaires de cobayes mâles adultes avec 200 mg de vitamine C avait les valeurs les plus élevées de morphométrie testiculaire suivie par 15 mg de groupe de vitamine E et 0.3 mg de sélénium. De même, des changements histoarchitecturaux considérables de la taille et de la forme des tubules séminifères et des cellules interstitielles se sont produits chez les cobayes ayant reçu respectivement 200, 15 et 0.3 mg par kg de vitamine C, E et de sélénium. Par conséquent, la supplémentation en vitamine C, E et en sélénium a un effet profond sur l'histomorphologie testiculaire des cobayes males adultes.

scholarly journals Krim ekstrak etanol biji mengkudu (Morinda citrifolia) sama efektifnya dengan krim hidrokuinon dalam mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar ultraviolet B

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Rahmi Sofiana ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Guinea Pigs ◽  
Ultraviolet B ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Cavia Porcellus ◽  
Control Group Design ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract: Ultraviolet B (UVB) ray may cause skin hyperpigmentation due to increased melanin level. Noni seeds (Morinda citrifolia) extract was a strong candidate as an antioxidant and whitening agent. This study was aimed to prove the effect of noni seeds extract cream in prevention the increase of melanin in UVB-exposed guinea pig (Cavia porcellus). This was a true experimental study using posttest only control group design. Subjects were 30 guinea pigs divided into 3 groups, each of 10 guinea pigs. Group 1, the control group, was treated with UVB exposure and basic cream; group 2 was treated with UVB and 4% noni seed cream; and group 3 was treated with UVB and 4% hydroquinone cream. A total of 390 mJ/cm2 ultraviolet B dosage was given for 2 weeks. Histopathological examination with Masson-Fontana staining was performed to evaluate the melanin areas with black color. The amount of melanin was calculated by the percentage of pixel areas of melanin and was compared with the pixels of all epidermal tissues. The results showed that the highest number of melanin was in group 1 (10.61±5.33%), while in group 2 was 1.4±0.65%, and in group 3 was 0.45±0.23%. There was a significant difference between the control group and group 2 as well as group 3 (P <0.05) whereas no significant difference was found between group 2 and group 3 (P >0.05). Conclusion: The 4% noni seeds extract (Morinda citrifolia) cream could prevent the increase of skin melanin in UVB-exposed guinea pig as effective as 4% hydroquinone cream.Keywords: noni seeds extract cream, melanin, ultraviolet B, guinea pigs Abstrak: Paparan sinar ultraviolet B (UVB) mengakibatkan terjadinya kelainan hiperpigmentasi yang ditandai dengan peningkatan jumlah melanin. Ekstrak biji mengkudu memiliki kombinasi zat aktif yang dapat bekerja sinergis dalam mencegah peningkatan jumlah melanin. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak biji mengkudu (Morinda citrifolia) dalam mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar UVB. Jenis penelitian ialah eksperimental dengan randomized posttest only control group design menggunakan 30 ekor marmut jantan yang dibagi atas 3 kelompok, masing-masing 10 ekor. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan krim ekstrak biji mengkudu 4%. Kelompok 3 diberi paparan sinar UVB dan krim hidrokuinon 4%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Pemeriksaan histopatologik jaringan kulit dengan pewarnaan Masson- Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin pada Kelompok 1 sebesar 10,61±5,33%; pada kelompok 2 sebesar 1.4±0.65%, dan pada kelompok 3 sebesar 0,45±0,23%. Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Perbandingan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak biji mengkudu 4% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB, dan memiliki efektifitas yang sama dengan krim hidrokuinon 4%. Kata kunci: krim ekstrak biji mengkudu, melanin, ultraviolet B, marmut

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