scholarly journals Hepatocurative potentials of methanol extracts/fractions of Tapinanthus bangwensis and Moringa oleifera on carbon tetrachlorideinduced hepatotoxicity in Wistar rats

2020 ◽  
Vol 22 (2) ◽  
pp. 001-014
Author(s):  
G.O. Ihegboro ◽  
A.J. Alhassan ◽  
E. Afor ◽  
C.J. Ononamadu ◽  
T.A. Owolarafe ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Mineral Elements ◽  
Toxic Substances ◽  
Methanol Extracts ◽  
Lipid Parameters

Bioaccumulation of toxic substances in liver poses a serious threat to its functionality and consequently leads to damage/injury. Medicinal plants have been reported to be effective in the treatment and management of various diseases. The present study is aimed at evaluating the hepatocurative potentials of Tapinanthus bangwensis and Moringa oleifera on carbon tetrachloride-induced hepatotoxicity in Wistar rats. Atomic absorption spectroscopy (AAS) and ultraviolet-visible spectrophotometry were respectively used to assay for mineral elements and antioxidant activity of methanol extracts/fractions of T. bangwensis and M. oleifera. Concentrations of potassium in methanol extract of M. oleifera (MeCE 2) and magnesium in T. bangwensis (MeCE 1) were found to be significantly high (p < 0.05). Concentrations of trace elements were low in the range of 0.07 mg/kg to 0.60 mg/kg. The in vitro lipid peroxidation assay showed that ethylacetate fraction and acetone fraction of T. bangwensis (ETF 1 and ACF 1) respectively exhibited the strongest and lowest antioxidant activity compared to other extracts/fractions. Oral administration of methanol extracts/fractions of T. bangwensis and M. oleifera significantly increased superoxide dismutase (SOD) and catalase (CAT) activities, increased glutathione (GSH) level and decreased malondialdehyde (MDA) level. The liver function indices (or liver biomarkers) such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin, total protein, total and conjugated bilirubin and lipid parameters (low density lipoprotein (LDL-c), cholesterol and triglyceride) were also significantly reduced. However high density lipoprotein (HDL-c) increased in the groups induced and treated with methanol extracts/fractions of T. bangwensis and M. oleifera compared to the group induced but untreated. It can be concluded that the plants possessed hepatocurative potential against CCl - 4 induced hepatotoxicity in Wistar rats Keywords: Bioaccumulation, Hepatotoxicity, Liver biomarkers, Moringa oleifera Tapinanthus bangwensis

scholarly journals Influence of Honey on the Suppression of Human Low Density Lipoprotein (LDL) Peroxidation (In Vitro)

2009 ◽  
Vol 6 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Ahmed G. Hegazi ◽  
Faten K. Abd El-Hady
Keyword(s):  
Antioxidant Activity ◽  
Hplc Analysis ◽  
Low Density Lipoprotein ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Free Radical Scavenging ◽  
Low Density ◽  
Dpph Assay ◽  
Free Radical Scavenging Assay

The antioxidant activity of four honey samples from different floral sources (Acacia, Coriander, Sider and Palm) were evaluated with three different assays; DPPH free radical scavenging assay, superoxide anion generated in xanthine–xanthine oxidase (XOD) system and low density lipoprotein (LDL) peroxidation assay. The dark Palm and Sider honeys had the highest antioxidant activity in the DPPH assay. But all the honey samples exhibited more or less the same highly significant antioxidant activity within the concentration of 1mg honey/1 ml in XOD system and LDL peroxidation assays. The chemical composition of these samples was investigated by GC/MS and HPLC analysis, 11 compounds being new to honey. The GC/MS revealed the presence of 90 compounds, mainly aliphatic acids (37 compounds), which represent 54.73, 8.72, 22.87 and 64.10% and phenolic acids (15 compound) 2.3, 1.02, 2.07 and 11.68% for Acacia, Coriander, Sider and Palm honeys. In HPLC analysis, 19 flavonoids were identified. Coriander and Sider honeys were characterized by the presence of large amounts of flavonoids.

scholarly journals Correlation between Chemical Composition ofCurcuma domesticaandCurcuma xanthorrhizaand Their Antioxidant Effect on Human Low-Density Lipoprotein Oxidation

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Ibrahim Jantan ◽  
Fadlina Chany Saputri ◽  
Muhammad Naeem Qaisar ◽  
Fhataheya Buang
Keyword(s):  
Antioxidant Activity ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Thiobarbituric Acid ◽  
Low Density ◽  
Thiobarbituric Acid Reactive Substances ◽  
Lipoprotein Oxidation ◽  
Strong Activity ◽  
Methanol Extracts ◽  
Mediated Oxidation

The antioxidant activity of the curcuminoids ofCurcuma domesticaL. andC. xanthorrhizaRoxb. and eight compounds which are prevalent constituents of their rhizome oils were investigated in an effort to correlate human low-density lipoprotein (LDL) antioxidant activity with the effect of the herbs and their components. The antioxidant activity was examined using thiobarbituric acid reactive substances (TBARSs) assay with human LDL as the oxidation substrate. The methanol extracts and rhizome oils ofC. xanthorrhizaandC. domesticashowed strong inhibitory activity on copper-mediated oxidation of LDL. Curcumin, demethoxycurcumin, and bisdemethoxycurcumin, isolated from the methanol extracts of both plants, exhibited stronger activity than probucol (IC50value 0.57 μmol/L) as reference, with IC50values ranging from 0.15 to 0.33 μmol/L. Xanthorrhizol, the most abundant component (31.9%) of the oil ofC. xanthorrhiza, showed relatively strong activity with an IC50value of 1.93 μmol/L. The major components ofC. domestica, ar-turmerone (45.8%) and zerumbone (3.5%), exhibited IC50values of 10.18 and 24.90 μmol/L, respectively. The high levels of curcuminoids in the methanol extracts and xanthorrhizol, ar-turmerone and zerumbone in the oils, and in combination with the minor components were responsible for the high LDL antioxidant activity of the herbs.

scholarly journals Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties

1998 ◽  
Vol 330 (3) ◽  
pp. 1173-1178 ◽  
Author(s):  
E. Jonathan BROWN ◽  
Hicham KHODR ◽  
C. Robert HIDER ◽  
Catherine A. RICE-EVANS
Keyword(s):  
Antioxidant Activity ◽  
Complex Formation ◽  
Hydroxy Group ◽  
Low Density Lipoprotein ◽  
Antioxidant Properties ◽  
Density Lipoprotein ◽  
Low Density ◽  
Structural Dependence ◽  
Chelate Formation

The flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties in vitro. The interactions of four structurally related flavonoids (quercetin, kaempferol, rutin and luteolin) with Cu2+ ions were investigated in terms of the extent to which they undergo complex formation through chelation or modification through oxidation, as well as in their structural dependence. The ortho 3ʹ,4ʹ-dihydroxy substitution in the B ring is shown to be important for Cu2+-chelate formation, thereby influencing the antioxidant activity. The presence of a 3-hydroxy group in the flavonoid structure enhances the oxidation of quercetin and kaempferol, whereas luteolin and rutin, each lacking the 3-hydroxy group, do not oxidize as readily in the presence of Cu2+ ions. The results also demonstrate that the reactivities of the flavonoids in protecting low-density lipoprotein (LDL) against Cu2+ ion-induced oxidation are dependent on their structural properties in terms of the response of the particular flavonoid to Cu2+ ions, whether chelation or oxidation, their partitioning abilities between the aqueous compartment and the lipophilic environment within the LDL particle, and their hydrogen-donating antioxidant properties.

Therapeutic Application of Diacylglycerol Oil for Obesity: Serotonin Hypothesis

2012 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Hidekatsu Yanai ◽  
Hiroshi Yoshida ◽  
Yuji Hirowatari ◽  
Norio Tada
Keyword(s):  
Energy Expenditure ◽  
Molecular Mechanisms ◽  
Uncoupling Protein ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serotonin Release ◽  
Intestinal Cell ◽  
Therapeutic Application ◽  
Postprandial Triglyceride

Characteristics for the serum lipid abnormalities in the obesity/metabolic syndrome are elevated fasting, postprandial triglyceride (TG), and decreased high-density lipoprotein-cholesterol (HDL-C). Diacylglycerol (DAG) oil ingestion has been reported to ameliorate postprandial hyperlipidemia and prevent obesity by increasing energy expenditure, due to the intestinal physiochemical dynamics that differ from triacylglycerol (TAG). Our study demonstrated that DAG suppresses postprandial increase in TG-rich lipoprotein, very low-density lipoprotein (VLDL), and insulin, as compared with TAG in young, healthy individuals. Interestingly, our study also presented that DAG significantly increases plasma serotonin, which is mostly present in the intestine, and mediates thermogenesis, proposing a possible mechanism for a postprandial increase in energy expenditure by DAG. Our other study demonstrated that DAG suppresses postprandial increase in TG, VLDL-C, and remnant-like particle-cholesterol, in comparison with TAG in an apolipoprotein C-II deficient subject, suggesting that DAG suppresses postprandial TG-rich lipoprotein independently of lipoprotein lipase. Further, to understand the molecular mechanisms for DAG-mediated increase in serotonin and energy expenditure, we studied the effects of 1-monoacylglycerol and 2-monoacylglycerol, distinct digestive products of DAG and TAG, respectively, on serotonin release from the Caco-2 cells, the human intestinal cell line. We also studied effects of 1- and 2-monoacylglycerol, and serotonin on the expression of mRNA associated with β-oxidation, fatty acids metabolism, and thermogenesis, in the Caco-2 cells. 1-monoacylglycerol significantly increased serotonin release from the Caco-2 cells, compared with 2-monoacylglycerol by approximately 40%. The expression of mRNA of acyl-CoA oxidase (ACO), fatty acid translocase (FAT), and uncoupling protein-2 (UCP-2), was significantly higher in 1-MOG-treated Caco-2 cells, than 2-MOG-treated cells. The expression of mRNA of ACO, medium-chain acyl-CoA dehydrogenase, FAT, and UCP-2, was significantly elevated in serotonin-treated Caco-2 cells, compared to cells incubated without serotonin. In conclusion, our clinical and in vitro studies suggested a possible therapeutic application of DAG for obesity, and obesity-related metabolic disorders.Key words: Diacylglycerol, intestine, obesity, serotonin, thermogenesis

Antidyslipidemic and Antioxidant Activities of Matricaria pubescens (Desf.) Shultz.in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 18 ◽  
Author(s):  
Ayoub Amssayef ◽  
Bouchra Azzaou ◽  
Mohammed Ajebli ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Favorable Effect ◽  
Lipoprotein Profile ◽  
Phytochemical Compounds ◽  
Asteraceae Family

Aims: The study aimed to evaluate the antihyperlipidemic and antioxidant activities of Matricaria pubescens. Background: Matricaria pubescens (Desf.) Shultz belongs to Asteraceae family and it is commonly used traditionally for handling diabetes mellitus. Objective: The objective of this study was to assess the antioxidant activity of Matricaria pubescens (Desf.) Shultz and its effect on lipid and lipoprotein profile in normal and streptozotocin-induced diabetic rats. Methods: The effect of repeated (7 days of treatment) oral administration of the aqueous extract of aerial part of Matricaria pubescens (MPAE) at a dose of 40 mg/kg on lipid and lipoprotein profile was examined in normal and streptozotocin-induced diabetic rats. Furthermore, a preliminary phytochemical screening and the quantification of phenolic, flavonoid and tannin contents as well as the antioxidant activity using two methods (FRAP and ABTS) were carried out. Results: MPAE demonstrated a potent antidyslipidemic effect in diabetic rats by reducing serum levels of triglycerides, total cholesterol and low-density lipoprotein (LDL). In addition, the results showed that the extract is rich in several phytochemical compounds and revealed an important antioxidant activity. Conclusion: In summary, this study proved that Matricaria pubescens (Desf.) Shultz. has a favorable effect on diabetic dyslipidemia.

Study of Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Tannins Extracted from Warionia saharae Benth. & Coss

2019 ◽  
Vol 19 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Mohammed Ajebli ◽  
Fadwa El Ouady ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Soxhlet Apparatus

Background and Objective: Warionia saharae Benth & Coss, a plant belonging to Asteraceae family, is used for its anti-diabetic properties in Morocco. The objective of this study was to evaluate the effect of tannins extracted from Warionia saharae (W. saharae) on blood glucose levels and lipid profile in normal and streptozotocin(STZ)-induced diabetic rats. Methods: Tannins (TE) were extracted from W. saharae using Soxhlet apparatus and different organic solvents. Single and once daily repeated oral administration of TE (10 mg/kg) for 15 days were used to evaluate the glucose and lipid-lowering activity in normal and diabetic rats. Furthermore, glucose test tolerance, liver histopathological examination and in vitro antioxidant activity of TE were carried out in this study. Results: The results showed that TE was able to exert antihyperglycemic and lowering total cholesterol effects as well as improvement of the high-density lipoprotein (HDL)-cholesterol serum level after 15 days of treatment. Furthermore, TE improved glucose tolerance, histopathological status of liver in diabetic rats and demonstrated interesting antioxidant activity. Conclusion: In conclusion, the present investigation revealed that TE possesses potent antidiabetic and antihyperlipidemic activities as claimed in different ethnopharmacological practices.

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