scholarly journals Antimicrobial activity of Moringa oleifera leaf extracts on multiple drug resistant bacterial isolates from urine samples in Benin City

2019 ◽  
Vol 35 (2) ◽  
pp. 16
Author(s):  
O.A. Eremwanarue ◽  
H.O. Shittu
Keyword(s):  
Antimicrobial Activity ◽  
Moringa Oleifera ◽  
Urine Samples ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Bacterial Isolates ◽  
Leaf Extracts ◽  
Benin City ◽  
Multiple Drug Resistant

scholarly journals Multiple drug resistance in bacterial isolates from liquid wastes generated in central hospitals of Nepal

1970 ◽  
Vol 8 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Dr Sharma ◽  
B Pradhan ◽  
SK Mishra
Keyword(s):  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Liquid Waste ◽  
Viable Count ◽  
Plate Count ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Bacterial Isolates ◽  
Multiple Drug Resistant ◽  
Liquid Wastes

Background: Healthcare liquid wastes are the reservoirs of harmful infectious agents such as the pathogens and multiple drug resistant microorganisms. Potential infectious risks include the spread of infectious diseases and microbial resistance from health-care establishments into the environment and thereby posing risks of getting infections and antibiotic resistance in the communities. Objectives: The objectives of this study were to assess the bacterial load of healthcare liquid waste generated in central hospitals and to explore the antimicrobial resistance pattern of these bacterial isolates. Materials and methods: A descriptive study was carried out in 10 conveniently selected central hospitals of Nepal during the period of May to December 2008. Effluent specimens from each hospital were subjected to total viable count studies by spread plate method in nutrient agar plate and incubated for 24 hours at 37°C using standard laboratory protocol. Similarly, all the specimens were cultured in Mac Conkey Agar media supplemented with 30 μg/ml of Chloramphenicol and 20 μg/ml of Gentamycin for the enumeration of multiple drug resistant (MDR) bacteria, which were further subjected to in-vitro antibiotic susceptibility test by modified Kirby Bauer disc diffusion technique for resistance patterns. Results: Total viable counts of hospital effluents significantly exceeded the standard heterotrophic plate count (p=0.000). Similarly, the numbers of multiple drug resistant bacteria were alarmingly high in three (more than 30% in 2 and 50% in 1) hospitals of this study. Drug resistant hospital effluent isolates showed simultaneous resistance for most of the antibiotics including Penicillin, Cephalosporin, Cotrimoxazole, Gentamycin and Quinolones. Conclusion: Healthcare liquid wastes were laden with MDR bacteria and seemed to pose a huge public health threat in the transfer of such resistance to the bacterial pathogens causing community acquired infections, thereby limiting our antibiotic pool. Key words: Healthcare liquid waste management; viable count; multiple drug resistance; hospitals; Nepal DOI: 10.3126/kumj.v8i1.3220 Kathmandu University Medical Journal (2010), Vol. 8, No. 1, Issue 29, 40-44

scholarly journals Isoalation of Multidrug Resistant Bacterial Pathogens from Human Hair Obtained from Barbing Salons Located within Benin City, Nigeria

2019 ◽  
pp. 1-7
Author(s):  
Lucky Evbuomwan ◽  
Patrick Emeka Chukwuka ◽  
Fortune Itojie Ebiala
Keyword(s):  
Escherichia Coli ◽  
Human Hair ◽  
Bacterial Pathogens ◽  
Multiple Drug ◽  
Microbiological Analysis ◽  
Drug Resistant ◽  
Proteus Vulgaris ◽  
Benin City ◽  
Multiple Drug Resistant ◽  
Corynebacterium Sp

This study was carried out to investigate the antibiotic susceptibility pattern of bacterial pathogens isolated from human hair in barbing salon. Hair samples were collected from ten different barbing saloons in Benin City and immediately transported to the laboratory for microbiological analysis using pour plate isolation method. Isolated bacteria were identified based on their cultural, morphological and biochemical characteristic. Antibiotics sensitivity was carried out using commercially available antibiotic disks. Total bacteria counts ranged from 2.80x104±0.8cfu/g to 6.13x104±0.21 cfu/g. Bacterial isolated included Escherichia coli, Proteus vulgaris, Streptococcus viridians and Corynebacterium sp. The least occurring bacteria were Escherichia coli and Proteus vulgaris with percentage distribution of 40% each while the most widely distributed was Corynebacterium sp. (80%). All the bacterial isolates were observed to be multiple drug resistant. The most effective drugs were sparfloxacin, perfloxacin, gentamicin, erythromycin and ciprofloxacin. This study has shown that high densities of multiple drug resistant pathogenic bacteria are usually associated with human hair.

scholarly journals trans-Translation inhibitors bind to a novel site on the ribosome and clear Neisseria gonorrhoeae in vivo

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zachary D. Aron ◽  
Atousa Mehrani ◽  
Eric D. Hoffer ◽  
Kristie L. Connolly ◽  
Pooja Srinivas ◽  
...  
Keyword(s):  
Oral Dose ◽  
Neisseria Gonorrhoeae ◽  
Multiple Drug ◽  
Specific Inhibition ◽  
Drug Resistant ◽  
Peptidyl Transfer ◽  
Bacterial Ribosome ◽  
Multiple Drug Resistant ◽  
Unique Site

AbstractBacterial ribosome rescue pathways that remove ribosomes stalled on mRNAs during translation have been proposed as novel antibiotic targets because they are essential in bacteria and are not conserved in humans. We previously reported the discovery of a family of acylaminooxadiazoles that selectively inhibit trans-translation, the main ribosome rescue pathway in bacteria. Here, we report optimization of the pharmacokinetic and antibiotic properties of the acylaminooxadiazoles, producing MBX-4132, which clears multiple-drug resistant Neisseria gonorrhoeae infection in mice after a single oral dose. Single particle cryogenic-EM studies of non-stop ribosomes show that acylaminooxadiazoles bind to a unique site near the peptidyl-transfer center and significantly alter the conformation of ribosomal protein bL27, suggesting a novel mechanism for specific inhibition of trans-translation by these molecules. These results show that trans-translation is a viable therapeutic target and reveal a new conformation within the bacterial ribosome that may be critical for ribosome rescue pathways.

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