Comparative Study of Micro-ID, Minitek and Conventional Methods with Enterobacteriaceae Freshly Isolated from Foods

1979 ◽  
Vol 42 (9) ◽  
pp. 735-738 ◽  
Author(s):  
N. A. COX ◽  
A. J. MERCURI ◽  
M. O. CARSON ◽  
D. A. TANNER
Keyword(s):  
Ground Beef ◽  
Enterobacter Cloacae ◽  
Complete Agreement ◽  
Enterobacter Agglomerans ◽  
Biochemical Tests ◽  
Pork Sausage ◽  
Serratia Liquefaciens ◽  
Conventional Tests ◽  
Conventional Systems ◽  
Raw Shrimp

A total of 400 Enterobacteriaceae isolates freshly taken from broiler carcasses, ground beef, pork sausage, raw shrimp, pre-wrapped sandwiches, raw carrots, lettuce and fresh strawberries was inoculated into the 15 biochemical tests of the Micro-ID (4-h) system and into the 15 corresponding tests in the Minitek (24-h) and conventional systems. For each food there were 750 biochemical test comparisons (50 isolates × 15 tests). The overall agreement between Micro-ID and conventional tests was 96.8%, whereas the agreement between Minitek and conventional tests was 93.6%. Three laboratory technicians who independently recorded results of 6000 biochemical tests from each of the three systems were in complete agreement for 99.3%, 98.9% and 99.7% of the Micro-ID. Minitek and conventional tests, respectively. Thus results obtained with the miniaturized systems were as easy to read and interpret as conventional tests in tubes. The most frequently encountered Enterobacteriaceae from these foods were Escherichia coli (broiler carcasses, pork sausage). Enterobacter agglomerans (carrots, lettuce, shrimp, strawberries), Enterobacter cloacae (pre-wrapped sandwiches), and Serratia liquefaciens (ground beef).

Comparisons of Selected Methods with the Fung-Yu Tube Procedure for Determining Listeria monocytogenes and Other Listeria spp. in Meats

1992 ◽  
Vol 55 (5) ◽  
pp. 349-355 ◽  
Author(s):  
LINDA S. L. YU ◽  
DANIEL Y. C. FUNG
Keyword(s):  
Listeria Monocytogenes ◽  
Dna Hybridization ◽  
Ground Beef ◽  
Culture Broth ◽  
Detection Time ◽  
Biochemical Tests ◽  
Pork Sausage ◽  
Poultry Products ◽  
Enrichment Procedure

The ability of the motility enrichment Fung-Yu tube procedure with Oxyrase™ enzyme to detect the presence of Listeria monocytogenes inoculated into ground beef samples was compared to the USDA-FSIS method. Three strains of L. monocytogenes (LM 101M, LM 103M, and Scott A) were inoculated separately into sterilized ground beef or culture broth. The inoculum levels used were as low as 1 to 1000 Listeria cells per g of meat or per ml of broth. The Fung-Yu tube procedure produced results as sensitive as the USDA procedures and provided a shorter detection time of 26–48 h. A total of 215 retail-level meat and poultry products were analyzed comparatively by the Fung-Yu tube and the GENETRAK® DNA hybridization methods for Listeria detection. Six Listeria spp. (L. denitrificans, L. grayi, L. innocua, L. ivanovii, L. monocytogenes, and L. murrayi) were identified among the isolates. All 48 presumptively positive samples determined by the Fung-Yu tube method were further confirmed to harbor Listeria by biochemical tests. Eleven samples were missed by the GENETRAK® procedure, probably because of the enrichment procedure. L. monocytogenes was isolated from ground beef, pork sausage, smokie links, and cheese hot dogs. Among cooked samples examined, only cheese hot dogs and macaroni and cheese loaf showed substantial incidence of Listeria contamination.

scholarly journals POLA BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL PADA KAMAR BERSALIN RSAD ROBERT WOLTER MONGISIDI MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lisa E. Ritto ◽  
Standy Soeliongan ◽  
Fredine E. S. Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infection ◽  
Staphylococcus Epidermidis ◽  
Enterobacter Cloacae ◽  
Developed Countries ◽  
Aerobic Bacteria ◽  
Enterobacter Agglomerans ◽  
Isolation And Identification ◽  
Serratia Liquefaciens ◽  
Identification Of Bacteria

Abstract: The situation in countries with limited resources, are radically different in almost every aspect, making pregnant women in developing countries prone to a higher risk of getting nosocomial infections after childbirth than their counterparts in developed countries. One of the most common cause of nosocomial infection is bacterial. The aim of this study was to determine the pattern of aerobic bacteria that could potentially cause nosocomial infection in delivery room RSAD Robert Wolter Monginsidi Manado and followed by isolation and identification of bacteria. This research uses descriptive method with prospective approach. Samples were taken by acquiring swabs from the walls, floor, room furniture, medical equipment, and air as much as 25 samples. As a result, there are Lactobacillus sp. 7 samples (28%), Enterobacter agglomerans 7 samples (28%), Bacillus subtilis 6 samples (24%), Staphylococcus epidermidis 3 samples (12%), Serratia liquefaciens 1 samples (4%) and Enterobacter cloacae 1 samples (4%). This study found the most bacteria are Lactobacillus sp. and Enterobacter agglomerans.Keywords: aerobic bacteria, nosocomial infection, delivery room.Abstrak: Situasi di negara-negara dengan sumber-sumber yang terbatas, secara radikal berbeda hampir dalam setiap aspek membuat perempuan hamil di negara-negara sedang berkembang dihadapkan kepada resiko lebih tinggi untuk mendapatkan infeksi nosokomial sesudah persalinan daripada mitranya di negara-negara maju. Salah satu penyebab tersering infeksi nosokomial adalah bakteri. Tujuan dari penelitian ini adalah untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada kamar bersalin RSAD Robert Wolter Mongisidi Manado yang kemudian dilakukan isolasi dan identifikasi bakteri. Penelitian ini menggunakan metode penelitian deskriptif dengan pendekatan prospektif. Sampel diambil secara usapan dari dinding, lantai, perabotan ruangan, peralatan medis, dan udara sebanyak 25 sampel. Hasil penelitian ditemukan Lactobacillus sp. 7 sampel (28%), Enterobacter agglomerans 7 sampel (28%), Bacillus subtilis 6 sampel (24%), Staphylococcus epidermidis 3 sampel (12%), Serratia liquefaciens 1 sampel (4%) dan Enterobacter cloacae 1 sampel (4%). Kesimpulan dari penelitian ini bakteri terbanyak yang ditemukan adalah Lactobacillus sp. dan Enterobacter agglomerans.Kata kunci: bakteri aerob, infeksi nosokomial, kamar bersalin

scholarly journals Isolasi dan identifikasi bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di Irina D RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Windy L. Rengkuan ◽  
Olivia A. Waworuntu ◽  
Standy Soeliongan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Serratia Marcescens ◽  
Nosocomial Infections ◽  
Enterobacter Aerogenes ◽  
Enterobacter Agglomerans ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Serratia Liquefaciens ◽  
Identification Of Bacteria

Abstract: Infection is still one of the major causes of mortality and morbidity in the hospital. In Indonesia, infection is a major cause of maternal death and newborn, and also led to an extension of hospitalization for patients. Nosocomial infections are infections suffered by patients that were admitted to the hospital after ± 72 hours of admission originated from microbiological and environmental factors. Germs that cause most frequent nosocomial infections are Proteus sp, Escherichia coli, Staphylococcus aureus and pseudomonas. This study was aimed to isolate and identify the most common aerobic bacteria that could potentially be the causes of nosocomial infections at IRINA D Prof. Dr. R. D. Kandou Hospital Manado. There were 27 samples consisting of 8 air samples and 19 samples of swab the surface of walls, floors and furniture. Identification of bacteria was performed by using cultures, Gram staining, and biochemical tests. Of the 27 samples 7 types of bacteria are found. Serratia liquefaciens is the largest with 12 samples (44.4%) Basillus subtilis with 7 samples (25.9%), Enterobacter aerogenes in 3 samples (11.1%), Serratia marcescens in 2 samples (7.4%), while Enterobacter agglomerans, staphylococcus sp, coccus Gram negative each in one sample (each of 3.7%). Gram-negative bacteria were the most common bacteria found at IRINA D RSUP Prof. Dr. R. D. Kandou Manado. Serratia liquefaciens was the main bacteria considered to be potentially the cause nosocomial infection.Keywords: identification of bacteria, nosocomial infections, inpatient installation Abstrak: Infeksi masih merupakan salah satu penyebab utama kematian dan kesakitan di rumah sakit. Di Indonesia sendiri infeksi merupakan salah satu penyebab utama kematian ibu dan bayi baru lahir dan juga menyebabkan perpanjangan masa rawat inap bagi penderita. Infeksi nosokomial merupakan infeksi yang tidak diderita pasien saat masuk ke rumah sakit melainkan setelah ±72 jam berada di tempat tersebut yang berasal dari faktor mikrobiologis dan faktor lingkungan. Kuman penyebab infeksi nosokomial yang paling sering ialah Proteus sp, Escherichia coli, Staphylococcus aureus dan Pseudomonas. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri aerob terbanyak yang berpotensi menjadi penyebab infeksi nosokomial di irina D RSUP Prof. Dr. R. D. Kandou Manado. Sampel diambil sebanyak 27 sampel yang terdiri dari 8 sampel udara dan 19 sampel dari swab permukaan dinding, lantai dan perabotan. Untuk identifikasi bakteri dilakukan kultur pada media agar selanjutnya dilakukan pewarnaan Gram dan uji biokimia. Dari 27 sampel ditemukan 7 jenis bakteri. Serratia liquefaciens merupakan bakteri terbanyak dengan 12 sampel (44,4%) Basillus subtilis dengan 7 sampel (25,9%), Enterobacter aerogenes dengan 3 sampel (11,1%), Serratia marcescens dengan 2 sampel (7,4%), sedangkan Enterobacter agglomerans, Staphylococcus sp, coccus Gram (-) masing-masing didapatkan 1 sampel dengan presentase masing-masing 3,7%. Bakteri Gram negatif merupakan bakteri yang paling banyak ditemukan pada instalasi rawat inap D RSUP Prof. Dr. R. D. Kandou Manado. Serratia liquefaciens merupakan bakteri yang berpotensi menjadi penyebab infeksi nosokomial terbanyak yang ditemukan.Kata kunci: identifikasi bakteri, infeksi nosokomial, instalasi rawat inap

Coliforms and Enterobacteriaceae Isolates from Selected Foods

1979 ◽  
Vol 42 (9) ◽  
pp. 712-714 ◽  
Author(s):  
A. J. MERCURI ◽  
N. A. COX
Keyword(s):  
Ground Beef ◽  
Gas Production ◽  
Fecal Coliforms ◽  
Poultry Meat ◽  
Type I ◽  
Enterobacter Agglomerans ◽  
Pork Sausage ◽  
E Coli ◽  
System A ◽  
Principal Species

We obtained coliform counts and Enterobacteriaceae counts using violet red bile agar (VRB) and VRB + 1% glucose (VRBG), respectively, of samples of five food products. From each set of VRB and VRBG plates, 28 to 40 “typical” colonies were randomly selected and identified by use of the R-B Enteric Differential System. A pure culture of each isolate was also subjected to the sequential tests for gas production in LST and BGLB broths (confirmed coliforms) and in EC broth at 45.5 C (fecal coliforms). IMViC reaction patterns of EC-positive cultures were also determined. Approximately 80% of the VRB isolates from broiler skin and from mechanically deboned poultry meat (MDPM) met all the criteria for fecal coliforms, whereas only 62.5% and 36.5%, respectively, of the VRBG isolates from these two products met these criteria. Fewer than 10% of the VRB and VRBG isolates from chicken pot pie, ground beef, or pork sausage produced gas in LST broth. The percentages of fecal coliforms and Escherichia coli (Type I or II) among the 179 VRB isolates were 34.1 and 33.5, respectively. Corresponding percentages for the 193 VRBG isolates were 20.7 and 19.7. E. coli was the predominant species isolated on both media from broiler skin and MDPM. Enterobacter agglomerans was the principal species isolated from chicken pot pie and pork sausage; Serratia liquefaciens predominated in ground beef.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG DAPAT MENYEBABKAN INFEKSI NOSOKOMIAL DI RUANGAN INSTALASI GIZI BLU RSUP PROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Priska N. Riga ◽  
Velma Buntuan ◽  
Fredine Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infections ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Enterobacter Agglomerans ◽  
Vibrio Cholera ◽  
Providencia Rettgeri ◽  
Serratia Liquefaciens ◽  
Providencia Stuartii ◽  
Serratia Rubidaea

Abstract: Kitchen of hospital to be a part or unit of a hospital that is not less important as other services in the hospital , because it provides direct services to patients through the food served by nutritional care . Nosocomial infections are infections that occur during hospitalization where the infection does not exist at the time of admission to the hospital . Infections that occur more than 48 hours after hospital admission . Most infections occur in hospitals is caused by external factors , ie diseases which spread from food and cutlery . Implementation of the food was less qualified health care, in addition to extending the treatment process can also cause cross-infections or nosocomial infections which can be through the cutlery and food . Purpose: To determine the presence of aerobic bacteria that can cause nosocomial infections in the kitchen of BLU Prof. Dr R. D. Kandou Manado .Method: This study was a prospective descriptive study that analyzed 24 samples on cutlery and 6 samples of food in kitchen of BLU Nutrition Dr Prof. Dr R. D. Kandou Manado. Result: From the results of this study were found 11 species of bacteria such as Bacillus subtilis (33,3%), a gram-negative cocci (10%) , Lactobacillus (10%), Enterobacter agglomerans (6,7%), Serratia rubidaea (6,7%), Providencia stuartii (3,3%), Serratia liquefaciens (3,3%), Providencia rettgeri (3,3%), Vibrio cholera (3,3%), Enterobacter cloacae (3,3%), Enterobacter aerogenes (3,3%).Conclusion: The conclusion of this study showed that the most commonly found bacteria was Bacillus subtilis (33,3%).Keywords: nosocomial infection, kitchen of hospital, bacteria.Abstrak: Instalasi gizi menjadi bagian atau unit kerja di rumah sakit yang tidak kalah pentingnya dengan pelayanan lain di rumah sakit, karena memberikan pelayanan langsung kepada pasien melalui makanan yang disajikan oleh asuhan gizi. Infeksi nosokomial adalah infeksi yang terjadi selama perawatan di rumah sakit dimana infeksi tersebut tidak ada pada saat pasien masuk ke rumah sakit. Infeksi yang timbul lebih dari 48 jam setelah masuk rumah sakit. Kebanyakan infeksi yang terjadi di rumah sakit lebih disebabkan oleh faktor eksternal, yaitu penyakit yang penyebarannya ke makanan dan alat makan. Penyelenggaraan makanan yang kurang memenuhi syarat kesehatan, selain memperpanjang proses perawatan juga dapat menyebabkan timbulnya infeksi silang atau infeksi nosokomial yang diantaranya dapat melalui alat makan dan makanan. Tujuan: Untuk mengetahui adanya bakteri aerob yang dapat menyebabkan infeksi nosokomial di ruangan Instalasi Gizi BLU RSUP Prof. Dr. R. D. Kandou Manado. Metode penelitian: Penelitian ini merupakan penelitian deskriptif prospektif yang dianalisis secara desktriptif pada 24 sampel alat makan dan 6 sampel makanan di ruangan Instalasi Gizi BLU RSUP Prof. Dr. R. D. Kandou Manado. Hasil: Dari hasil penelitian ditemukan 11 spesies bakteri yaitu Bacillus subtilis (33,3%), Kokus gram negatif (10%), Lactobacillus (10%), Enterobacter agglomerans (6,7%), Serratia rubidaea (6,7%), Providencia stuartii (3,3%), Serratia liquefaciens (3,3%), Providencia rettgeri (3,3%), Vibrio cholera (3,3%), Enterobacter cloacae (3,3%), Enterobacter aerogenes (3,3%). Kesimpulan: Kesimpulan dari penelitian ini menunjukkan bahwa bakteri yang paling banyak ditemukan dalam penelitian ini adalah Bacillus subtilis (33,3%).Kata Kunci: infeksi nosokomial, instalasi gizi, bakteri.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI RUANG RAWAT INAP MATA IRINA F RSUP PROF. DR. R.D. KANDOU MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ameliya S. Japanto ◽  
Standy Soeliongan ◽  
Fredine E. S. Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infections ◽  
Agar Medium ◽  
Enterobacter Cloacae ◽  
Hospital Environment ◽  
Aerobic Bacteria ◽  
Enterobacter Agglomerans ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Identification Of Bacteria

Abstract: Nosocomial infections are infections acquired or occurring while patients were hospitalized. Nosocomial infections can be caused by various agents of disease, like bacteria. The bacteria are found in the hospital environment, including the inpatient unit. Objective: to know the pattern of aerobic bacteria that could potentially cause nosocomial infections in inpatient eyes unit IRINA F Prof. Dr. R. D. Kandou Manado. Methods: 14 swabs were taken at the surface of patient’s beds, treatment rooms and 8 samples of air space. Identification of bacteria carried on an agar medium isolation, Gram staining and biochemical tests. Results: The obtained bacteria identified six types of bacteria, such as Bacillus subtilis, Lactobacillus sp., Coccus negative Gram, Enterobacter agglomerans, Enterobacter cloacae, and Staphylocccus epidermidis. Conclusion: Bacillus subtilis is the bacteria that most commonly found in inpatient eyes unit IRINA FKeywords: nosocomial infections, patterns of aerobic bacteria, inpatient eye unitAbstrak: Infeksi nosokomial adalah infeksi yang didapat atau terjadi saat pasien dirawat di rumah sakit. Infeksi nosokomial dapat disebabkan oleh berbagai agen penyakit, salah satunya ialah bakteri. Bakteri penyebab infeksi sering ditemukan di lingkungan rumah sakit, termasuk di ruang rawat inap. Tujuan : mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruang rawat inap mata IRINA F RSUP Prof. Dr. R. D. Kandou Manado. Metode: sampel diambil pada 14 usapan perabotan ruangan, ruangan perawatan dan 8 sampel udara ruang. Identifikasi bakteri dilakukan isolasi pada media agar, pewarnaan Gram dan uji biokimia. Hasil: bakteri yang teridentifikasi didapatkan enam jenis bakteri, yaitu Bacillus subtilis, Lactobacillus sp., Coccus Gram negatif, Enterobacter agglomerans, Enterobacter cloacae, dan Staphylocccus epidermidis. Kesimpulan: Bakteri Bacillus subtilis merupakan bakteri yang paling banyak ditemukan di ruang rawat inap mata IRINA FKata kunci: infeksi nosokomial, pola bakteri aerob, ruang rawat inap mata

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI IRINA E RSUP PROF. DR. R. D. KANDOU MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Beatrix A. Lumentut ◽  
Olivia A. Waworuntu ◽  
Heriyannis Homenta
Keyword(s):  
Health Care ◽  
Bacillus Subtilis ◽  
Nosocomial Infections ◽  
Medical Equipment ◽  
Enterobacter Cloacae ◽  
Enterobacter Agglomerans ◽  
Healthcare Associated Infection ◽  
Cross Sectional ◽  
Serratia Liquefaciens ◽  
Healthcare Associated

Abstract: Nosocomial infections or now called Healthcare Associated Infection (HAIs) is an infection that occurs in patients during treatment in a hospital or other health care facilities that do not show symptoms of infection while in the hospital. Nosocomial infection is an important issue in health care that continues to increase especially in children who are under treatment in hospital. This study aims to determine the source and identify aerobic bacteria that could potentially cause nosocomial infections in walls, floors, medical equipment, and air in Irina E Prof. Dr. R. D. Kandou Manado General Hospital. Design methods for this research using cross sectional study with a descriptive prospective approach. Samples were bacteria in walls, floors, medical equipment, and air of Irian E Prof. Dr. R. D. Kandou Manado General Hospital. The results showed that from the 24 samples taken, there were 6 bacteria found which is Enterobacter cloacae (41,7%), Bacillus subtilis (20,8%), Serratia liquefaciens (16,7%), Enterobacter agglomerans (12,5%), Lactobacillus (4,17%), dan Staphylococcus sp. (4,17%),Keywords: Irina E, nosocomial infections, aerobic bacteria.Abstrak: Infeksi nosokomial atau yang sekarang disebut Healthcare Associated Infection (HAIs) adalah infeksi yang terjadi pada pasien selama proses perawatan di rumah sakit atau fasilitas kesehatan lainnya yang tidak menunjukkan gejala infeksi saat masuk rumah sakit. Infeksi nosokomial merupakan masalah penting dalam pelayanan kesehatan yang terus meningkat terlebih pada anak-anak yang sedang dalam perawatan di rumah sakit. Penelitian ini bertujuan untuk mengetahui sumber dan mengidentifikasi bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di dinding, lantai, peralatan medis, dan udara di Irina E RSUP Prof. Dr. R. D. Kandou Manado. Desain penelitian ini menggunakan studi cross sectional dengan pendekatan deskriptif prospektif. Sampel penelitian ialah bakteri pada dinding, lantai, peralatan medis, dan udara di Irina E RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian memperlihatkan dari 24 sampel yang diambil, terdapat 6 bakteri yaitu Enterobacter cloacae (41,7%), Bacillus subtilis (20,8%), Serratia liquefaciens (16,7%), Enterobacter agglomerans (12,5%), Lactobacillus (4,17%), dan Staphylococcus sp. (4,17%),Kata kunci: Irina E, infeksi nosokomial, bakteri aerob

scholarly journals Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil

2021 ◽  
Vol 14 ◽  
pp. 117863612110242
Author(s):  
Sonal Suman ◽  
Tanuja
Keyword(s):  
Contaminated Soil ◽  
Growth Parameters ◽  
Chemical Properties ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Bacterial Degradation ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
16S Rna ◽  
Degrading Bacteria

DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that Enterobacter cloacae had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that Enterobacter cloacae was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.

scholarly journals Incidence and contamination level of Clostridium perfringens in meat and meat products sold in Sakarya province of Turkey

2021 ◽  
Vol 7 (3) ◽  
pp. 172-178
Author(s):  
Serap Coşansu ◽  
Şeyma Şeniz Ersöz
Keyword(s):  
Clostridium Perfringens ◽  
Meat Product ◽  
Meat Products ◽  
Ground Beef ◽  
Chicken Meat ◽  
Contamination Level ◽  
Biochemical Tests ◽  
Contamination Levels ◽  
Emulsified Meat ◽  
Cooked Meat

Totally 101 meat and meat product samples obtained from local markets and restaurants were analyzed for incidence and contamination level of Clostridium perfringens. The typical colonies grown anaerobically on Tryptose Sulfite Cycloserine Agar supplemented with 4-Methyliumbelliferyl (MUP) were confirmed by biochemical tests. Forty-eight of the samples (47.5%) were contaminated with C. perfringens. The highest incidence of the pathogen was determined in uncooked meatball samples (72.2%) followed by ground beef samples (61.3%). The incidence of C. perfringens in chicken meat, cooked meat döner, cooked chicken döner and emulsified meat product samples were 33.3, 33.3, 28.6 and 16.7%, respectively. Thirteen out of 101 samples (12.9%) yielded typical colonies on TSC-MUP Agar, but could not be confirmed as C. perfringens. Average contamination levels in sample groups ranged from 8.3 to 1.5×102 cfu/g, with the highest ground beef and the lowest chicken meat.

Enterobacteriaceae in ground meats

1978 ◽  
Vol 24 (12) ◽  
pp. 1574-1582 ◽  
Author(s):  
Lai-King Ng ◽  
Michael E. Stiles
Keyword(s):  
Ground Beef ◽  
False Positives ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Type I ◽  
Enterobacter Agglomerans ◽  
Frozen Ground ◽  
Probable Number ◽  
E Coli ◽  
Solid Media

Presumptive Escherichia coli counts for 312 samples of non-frozen ground beef were determined and compared with proposed Canadian standards. Results for frozen pork sausages, packaged at manufacturer level, indicated little difference in distribution of presumptive E. coli loads compared with retail ground beef. Use of solid media and direct inoculation of EC broth at 45 °C did not give alternative, rapid methods of estimating E. coli loads in ground beef. Counts on violet red bile agar (VRBA) within 18–24 h incubation at 35 °C gave reliable estimates of coliform bacteria (bile-precipitating colonies) and Enterobacteriaceae (total count), with only 1.3 and 10.7% false positives, respectively. Bile-precipitating isolates from VRBA were primarily E. coli, also Serratia liquefaciens, aerogenic Enterobacter agglomerans, Enterobacter cloacae, Citrobacter freundii, and Klebsiella pneumoniae. Non-bile-precipitating colonies were primarily aerogenic E. agglomerans and S. liquefaciens; however, in the most probable number technique E. agglomerans was screened out. In addition to E. coli, E. agglomerans and S. liquefaciens were the principal types of Enterobacteriaceae in these samples. Enterobacter agglomerans gave a variety of IMViC reactions, including the type I (++−−) reaction, whereas S. liquefaciens were predominantly IMViC type −−++. Incubating EC broth at 45.5 °C, as opposed to 44.5 °C, reduced the number of false positives.

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