Effect of Processing and Storing Oyster Meats on Concentrations of Indicator Bacteria, Vibrios and Aeromonas hydrophila

1984 ◽  
Vol 47 (8) ◽  
pp. 598-601 ◽  
Author(s):  
M. A. HOOD ◽  
R. M. BAKER ◽  
F. L. SINGLETON

Oyster meats were examined from three commercial processing plants at different stages of processing and storage for four standard indicator bacterial groups, five species of vibrios reported to be associated with shellfish associated gastroenteritis, and Aeromonas hydrophila. Processing reduced the overall microbial load, but the individual bacterial groups examined remained statistically the same throughout processing. Upon storage, the concentration of Vibrio parahaemolyticus significantly declined with a concomitant increase in levels of A. hydrophila, and the levels of all other Vibrio spp. remained statistically the same. The findings suggest that, while processing results in a cleaner appearing product, processing does not eliminate potentially pathogenic vibrios. However, processing and subsequent storage of oyster meats do not appear to increase the levels of vibrios.

2014 ◽  
Vol 138 (11) ◽  
pp. 1481-1487 ◽  
Author(s):  
Christopher A. Tormey ◽  
Gary Stack

Context The extent to which changes in secretory function contribute to the storage lesion of platelets (PLTs) prepared for transfusion is not well described. Objective To develop a cytokine-release assay for the assessment of PLT secretory capacity during the preparation and storage of PLTs. Design Small volumes of PLT-rich plasma and PLT concentrate (PC) were prepared from whole blood (WB; N = 4 donors). Aliquots of WB, PLT-rich plasma, and PC were treated with 20 μM adenosine diphosphate or saline (control). Samples of WB-derived PCs obtained from a regional blood center were similarly stimulated at various storage times (N = 10 units). Plasma levels of RANTES (chemokine ligand 5; regulated on activation, normal T cell expressed and secreted) and PLT aggregation were measured following agonist addition. Results Adenosine diphosphate stimulated RANTES release from PLTs in fresh WB on average by 4.1-fold (P < .001), in PLT-rich plasma by 4.7-fold (P = .002), and in PC by 1.3-fold (P < .001). For blood center PCs, adenosine diphosphate failed to stimulate RANTES release at day 2 of storage or later (P ≥ .31). Baseline RANTES levels in the plasma/supernatant increased 660% during PC preparation (P = .02) and an additional 30% during subsequent storage (P < .001). Mean PLT aggregation decreased during processing from WB (95.6%) to PC (60.5%; P = .04). For blood center PCs, mean PLT aggregation decreased substantially from days 2 (41.0%) to 7 (2.3%; P < .001). Conclusions A cytokine-release assay revealed a diminution in PLT secretory capacity during PC processing and storage, with complete elimination by day 2 of storage. Loss of PLT aggregability occurred more slowly. The cytokine-release assay may be a useful endpoint for optimizing PLT preparation and storage.


2012 ◽  
Vol 195-196 ◽  
pp. 1265-1270
Author(s):  
Feng Ying Xu ◽  
Zhen Chen ◽  
Peng Cheng Wang ◽  
Xiang Jun Zou ◽  
Ce Xu ◽  
...  

The research on the damage banana quality with loaded mechanical energy has significance to its storage, but there are few researches at present. This paper investigated 3 indexes of the energy and substance transformation in the damage banana by loading, including the change of the respiration rate CO2, the maximum impact stress intensity of fruit re-enduring and the maximum mechanical energy etc. during the storage. The results showed that there existed a significant association between the energy conversion and quality characteristics for the damaged banana, and the energy transformation affected the individuals physiological and biochemical process. There presented a significant trend on the intensity of the loading mechanical energy against the quality characteristics on above 3 indexes during the storage, but the difference between the individual had little effects on it. Decreasing the energy and substance transformation in damage area are the important means of extending the physiology life of fruits, which is an urgent problem to be solved in their field of processing and storage at present.


Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 673
Author(s):  
Xiong Luo ◽  
Lijia Xu ◽  
Peng Huang ◽  
Yuchao Wang ◽  
Jiang Liu ◽  
...  

Nondestructive detection of tea’s internal quality is of great significance for the processing and storage of tea. In this study, hyperspectral imaging technology is adopted to quantitatively detect the content of tea polyphenols in Tibetan teas by analyzing the features of the tea spectrum in the wavelength ranging from 420 to 1010 nm. The samples are divided with joint x-y distances (SPXY) and Kennard-Stone (KS) algorithms, while six algorithms are used to preprocess the spectral data. Six other algorithms, Random Forest (RF), Gradient Boosting (GB), Adaptive boost (AdaBoost), Categorical Boosting (CatBoost), LightGBM, and XGBoost, are used to carry out feature extractions. Then based on a stacking combination strategy, a new two-layer combination prediction model is constructed, which is used to compare with the four individual regressor prediction models: RF Regressor (RFR), CatBoost Regressor (CatBoostR), LightGBM Regressor (LightGBMR) and XGBoost Regressor (XGBoostR). The experimental results show that the newly-built Stacking model predicts more accurately than the individual regressor prediction models. The coefficients of determination Rc2 andRp2 for the prediction of Tibetan tea polyphenols are 0.9709 and 0.9625, and the root mean square error RMSEC and RMSEP are 0.2766 and 0.3852 for the new model, respectively, which shows that the content of Tibetan tea polyphenols can be determined with precision.


Author(s):  
Aulia Andhikawati ◽  
Dian Yuni Pratiwi

Fish that have been caught are susceptible to decay and damage which are influenced by the level of acidity, weather, processing and storage methods, and temperature during transportation. This causes losses to the fishery business. One way that can be used to reduce the damage and spoilage of fish is through smoking. The purpose of this review article is to describe various methods of fish smoking, the chemical composition of smoked fish, and the number of microbes present in smoked fish. Smoked fish still contains nutrients such as protein, lipids, fiber, amino acids, minerals, and vitamins. The growth of microbes, especially those that are pathogenic, is inhibited and even some microbes cannot grow so that smoked fish is still good for human consumption. The microbes that cannot grow include Vibrio spp, yeast, mold, and Salmonella sp. Hot smoking method uses a temperature of 30-90oC, while cold smoking uses a temperature of 30-40oC. The chemical composition contained in smoked fish such as water content, protein, ash content, crude fiber, amino acids, biogenic amines, minerals and phenols. While the microbiological content of smoked fish with hot and cold smoking methods has a TPC value according to smoked fish standards and is free from pathogenic microbes, fungi, yeast and molds.


1989 ◽  
Vol 52 (1) ◽  
pp. 13-20 ◽  
Author(s):  
MOHAMED B. GAILANI ◽  
DANIEL Y. C. FUNG

The Sudanese dry meat SHARMOOT is a major food product in East Africa. No information is available on microbial profiles or improved methods of processing the product. We have developed a pre-cooking and grinding procedure that produces the meat efficiently. The product is chemically and microbiologically stable for at least 4 months without refrigeration. While staphylococci and Enterobacteriaceae were the most common major bacterial groups isolated from dried meat samples at the beginning, micrococci and bacilli predominated during the last stages of storage. Microbiological data (total, spore, yeast and mold, and Staphylococcus aureus counts and Clostridium perfringens detection) indicated that the product made by us is microbiologically more acceptable than a comparable product made traditionally in Sudan. The potential exists for large-scale production of SHARMOOT.


2018 ◽  
Vol 2 (1) ◽  
pp. 43
Author(s):  
Suwignyo Suwignyo ◽  
Abdul Rachim ◽  
Arizal Sapitri

Ice is a water that cooled below 0 °C and used for complement in drink. Ice can be found almost everywhere, including in the Wahid Hasyim Sempaja Roadside. From the preliminary test, obtained 5 samples ice cube were contaminated by Escherichia coli. The purpose of this study was to determine relationship between hygiene and sanitation with presence of Eschericia coli in ice cube of home industry at Wahid Hasyim Roadside Samarinda. This research used quantitative with survey methode. The population in this study was all of the seller in 2nd Wahid Hasyim Roadside. Sample was taken by Krejcie and Morgan so the there were 44 samples and used Cluster Random Sampling. The instruments are questionnaries, observation and laboratory test. Data analysis was carried out univariate and bivariate (using Fisher test p= 0.05). The conclusion of this study there are a relation between chosing raw material (p=0,03) and saving raw material (p=0,03) with presence of Eschericia coli. There was no relation between processing raw material into ice cube with presence of Eschericia coli (p=0,15).Advice that can be given to ice cube should maintain hygiene and sanitation of the selection, processing and storage of ice cube.


2018 ◽  
Vol 2 (95) ◽  
pp. 69-72
Author(s):  
Yu.A. Tarariko ◽  
L.V. Datsko ◽  
M.O. Datsko

The aim of the work is to assess the existing and prospective models for the development of agricultural production in Central Polesie on the basis of economic feasibility and ecological balance. The evaluation of promising agricultural production systems was carried out with the help of simulation modeling of various infrastructure options at the levels of crop and multisectoral specialization of agroecosystems. The agro-resource potential of Central Polesie is better implemented in the rotation with lupine, corn and flax dolguntsem with well-developed infrastructure, including crop, livestock units, grain processing and storage systems, feed, finished products and waste processing in the bioenergetic station. The expected income for the formation of such an infrastructure is almost 8 thousand dollars. / with a payback period of capital investments of 2-3 years.


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