Microbiological Profile and Storage Temperatures of Egyptian Rice Dishes

1985 ◽  
Vol 48 (1) ◽  
pp. 39-43 ◽  
Author(s):  
M. R. EL-SHERBEENY ◽  
M. FAHMI SADDIK ◽  
HEKMAT EL-SAID ALY ◽  
FRANK L. BRYAN

Cooked rice and other dishes containing rice (172 samples) were tested for aerobic colony count (30°C), Salmonella, Shigella, Staphylococcus aureus, and presumptive Bacillus cereus. Salmonella was isolated from a sample of Oriental rice that was prepared in a five-star hotel kitchen. Shigella was isolated from a sample of boiled rice from a four-star hotel kitchen. Nineteen percent of the samples were contaminated by S. aureus, and 73% of these contained more than 103/g. Forty percent of the samples were contaminated by B. cereus, 31% of these contained more than 103/g. Rice was more frequently stored at safe temperatures (>55°C) in four- and five-star hotels than in any other type of establishment or that which was sold by street venders. Aerobic colony counts (30°C) per g were usually quite low when rice was held at temperatures of 55°C or higher. These counts generally became progressively higher as the temperature decreased, often reaching quantities exceeding 106 when temperatures were 44°C or below. This was particularly so when the temperature range was 25–34°C. These counts were lower for fried and Oriental rice than for boiled rice, rice and vegetables, kushari (a mixture of rice, macaroni and lentils), and rice and shirea (thin, wheat macaroni).

1977 ◽  
Vol 78 (3) ◽  
pp. 387-394 ◽  
Author(s):  
J. A. Pinegar ◽  
J. D. Buxton

SUMMARYOne hundred and thirty-three vanilla slices, purchased from shops in the West Yorkshire Metropolitan County, were examined to determine the numbers and types of bacteria present at the time of purchase. The surface colony count at 37 °C was > 103/g in 67/133 (50%) of the samples examined,Bacillus cereusbeing found at that concentration in 21·8%, coliform bacilli includingE. coliin 5·3%,Staphylococcus aureusin 3·0% andStreptococcus faecalisin 0·8%. Thirty-four strains ofB. cereuswere serotyped and 11 (32%) of these were typable with the sera available.Preparation of custard mixes in the laboratory suggests that the milk or milk powder used in the mix may be the major source ofB. cereusin the final product. Many of the present methods of manufacture, distribution and storage allow organisms present in the custard at manufacture the opportunity to multiply and possibly reach numbers which present a risk of food poisoning.


2016 ◽  
Vol 79 (1) ◽  
pp. 148-152 ◽  
Author(s):  
TIAN DING ◽  
YAN-YAN YU ◽  
CHENG-AN HWANG ◽  
QING-LI DONG ◽  
SHI-GUO CHEN ◽  
...  

ABSTRACT The objectives of this study were to develop a probability model of Staphylococcus aureus enterotoxin A (SEA) production as affected by water activity (aw), pH, and temperature in broth and assess its applicability for milk. The probability of SEA production was assessed in tryptic soy broth using 24 combinations of aw (0.86 to 0.99), pH (5.0 to 7.0), and storage temperature (10 to 30°C). The observed probabilities were fitted with a logistic regression to develop a probability model. The model had a concordant value of 97.5% and concordant index of 0.98, indicating that the model satisfactorily describes the probability of SEA production. The model showed that aw, pH, and temperature were significant factors affecting the probability of toxin production. The model predictions were in good agreement with the observed values obtained from milk. The model may help manufacturers in selecting product pH and aw and storage temperatures to prevent SEA production.


1974 ◽  
Vol 73 (3) ◽  
pp. 433-444 ◽  
Author(s):  
R. J. Gilbert ◽  
M. F. Stringer ◽  
T. C. Peace

SummaryA number of outbreaks of food poisoning attributed toBacillus cereushave been reported recently and all have been associated with cooked rice usually from Chinese restaurants and ‘take-away’ shops.Tests were made to assess the heat resistance ofB. cereusspores in aqueous suspension, the growth of the organism in boiled rice stored at temperatures in the range 4–55° C., and the effect of cooking and storage on the growth of the organism in boiled and fried rice. The spores ofB. cereussurvived cooking and were capable of germination and outgrowth. The optimum temperature for growth in boiled rice was between 30° and 37° C. and growth also occurred during storage at 15° and 43° C.To prevent further outbreaks it is suggested that rice should be boiled in smaller quantities on several occasions during the day, thereby reducing the storage time before frying. After boiling the rice should either be kept hot (> 63° C.) or cooled quickly and transferred to a refrigerator within 2 hr. of cooking. Boiled or fried rice must not be stored under warm conditions especially in the range 15–50° C.


1987 ◽  
Vol 50 (2) ◽  
pp. 136-140 ◽  
Author(s):  
D.W. WARBLTRTON ◽  
P.I. PETERKIN ◽  
G.A. JARVIS ◽  
K.F. WEISS ◽  
G. RIEDEL

A study done in 1977–1978, assessed the bacteriological quality of five types of dry desserts including starch-, gelatin- and rennet- based products. One hundred and ninety-seven lots were randomly selected across Canada and analyzed for aerobic colony count, aerobic sporeformers, Bacillus cereus, coliforms, Escherichia coli, Staphylococcus aureus and Salmonella. Micro-biological and practical consideration do not warrant the establishment of standards or guidelines for such products at this time.


1997 ◽  
Vol 60 (12) ◽  
pp. 1541-1547 ◽  
Author(s):  
WOOI FANG NG ◽  
BRUCE E. LANGLOIS ◽  
WILLIAM G. MOODY

Whole dry-cured (country-style) hams from six manufacturers were sliced and the slices randomly allotted into five treatment groups per manufacturer. One treatment group served as a control and slices in the four other treatment groups were inoculated with approximately 105 CFU/g of ham of either Escherichia coli O157:H7, Listeria monocytogenes, a mixture of three Salmonella spp. (Salmonella typhimurium, Salmonella enteritidis and Salmonella choleraesuis), or Staphylococcus aureus. All ham slices were vacuum-packaged with half of the packages in each treatment group stored at 25°C and half stored at 2°C. Two packages from each manufacturer for each treatment and storage temperature were examined after storage for 0,7, 14,21, and 28 days. S. aureus was detected in 2 of 60 control slices, Salmonella in 2 of 120, L. monocytogenes in 4 of 120, and E. coli O157:H7 was not detected in any of the 120 control ham slices analyzed before or after storage. Aerobic (26 and 35°C) populations of the control vacuum-packaged hams slices increased (P < 0.05) with storage time and the increase in populations was greater (P < 0.05) in vacuum-packaged hams slices at 25 than at 2°C. The extent of the decreases in populations of the inoculated pathogens during storage of vacuum-packaged dry-cured ham slices varied with manufacturer (P < 0.05) and storage temperature (P < 0.05). Decreases in Salmonella spp. and E. coli O157:H7 populations were greater (P < 0.05) in slices stored at 25 than at 2°C, while decreases in L. monocytogenes were similar at both storage temperatures. Staphylococcus aureus enterotoxin was not detected in either S. aureus-inoculated or control ham slices after storage for 28 days. Survival of these pathogens in vacuum-packaged dry-cured ham slices suggests that contaminated hams may pose a safety risk to consumers if consumed without adequate cooking.


2018 ◽  
Vol 81 (3) ◽  
pp. 430-436 ◽  
Author(s):  
Vijay K. Juneja ◽  
Tim B. Mohr ◽  
Meryl Silverman ◽  
O. Peter Snyder

ABSTRACT The objective of this study was to assess the ability of Bacillus cereus spores to germinate and grow in order to determine a safe cooling rate for cooked rice, beans, and pasta, rice-chicken (4:1), rice-chicken-vegetables (3:1:1), rice-beef (4:1), and rice-beef-vegetables (3:1:1). Samples were inoculated with a cocktail of four strains of heat-shocked (80°C for 10 min) B. cereus spores (NCTC 11143, 935A/74, Brad 1, and Mac 1) to obtain a final spore concentration of approximately 2 log CFU/g. Thereafter, samples were exponentially cooled through the temperature range of 54.5 to 7.2°C in 6, 9, 12, 15, 18, and 21 h. At the end of the cooling period, samples were removed and plated on mannitol egg yolk polymyxin agar. The plates were incubated at 30°C for 24 h. The net B. cereus growth from spores in beans was <1 log after 9 h of cooling, but the pathogen grew faster in rice and pasta. In combination products, the net growth was as follows: 3.05, 3.89, and 4.91 log CFU/g in rice-chicken; 3.49, 4.28, and 4.96 log CFU/g in rice-beef; 3.50, 4.20, and 5.32 CFU/g in rice–chicken–mixed vegetables; and 3.68, 4.44, and 5.25 CFU/g in rice–beef–mixed vegetables after 15, 18, and 21 h of cooling, respectively. This study suggests safe cooling rates for cooling cooked rice, beans, pasta, rice-chicken, rice-chicken-vegetables, rice-beef, and rice-beef-vegetables to guard against the hazards associated with B. cereus.


1982 ◽  
Vol 45 (6) ◽  
pp. 527-532 ◽  
Author(s):  
C. A. ROTHENBERG ◽  
B. W. BERRY ◽  
J. L. OBLINGER

Effects of various handling, packaging, temperature-abuse and storage conditions were determined on the microbiological characteristics of beef livers and tongues. These organs were evaluated: (a) initially following slaughter, (b) immediately following the frozen storage period of 2–4 weeks at −29°C and (c) following a simulated shipping-temperature abuse of 24 h at 22–28°C followed by 13 days of storage at −1 ± 0.5°C. Initial counts (log/cm2) of coliforms, coagulase-positive Staphylococcus aureus and Clostridium perfringens ranged from 0.19–1.37. Generally, neither freezing nor temperature-abuse had a significant effect on these microorganisms. Vacuum-packaged beef tongues and livers, generally, had lower bacterial counts than did either naked or polyvinyl chloride film-wrapped products. Generally, it was observed that abusive storage temperatures, in conjunction with the naked and film-wrapped packaging systems, appear to present potential microbial spoilage problems when compared with vacuum packaging.


1981 ◽  
Vol 44 (7) ◽  
pp. 500-512 ◽  
Author(s):  
FRANK L. BRYAN ◽  
CHARLES A. BARTLESON ◽  
NORMA CHRISTOPHERSON

Hazard analyses were conducted in six Cantonese-style restaurants to evaluate the amount of Bacillus cereus in rice and the water activity and the temperatures of rice at each stage of processing. Each of 16 samples of raw, polished rice contained B. cereus. The water activity of cooked rice ranged from 0.912 to 0.980, and was related to the stage of the processing and storage practice. Rice reached temperatures that exceeded 93 C (200 F) during cooking. Cooked rice held in steam tables was maintained at temperatures that should preclude growth of B. cereus. Whenever cooked rice was kept at room temperature for a few hours, the temperatures became such that considerable growth of B. cereus could have occurred. Rice in layers less than 9 cm (3.5 in.) thick cooled rather rapidly; layers thicker than 9 cm (3.5 in.) cooled more slowly. During frying and refrying, temperatures exceeded 74 C (165 F). B. cereus was frequently isolated from rice at various stages of preparation and storage, but in numbers fewer than 103 per g. This organism was also isolated from rice storage pans. Recommendations for preventing problems that could be caused by B. cereus as a result of preparation and storage practices are to (a) cook only small batches of rice at intervals during the day, (b) hold cooked rice at or above 55 C (131 F), (c) cool cooked rice in shallow pans in layers less than 9-cm (3.5 in.) thick and (d) fry rice so that every grain is certain to reach a temperature of at least 74 C (165 F).


2019 ◽  
Vol 3 (5) ◽  
pp. 471-484
Author(s):  
Adeniyi Adeleye ◽  
Mohammed B. Yerima ◽  
Michael E. Nkereuwem ◽  
Victor O. Onokebhagbe ◽  
Peter G. Shiaka ◽  
...  

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