Bacillus cereus Contamination of Seeds and Vegetable Sprouts Grown in a Home Sprouting Kit

Sprouting seeds (alfalfa, mung bean and wheat) were purchased at local health food stores and examined for Bacillus cereus by the official AOAC method. Of 98 units collected, 56 (57%) were positive for B. cereus at levels ranging from 3 to >500 per g. Population levels of B. cereus on sprouts grown from naturally contaminated seeds in a home sprouting kit ranged from a mean of log10 3.72 for alfalfa to 5.39 for wheat; the log10 mean for mung bean sprouts was 4.52. Washing contaminated sprouts for 10 min with warm tap water as recommended by the manufacturer of the sprouting kits reduced the B. cereus count for mung bean sprouts by approximately one log unit but was less effective for wheat sprouts. B. cereus populations large enough to cause food poisoning (>105/g) frequently remained on wheat sprouts even after three wash cycles, and significant numbers of viable B. cereus remained on wheat sprouts even after cooking for 20 min.

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A Simple Spectrofluorometric Method for the Determination of Total Auxins in Mung Bean Sprouts

Journal of Analytical Chemistry ◽

10.1134/s1061934820110155 ◽

2020 ◽

Vol 75 (11) ◽

pp. 1404-1407

Author(s):

Aziguli Yigaimu ◽

Jiahua Chang ◽

Amina Hoji ◽

Turghun Muhammad ◽

Burabiye Yakup ◽

...

Keyword(s):

Mung Bean ◽

Mung Bean Sprouts ◽

Spectrofluorometric Method ◽

Bean Sprouts

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Health Information Provided by Retail Health Food Outlets

Canadian Journal of Gastroenterology ◽

10.1155/2000/878212 ◽

2000 ◽

Vol 14 (9) ◽

pp. 767-771 ◽

Cited By ~ 11

Author(s):

Jaclyn Calder ◽

Robert Issenman ◽

Ruth Cawdron

Keyword(s):

Information Sources ◽

Nutritional Supplements ◽

The Internet ◽

Local Health ◽

Research Assistant ◽

Health Food ◽

Herbal Supplements ◽

Alternative Health ◽

Food Stores ◽

Source Of Information

Alternative health practices have become increasingly popular in recent years. Many patients visit specific complementary practitioners, while others attempt to educate themselves, trusting advice from employees at local health food stores or the Internet. Thirty-two retail health food stores were surveyed on the nature of the information provided by their staff. A research assistant visited the stores and presented as the mother of a child in whom Crohn’s disease had been diagnosed. Seventy-two per cent (23 of 32) of store employees offered advice, such as to take nutritional and herbal supplements. Of the 23 stores where recommendations were made, 15 (65%) based their recommendation on a source of information. Fourteen of the 15 stores using information sources used the same reference book. This had a significant impact on the recommendations; the use of nutritional supplements was favoured. In conclusion, retail health food stores are not as inconsistent as hypothesized, although there are many variances in the types of supplements recommended for the same chronic disease.

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Scanning electron microscopy of native biofilms on mung bean sprouts

Canadian Journal of Microbiology ◽

10.1139/w03-002 ◽

2003 ◽

Vol 49 (1) ◽

pp. 45-50 ◽

Cited By ~ 32

Author(s):

William F Fett ◽

Peter H Cooke

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Food Safety ◽

Vigna Radiata ◽

Mung Bean ◽

Fibrillar Material ◽

Mung Bean Sprouts ◽

Native Microflora ◽

Bean Sprouts ◽

Scanning Electron

Native biofilms present on the adaxial surface of cotyledons of mung bean sprouts (Vigna radiata) were studied by use of scanning electron microscopy. Biofilms were abundant on the cotyledon surfaces and were comprised of rod-shaped bacteria, cocci-shaped bacteria, or yeasts, often with one type of microbe predominant. In contrast to our earlier study of biofilms on green sprouts (alfalfa, clover, broccoli, and sunflower), yeast and cocci were abundant on mung bean. Filamentous fungi were not observed. Sheet-like or fibrillar material (presumably composed of secreted microbial polysaccharides, proteins, lipids, and nucleic acids) fully or partially covered the biofilms. Biofilms up to 5 mm in length were observed, and some biofilms were comprised of more than just a monolayer of microbial cells. Native biofilms on sprout surfaces undoubtedly play an important role in the ecology of plant epiphytic microbes and may also afford protected sites for plant and human bacterial pathogens.Key words: mung bean sprouts, biofilms, native microflora, scanning electron microscopy, food safety.

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Enzymes of Nucleic Acid Metabolism from Mung Bean Sprouts

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)96545-2 ◽

1966 ◽

Vol 241 (12) ◽

pp. 2876-2880 ◽

Cited By ~ 2

Author(s):

Hubert S. Loring ◽

J.E. McLennan ◽

Tom L. Walters

Keyword(s):

Nucleic Acid ◽

Mung Bean ◽

Acid Metabolism ◽

Nucleic Acid Metabolism ◽

Mung Bean Sprouts ◽

Bean Sprouts

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A Nuclease from Mung Bean Sprouts

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)93952-9 ◽

1962 ◽

Vol 237 (2) ◽

pp. 506-511 ◽

Cited By ~ 6

Author(s):

Shan-ching Sung ◽

M. Laskowski

Keyword(s):

Mung Bean ◽

Mung Bean Sprouts ◽

Bean Sprouts

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Effect of putative probiont Enterococcus hirae on the hematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v14n3.1097 ◽

2018 ◽

Vol 14 (3) ◽

pp. 423-428 ◽

Cited By ~ 1

Author(s):

Nur Hidayahanum Hamid ◽

Hassan Mohd Daud ◽

Prapansak Srisapoome ◽

Hasliza Abu Hassim ◽

Md Sabri Mohd Yusoff ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Mung Bean ◽

Hematological Parameters ◽

Clarias Gariepinus ◽

African Catfish ◽

Post Challenge ◽

Control Groups ◽

Blood Samples ◽

Mung Bean Sprouts ◽

Bean Sprouts

Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African catfish, Clarias gariepinus during pre- and post-challenge with aquatic pathogen, Aeromonas hydrophila. The probiotics were previously isolated from vegetable wastes (mung bean sprouts, Vigna radiate and cucumber, Cucumis sativus) which have been fermented for 7 days. The experimental fish (270 tails) with an average weight of 5.13 ± 1.03 g were distributed and divided randomly into i) control (30 tails), fed with commercial diet ii) E1 (30 tails), fed with diets supplemented with 108 CFU/ml of E. faecium isolated from fermented cucumber, iii) E2 (30 tails), fed with 108 CFU/ml of E. faecium isolated from fermented mung bean sprouts. The feeding trial was conducted for 50 days. All experimental groups were then challenged with A. hydrophila (1.5 × 106 CFU/mL) via intraperitoneal injection on day 51. Prior to challenge, blood samples were collected from five fish randomly selected from each group on the day 51 (pre-challenge). After 72 hours of post-challenge, blood samples were again collected from five fish from each groups. The hematological parameters such as total erythrocyte count (RBC), total leucocyte count (WBC), packed cell volume (PCV), hemoglobin (Hb), the derived blood indices of mean corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) were examined. Hematological profiles of pre- and post-challenge infected juvenile catfish were compared with the control groups. The RBC, Hb, WBC, PCV, MCV and MCHC of fish fed with probiotics showed higher significant difference (P<0.05) as compared to control groups during pre- and post-challenge of pathogen. The high level of RBC and WBC during pre- and post-challenge showed the capability of the probiotics to improve the immune response of juvenile African catfish and thus increased the fish disease resistance against A. hydrophila infection. The result suggested that E. faecium could be used effectively as a probiotic in aquaculture.

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