Survival of Large Populations of Listeria monocytogenes on Chicken Breasts Processed Using Moist Heat

1989 ◽  
Vol 52 (6) ◽  
pp. 376-378 ◽  
Author(s):  
MARK A. HARRISON ◽  
SANDRA L. CARPENTER
Keyword(s):  
Growth Rate ◽  
Listeria Monocytogenes ◽  
Heat Treatments ◽  
Microbial Populations ◽  
Heating Method ◽  
Moist Heat ◽  
Three Samples ◽  
Large Populations ◽  
Storage Temperatures

The ability of Listeria monocytogenes to survive and proliferate on chicken processed using a moist heating method was investigated. Chicken breasts were inoculated with 106–107 microorganisms/g, cooked to one of five different cooking temperatures, then either vacuum packaged or wrapped in an oxygen permeable film and stored at 4°C for up to 4 weeks or at 10°C for up to 10 d. Lethality was directly related to the cooking temperatures employed in this study, however survivors were encountered at each of the heat treatments employed. By the fourth week of storage at 4°C, the L. monocytogenes population in all of the samples, except those cooked to 82.2°C increased significantly. In contrast, within the first week of storage at 4°C the population increased in only three samples (73.9°C film overwrap, 65.6°C and 71.1°C vacuum packaged). Storage at 10°C allowed microbial populations in 6 of the 10 treatments to significantly increase within 3 d, with the remaining 4 significantly increasing within 10 d. Differences in packaging influenced the growth rate of L. monocytogenes at both storage temperatures.

Fate of Small Populations of Listeria monocytogenes on Poultry Processed Using Moist Heat

1989 ◽  
Vol 52 (11) ◽  
pp. 768-770 ◽  
Author(s):  
SANDRA L. CARPENTER ◽  
MARK A. HARRISON
Keyword(s):  
Listeria Monocytogenes ◽  
Sampling Period ◽  
Small Populations ◽  
Inoculum Concentration ◽  
Heating Method ◽  
Initial Inoculum ◽  
Moist Heat ◽  
Cooking Process ◽  
Storage Temperatures

The survival of small populations of Listeria monocytogenes on poultry processed using a moist heating method was determined. Various inoculum concentrations (3.2 × 102, 4.8 × 103, and 4.7 × 104) were applied to chicken breasts which were cooked to an internal endpoint temperature of 73.9°C (165°F). After cooking, portions were either vacuum-packaged or wrapped in an oxygen permeable film and stored for up to 4 wk at 4°C or up to 10 d at 10°C. Some Listeria survived the cooking process regardless of the inoculum levels. Significant increases (p<0.05) in the L. monocytogenes population occurred for each inoculum concentration at both storage temperatures within the first sampling period (week 1 for 4°C and day 3 for 10°C). Samples stored at both temperatures were able to re-establish themselves to population levels at or above the initial inoculum. No differences were noted due to packaging.

Effect of Mayonnaise pH and Storage Temperature on the Behavior of Listeria monocytogenes in Ham Salad and Potato Salad†

2005 ◽  
Vol 68 (8) ◽  
pp. 1628-1634 ◽  
Author(s):  
CHENG-AN HWANG
Keyword(s):  
Growth Rate ◽  
Listeria Monocytogenes ◽  
Storage Temperature ◽  
Inactivation Rate ◽  
Consistent Effect ◽  
Cell Counts ◽  
Cooked Ham ◽  
Mathematical Equations ◽  
And Storage ◽  
Storage Temperatures

This study examined and modeled the behavior of Listeria monocytogenes in ham salad and potato salad as affected by the pH of mayonnaise and storage temperature. An eight-strain cocktail of L. monocytogenes was inoculated on the surface of diced cooked ham or potato. The inoculated ham or potato was then mixed with regular mayonnaise (pH 3.8) or mayonnaise that was adjusted with NaOH to pH 4.2 or 4.6. The cell counts of L. monocytogenes in the salads during storage at 4, 8, or 12°C were enumerated and used to model the behavior of L. monocytogenes in ham salad or potato salad. At each of the storage temperatures, L. monocytogenes was able to grow in ham salad, whereas L. monocytogenes was inactivated in potato salad. The growth rate (log CFU per hour) in ham salad or the inactivation rate (log CFU per hour) in potato salad increased as the storage temperature increased. The duration before growth in ham salad or inactivation in potato salad increased as storage temperature decreased. The mayonnaise pH showed no consistent effect on the growth rate or inactivation rate and duration before growth or inactivation occurred. Mathematical equations that described the growth rate or inactivation rate of L. monocytogenes in both salads as a function of mayonnaise pH and storage temperature were generated and shown to be satisfactory in describing the growth rate or inactivation rate of L. monocytogenes in the ham salad or potato salad.

scholarly journals Growth of Listeria monocytogenes in Thawed Frozen Foods

2017 ◽  
Vol 80 (3) ◽  
pp. 447-453 ◽  
Author(s):  
Ai Kataoka ◽  
Hua Wang ◽  
Philip H. Elliott ◽  
Richard C. Whiting ◽  
Melinda M. Hayman
Keyword(s):  
Growth Rate ◽  
Listeria Monocytogenes ◽  
Growth Rates ◽  
Storage Temperature ◽  
Growth Parameters ◽  
Quadratic Model ◽  
Lag Phase ◽  
Frozen Foods ◽  
Phase Duration ◽  
Primary Model

ABSTRACT The growth characteristics of Listeria monocytogenes inoculated onto frozen foods (corn, green peas, crabmeat, and shrimp) and thawed by being stored at 4, 8, 12, and 20°C were investigated. The growth parameters, lag-phase duration (LPD) and exponential growth rate (EGR), were determined by using a two-phase linear growth model as a primary model and a square root model for EGR and a quadratic model for LPD as secondary models, based on the growth data. The EGR model predictions were compared with growth rates obtained from the USDA Pathogen Modeling Program, calculated with similar pH, salt percentage, and NaNO2 parameters, at all storage temperatures. The results showed that L. monocytogenes grew well in all food types, with the growth rate increasing with storage temperature. Predicted EGRs for all food types demonstrated the significance of storage temperature and similar growth rates among four food types. The predicted EGRs showed slightly slower rate compared with the values from the U.S. Department of Agriculture Pathogen Modeling Program. LPD could not be accurately predicted, possibly because there were not enough sampling points. These data established by using real food samples demonstrated that L. monocytogenes can initiate growth without a prolonged lag phase even at refrigeration temperature (4°C), and the predictive models derived from this study can be useful for developing proper handling guidelines for thawed frozen foods during production and storage.

INFLUENCE OF MOIST-HEAT TREATMENTS OF PEANUTS ON PEANUT PASTE CHARACTERISTICS

1974 ◽  
Vol 39 (3) ◽  
pp. 494-497 ◽  
Author(s):  
K. H. McWATTERS ◽  
E. K. HEATON
Keyword(s):  
Heat Treatments ◽  
Moist Heat

Growth Modeling of Listeria monocytogenes in Pasteurized Liquid Egg

2013 ◽  
Vol 76 (9) ◽  
pp. 1549-1556 ◽  
Author(s):  
MIHO OHKOCHI ◽  
SHIGENOBU KOSEKI ◽  
MASAAKI KUNOU ◽  
KATSUAKI SUGIURA ◽  
HIROKAZU TSUBONE
Keyword(s):  
Listeria Monocytogenes ◽  
Specific Growth ◽  
Storage Temperature ◽  
Growth Parameters ◽  
Root Model ◽  
Natural Flora ◽  
Mean Square Errors ◽  
Kinetics Of ◽  
Storage Temperatures ◽  
Maximum Population Density

The growth kinetics of Listeria monocytogenes and natural flora in commercially produced pasteurized liquid egg was examined at 4.1 to 19.4°C, and a growth simulation model that can estimate the range of the number of L. monocytogenes bacteria was developed. The experimental kinetic data were fitted to the Baranyi model, and growth parameters, such as maximum specific growth rate (μmax), maximum population density (Nmax), and lag time (λ), were estimated. As a result of estimating these parameters, we found that L. monocytogenes can grow without spoilage below 12.2°C, and we then focused on storage temperatures below 12.2°C in developing our secondary models. The temperature dependency of the μmax was described by Ratkowsky's square root model. The Nmax of L. monocytogenes was modeled as a function of temperature, because the Nmax of L. monocytogenes decreased as storage temperature increased. A tertiary model of L. monocytogenes was developed using the Baranyi model and μmax and Nmax secondary models. The ranges of the numbers of L. monocytogenes bacteria were simulated using Monte Carlo simulations with an assumption that these parameters have variations that follow a normal distribution. Predictive simulations under both constant and fluctuating temperature conditions demonstrated a high accuracy, represented by root mean square errors of 0.44 and 0.34, respectively. The predicted ranges also seemed to show a reasonably good estimation, with 55.8 and 51.5% of observed values falling into the prediction range of the 25th to 75th percentile, respectively. These results suggest that the model developed here can be used to estimate the kinetics and range of L. monocytogenes growth in pasteurized liquid egg under refrigerated temperature.

scholarly journals Verification of prediction of growth of Listeria monocytogenes micro-organism in chicken meat

2000 ◽  
Vol 18 (No. 5) ◽  
pp. 183-186
Author(s):  
A. Landfeld ◽  
M. Karpíšková R Houška ◽  
K. Kýhos ◽  
P. Novotná
Keyword(s):  
Listeria Monocytogenes ◽  
Incubation Temperature ◽  
Chicken Meat ◽  
Raw Material ◽  
Plate Count ◽  
Raw Meat ◽  
Total Plate Count ◽  
Measured Water ◽  
Number Of Bacteria ◽  
Storage Temperatures

Raw chicken meat was comminuted and homogenised. There were measured water activity and pH (aw = 1 for temperature 25°C, pH = 5.8 for temperature 8°C). Input raw material was investigated for the presence of Listeria monocytogenes (negative) and the raw meat was inoculated by Listeria monocytogenes CCM 4699. Number of Listeria monocytogenes, total plate count and number of coliforms were determined in the range 0–7 days by bacteriological survey for the storage temperatures 4.9, 7 and 8.3°C. The increase of Listeria monocytogenes counts has been registered for temperature 4.9°C about log 1.5 CFU/g after 6 days, for temperatures 7 and 8.3°C about 2 log CFU/g (regarding to the starting counts). The prediction for listeria growth with the aid of Food MicroModel was also made. The best agreement between the experimentally analysed number of bacteria and prediction was received for the lowest incubation temperature 4.9°C.

Effect of Single or Sequential Hot Water and Lactic Acid Decontamination Treatments on the Survival and Growth of Listeria monocytogenes and Spoilage Microflora during Aerobic Storage of Fresh Beef at 4, 10, and 25°C

2004 ◽  
Vol 67 (12) ◽  
pp. 2703-2711 ◽  
Author(s):  
KONSTANTINOS P. KOUTSOUMANIS ◽  
LAURA V. ASHTON ◽  
IFIGENIA GEORNARAS ◽  
KEITH E. BELK ◽  
JOHN A. SCANGA ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Hot Water ◽  
Microbial Profile ◽  
Spoilage Bacteria ◽  
Brochothrix Thermosphacta ◽  
Survival And Growth ◽  
Spoilage Microflora ◽  
Storage Temperatures

The survival and growth of Listeria monocytogenes and spoilage microflora during storage of fresh beef subjected to different decontamination treatments was studied. Fresh beef inoculated with a five-strain mixture of L. monocytogenes (5.18 log CFU/cm2) was left untreated (control) or was immersed (30 s) in hot water (HW; 75°C), 2% lactic acid (LA; 55°C), hot water followed by lactic acid (HW-LA), or lactic acid followed by hot water (LA-HW) and then stored aerobically at 4, 10, and 25°C for 25, 17, and 5 days, respectively. Initial populations of L. monocytogenes were reduced by 0.82 (HW), 1.43 (LA), 2.73 (HW-LA), and 2.68 (LA-HW) log CFU/cm2. During storage, the pathogen grew at higher rates in HW than in control samples at all storage temperatures. Acid decontamination treatments (LA, HW-LA, and LA-HW) resulted in a weaker inhibition of L. monocytogenes (P < 0.05) at 25°C than at 4 and 10°C. In general, the order of effectiveness of treatments was HW-LA > LA > LA-HW > HW > control at all storage temperatures tested. In untreated samples, the spoilage microflora was dominated by pseudomonads, while lactic acid bacteria, Enterobacteriaceae, and yeasts remained at lower concentrations during storage. Brochothrix thermosphacta was detected periodically in only a limited number of samples. Although decontamination with HW did not affect the above spoilage microbial profile, acid treatments shifted the predominant microflora in the direction of yeasts and gram-positive bacteria (lactic acid bacteria). Overall, the results of the present study indicate that decontamination with LA and combinations of LA and HW could limit growth of L. monocytogenes and inhibit pseudomonads, which are the main spoilage bacteria of fresh beef stored under aerobic conditions. However, to optimize the efficacy of such treatments, they must be applied in the appropriate sequence and followed by effective temperature control.

Fate of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella typhimurium during Preparation and Storage of Beef Jerky

1996 ◽  
Vol 59 (12) ◽  
pp. 1336-1338 ◽  
Author(s):  
JUDY A. HARRISON ◽  
MARK A. HARRISON
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Microbial Populations ◽  
Escherichia Coli O157 ◽  
Beef Jerky ◽  
Drying Up ◽  
Moisture Conditions ◽  
And Storage

The fate of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella typhimurium during preparation and storage of beef jerky was determined. Control strips and one-half of the inoculated beef loin strips were marinated at 4°C overnight and dried at 60°C (140°F) for 10h. The remaining half of the inoculated samples were heated in marinade to 71.1°C (160°F). Strips were dried at 60°C (140°F) for 10 h. Microbial populations were determined at intervals during drying up to 10 h and also from samples stored at 25°C for 8 weeks at various moisture levels. In general, L. monocytogenes was more resistant to the treatments. After 3 h of drying, populations on the unheated, inoculated samples were reduced by 3.3, 1.8 and 3.1 log units, respectively, and all three were reduced by 5.5 to 6.0 log units after 10h. Reduction of the three populations on strips that were cooked prior to drying was 4.5 to 5.5 log units immediately after cooking. The populations decreased to undetectable levels after 10 h of drying. None of the three pathogens were detected on the controls. After 8 weeks of storage none of the pathogens were detected, indicating that they were unable to recover under the moisture conditions during storage.

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