Verification of the level of Microbiological Control for the Slaughter and Cooling Processes of Beef Carcass Production at a High-line-Speed Abattoir

1997 ◽  
Vol 60 (12) ◽  
pp. 1509-1514 ◽  
Author(s):  
KLAUS W. F. JERICHO ◽  
GERALD C. KOZUB ◽  
VICTOR P. J. GANNON ◽  
ELIZABETH J. GOLSTEYN THOMAS ◽  
ROBIN K. KING ◽  
...  
Keyword(s):  
Control Point ◽  
Most Probable Number ◽  
Large Sample Size ◽  
Probable Number ◽  
Bacterial Counts ◽  
E Coli ◽  
Critical Control Point ◽  
Line Speed ◽  
High Line ◽  
Pooled Samples

Methods are described which were used to verify the microbiological adequacy of the processes of production and chilling of carcasses at a high-line-speed abattoir. Ten excision samples (5 by 5 by 0.2 cm) were taken from each of 16 to 20 carcasses for each evaluation of these processes. Twelve monthly evaluations were made for the slaughter of steers, heifers, and cows and additional evaluations for each of the slaughter of cows and the chill process of carcasses. The ranges of the estimated mean log10 most probable number of growth units per square centimeter (LMPN, for 236 carcasses) and Escherichia coli per square centimeter (LEC, for 240 carcasses) enumerated by hydrophobic-grid membrane filter technology for the 12 monthly evaluations of the slaughter floor were 1.11 to 1.62 (LMPN) for single samples and 0.20 to 0.65 (LEC) for pooled samples. Based on a published advisory scale for the slaughter floor the aerobic bacterial counts reflect a cleanliness level of ”excellent” to “good.” For single evaluations of cow carcasses at the end of slaughter and of chilled carcasses the mean LMPN was 1.78 (“good”) and 1.40 respectively. From pooled samples of each of the 236 steer, heifer, and cow carcasses the pathogen E. coli O157:H7 was identified by polymerase chain reaction on one carcass whereas Listeria monocytogenes was identified on 14 carcasses. Verocytoxigenic E. coli (6 isolates) and L. monocytogenes were not isolated from the same carcasses. These low isolation rates dictate a large sample size and therefore these pathogens are excluded from use to routinely verify the workings of hazard analyses and critical control point (HACCP) systems for beef slaughter processes in Alberta. Alternatively the use of aerobic bacterial counts to directly measure cleanliness or of E. coli counts to indirectly measure fecal contamination appears to be more practical than the use of specific pathogen counts for regulatory agencies to verify the workings of quality control programs, including HACCP systems.

Microbiological Testing of Raw, Boxed Beef in the Context of Hazard Analysis Critical Control Point at a High-Line-Speed Abattoir

2000 ◽  
Vol 63 (12) ◽  
pp. 1681-1686 ◽  
Author(s):  
K. W. F. JERICHO ◽  
G. C. KOZUB ◽  
V. P. J. GANNON ◽  
C. M. TAYLOR
Keyword(s):  
Cold Storage ◽  
Hazard Analysis ◽  
Control Point ◽  
Aerobic Bacteria ◽  
Cooling Process ◽  
E Coli ◽  
Critical Control Point ◽  
Line Speed ◽  
High Line ◽  
Before And After

The efficacy of cold storage of raw, bagged, boxed beef was assessed microbiologically at a high-line-speed abattoir (270 carcasses per h). At the time of this study, plant management was in the process of creating a hazard analysis critical control point plan for all processes. Aerobic bacteria, coliforms, and type 1 Escherichia coli were enumerated (5 by 5-cm excision samples, hydrophobic grid membrane filter technology) before and after cold storage of this final product produced at six fabrication tables. In addition, the temperature-function integration technique (TFIT) was used to calculate the potential number of generations of E. coli during the first 24 or 48 h of storage of the boxed beef. Based on the temperature histories (total of 60 boxes, resulting from 12 product cuts, five boxes from each of two fabrication tables on each of 6 sampling days, and six types of fabrication tables), TFIT did not predict any growth of E. coli (with or without lag) for the test period. This was verified by E. coli mean log10 values of 0.65 to 0.42 cm2 (P > 0.05) determined by culture before and after the cooling process, respectively. Counts of aerobic bacteria and coliforms were significantly reduced (P < 0.001 and P < 0.05, respectively) during the initial period of the cooling process. There were significant microbiological differences (P < 0.05) between table-cut units.

Verifying Apple Cider Plant Sanitation and Hazard Analysis Critical Control Point Programs: Choice of Indicator Bacteria and Testing Methods

1999 ◽  
Vol 62 (8) ◽  
pp. 887-893 ◽  
Author(s):  
MEGAN M. LANG ◽  
STEVEN C. INGHAM ◽  
BARBARA H. INGHAM
Keyword(s):  
Quantitative Methods ◽  
Hazard Analysis ◽  
Control Point ◽  
Most Probable Number ◽  
Good Survival ◽  
Processing Plant ◽  
Probable Number ◽  
Apple Cider ◽  
E Coli ◽  
Critical Control Point

The objectives of this study were (i) to evaluate the survival of coliforms, Escherichia coli, and enterococci in refrigerated apple cider; (ii) to develop simple and inexpensive presumptive methods for detection of these bacteria; (iii) to perform a field survey to determine the prevalence of these bacteria on apples and in apple cider; and (iv) based on our results, to recommend the most useful of these three indicator groups for use in verifying apple cider processing plant sanitation and hazard analysis critical control point (HACCP) programs. Eight of 10 coliform strains (5 E. coli, 1 Enterobacter aerogenes, and 2 Klebsiella spp.) inoculated into preservative-free apple cider (pH 3.4, 13.3° Brix) survived well at 4°C for 6 days (≤3.0 log10 CFU/ml decrease). Of 21 enterococci strains (Enterococcus faecalis, E. faecium, and E. durans), only 2 E. durans and 3 E. faecium strains survived well. Simple broth-based colorimetric methods were developed that detected the presence of ∼10 cells of coliforms or enterococci. In three field studies, samples of unwashed apples (drops and picked), washed apples, and freshly pressed cider were presumptively analyzed for total coliforms, E. coli, and enterococci using qualitative and/or quantitative methods. Drop apples were more likely than picked apples to be contaminated with E. coli (26.7% vs. 0%) and enterococci (20% vs. 0%). Washing had little effect on coliform populations and in one field study was associated with increased numbers. Total coliform populations in cider ranged from <1 CFU/ml to >738 most probable number/ml, depending on the enumeration method used and the sample origin. E. coli was not recovered from washed apples or cider, but enterococci were present on 13% of washed apple samples. The qualitative coliform method successfully detected these bacteria on apples and in cider. Based on its exclusively fecal origin, good survival in apple cider, and association with drop apples, we conclude that E. coli is the most useful organism for verifying apple cider sanitation and HACCP programs.

Microbial Evaluation of Spanish Potato Omelette and Cooked Meat Samples in University Restaurants

2000 ◽  
Vol 63 (9) ◽  
pp. 1273-1276 ◽  
Author(s):  
J. M. SORIANO ◽  
H. RICO ◽  
J. C. MOLTÓ ◽  
J. MAÑES
Keyword(s):  
Control Point ◽  
Most Probable Number ◽  
Probable Number ◽  
Pork Sausage ◽  
E Coli ◽  
Meat Samples ◽  
Cooked Meat ◽  
Group D ◽  
Coagulase Positive Staphylococci ◽  
Lancefield Group

The focus of this study was to evaluate the microbial quality of Spanish potato omelette and cooked meat samples including pork loin, chicken croquettes, long pork sausage, chicken breast, and meatballs from University restaurants. Microbiological analyses of Spanish potato omelette and cooked meat samples resulted in aerobic plate counts from <1.00 to 2.90 and from <1.00 to 6.04 log10 CFU g−1, respectively. Total coliforms ranged from <3 to 43 most probable number (MPN) g−1 and from <3 to >2,400 MPN g−1 for Spanish potato omelette and meat products, respectively. Escherichia coli, coagulase-positive staphylococci, and Lancefield group-D streptococci were detected in 1.7%, 3.5%, and 12.9% of Spanish potato omelette samples, respectively. For cooked meat samples, 8.8%, 7.6%, and 24.6% contained E. coli, coagulase-positive staphylococci, and Lancefield group-D streptococci, respectively. E. coli O157:H7, Salmonella spp., and Shigella spp. were not detected. Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter freundii, Enterobacter cloacae, and Serratia spp. were isolated from Spanish potato omelette samples. For cooked meat samples, C. freundii, E. cloacae, and Aeromonas hydrophila were detected. The results suggest that some handling practices should require more attention, and as a consequence, a hazard analysis and critical control point program should be developed and implemented.

scholarly journals Effectiveness of the Thermal Treatments Used for Curd Stretching in the Inactivation of Shiga Toxin-Producing O157 and O26Escherichia coli

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
M. Trevisani ◽  
A. Valero ◽  
R. Mancusi
Keyword(s):  
Dynamic Model ◽  
Shiga Toxin ◽  
Variable Temperature ◽  
Control Point ◽  
Hot Water ◽  
Most Probable Number ◽  
Heating Process ◽  
Thermal Treatments ◽  
Probable Number ◽  
Critical Control Point

The kneading treatment of the fresh curd in hot water is a critical control point in the manufacturing of mozzarella. Factors such as the ratio between hot water and curd mass, the rheological properties, and the mixing and kneading activity affect the processing time and the internal temperature of the curd. The aim of this study was to investigate the effect of thermal treatments on the fate of Shiga toxin-producingEscherichia coli(STEC). Nine curd samples (weight 160–270 g) were artificially contaminated with O157 or O26 STEC and stretched in hot water (90–95°C) for 5–10 min. Depending on the heating process and spinning, different nonisothermal profiles were recorded. Observed reductions of O157 and O26 STEC varied between 1.01 and more than 5.38 log⁡MPN(Most Probable Number)/g at the end of the temperature treatments. Further, nonisothermal log-linear tail models were developed to compare observed reductions for O157 and O26 VTEC under variable temperature conditions. Results obtained showed that the comparison of predictions provided by the dynamic model with observations described well the linear inactivation pattern since nonsignificant differences were denoted at all profiles tested. The dynamic model developed can be useful to evaluate the effectiveness of the thermal treatments used in the manufacturing of mozzarella in the inactivation of STEC.

A Rapid Most-Probable-Number–Based Enzyme-Linked Immunosorbent Assay for the Detection and Enumeration of Salmonella Typhimurium in Poultry Wastewater

2003 ◽  
Vol 66 (12) ◽  
pp. 2302-2306 ◽  
Author(s):  
C. GOODRIDGE ◽  
L. GOODRIDGE ◽  
D. GOTTFRIED ◽  
P. EDMONDS ◽  
J. C. WYVILL
Keyword(s):  
Salmonella Typhimurium ◽  
Immunosorbent Assay ◽  
Hazard Analysis ◽  
Control Point ◽  
Most Probable Number ◽  
Enzyme Linked Immunosorbent Assay ◽  
Probable Number ◽  
Critical Control Point ◽  
Critical Components ◽  
Poultry Wastewater

The rapid and accurate detection and enumeration of low levels of Salmonella Typhimurium in food processing facilities are critical components of an effective hazard analysis critical control point program. The objective of this study was to develop a rapid (8 h) most probable number (MPN)–enzyme-linked immunosorbent assay (ELISA) for the detection and enumeration of Salmonella Typhimurium in wastewater. The specific objectives were to (i) characterize poly- and monoclonal Salmonella Typhimurium–specific antibodies in order to select the most specific and sensitive antibody for Salmonella Typhimurium detection, and (ii) validate the MPN assay through a correlation between the 8-h MPN-ELISA and the traditional 48-h Salmonella Typhimurium MPN method in poultry scald water. Poultry scald water samples were spiked with 10 and 50 CFU/ml of Salmonella Typhimurium. The traditional MPN method used a 48-h enrichment period followed by an analysis, while the MPN-ELISA used a 5-h enrichment period followed by a 3-h ELISA analysis. No differences (P < 0.05) were found between the traditional MPN and the MPN-ELISA, indicating the promise of the MPN-ELISA for the rapid detection and enumeration of Salmonella Typhimurium within an 8-h shift. This abbreviated assay will permit increased product sampling and more rapid movement of food between production and processing, resulting in reduced spoilage and quality losses.

scholarly journals Efficieny of Phage Intervention Against Salmonella in Meat and Poultry Processing

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
B. De Vegt ◽  
S. Sirdesai ◽  
R. Peterson ◽  
M. Pinheiro ◽  
W. Nuboer ◽  
...  
Keyword(s):  
Dwell Time ◽  
Pathogenic Bacteria ◽  
Hold Time ◽  
Control Point ◽  
Bacterial Attachment ◽  
Most Probable Number ◽  
Water Control ◽  
Probable Number ◽  
Critical Control Point ◽  
Consumer Safety

ObjectivesPathogen Reduction, Hazard Analysis, and Critical Control Point systems final rule mandates establishments to seek and adopt antimicrobial interventions that can help in reducing the prevalence and most probable number of Salmonella in their meat and poultry products. Bacteriophages can aid in this challenge, as they can invade and kill specific target pathogenic bacteria on food products. Effective kill by phages relies on the appropriate phage application technique. Correct dose, good distribution on the food surface area, and adequate dwell time are key factors which influence phage-bacteria contact and thereby phage efficacy. This study determined the efficacy of a commercially available phage product, PhageGuard S consisting of 2 phages, FO1a, and S16. Different pick up levels, blend and hold times (chosen based on regulatory restriction and process limitations), as well as spray versus dip treatment methods were tested.Materials and MethodsOvernight culture streptomycin resistant Salmonella enterica enterica Enteritidis C (Se13) was diluted and inoculated at a concentration of 2×104 CFU/cm2 or CFU/g on parts of chicken fillet and held for 10 min for bacterial attachment (duplicate samples per time point). Subsequently, contaminated parts were spray treated with one phage concentration (108 Plaque Forming Units/g) at 0.5%, 1% or 3% pick up (v/w) or water (control) and blended for 5, 10, and 20 min before immediate grinding and retrieval of bacteria (latter blend time sample was held for 24 h before grind). Another set of contaminated fillet parts were treated by dipping in 5% phage solution (at 1% pick up, 108 PFU/g) and held for 1, 5, 10, and 20 min, and 1 and 24 h at 40°F (4°C) before retrieval of bacteria. Enumeration of bacteria was done on selective agar plates and reductions were calculated relative to water treated control.ResultsThe application of phages 108 PFU/g via spray on chicken parts at 3% pick up and 20 min blend time resulted in 0.9 log10 CFU/g log reduction of Salmonella. Additional hold time of 24 h before grind resulted in 1.1- 1.2 log10 CFU/g kill at lower and higher pick up of 0.5% and 3%. Dip treatment resulted higher Salmonella reduction of 1.2 log10 CFU/cm2 within 5 min of 108 PFU/cm2 phage application and up to 2.3 log10 CFU/cm2 log10 reduction when held for 24 h. Overall, the spray technique, showed a dose response effect where increasing pick up and blend time resulted in an increasing Salmonella kill in ground product. However, the dip technique resulted in more effective Salmonella kill in shorter dwell time. All values are mean value of two individual experiments.ConclusionThe above results indicate that the commercially available phage solution, PhageGuard S, either via spray or dip method reduces Salmonella contamination on meat and poultry parts by 1.2 to 2.3 log10, respectively. Thereby is an effective intervention in reducing risks and allowing for increase in consumer safety. Dip technique works better than spray due to better distribution on meat surface. Longer hold and/or blend time after phage treatment results in more kill.

Aerobiology of a High-Line Speed Cattle Abattoir

2000 ◽  
Vol 63 (11) ◽  
pp. 1523-1528 ◽  
Author(s):  
K. W. F. JERICHO ◽  
J. HO ◽  
G. C. KOZUB
Keyword(s):  
Hazard Analysis ◽  
Strong Association ◽  
Control Point ◽  
Viable Count ◽  
The Other ◽  
Critical Control Point ◽  
Line Speed ◽  
Air Sampler ◽  
High Line ◽  
Canadian Food Inspection Agency

The operation of the high-line speed cattle abattoir studied follows a plant-created hazard analysis and critical control point (HACCP) plan that is recognized by the Canadian Food Inspection Agency. Measurement of bioaerosols is not a part of this plan. In this study CFUs in air of selected abattoir processes were enumerated after impinging air onto tryptic soy agar plates with a slit air sampler for 10 to 20 min. The total viable count (TVC) per liter of air was calculated for each sample following incubation at 30°C for 24 h. Monthly samples were collected on the hide removal floor and the carcass dressing floor from March 1998 to April 1999. Mud tag, dirt, and wetness of incoming hides were scored subjectively on the hide removal floor. The other processes were sampled in 3 separate months. The TVC at two locations on the hide removal floor (center of hide removal floor [CHF] and top of hide puller [THP]) had a strong association to each other (r = 0.84; P < 0.001). The mean TVC at the CHF and THP was 10.0 and 11.5, respectively, and the TVC for individual samples ranged from 2 to 42 at these locations. The TVC means for all the other processes ranged from 0.01 to 0.7. Tag and TVC on the hide removal floor had a different seasonal distribution with TVC being highest in the warm months (April to October 1998) and lowest for November to April 1999. No significant relations between TVC and the dirt and wetness variables were evident for the CHF and THP locations on the hide removal floor. It was concluded that the control of aerosols in the hide removal floor should be treated as a critical control point in the HACCP plan.

scholarly journals Lessons from a large outbreak of Escherichia coli O157[ratio ]H7 infections: insights into the infectious dose and method of widespread contamination of hamburger patties

1999 ◽  
Vol 122 (2) ◽  
pp. 185-192 ◽  
Author(s):  
J. TUTTLE ◽  
T. GOMEZ ◽  
M. P. DOYLE ◽  
J. G. WELLS ◽  
T. ZHAO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ground Beef ◽  
Most Probable Number ◽  
Strong Argument ◽  
Probable Number ◽  
Infectious Dose ◽  
E Coli ◽  
Beef Patties ◽  
Large Outbreak ◽  
Microbiological Testing

Between November 1992 and February 1993, a large outbreak of Escherichia coli O157[ratio ]H7 infections occurred in the western USA and was associated with eating ground beef patties at restaurants of one fast-food chain. Restaurants that were epidemiologically linked with cases served patties produced on two consecutive dates; cultures of recalled ground beef patties produced on those dates yielded E. coli O157[ratio ]H7 strains indistinguishable from those isolated from patients, confirming the vehicle of illness. Seventy-six ground beef patty samples were cultured quantitatively for E. coli O157[ratio ]H7. The median most probable number of organisms was 1·5 per gram (range, <0·3–15) or 67·5 organisms per patty (range, <13·5–675). Correlation of the presence of E. coli O157[ratio ]H7 with other bacterial indicators yielded a significant association between coliform count and the presence of E. coli O157[ratio ]H7 (P=0·04). A meat traceback to investigate possible sources of contamination revealed cattle were probably initially colonized with E. coli O157[ratio ]H7, and that their slaughter caused surface contamination of meat, which once combined with meat from other sources, resulted in a large number of contaminated ground beef patties. Microbiological testing of meat from lots consumed by persons who became ill was suggestive of an infectious dose for E. coli O157[ratio ]H7 of fewer than 700 organisms. These findings present a strong argument for enforcing zero tolerance for this organism in processed food and for markedly decreasing contamination of raw ground beef. Process controls that incorporate microbiological testing of meat may assist these efforts.

scholarly journals Mechanically Ventilated Broiler Sheds: a Possible Source of Aerosolized Salmonella, Campylobacter, and Escherichia coli

2009 ◽  
Vol 75 (23) ◽  
pp. 7417-7425 ◽  
Author(s):  
H. N. Chinivasagam ◽  
T. Tran ◽  
L. Maddock ◽  
A. Gale ◽  
P. J. Blackall
Keyword(s):  
Escherichia Coli ◽  
Most Probable Number ◽  
Production Cycle ◽  
Airborne Bacteria ◽  
Probable Number ◽  
Mechanically Ventilated ◽  
The Public ◽  
E Coli ◽  
Poultry Farms ◽  
Low Levels

ABSTRACT This study assessed the levels of two key pathogens, Salmonella and Campylobacter, along with the indicator organism Escherichia coli in aerosols within and outside poultry sheds. The study ranged over a 3-year period on four poultry farms and consisted of six trials across the boiler production cycle of around 55 days. Weekly testing of litter and aerosols was carried out through the cycle. A key point that emerged is that the levels of airborne bacteria are linked to the levels of these bacteria in litter. This hypothesis was demonstrated by E. coli. The typical levels of E. coli in litter were ∼108 CFU g−1 and, as a consequence, were in the range of 102 to 104 CFU m−3 in aerosols, both inside and outside the shed. The external levels were always lower than the internal levels. Salmonella was only present intermittently in litter and at lower levels (103 to 105 most probable number [MPN] g−1) and consequently present only intermittently and at low levels in air inside (range of 0.65 to 4.4 MPN m−3) and once outside (2.3 MPN m−3). The Salmonella serovars isolated in litter were generally also isolated from aerosols and dust, with the Salmonella serovars Chester and Sofia being the dominant serovars across these interfaces. Campylobacter was detected late in the production cycle, in litter at levels of around 107 MPN g−1. Campylobacter was detected only once inside the shed and then at low levels of 2.2 MPN m−3. Thus, the public health risk from these organisms in poultry environments via the aerosol pathway is minimal.

Reduction of Microorganisms on Citrus Fruit Surfaces during Packinghouse Processing

1998 ◽  
Vol 61 (7) ◽  
pp. 903-906 ◽  
Author(s):  
STEVEN PAO ◽  
G. ELDON BROWN
Keyword(s):  
Test Organism ◽  
Citrus Fruit ◽  
Microbial Populations ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Plate Counts ◽  
High Level ◽  
Inoculation Study ◽  
Fruit Surface

Citrus fruit surface microbial populations were evaluated following various packingline processes of seven Florida commercial packinghouses. At each packinghouse, six fruits (oranges or tangerines) were collected at each of four sampling points. The sampling was conducted in duplicate; thus, 336 fruit were evaluated during this survey. Average aerobic plate counts and yeast and mold counts on fruit surfaces before washing were about 4.0 log CFU/cm2 and 3.3 log CFU/cm2, respectively, and were reduced to 2.1 log CFU/cm2 and 1.3 log CFU/cm2, respectively, by packinghouse processing. Waxing alone reduced the average fruit surface aerobic plate counts and coliform counts from 3.7 log CFU/cm2 and 35.2 most probable number (MPN)/cm2, respectively, to 2.6 log CFU/cm2 and 1.4 MPN/cm2. No Escherichia coli was recovered from fruit at the end of packinghouse processing, and no salmonellae were found on fruit during the entire processing. In an inoculation study to test the effect of packinghouse processes, test organism E. coli was applied to fruit to achieve a high level (4.8 log CFU/cm2) of contamination. The average E. coli count was reduced about 2.4 log cycles by washing and rinsing with potable water (40 psi, 25 °C) for about 30 s. The combination of washing and waxing significantly reduced the inoculated level of E. coli from 4.8 to 1.4 log CFU/cm2.

Sign in / Sign up

Export Citation Format

Share Document