Influence of Poultry Carcass Skin Sample Site on the Effectiveness of Trisodium Phosphate against Listeria monocytogenes

2002 ◽  
Vol 65 (5) ◽  
pp. 853-856 ◽  
Author(s):  
ROSA CAPITA ◽  
CARLOS ALONSO-CALLEJA ◽  
ROBERTO RODRÍGUEZ-PÉREZ ◽  
BENITO MORENO ◽  
MARÍA del CAMINO GARCÍA-FERNÁNDEZ
Keyword(s):  
Listeria Monocytogenes ◽  
Tap Water ◽  
Sampling Site ◽  
Trisodium Phosphate ◽  
Water Control ◽  
Skin Sample ◽  
Sample Site ◽  
Ph Values ◽  
Dorsal Area ◽  
Poultry Carcass

The aim of this study was to determine the influence of skin sample site on the efficacy of trisodium phosphate (TSP) solutions in reducing Listeria monocytogenes populations on chicken carcasses during refrigerated storage. Chicken skin samples from the legs, the breasts, and the dorsal area inoculated with L. monocytogenes (108 CFU/ml) were dipped for 15 min in sterile tap water (control) or in 8, 10, or 12% TSP. L. monocytogenes counts and surface pH values were determined after 0, 1, 3, and 5 days of storage at 2°C. For all sampling times and TSP concentrations, the reductions in L. monocytogenes numbers in breast skin were significantly larger (P < 0.05) than those in leg skin or dorsal skin. No significant differences were found in pH values as an effect of skin site. Our results suggest that skin sampling site is an important factor that needs to be considered when decontamination protocols are developed for poultry carcasses with the TSP treatment.

Note. Effect of trisodium phosphate on mesophilic and psychrotrophic bacterial flora attached to the skin of chicken carcasses during refrigerated storage Nota. Efecto del fosfato trisódico en los microorganismos mesófilos y psicrotrofos presentes en la piel de canales de pollo durante su almacenamiento en refrigeración

2000 ◽  
Vol 6 (4) ◽  
pp. 345-350 ◽  
Author(s):  
R. Capita ◽  
C. Alonso-Calleja ◽  
M.T. García Arias ◽  
B. Moreno ◽  
M.C. García-Fernández
Keyword(s):  
Tap Water ◽  
Bacterial Flora ◽  
Trisodium Phosphate ◽  
Refrigerated Storage ◽  
Water Control ◽  
Bacterial Populations ◽  
Ph Values ◽  
Popula Tions ◽  
Chicken Skin ◽  
Plate Counts

The potential for using trisodium phosphate (TSP) to reduce mesophilic and psychrotrophic popula tions on the skin of chicken carcasses was explored. Skin samples were immersed in sterile tap water (control) or an 8%, 10% or 12% solution of TSP at 20 °C for 15 min. Surface pH values and mesophilic and psychrotrophic plate counts were determined after 0, 1, 3 and 5 days of storage at 2° C. After washing, bacterial populations were significantly smaller in the samples treated with TSP than in the controls. The concentration of the TSP solution was a significant factor in reducing the populations of the bacteria on chicken skin. Before storage, the reduction in the presence of bacteria achieved in treated samples with respect to controls ranged between 0.95 log10 cycles and 1.78 log10 cycles in the case of mesophilic microorganisms, and 0.92 log10 cycles and 1.94 log10 cycles in the case of psychrotrophic strains. These differences between the concentrations of bacteria in samples immersed in water and those treated with TSP increased over time, ranging from 2.35 log 10 cycles to 3.08 log10 cycles (mesophilic microorganisms), and from 2.79 log10 cycles to 4.09 log10 cycles (psychrotrophic microorganisms) on day 5 of storage. The pH of the skin remained more or less constant throughout the study period, ranging between 8 and 9 in skin treated with TSP, depending on the concentration, while it was two units lower in the control samples.

Effectiveness of Trisodium Phosphate against Listeria monocytogenes on Excised and Nonexcised Chicken Skin

2003 ◽  
Vol 66 (1) ◽  
pp. 61-64 ◽  
Author(s):  
ROSA CAPITA ◽  
CARLOS ALONSO-CALLEJA ◽  
MIGUEL PRIETO ◽  
MARÍA del CAMINO GARCÍA-FERNÁNDEZ ◽  
BENITO MORENO
Keyword(s):  
Listeria Monocytogenes ◽  
Tap Water ◽  
Trisodium Phosphate ◽  
Sample Type ◽  
Refrigerated Storage ◽  
Water Control ◽  
Chicken Skin ◽  
Storage Times ◽  
And Storage ◽  
Skin Samples

The influence of sample type (i.e., excised versus nonexcised chicken skin) on the efficiency of trisodium phosphate (TSP) solutions in reducing Listeria monocytogenes populations and inhibiting their growth during refrigerated storage was studied. Whole chicken legs and excised chicken leg skin fragments inoculated with 108 CFU of L. monocytogenes per ml were dipped for 15 min in sterile tap water (control) or in a solution containing 8, 10, or 12% TSP. L. monocytogenes counts were determined after 0, 1, 3, and 5 days of refrigerated storage (2°C). The decontamination effect of TSP was greater for excised skin than for whole legs. Microbial differences between control and TSP-treated samples were significantly larger for excised skin than for whole legs for 9 (75%) of 12 tested combinations of TSP concentrations and storage times. These differences varied from 1.05 ± 0.26 log10 cycles (day 1) to 3.30 ± 0.14 log10 cycles (day 5) for nonexcised-skin samples (whole legs) and from 1.54 ± 0.48 log10 cycles (day 1) to 4.28 ± 0.86 log10 cycles (day 5) for excised-skin samples. Significantly larger reductions were observed from the third day of refrigerated storage onward. The TSP concentration was a significant factor in the reduction of L. monocytogenes populations. These results suggest that bacteria are more readily accessible to TSP in excised than in nonexcised chicken skin and that the type of sample used to ascertain the efficacy of antimicrobial surface treatments may influence the findings of this type of study.

Influence of Catfish Skin Mucus on Trisodium Phosphate Inactivation of Attached Salmonella Typhimurium, Edwardsiella tarda, and Listeria monocytogenes

2002 ◽  
Vol 65 (7) ◽  
pp. 1146-1151 ◽  
Author(s):  
JANGHO KIM ◽  
DOUGLAS L. MARSHALL
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Antimicrobial Effect ◽  
Trisodium Phosphate ◽  
Edwardsiella Tarda ◽  
Skin Sample ◽  
Skin Mucus ◽  
Bacterial Counts ◽  
Attached Bacteria ◽  
Antimicrobial Effectiveness

This study examined the antimicrobial effectiveness of trisodium phosphate (TSP) on Edwardsiella tarda, Listeria monocytogenes, and Salmonella Typhimurium attached to catfish skin with and without mucus. Salmonella Typhimurium and E. tarda attached more readily to catfish skin than did L. monocytogenes. At high inoculum levels (107 CFU/ml), TSP treatments (at 2 to 6%) for 10 min reduced bacterial counts of E. tarda by >2.5 to >3.3 log10 CFU per skin sample for firmly attached cells and by 3.5 to 3.6 log10 CFU per skin sample for loosely attached cells. Counts of L. monocytogenes declined by 0.6 to >1.8 log10 CFU per skin sample for firmly attached cells and by 1.2 to 2.2 log10 CFU per skin sample for loosely attached cells. Counts of Salmonella Typhimurium were reduced by 3.6 to >3.8 log10 CFU per skin sample for firmly attached cells and by 3.5 to >3.8 log10 CFU per skin sample for loosely attached cells. Overall, counts of firmly attached bacteria on TSP-treated skins with mucus were higher than counts on skin without mucus. Firmly attached L. monocytogenes was more resistant to TSP than was firmly attached Salmonella Typhimurium or E. tarda. The presence of mucus on skins slightly decreased the antimicrobial effect of TSP. Significant (P < 0.05) reduction in the numbers of all three bacteria can be achieved by treatment with 6% TSP for 10 min.

Efficacy of Trisodium Phosphate Solutions in Reducing Listeria monocytogenes Populations on Chicken Skin during Refrigerated Storage

2001 ◽  
Vol 64 (10) ◽  
pp. 1627-1630 ◽  
Author(s):  
ROSA CAPITA ◽  
CARLOS ALONSO-CALLEJA ◽  
CAMINO GARCÍA-FERNÁNDEZ ◽  
BENITO MORENO
Keyword(s):  
Listeria Monocytogenes ◽  
Trisodium Phosphate ◽  
Refrigerated Storage ◽  
Sterile Water ◽  
Water Control ◽  
Microbial Monitoring ◽  
Chicken Skin ◽  
Phosphate Solutions ◽  
Sampling Times ◽  
Skin Samples

Chicken skin inoculated with 108 CFU/ml of Listeria monocytogenes was dipped for 15 min in sterile water (control) and in 8, 10, or 12% trisodium phosphate (TSP) solutions. Skin samples were stored at 2°C for 5 days, with microbial monitoring on days 0, 1, 3, and 5 after treatment. Compared to the water dip, all TSP treatments significantly (P < 0.05) reduced L. monocytogenes populations on chicken skin. The concentration of the TSP was a significant factor in reducing the populations of the bacteria at days 0, 1, 3, and 5 of refrigerated storage. For all sampling times, the best outcomes were attained with the highest TSP concentration studied (12%). Bacterial reductions in counts during the first day of storage were between 1.52 and 2.70 log10 cycles for 8 and 12% TSP-treated samples, respectively. Significantly greater reductions were observed from the third day of refrigerated storage onward. This occurred largely because populations of L. monocytogenes on control samples increased somewhat, but on TSP-treated samples the pathogen remained practically constant. Differences between L. monocytogenes counts in skin samples immersed in water and those treated with TSP ranged from 2.10 (8% TSP-treated samples) and 3.63 (12% TSP-treated samples) log10 cycles on day 5 of storage. These results indicated that TSP is effective against L. monocytogenes in chicken meat, especially after several days of refrigerated storage.

Maternal treatment with fluoxetine promotes testicular alteration in male rat pups

2016 ◽  
Vol 28 (8) ◽  
pp. 1206 ◽  
Author(s):  
Aline C. Ramos ◽  
Alice H. dos Santos ◽  
Kennia M. Silveira ◽  
Ana Carolina I. Kiss ◽  
Suzana F. P. Mesquita ◽  
...  
Keyword(s):  
Tap Water ◽  
Post Partum ◽  
Gonadosomatic Index ◽  
Male Offspring ◽  
Male Rat ◽  
Control Group ◽  
Maternal Behaviour ◽  
Testis Weight ◽  
Water Control ◽  
Maternal Treatment

Fluoxetine (FLX) is a selective serotonin reuptake inhibitor (SSRI) antidepressant commonly prescribed during pregnancy and lactation. Pre- and post-partum depression, as well as SSRI treatment during these periods, may change maternal care, interfering with offspring development. Moreover, it is known that SSRIs may alter testes structure and function in offspring. The present study investigated the effects of maternal FLX exposure on maternal behaviour and testes function in offspring. Female Wistar rats were treated with 7.5 mg kg–1 FLX or tap water (control group) by gavage from the Day 1 of pregnancy until 21 days after birth (postnatal Day (PND) 21). Maternal behaviour was evaluated and morphofunctional analyses of offspring testes were conducted on PND 21 and 50. There were no significant differences between the FLX-treated and control groups regarding maternal behaviour. Nor did maternal treatment with FLX have any effect on bodyweight gain, anogenital distance, day of preputial separation, testis weight and the gonadosomatic index in male offspring. However, there was a decreased number of Sertoli cells at both PND 21 and 50 in FLX-exposed male offspring. The findings of the present study demonstrate that maternal exposure to FLX can impair testicular function in weanling and pubertal animals.

THE UPTAKE OF TRITIATED LYSINE VASOPRESSIN BY RAT AND PIG PITUITARY NEURAL LOBES IN VITRO

1971 ◽  
Vol 50 (4) ◽  
pp. 669-677 ◽  
Author(s):  
B. A. EDWARDS
Keyword(s):  
Tap Water ◽  
Exchange Chromatography ◽  
Control Group ◽  
Breakdown Product ◽  
Nacl Solution ◽  
Water Control ◽  
Neural Lobe ◽  
Lysine Vasopressin ◽  
And Control

SUMMARY Uptake of tritiated lysine vasopressin ([3H]LVP) was studied in halved neural lobes of rats (which had been given either tap water (control group) or 2% (w/v) NaCl solution as drinking water for 4 days) as well as in slices of pig neural lobe. Uptake of radioactivity into the neural lobes was shown but analysis of the extracts of incubated lobes of both species by ion exchange chromatography showed that very little of it remained in the tissue as hormone. In addition, some radioactivity was associated with trichloroacetic acid-insoluble proteins. After 90 min of incubation, and after correction for the breakdown, the uptake of unchanged [3H]LVP, expressed as a tissue: medium ratio, was 0·14 ± 0·04 and 0·09 ± 0·03 (mean ± s.e.m.) for the saline-treated and control rats respectively, while the tissue: medium ratios for the breakdown product(s) were 6·47 ± 0·45 and 5·50 ± 0·36. The results suggest uptake of [3H]LVP into the cell with almost complete intracellular breakdown of the hormone.

Trisodium phosphate enhanced phage lysis of Listeria monocytogenes growth on fresh-cut produce

LWT ◽  
2017 ◽  
Vol 86 ◽  
pp. 312-317 ◽  
Author(s):  
Adebola O. Oladunjoye ◽  
S. Singh ◽  
Oluwatosin A. Ijabadeniyi
Keyword(s):  
Listeria Monocytogenes ◽  
Trisodium Phosphate ◽  
Fresh Cut

Effect of pH, Acidulant, Time, and Temperature on the Growth and Survival of Listeria monocytogenes

1989 ◽  
Vol 52 (8) ◽  
pp. 571-573 ◽  
Author(s):  
KENT M. SORRELLS ◽  
DAVIN C. ENIGL ◽  
JOHN R. HATFIELD
Keyword(s):  
Antimicrobial Activity ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Citric Acid ◽  
Hydrochloric Acid ◽  
Malic Acid ◽  
Incubation Temperature ◽  
Growth And Survival ◽  
Ph Values

The effect of different acids, pH, incubation time, and incubation temperature on the growth and survival of four strains of Listeria monocytogenes in tryptic soy broth was compared. Hydrochloric acid (HCl), acetic acid (AA), lactic acid (LA), malic acid (MA), and citric acid (CA) were used to acidify tryptic soy broth to pH values 4.4, 4.6, 4.8, 5.0, and 5.2 pH. Incubation times were 1, 3, 7, 14, and 28 d at 10, 25, and 35°C. The inhibition of L. monocytogenes in the presence of high acidity appears to be a function of acid and incubation temperature. Based on equal pH values, the antimicrobial activity is AA > LA > CA ≥ MA > HCl at all incubation times and temperatures. When based on equal molar concentration, the activity appeared to be CA ≥ MA > LA ≥ AA > HCl at 35 and 25°C, and MA > CA > AA ≥ LA > HCl at 10°C. Greatest antimicrobial activity occurred at 35°C. Greatest survival occurred at 10°C and greatest growth occurred at 25°C. Final pH of the medium was as low as 3.8 in HCl at 28 d. All strains grew well at pH values lower than the minimum previously reported (5.5–5.6).

scholarly journals Urban mosquitoes and filamentous green algae: their biomonitoring role in heavy metal pollution in open wastewater channels in Nairobi, Kenya

2021 ◽  
Author(s):  
Geoffrey Kariuki Kinuthia ◽  
Veronica Ngure ◽  
Luna Kamau
Keyword(s):  
Heavy Metals ◽  
Green Algae ◽  
Toxic Elements ◽  
Tap Water ◽  
Aquatic Organisms ◽  
Industrial Area ◽  
Metal Exposure ◽  
Water Control ◽  
Filamentous Green Algae ◽  
Us Epa

Abstract Background Levels of Mercury (Hg), Lead (Pb), Chromium (Cr), Cadmium (Cd), Thallium (Tl), and Nickel (Ni) in samples of wastewater, filamentous green algae (spirogyra) and urban mosquitoes obtained from open wastewater channels in Nairobi industrial area, Kenya, was established. Industrial wastewater may contain hazardous heavy metals upon exposure. Aquatic organisms in wastewater may accumulate the toxic elements with time. Therefore, human population living in informal settlements in Nairobi industrial area risk exposure to such toxic elements. Biomonitoring using aquatic organisms can be key in metal exposure assessment. Results Pb, Cr, & Ni levels ranged from 3.08 to 15.31 µg/L while Tl, Hg, & Cd levels ranged from 0.05 to 0.12 µg/L in wastewater. Pb, Cr, Ni, & Cd levels were above WHO, Kenya & US EPA limits for wastewater but Hg was not. Metals in tap water (control) which ranged from 0.01 to 0.2 µg/L was below WHO, US EPA, & Kenya standard limits. Pb, Cr, Tl, & Ni levels in assorted field mosquitoes were 1.3 to 2.4 times higher than in assorted laboratory-reared mosquitoes. Hg & Cd concentrations in laboratory-reared mosquitoes (0.26 mg/L & 1.8 mg/L respectively) was higher than in field mosquitoes (0.048 mg/L & 0.12 mg/L respectively). Pb, Cr, Ni, & Cd levels in green filamentous algae were 110.62, 29.75, 14.45, & 0.44 mg/L respectively and above WHO limits for vegetable plants. Hg level in algae samples (0.057 mg/L) was below WHO standard limits but above Kenya & US EPA limits in vegetables. Correlations for Pb & Hg (r = 0.957; P < 0.05); Cd & Cr (r = 0.985; P < 0.05) in algae samples were noted. The metal concentrations in the samples were in the order, wastewater < mosquitoes < filamentous green algae. Conclusion Samples of wastewater, mosquitoes and filamentous green algae from open wastewater channels and immediate vicinity, in Nairobi industrial area (Kenya) contained Hg, Pb, Cr, Cd, Tl, and Ni. Urban mosquitoes and filamentous green algae can play a role of metal biomonitoring in wastewater. The possibility of urban mosquitoes transferring the heavy metals to their hosts when sucking blood should be investigated.

scholarly journals Vermiculture as a biotechnological method of recultivation of disturbed lands in the post-mining system

2021 ◽  
Vol 315 ◽  
pp. 02008
Author(s):  
Andrei Babenko ◽  
Dmitrij Bezrukov ◽  
Saulet Karimova ◽  
Akmaral Mametova ◽  
Ludmila Zakonnova
Keyword(s):  
Leaf Litter ◽  
Calcium Ions ◽  
Tap Water ◽  
Mining System ◽  
Mining Areas ◽  
Ph Values ◽  
Disturbed Areas ◽  
Agrochemical Properties ◽  
Plant Waste ◽  
Poplar Leaf

The article shows the prospects of using the technology of vermicomposting for the biological re-cultivation of disturbed mining areas. The valuable properties of vermicompost as a component of soil-substituting mixtures for use in the re-cultivation of disturbed areas are considered. The expediency of using plant waste for the preparation of soil-substituting substrates is shown. The presence of special agrochemical properties of vermicompost obtained during the processing of plant waste (leaf litter) by the Eisenia fetida Savigny culture is experimentally established. The pH values and the content of calcium ions in the vermicompost based on poplar leaf litter were significantly higher than in the vermicompost based on horse manure – 7.61 vs. 6.98 and 112.3 vs. 83 mg-eq. / kg, respectively. Germination of wheat seeds on extracts from vermicompost obtained from poplar litter led to a statistically significant increase in the mass of the formed roots compared to seeds germinated on tap water.

Sign in / Sign up

Export Citation Format

Share Document