Maternal treatment with fluoxetine promotes testicular alteration in male rat pups

2016 ◽  
Vol 28 (8) ◽  
pp. 1206 ◽  
Author(s):  
Aline C. Ramos ◽  
Alice H. dos Santos ◽  
Kennia M. Silveira ◽  
Ana Carolina I. Kiss ◽  
Suzana F. P. Mesquita ◽  
...  
Keyword(s):  
Tap Water ◽  
Post Partum ◽  
Gonadosomatic Index ◽  
Male Offspring ◽  
Male Rat ◽  
Control Group ◽  
Maternal Behaviour ◽  
Testis Weight ◽  
Water Control ◽  
Maternal Treatment

Fluoxetine (FLX) is a selective serotonin reuptake inhibitor (SSRI) antidepressant commonly prescribed during pregnancy and lactation. Pre- and post-partum depression, as well as SSRI treatment during these periods, may change maternal care, interfering with offspring development. Moreover, it is known that SSRIs may alter testes structure and function in offspring. The present study investigated the effects of maternal FLX exposure on maternal behaviour and testes function in offspring. Female Wistar rats were treated with 7.5 mg kg–1 FLX or tap water (control group) by gavage from the Day 1 of pregnancy until 21 days after birth (postnatal Day (PND) 21). Maternal behaviour was evaluated and morphofunctional analyses of offspring testes were conducted on PND 21 and 50. There were no significant differences between the FLX-treated and control groups regarding maternal behaviour. Nor did maternal treatment with FLX have any effect on bodyweight gain, anogenital distance, day of preputial separation, testis weight and the gonadosomatic index in male offspring. However, there was a decreased number of Sertoli cells at both PND 21 and 50 in FLX-exposed male offspring. The findings of the present study demonstrate that maternal exposure to FLX can impair testicular function in weanling and pubertal animals.

Maternal protein restriction in pregnancy and/or lactation affects seminiferous tubule organization in male rat offspring

2012 ◽  
Vol 3 (5) ◽  
pp. 321-326 ◽  
Author(s):  
G. L. Rodríguez-González ◽  
R. M. Vigueras-Villaseñor ◽  
S. Millán ◽  
N. Moran ◽  
R. Trejo ◽  
...  
Keyword(s):  
Body Weight ◽  
Germ Cell ◽  
Control Diet ◽  
Protein Restriction ◽  
Male Offspring ◽  
Male Rat ◽  
Testis Weight ◽  
Germ Cell Differentiation ◽  
Maternal Protein Restriction ◽  
Cell Proliferation And Differentiation

Maternal protein restriction (MPR) during pregnancy impaired the reproduction of male offspring. We investigated, during the first wave of spermatogenesis, whether MPR exerts deleterious effects on germ cell proliferation and differentiation, as well as androgen receptor (AR) protein expression, which was used as a marker for Sertoli cell (SC) maturation. At the beginning of pregnancy (day 0), dams were fed a control diet (C: 20% casein) or a restricted isocaloric diet (R: 10% casein). After birth, four groups were established: CC, RR, CR and RC (first letter diet during pregnancy and second during lactation). Male offspring were studied at postnatal days 14, 21 and 36. At birth, pup body weight was unchanged. Body weight and testis weight were reduced in RR and CR groups at all ages evaluated. MPR delayed the germinal epithelium development at all ages evaluated. On performing Western blot and immunohistochemistry, AR expression was found to be lower in the three restricted groups. The results suggest that MPR during pregnancy and/or lactation delays SC maturation and germ cell differentiation, and affects intratubular organization. These changes might be responsible for the lower fertility rate at older ages.

THE UPTAKE OF TRITIATED LYSINE VASOPRESSIN BY RAT AND PIG PITUITARY NEURAL LOBES IN VITRO

1971 ◽  
Vol 50 (4) ◽  
pp. 669-677 ◽  
Author(s):  
B. A. EDWARDS
Keyword(s):  
Tap Water ◽  
Exchange Chromatography ◽  
Control Group ◽  
Breakdown Product ◽  
Nacl Solution ◽  
Water Control ◽  
Neural Lobe ◽  
Lysine Vasopressin ◽  
And Control

SUMMARY Uptake of tritiated lysine vasopressin ([3H]LVP) was studied in halved neural lobes of rats (which had been given either tap water (control group) or 2% (w/v) NaCl solution as drinking water for 4 days) as well as in slices of pig neural lobe. Uptake of radioactivity into the neural lobes was shown but analysis of the extracts of incubated lobes of both species by ion exchange chromatography showed that very little of it remained in the tissue as hormone. In addition, some radioactivity was associated with trichloroacetic acid-insoluble proteins. After 90 min of incubation, and after correction for the breakdown, the uptake of unchanged [3H]LVP, expressed as a tissue: medium ratio, was 0·14 ± 0·04 and 0·09 ± 0·03 (mean ± s.e.m.) for the saline-treated and control rats respectively, while the tissue: medium ratios for the breakdown product(s) were 6·47 ± 0·45 and 5·50 ± 0·36. The results suggest uptake of [3H]LVP into the cell with almost complete intracellular breakdown of the hormone.

Early Post-Partum Contact and Later Attitudes

1980 ◽  
Vol 3 (3) ◽  
pp. 273-286 ◽  
Author(s):  
Peter de Chateau
Keyword(s):  
Post Partum ◽  
Postnatal Period ◽  
Child Rearing ◽  
Control Group ◽  
Maternal Behaviour ◽  
Relative Importance ◽  
Long Term Effects ◽  
Infant Behaviour

In a randomized, prospective study the long-term effects of early post-partum skin-toskin and suckling contact was studied. In follow-up studies 36 hours, 3 and 12 months after delivery maternal behaviour, infant behaviour, the duration of breast feeding and certain attitudes towards child rearing procedures were shown to develop differently in a group of mothers and infants with early post-natal contacts as compared to a control group. Three years after delivery parents with early contact appreciated their children's language development to be faster; the number of siblings born in these families was greater than in controls. In the discussion, the relative importance of the immediate postnatal period is emphasized, a more family oriented development seems to occur in the presence of early post-delivery interaction.

scholarly journals Effects of hydrogen peroxide on diazepam and xylazine sedation in chicks

2012 ◽  
Vol 5 (4) ◽  
pp. 179-183 ◽  
Author(s):  
Yaareb J. Mousa ◽  
Fouad K. Mohammad
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Recovery Time ◽  
Tap Water ◽  
Statistical Significance ◽  
Control Group ◽  
Dependent Manner ◽  
Water Control ◽  
Whole Brain ◽  
Total Recovery

ABSTRACT Oxidative stress may cause various neuronal dysfunctions and modulate responses to many centrally acting drugs. This study examines the effects of oxidative stress produced by hydrogen peroxide (H2O2) on sedation induced by diazepam or xylazine as assessed in 7-14 day-old chicks. Day-old chicks were provided with either plane tap water (control group) or H2O2 in tap water as 0.5% v/v drinking solution for two weeks in order to produce oxidative stress. Spectrophotometric methods were used to determine glutathione and malondialdehyde concentrations in plasma and whole brain. Drug-induced sedation in the chicks was assessed by monitoring the occurrence of signs of sedation manifested as drooping of the head, closed eyelids, reduced motility or immotility, decreased distress calls, and recumbency. The latency to onset of sedation and its duration were also recorded. H2O2 treatment for two weeks significantly decreased glutathione and increased malondialdehyde concentrations in plasma and whole brain of the chicks on days 7, 10 and 14 as compared with respective age-matched control groups. H2O2 decreased the median effective doses of diazepam and xylazine for the induction of sedation in chicks by 46% and 63%, respectively. Injection of diazepam at 2.5, 5 and 10 mg/kg, i.m. or xylazine at 2, 4 and 8 mg/kg, i.m. induced sedation in both control and H2O2-treated chicks in a dose dependent manner, manifested by the above given signs of sedation. H2O2 significantly decreased the latency to onset of sedation in chicks treated with diazepam at 5 and 10 mg/kg, increased the duration of sedation and prolonged the total recovery time in comparison with respective non-stressed control chicks. A similar trend occurred with xylazine in the H2O2-treated chicks, though the differences from control counterparts did not attain the statistical significance, except for the recovery time of the lowest dose of the drug. The data suggest that H2O2-induced oxidative stress sensitizes the chicks to the depressant action of the sedatives diazepam and xylazine. Further studies are needed to examine the potential role of oxidative stress in modulating the actions of therapeutic agents on the brain.

Cardiac sympathetic overactivity and decreased baroreflex sensitivity in l-NAME hypertensive rats

2001 ◽  
Vol 280 (2) ◽  
pp. H844-H850 ◽  
Author(s):  
Hugo C. D. Souza ◽  
Gustavo Ballejo ◽  
Maria Cristina O. Salgado ◽  
Valdo J. Dias Da Silva ◽  
Helio C. Salgado
Keyword(s):  
Heart Rate ◽  
Methyl Ester ◽  
Baroreflex Sensitivity ◽  
Tap Water ◽  
Low Frequency ◽  
Control Group ◽  
Fast Fourier Transformation ◽  
Hypertensive Rats ◽  
Water Control ◽  
Sympathetic Overactivity

The present study evaluated the possible changes in the autonomic control of heart rate in the hypertensive model induced by the inhibition of nitric oxide synthase. Rats were treated with N G-nitro-l-arginine methyl ester (l-NAME group) in the drinking water during 7 days, whereas control groups were treated with tap water (control group) or with the N G-nitro-d-arginine methyl ester (d-NAME group), an inactive isomer of thel-NAME molecule. The l-NAME group developed hypertension and tachycardia. The sequential blockade of the autonomic influences with propranolol and methylatropine indicated that the intrinsic heart rate did not differ among groups and revealed a sympathetic overactivity in the control of heart rate in thel-NAME group. The spectral density power of heart rate, calculated using fast-Fourier transformation, indicated a reduced variability in the low-frequency band (0.20–0.60 Hz) for thel-NAME group. The baroreflex sensitivity was also attenuated in these animals when compared with the normotensive control or d-NAME group. Overall, these data indicate cardiac sympathetic overactivity associated with a decreased baroreflex sensitivity in l-NAME hypertensive rats.

Maternal exposure to low doses of bisphenol A affects learning and memory in male rat offspring with abnormal N-methyl-d-aspartate receptors in the hippocampus

2021 ◽  
pp. 074823372098462
Author(s):  
Chong Wang ◽  
Yao Shu ◽  
Li Xu ◽  
Qiling Liu ◽  
Bei Zhang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Learning And Memory ◽  
Maternal Exposure ◽  
Male Offspring ◽  
Male Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Expression Levels ◽  
Rat Offspring ◽  
Sprague Dawley Rats

Bisphenol A (BPA), a component of polycarbonate and epoxy resins, has been reported to induce learning and memory deficits. However, the mechanisms have not been fully elucidated. Growing evidence has suggested that N-methyl-d-aspartate receptors (NMDARs) are involved in cognitive impairments. In this study, BPA was administered to female Sprague–Dawley rats (six per dose group) at concentrations of 0 (control), 4, 40, and 400 μg/kg·body weight/day from gestation day 1 through lactation day 21. Spatial learning was evaluated using the Morris water maze on postnatal day 22. Expression levels of NMDARs were determined using real-time polymerase chain reaction and Western blot. The results showed that male offspring exposed to BPA exhibited increased latency in reaching the platform and reduced time in the target quadrant, and the number of crossing the platform was less, as compared with the control group. The mRNA and protein expression levels of NMDARs in the hippocampus were significantly downregulated when compared with the control group of male offspring. The data showed that maternal exposure to BPA at low dosage can cause cognitive deficits in male rat offspring, probably due to a decrease in NMDARs in the hippocampus.

scholarly journals The protective role of alcoholic extract of (Anethum graveolens) seeds on renal function in alloxan induced diabetic rabbits

2015 ◽  
Vol 39 (2) ◽  
pp. 1-6
Author(s):  
Luma W. Khalil
Keyword(s):  
Diabetes Mellitus ◽  
Renal Function ◽  
Adult Female ◽  
Tap Water ◽  
Diabetic Group ◽  
Control Group ◽  
Alcoholic Extract ◽  
Serum Urea ◽  
Anethum Graveolens ◽  
Water Control

     This study was designed to investigate the effect of dill (Anethum graveolens) seeds in mitigating the complications of induced diabetes mellitus such as nephropathy of adult female rabbits. Thirty adult female rabbits were randomly assigned to 5 equal groups and treated daily for 45 days as follows: Control group (C) drenched tap water, control group intubated orally with 700 mg/kg B.W ethanolic extract of dill (T1), diabetic group drenched tap water (T2), diabetic group intubated orally700 mg/kg B.W of dill (T3) and diabetic group treated with insulin injection 3 I.U S/C (T4). Blood samples were collected by heart puncture at 0 and 45 days of experiment to assess renal efficiency: Serum creatinine, serum urea, and serum glutathione (GSH). The results indicated that diabetes mellitus without treatment group (T2) caused renal damage manifested by significant (P<0.05) increase in creatinine, urea and significant (P<0.05) decrease in serum glutathione (GSH) concentration. Oral intubation of alcoholic extract of dill seeds in diabetic treated group (T3) improved renal function through significant (P<0.05) decrease in serum urea, creatinine, and significant (P<0.05) increase in serum glutathione concentration (GSH). It was concluded that alcoholic extract of dill seeds was effective in reducing the complications of diabetes mellitus such as nephropathy.

Generational reproductive outcomes in Wistar rats maternally exposed to Ricinus communis oil at different stages of gestation

2015 ◽  
Vol 6 (5) ◽  
pp. 443-453
Author(s):  
S. A. Salami ◽  
Y. Raji
Keyword(s):  
Birth Weight ◽  
Ricinus Communis ◽  
Sperm Count ◽  
Maternal Exposure ◽  
Control Group ◽  
Testis Weight ◽  
Critical Periods ◽  
Morphometric Data ◽  
Water Control ◽  
Pregnant Rats

Fetal programming hypothesis presupposes that stimulus or insult acting during critical periods of uterine growth and development may permanently alter tissue structure and function. Ricinus communis oil (RCO) has been reported to possess/used as laxative, labor-inducing and estrogenic properties. Generational reproductive effects of maternal exposure to RCO was investigated in rats. A total of 25 pregnant rats randomly assigned to five equal groups were treated with distilled water (control, group 1), RCO (950 mg/kg p.o.) during gestation days (GD) 1–7, 7–14, 14–21 and 1–21, respectively. Birth weight, morphometric data, anogenital distance (AGD), pubertal age, sperm parameters, hormonal profile, organ weight and histopathology were determined in the first (F1) and second (F2) filial generations. Results showed a significant decrease (P<0.05) in birth weight/morphometric data in male pups from the GD 1–7 and 7–14 groups. AGD decreased significantly in RCO-treated F1 males. Pubertal age of F1 females decreased significantly (P<0.05) compared with controls. At postnatal day 90, F1 males from the RCO-treated group showed significant decrease in testis weight, body weight, sperm count, motility and normal morphology. Testosterone levels were significantly decreased in RCO-treated F1 males, which also showed testicular interstitial edema and epididymal hypospermia. Only pubertal indexes were altered in F2 rats. Maternal exposure to RCO at early gestation periods impaired androgen-mediated reproductive end points in the first generation of rats. RCO exhibits endocrine disrupting capabilities.

scholarly journals Antifertility effect of betel nut (Areca catechu L) in male rat

MEDISAINS ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 52
Author(s):  
Ave Olivia Rahman ◽  
Anati Purwakanthi ◽  
Hasna Dewi
Keyword(s):  
Negative Control ◽  
Male Rats ◽  
Male Rat ◽  
Seminiferous Tubules ◽  
Control Group ◽  
High Dose ◽  
Testis Weight ◽  
Betel Nut ◽  
Areca Catechu ◽  
Group 2

Background: Betel nut (Areca catechu L) are traditional beverages in Jambi. Betel nut high dose has a cytotoxic effect on testis and other organs—limited data about the effect of betel nut as male antifertility.Purposes:  This study aimed to determine the effect of infertility on betel nut use at a dose of 50 mg/kg body weight (BW) in male rats.   Methods: Ten male Sprague Dawney rats that had passed the fertility test, aged 2-3 months and weighing 150-200 grams ware used in this study. The rat was grouped into two groups randomly. Group 1, as the negative control, were received aqua dest, and group 2 was treated by betel nut with dose 50 mg/kg BW for 35 days. When terminated, testis weight weighed using micro scales. Blood collected for measuring testosterone levels. Histopathology assessment of testis used Hematoxylin Eosin Staining and sperm counting from cauda epididymis.Results: The weight of testis in the group received betel nut was lower than the control group. The histopathology of testis showed shrunk, reduced the diameter of seminiferous tubules, and like cytolytic lesions in the germinal layer—the total sperm number and progressive sperms also lower than the control group (p<0.05). There was no abnormality in Leydig cell and interstitium tissue.  Male rats after treatment could not impregnate the female rats.Conclusion: Betel nut at dose 50 mg/kg BW have antifertility activity in male rats.

Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Ni Made Ridla Parwata
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Male Rat ◽  
Control Group ◽  
Adult Male Rat ◽  
The Subject ◽  
Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

Sign in / Sign up

Export Citation Format

Share Document