Occurrence of Zoonotic Clostridia and Yersinia in Healthy Cattle

2013 ◽  
Vol 76 (10) ◽  
pp. 1697-1703 ◽  
Author(s):  
A. SCHMID ◽  
U. MESSELHÄUSSER ◽  
S. HÖRMANSDORFER ◽  
C. SAUTER-LOUIS ◽  
R. MANSFELD
Keyword(s):  
Beef Cattle ◽  
Clostridium Botulinum ◽  
Dairy Farms ◽  
Cattle Farm ◽  
Toxin B ◽  
Fecal Samples ◽  
Toxin Genes ◽  
Healthy Cattle ◽  
Study Population ◽  
Cattle Farms

Zoonotic pathogens are a frequent cause of disease worldwide. This study was designed to determine the occurrence of Clostridium difficile, Clostridium botulinum, and Yersinia enterocolitica in cattle in southern Bavaria, Germany. The study population included 49 farms; 34 were dairy farms (30 also fattening beef cattle) and 15 were solely beef cattle farms. Fecal and dust samples were collected from summer 2011 to summer 2012 and analyzed using a combination of enrichment procedures and real-time PCR. For the detection of C. difficile, samples were screened for the presence of the tpi gene and toxin genes tcdA, tcdB, and cdtA. Samples also were screened for genes for C. botulinum toxins A through F and for the ail gene of Y. enterocolitica. Of 506 samples, C. difficile genes were found in 29 samples (5.7%): 25 samples from dairy farms and 4 samples from beef cattle farms. Toxin genes were identified in 17 samples, with toxigenic profiles of A+B+CDT−, A+B−CDT+, and A+B+CDT+. C. botulinum toxin genes were not detected in fecal samples from cattle, but the gene for toxin B was detected in 1 (0.8%) of 125 dust samples. Y. enterocolitica genes were found in 6 (1.6%) of 382 fecal samples from three dairy farms and one beef cattle farm. This study revealed that C. difficile and Y. enterocolitica are rare on cattle farms in Bavaria, Germany. In contrast to results of previous studies, C. botulinum was not detected in fecal samples but was found very rarely in dust samples from the cattle environment.

scholarly journals Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

2013 ◽  
Vol 79 (9) ◽  
pp. 3027-3032 ◽  
Author(s):  
A. Schmid ◽  
S. Hörmansdorfer ◽  
U. Messelhäusser ◽  
A. Käsbohrer ◽  
C. Sauter-Louis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Dairy Cows ◽  
Fecal Samples ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Study Population ◽  
Cattle Farms ◽  
Group 1

ABSTRACTExtended-spectrum β-lactamase (ESBL)-producingEscherichia colistrains are believed to be widely distributed among humans and animals; however, to date, there are only few studies that support this assumption on a regional or countrywide scale. Therefore, a study was designed to assess the prevalence of ESBL-producingE. coliin dairy cows and beef cattle in the southern part of Bavaria, Germany. The study population included 30 mixed dairy and beef cattle farms and 15 beef cattle farms. Fecal samples, boot swabs, and dust samples were analyzed for ESBL-producingE. coliusing selective media. PCR was performed to screen for CTX-M andampCresistance genes. A total of 598 samples yielded 196 (32.8%) that contained ESBL-producingE. coli, originating from 39 (86.7%) of 45 farms. Samples obtained from mixed farms were significantly more likely to be ESBL-producingE. colipositive than samples from beef cattle farms (fecal samples,P< 0.001; boot swabs,P= 0.014; and dust samples,P= 0.041). A total of 183 isolates (93.4%) of 196 ESBL-producingE. coli-positive strains harbored CTX-M genes, CTX-M group 1 being the most frequently found group. Forty-six additional isolates containedampCgenes, and 5 of the 46 isolates expressed ablaCMY-2gene. The study shows that ESBL-producingE. colistrains are commonly found on Bavarian dairy and beef cattle farms. Moreover, to our knowledge, this is the first report of the occurrence ofblaCMY-2in cattle in Germany.

scholarly journals Sistema de información para la gestión de inventario y actividades en un hato ganadero

2020 ◽  
Vol 41 (50) ◽  
pp. 215-230
Author(s):  
Roberto C. AHUMADA ◽  
◽  
Luis CERVANTES ◽  
Raul J. MARTELO ◽  
◽  
...  
Keyword(s):  
Information System ◽  
Cattle Farm ◽  
Study Population ◽  
Cattle Farms

In this research, an information system was developed for the management of inventory and activities in a cattle farm in Bolívar, Colombia. The development was carried out under the SCRUM and RUP methodologies. The study population was made up of the owners of cattle farms, and the sample was 40 farmers. Regarding the results, the system will allow the farmer to optimally manage the animals in his charge.

Prevalence of Neospora caninum Infection in Dogs from Beef-Cattle Farms, Dairy Farms, and from Urban Areas of Argentina

2001 ◽  
Vol 87 (4) ◽  
pp. 906 ◽  
Author(s):  
W. Basso ◽  
L. Venturini ◽  
M. C. Venturini ◽  
P. Moore ◽  
M. Rambeau ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Urban Areas ◽  
Neospora Caninum ◽  
Dairy Farms ◽  
Caninum Infection ◽  
Cattle Farms

Prevalence of Neospora caninum Infection in Dogs From Beef-Cattle Farms, Dairy Farms, and From Urban Areas of Argentina

2001 ◽  
Vol 87 (4) ◽  
pp. 906-907 ◽  
Author(s):  
W. Basso ◽  
L. Venturini ◽  
M. C. Venturini ◽  
P. Moore ◽  
M. Rambeau ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Urban Areas ◽  
Neospora Caninum ◽  
Dairy Farms ◽  
Caninum Infection ◽  
Cattle Farms

scholarly journals Matching sheep and beef policies to pasture supply on Northland hill country

1995 ◽  
pp. 207-212
Author(s):  
C.R. Page ◽  
R.D. Thomson ◽  
R.W. Webby
Keyword(s):  
Weight Gain ◽  
Beef Cattle ◽  
Cattle Farm ◽  
Pasture Production ◽  
Hill Country ◽  
The Past ◽  
Group Members ◽  
Full Benefit ◽  
Cattle Farms ◽  
Made In

Farm monitoring on sheep and beef cattle farms in Northland has been in operation for the past 8 years. The emphasis has been on monitoring the performance of sheep and beef cattle farms to provide the data to encourage group members to make decisions based on objective measurements. Monitor groups were initially supported by extension and science personnel with more recent groups being operated on a commercial basis. Key biological indices such as animal numbers, weights, weight gain, meat and wool production and pasture production are monitored. Group members are encouraged to use information from the monitor farm to identify opportunities on their own farms for improvement in production and income. Significant gains have been made in production but it can take 3 to 5 years for the full benefit to be realised. Pasture production information has been v,ital to identify changes in feed supply from year to year during the monitoring programme. Farm monitoring in the future is likely to be the basis on which farmers will be able to. meet year-round supply of product based on specifications of weight, date and carcass attributes. Keywords: beef and cattle, farm monitoring, objective measurement, pasture production, sheep

scholarly journals Pencemaran Bakteri dalam Air Sumur di Sekitar Peternakan Sapi Potong di Yogyakarta

2016 ◽  
Vol 2 (2) ◽  
pp. 43-48
Author(s):  
Widodo Suwito ◽  
. Supriadi ◽  
Erna Winarti ◽  
Nyoman Ayu Anggreni Tisnawati
Keyword(s):  
Beef Cattle ◽  
Water Samples ◽  
Most Probable Number ◽  
Well Water ◽  
Isolation And Identification ◽  
Cattle Farm ◽  
Probable Number ◽  
E Coli ◽  
Household Water ◽  
Cattle Farms

Air sumur merupakan salah satu sumber air untuk keperluan rumah tangga. Tujuan dari penelitian ini adalah untuk mengetahui kualitas mikrobiologi air sumur di sekitar kandang kelompok sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 12 contoh air sumur di sekitar kandang kelompok sapi potong dari Kabupaten Sleman, Kulon Progo, dan Bantul. Contoh air sumur diperiksa terhadap Coliform dan E. coli dengan metode most probable number (MPN), sedangkan Salmonella sp. dengan isolasi dan identifikasi dengan metode Andrews & Hammack. Sebanyak 91,6% dari 12 contoh air sumur, jumlah Coliform dan E. coli melebihi ambang batas baku mutu air rumah tangga. Salmonella sp. berhasil diisolasi dari air sumur di sekitar kandang sapi potong Kabupaten Kulon Progo. Dari hasil penelitian dapat disimpulkan bahwa air sumur di sekitar kandang di Yogyakarta hampir seluruhnya tercemar Coliform dan E. Coli.Kata kunci: pencemaran, air sumur, peternakan, sapi potong. (Contamination of Bacteria in Well Water Around Beef Cattle Farm in Yogyakarta)Well water is one of the sources of water to use in housewifery. The aim of this study was to determine microbiological contamination of well water around beef cattle farms in Yogyakarta. A total of 12 well water samples were collected from around beef cattle farms in Sleman, Kulon Progo, and Bantul district. These samples were analyzed for Coliform and E. coli by using most probable number (MPN), where as Salmonella sp. with isolation and identification by Andrews & Hammack methods. A total 91.6% of 12 well water samples have Coliform and E. coli that exceeds the threshold household water quality standards. Salmonella sp. was isolated from well water around beef cattle farm in Kulon Progo district. In conclusion, the well water samples around beef cattle farms in Yogyakarta contaminated Coliform and E. coli.Keywords: contamination, well water, livestock, beef cattle.

scholarly journals Effects of farm monitoring on Northland's small beef cattle farms

1995 ◽  
pp. 213-215
Author(s):  
G.R. Ussher ◽  
D.A. Low ◽  
S. Brown
Keyword(s):  
Beef Cattle ◽  
Business Planning ◽  
Cattle Farm ◽  
Far North ◽  
Fertiliser Use ◽  
Evaluation And Monitoring ◽  
Farm Programme ◽  
Stock Policy ◽  
Farm Business ◽  
Cattle Farms

Results from the MRDC-ANZ monitor farm programme in the Far North (Omatua Farm Ltd) have involved the adoption or continued use of onfarm technologies such as crossbreeding, yearling mating, regrassing, supplementary feeding, subdivision, fertiliser use, stock policy evaluation and monitoring animal growth rates. These results have substantially improved farm productivity and profitability. Farm business planning has been a significant part of the monitor farm process, leading to a 12% increase in yearly beef produced off the monitor farm. Small farms like Omatua Farm Ltd make up 63% of the beef cattle and sheep farms in the Far North District Council. The potential of the farm monitor-business planning approach on these farms is to improve gross annual income by $20 000. Without improvements in productivity and profitability an increasing amount of beef cattle farm land in the area is likely to end up in an alternative land use. Keywords: business planning, farm management technologies, monitor farm, small beef cattle farms

scholarly journals Development of a Combined Selection and Enrichment PCR Procedure for Clostridium botulinum Types B, E, and F and Its Use To Determine Prevalence in Fecal Samples from Slaughtered Pigs

2001 ◽  
Vol 67 (10) ◽  
pp. 4781-4788 ◽  
Author(s):  
Maria Dahlenborg ◽  
Elisabeth Borch ◽  
Peter Rådström
Keyword(s):  
Sample Preparation ◽  
Fecal Sample ◽  
Clostridium Botulinum ◽  
Geographical Location ◽  
Type B ◽  
Fecal Samples ◽  
Pcr Assays ◽  
Combined Selection ◽  
Spore Load ◽  
Slaughtered Pigs

ABSTRACT A specific and sensitive combined selection and enrichment PCR procedure was developed for the detection of Clostridium botulinum types B, E, and F in fecal samples from slaughtered pigs. Two enrichment PCR assays, using the DNA polymeraserTth, were constructed. One assay was specific for the type B neurotoxin gene, and the other assay was specific for the type E and F neurotoxin genes. Based on examination of 29 strains of C. botulinum, 16 strains of other Clostridium spp., and 48 non-Clostridium strains, it was concluded that the two PCR assays detect C. botulinum types B, E, and F specifically. Sample preparation prior to the PCR was based on heat treatment of feces homogenate at 70°C for 10 min, enrichment in tryptone-peptone-glucose-yeast extract broth at 30°C for 18 h, and DNA extraction. The detection limits after sample preparation were established as being 10 spores per g of fecal sample for nonproteolytic type B, and 3.0 × 103 spores per g of fecal sample for type E and nonproteolytic type F with a detection probability of 95%. Seventy-eight pig fecal samples collected from slaughter houses were analyzed according to the combined selection and enrichment PCR procedure, and 62% were found to be PCR positive with respect to the type B neurotoxin gene. No samples were positive regarding the type E and F neurotoxin genes, indicating a prevalence of less than 1.3%. Thirty-four (71%) of the positive fecal samples had a spore load of less than 4 spores per g. Statistical analysis showed that both rearing conditions (outdoors and indoors) and seasonal variation (summer and winter) had significant effects on the prevalence of C. botulinum type B, whereas the effects of geographical location (southern and central Sweden) were less significant.

scholarly journals Shiga Toxins, and the Genes Encoding Them, in Fecal Samples from Native Idaho Ungulates

2008 ◽  
Vol 75 (3) ◽  
pp. 862-865 ◽  
Author(s):  
Jeremy J. Gilbreath ◽  
Malcolm S. Shields ◽  
Rebekah L. Smith ◽  
Larry D. Farrell ◽  
Peter P. Sheridan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Wild Ungulates ◽  
Fecal Samples ◽  
Shiga Toxins ◽  
Toxin Genes ◽  
Genes Encoding ◽  
Shiga Toxin Genes

ABSTRACT Cattle are a known reservoir of Shiga toxin-producing Escherichia coli. The prevalence and stability of Shiga toxin and/or Shiga toxin genes among native wild ungulates in Idaho were investigated. The frequency of both Shiga genes and toxin was similar to that reported for Idaho cattle (∼19%).

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