Effects of Chitosan and Natamycin on Vacuum-Packaged Phyllo: A Pastry Product

2018 ◽  
Vol 81 (12) ◽  
pp. 1982-1987 ◽  
Author(s):  
MARIA I. TSIRAKI ◽  
TAHRA EL-OBEID ◽  
HANY M. YEHIA ◽  
LAYAL KARAM ◽  
IOANNIS N. SAVVAIDIS
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Vacuum Packaging ◽  
Life Extension ◽  
Sensory Acceptability ◽  
Psychrotrophic Bacteria ◽  
Sensory Changes ◽  
Yeasts And Molds ◽  
Microbiological Data

ABSTRACT The effects of vacuum packaging, chitosan (1.5%, w/v), and natamycin (10 mg/L, w/v) on phyllo pastry quality were studied by monitoring microbiological, chemical, and sensory changes. Five lots were prepared with or without vacuum packaging, chitosan, and natamycin: A, air packaged (control); V, vacuum packaged; VC, vacuum packaged with chitosan; VN, vacuum packaged with natamycin; and VCN, vacuum packaged with both chitosan and natamycin. Based on the sensory acceptability data, a shelf life of 6 (A), 12 (V), 14 (VN), 16 (VC), and 17 (VCN) days was obtained at 4°C. VCN treatment resulted in a shelf life extension of 11 days compared with the shelf life of the control. Microbiological data revealed that the combination of chitosan and natamycin resulted in significant reductions of microbial species (mesophilic total viable counts, yeasts and molds, psychrotrophic bacteria, lactic acid bacteria, Enterobacteriaceae, and enterococci) of 1 to 3 log CFU/g on the final day (day 18) of storage. Results suggest that the combination of chitosan and natamycin, which is an effective antifungal agent, can delay the spoilage of phyllo pastry while maintaining acceptable sensorial characteristics and the original freshness and appearance of the product.

Sodium Lactate Addition on the Quality and Shelf Life of Refrigerated Sliced Poultry Sausage Packaged in Air or Nitrogen Atmosphere

2004 ◽  
Vol 67 (3) ◽  
pp. 601-606 ◽  
Author(s):  
RENATA CEGIELSKA-RADZIEJEWSKA ◽  
JAN PIKUL
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Sodium Lactate ◽  
Life Extension ◽  
Poultry Meat ◽  
Color Measurement ◽  
Refrigerated Storage ◽  
Psychrotrophic Bacteria

The aim of this study was to determine the effect of sodium lactate addition on shelf-life extension of sliced poultry sausage packaged both in air and nitrogen atmospheres and stored in refrigerated conditions. Basic chemical composition, pH, and malonaldehyde content were assayed and color measurement using the reflection method was carried out. Microbiological examination consisted of determination of total number of aerobic psychrotrophic bacteria and number of lactic acid bacteria. Sensory evaluation of products was performed. Microbiological and sensory quality of sliced poultry meat sausage was dependent on the addition during production of sodium lactate and the composition of gases (air or nitrogen) used in packaging. Slices of poultry sausage with 1% as well as 2% of sodium lactate maintained their initial quality of evaluated sensory attributes longer, irrespective of the applied gases. Sodium lactate inhibited growth of aerobic psychrotrophic bacteria and lactic acid bacteria during refrigerated storage. Sodium lactate also inhibited the formation of malonaldehyde in sliced poultry sausage during refrigerated storage. The effectiveness of this process depended on the concentration of sodium lactate addition. It was concluded that 1% as well as 2% addition of sodium lactate could extend the shelf life of sliced poultry sausage packaged in air atmosphere and stored at 5 to 7°C by 3 or 4 times, respectively. Sliced poultry sausage treated with 2% sodium lactate packed in nitrogen had the longest (35-day) shelf life. This was a sevenfold increase in the shelf life of sliced poultry sausage compared with the control.

Comparison of the Microflora on Organically and Conventionally Grown Spring Mix from a California Processor

2005 ◽  
Vol 68 (6) ◽  
pp. 1143-1146 ◽  
Author(s):  
CHRISTIE A. PHILLIPS ◽  
MARK A. HARRISON
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Foodborne Pathogens ◽  
Period Analysis ◽  
E Coli ◽  
Psychrotrophic Bacteria ◽  
Microbial Group ◽  
The Mean ◽  
Yeasts And Molds ◽  
Organically Grown

Considerable speculation has occurred concerning the potential for higher numbers of foodborne pathogens on organically grown produce compared with produce not grown organically. The microflora composition of spring mix or mesclun, a mixture of multiple salad ingredients, grown either by organic or conventional means was determined. Unwashed or washed spring mix was obtained from a commercial California fresh-cut produce processor who does not use manure in their cultivation practices. Fifty-four samples of each type of product were supplied over a 4-month period. Analysis included enumeration of total mesophiles, psychrotrophs, coliforms, generic Escherichia coli, lactic acid bacteria, yeasts, and molds. In addition, spring mix was analyzed for the presence of Salmonella and Listeria monocytogenes. The mean populations of mesophilic and psychrotrophic bacteria, yeasts, molds, lactic acid bacteria, and coliforms on conventionally grown spring mix were not statistically different (P > 0.05) from respective mean populations on organically grown spring mix. The mean population of each microbial group was significantly higher on unwashed spring mix compared with the washed product. Of the 14 samples found to contain E. coli, eight were from nonwashed conventional spring mix, one was from washed conventional spring mix, and four were from nonwashed organic spring mix. Salmonella and L. monocytogenes were not detected in any of the samples analyzed.

Growth Rates of Mesophilic Bacteria, Aerobic Psychrotrophic Bacteria, and Lactic Acid Bacteria in Low-Dose–Irradiated Pork

1999 ◽  
Vol 62 (11) ◽  
pp. 1297-1302 ◽  
Author(s):  
RAVEENDRAN J. VENUGOPAL ◽  
JAMES S. DICKSON
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Population ◽  
Vacuum Packaging ◽  
Lag Phase ◽  
Phase Duration ◽  
Psychrotrophic Bacteria ◽  
Gompertz Equation ◽  
Pork Patties ◽  
Descriptive Models

Frozen pork patties, thawed overnight at 0°C or temperature abused through storage at 15°C for 24 h, were packaged using both vacuum and air packaging methods. Immediately after packaging, both sets of patties were irradiated at 0, 0.5, 1, and 2 kGy. All the samples were stored at 2°C and were analyzed for populations of mesophilic, psychrotrophic, and lactic acid bacteria every 3 days for 30 days. By using a mesophilic population of 107 cells/g as a criteria for spoilage, fresh pork patties receiving a dose of 0 kGy had shelf lives of 11 and 16 days with air and vacuum packaging methods, respectively, whereas temperature-abused patties had a shelf life of 7 days with both air and vacuum packaging methods. Both fresh and abused patties that received a dose of 2 kGy had shelf lives that were greater than 30 days at 2°C with both air and vacuum packaging methods. Descriptive models based on the Gompertz equation for mesophilic, psychrotrophic, and lactic acid bacteria were developed, and the generation time and lag-phase duration for each bacterial population were calculated.

scholarly journals Effect of peroxyacetic acid spray and storage temperature on the microbiota and sensory properties of vacuum packed subprimal cuts

2021 ◽  
Author(s):  
Xianqin Yang ◽  
Hui Wang ◽  
Scott Hrycauk ◽  
Mark D. Klassen
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Storage Temperature ◽  
Life Extension ◽  
Rrna Gene ◽  
Peroxyacetic Acid ◽  
Microbial Profiling ◽  
Antimicrobial Interventions ◽  
The Impact

We investigated the impact of peroxyacetic acid (PAA; 200 ppm) spray on the microbiota and shelf life of commercial vacuum packed beef stored at chiller temperatures. Ribeye cuts (n=147) were collected from a local beef plant on the day of production for two consecutive days, with one set collected at the start of work with the PAA spray nozzles turned off (control) and during the routine production with the PAA spray nozzles turned on (PAA) on each day. Packs were stored at 4, 2 and -1°C for up to 34, 104 and 180 days, and sampled at appropriate intervals for sensory assessment, microbial enumeration and microbial profiling by 16S rRNA gene amplicon analysis. Treatment with PAA did not affect the initial meat pH, the initial numbers of total aerobes, lactic acid bacteria or Enterobacteriaceae (p>0.05) before storage; however, it delayed the onset of spoilage by 7, 21 and 54 days at 4, 2 and -1°C, respectively. Square root models of the variation of growth rate with temperature indicated lactic acid bacteria grew faster and Enterobacteriaceae grew slower on PAA treated than not treated meat. Negative associations between pH and deterioration of meat during storage were observed for PAA treated meat. During storage, the microbiota were primarily dominated by Carnobacterium and Lactobacillus/Lactococcus on control meat, but by Leuconostoc on PAA treated meat. Serratia, Yersinia and Clostridium were identified by LEfSe analysis as biomarkers for control meat, the latter of which was found in high abundance in samples that had the highest spoilage scores. IMPORTANCE The findings of this study show that PAA solutions applied at low concentrations under commercial settings positively modulated the meat microbiota. It did not have bactericidal effects for beef subprimals with very low microbial load. However, it differentially impacted the members of the microbiota, which resulted in delayed onset of spoilage of vacuum packed beef subprimal stored at all three temperatures (4, 2 and -1°C). This differential impact could be through one or a combination of the following factors: favoring the growth of lactic acid bacteria which may in turn exert a competitive exclusion that might be due to production of antimicrobial compounds such as organic acids and bacteriocins; exerting synergistic antimicrobial effects with low temperatures against members of Enterobacteriaceae; direct or indirect inhibitory effects against members of clostridia. These findings not only advance our understanding of the microbial ecology of vacuum packed meat stored at chiller temperatures, but also suggest bacteriostatic concentrations of antimicrobial interventions can be explored for shelf life extension.

Microbiological Quality and Production of Botulinal Toxin in Film-Packaged Broccoli, Carrots, and Green Beans

1999 ◽  
Vol 62 (5) ◽  
pp. 499-508 ◽  
Author(s):  
Y.-Y. HAO ◽  
R. E. BRACKETT ◽  
L. R. BEUCHAT ◽  
M. P. DOYLE
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gas Permeability ◽  
Microbiological Quality ◽  
Packaging Material ◽  
Green Beans ◽  
Psychrotrophic Bacteria ◽  
Anaerobic Microorganisms ◽  
Yeasts And Molds

The production of toxin by a 10-strain mixture of proteolytic Clostridium botulinum in fresh produce packaged in polyethylene films with different oxygen permeability was determined. Broccoli florets, shredded carrots, and green beans inoculated with approximately 102 C. botulinum spores per g were placed in bags (1.4 kg per bag) composed of four films with different oxygen transmission rates (OTRs). Broccoli was packaged in bags with OTRs of 3 (7,000 cm3/m2/24 h) and 4 (16,000 cm3/m2/24 h), and green beans were packaged in bags with OTRs of 2 (6,000 cm3/m2/24 h) and 4. Broccoli and green beans in bags were compressed and heat-sealed. Shredded carrots were packaged in bags with OTRs of 1 (3,000 cm3/m2/24 h) and 3 and vacuum-sealed. Produce was stored at 4, 13, and 21°C for up to 27 (broccoli) or 28 (carrots and green bean) days and analyzed periodically. At each sampling time, gas composition within the bags, pH of the produce, microbial populations (total aerobic and anaerobic microorganisms, lactic acid bacteria, psychrotrophic bacteria, yeasts, and molds), and the presence or absence of botulinal toxin were determined. Packaging material affected the quality of vegetables, especially broccoli stored at 4 and 13°C. For example, broccoli was scored as “good” after 22 days at 4°C when it was packaged in film with higher gas permeability (OTR of 4), whereas broccoli appeared to be in “poor” condition when packaged in film with lower gas permeability (OTR of 3). With the exception of lactic acid bacteria, packaging material did not noticeably influence the growth of microorganisms. Lactic acid bacteria grew better in broccoli packaged in bags with an OTR of 3 than in those with an OTR of 4 at all temperatures. Botulinal toxin was detected in broccoli packaged in bags with an OTR of 3 and stored at 13°C for 21 days and in those with an OTR of 4 and 3 and stored at 21°C for 10 days. All toxic samples were visibly spoiled. Toxin was not detected in produce packaged under any other test conditions.

scholarly journals Effect of Essential Oils and Vacuum Packaging on Spoilage-Causing Microorganisms of Marinated Camel Meat during Storage

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2980
Author(s):  
Tareq M. Osaili ◽  
Fayeza Hasan ◽  
Anas A. Al-Nabulsi ◽  
Dinesh Kumar Dhanasekaran ◽  
Reyad Shaker Obaid ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Essential Oils ◽  
Shelf Life ◽  
Bacterial Growth ◽  
Effective Means ◽  
Antimicrobial Effect ◽  
Vacuum Packaging ◽  
Plate Counts ◽  
Camel Meat

The use of essential oils (EOs) and/or vacuum packaging (VP) with meats could increase product shelf-life. However, no studies investigating the effect of EOs and VP on camel meat background microbiota have been conducted previously. The study aimed to analyze the antimicrobial effect of essential oils (EOs) carvacrol (CA), cinnamaldehyde (CI), and thymol (TH) at 1 or 2% plus vacuum packaging (VP) on the growth of spoilage-causing microorganisms in marinated camel meat chunks during storage at 4 and 10 °C. VP is an effective means to control spoilage in unmarinated camel meat (CM) and marinated camel meat (MCM) compared to aerobic packaging (AP). However, after EO addition to MCM, maximum decreases in spoilage-causing microorganisms were observed under AP on day 7. Increasing the temperature from 4 to 10 °C under AP increased the rate of spoilage-causing bacterial growth in CM and MCM; however, EOs were more effective at 10 °C. At 10 °C the maximum reductions in total mesophilic plate counts, yeast and molds, mesophilic lactic Acid bacteria, Enterobacteriaceae, and Pseudomonas spp. were 1.2, 1.4, 2.1, 3.1, and 4.8 log CFU/g, respectively. Incorporating EOs at 2% in MCM, held aerobically under temperature abuse conditions, delayed spoilage.

Coffee residue as a valorization bio-agent for shelf-life extension of lactic acid bacteria under cryopreservation

2020 ◽  
Vol 118 ◽  
pp. 585-590
Author(s):  
In Seong Choi ◽  
Seung Hee Ko ◽  
Ho Myeong Kim ◽  
Jung Eun Yang ◽  
Seul-Gi Jeong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Life Extension ◽  
Shelf Life Extension
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