scholarly journals Cendawan Mikoriza Arbuskula (CMA) Gigaspora spp sebagai pupuk hayati pada pembibitan Mete (Anacardium occidentale L.)

2020 ◽  
Vol 4 (2) ◽  
pp. 193
Author(s):  
Meitini Wahyuni Proborini ◽  
Ida Bagus Gede Daymayasa ◽  
Deny Suhernawan Yusup ◽  
Job Nico Subagio
Keyword(s):  
Anacardium Occidentale

Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
A. Muntala ◽  
P. M. Norshie ◽  
K. G. Santo ◽  
C. K. S. Saba
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Anacardium Occidentale ◽  
Causative Organism ◽  
Pathogenicity Test ◽  
Small Water ◽  
Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

In Silico Molecular Docking and Molecular Dynamic Simulation of Potential Inhibitors of 3C-like Main Proteinase (3CLpro) from Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Using Selected African Medicinal Plants

2020 ◽  
Author(s):  
Sahar Qazi ◽  
Mustafa Alhaji Isa ◽  
Adam Mustapha ◽  
Khalid Raza ◽  
Ibrahim Alkali Allamin ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Severe Acute Respiratory Syndrome ◽  
In Silico ◽  
Md Simulation ◽  
Binding Energies ◽  
Anacardium Occidentale ◽  
Upper Respiratory Tract ◽  
Ligand Complex ◽  
In Silico Docking ◽  
Main Protease

<p>The Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) is an infectious virus that causes mild to severe life-threatening upper respiratory tract infection. The virus emerged in Wuhan, China in 2019, and later spread across the globe. Its genome has been completely sequenced and based on the genomic information, the virus possessed 3C-Like Main Protease (3CLpro), an essential multifunctional enzyme that plays a vital role in the replication and transcription of the virus by cleaving polyprotein at eleven various sites to produce different non-structural proteins. This makes the protein an important target for drug design and discovery. Herein, we analyzed the interaction between the 3CLpro and potential inhibitory compounds identified from the extracts of <i>Zingiber offinale</i> and <i>Anacardium occidentale</i> using in silico docking and Molecular Dynamics (MD) Simulation. The crystal structure of SARS-CoV-2 main protease in complex with 02J (5-Methylisoxazole-3-carboxylic acid) and PEJ (composite ligand) (PDB Code: 6LU7,2.16Å) retrieved from Protein Data Bank (PDB) and subject to structure optimization and energy minimization. A total of twenty-nine compounds were obtained from the extracts of <i>Zingiber offinale </i>and the leaves of <i>Anacardium occidentale. </i>These compounds were screened for physicochemical (Lipinski rule of five, Veber rule, and Egan filter), <i>Pan</i>-Assay Interference Structure (PAINS), and pharmacokinetic properties to determine the Pharmaceutical Active Ingredients (PAIs). Of the 29 compounds, only nineteen (19) possessed drug-likeness properties with efficient oral bioavailability and less toxicity. These compounds subjected to molecular docking analysis to determine their binding energies with the 3CLpro. The result of the analysis indicated that the free binding energies of the compounds ranged between ˗5.08 and -10.24kcal/mol, better than the binding energies of 02j (-4.10kcal/mol) and PJE (-5.07kcal.mol). Six compounds (CID_99615 = -10.24kcal/mol, CID_3981360 = 9.75kcal/mol, CID_9910474 = -9.14kcal/mol, CID_11697907 = -9.10kcal/mol, CID_10503282 = -9.09kcal/mol and CID_620012 = -8.53kcal/mol) with good binding energies further selected and subjected to MD Simulation to determine the stability of the protein-ligand complex. The results of the analysis indicated that all the ligands form stable complexes with the protein, although, CID_9910474 and CID_10503282 had a better stability when compared to other selected phytochemicals (CID_99615, CID_3981360, CID_620012, and CID_11697907). </p>

In Silico Molecular Docking and Molecular Dynamic Simulation of Potential Inhibitors of 3C-like Main Proteinase (3CLpro) from Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Using Selected African Medicinal Plants

2020 ◽  
Author(s):  
Sahar Qazi ◽  
Mustafa Alhaji Isa ◽  
Adam Mustapha ◽  
Khalid Raza ◽  
Ibrahim Alkali Allamin ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Severe Acute Respiratory Syndrome ◽  
In Silico ◽  
Md Simulation ◽  
Binding Energies ◽  
Anacardium Occidentale ◽  
Upper Respiratory Tract ◽  
Ligand Complex ◽  
In Silico Docking ◽  
Main Protease

<p>The Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) is an infectious virus that causes mild to severe life-threatening upper respiratory tract infection. The virus emerged in Wuhan, China in 2019, and later spread across the globe. Its genome has been completely sequenced and based on the genomic information, the virus possessed 3C-Like Main Protease (3CLpro), an essential multifunctional enzyme that plays a vital role in the replication and transcription of the virus by cleaving polyprotein at eleven various sites to produce different non-structural proteins. This makes the protein an important target for drug design and discovery. Herein, we analyzed the interaction between the 3CLpro and potential inhibitory compounds identified from the extracts of <i>Zingiber offinale</i> and <i>Anacardium occidentale</i> using in silico docking and Molecular Dynamics (MD) Simulation. The crystal structure of SARS-CoV-2 main protease in complex with 02J (5-Methylisoxazole-3-carboxylic acid) and PEJ (composite ligand) (PDB Code: 6LU7,2.16Å) retrieved from Protein Data Bank (PDB) and subject to structure optimization and energy minimization. A total of twenty-nine compounds were obtained from the extracts of <i>Zingiber offinale </i>and the leaves of <i>Anacardium occidentale. </i>These compounds were screened for physicochemical (Lipinski rule of five, Veber rule, and Egan filter), <i>Pan</i>-Assay Interference Structure (PAINS), and pharmacokinetic properties to determine the Pharmaceutical Active Ingredients (PAIs). Of the 29 compounds, only nineteen (19) possessed drug-likeness properties with efficient oral bioavailability and less toxicity. These compounds subjected to molecular docking analysis to determine their binding energies with the 3CLpro. The result of the analysis indicated that the free binding energies of the compounds ranged between ˗5.08 and -10.24kcal/mol, better than the binding energies of 02j (-4.10kcal/mol) and PJE (-5.07kcal.mol). Six compounds (CID_99615 = -10.24kcal/mol, CID_3981360 = 9.75kcal/mol, CID_9910474 = -9.14kcal/mol, CID_11697907 = -9.10kcal/mol, CID_10503282 = -9.09kcal/mol and CID_620012 = -8.53kcal/mol) with good binding energies further selected and subjected to MD Simulation to determine the stability of the protein-ligand complex. The results of the analysis indicated that all the ligands form stable complexes with the protein, although, CID_9910474 and CID_10503282 had a better stability when compared to other selected phytochemicals (CID_99615, CID_3981360, CID_620012, and CID_11697907). </p>

SOBREVIVÊNCIA DE ESPÉCIES NATIVAS SOB EFEITOS DE HIGROGEL

2020 ◽  
Vol 3 (3) ◽  
pp. 12
Author(s):  
Maria Rita De Sousa Araújo ◽  
Wanderson Dias Sarmento ◽  
Pedro Alves Dos Santos ◽  
Manuel Caíque Ehrich Rodrigues ◽  
Selma Dos Santos Feitosa
Keyword(s):  
Anacardium Occidentale ◽  
G 12

A disponibilidade hídrica se constitui num fator limitante a sobrevivência de espécies vegetais, especialmente no semiárido nordestino, por possuir prolongadas secas, sendo fundamental o estudo de ações sustentáveis que possam atenuar esses efeitos. O hidrogel é um polímero retentor de umidade que, incorporado ao solo, atua como ferramenta de combate aos efeitos de estresse hídrico, além de melhorar a aeração e drenagem do solo, bem como a redução das perdas de nutrientes por lixiviação. Portanto, objetivou-se investigar a eficiência do hidrogel em espécies nativas ao grau de sobrevivência no Alto Sertão da Paraíba.<strong> </strong>As mudas de <em>Anacardium occidentale </em>e <em>Eugenia pyriformis</em> estão inseridas em um sistema agroflorestal que possui uma área de 3.575m<sup>2</sup>, localizado no Instituto Federal de Educação, Ciência e Tecnologia da Paraíba - Campus Sousa. Foram transplantadas 64 mudas para o sistema onde foram mantidas com regas semanais por 30 dias, para contribuir no processo de aclimatização. Após esse período, aplicou-se o hidrogel, adotou-se delineamento estatístico em bloco ao acaso e quatro repetições em esquema fatorial (4x2+1) sendo oito tratamentos constituídos pelas dosagens: 0 g; 4 g; 8 g; 12 g de hidrogel e pelas duas espécies, e uma testemunha adicional (sem hidrogel com rega). O hidrogel foi aplicado pós-plantio em furos próximos a muda e paralelo às raízes das mesmas. Após a aplicação foi avaliada a Sintomatologia do Estresse Hídrico a cada três dias para identificar o grau de sobrevivência das mudas, onde adotou-se a escala: SEM – planta sem sintomas; PSM – planta com poucos sintomas de murcha; SMM – planta com sintomas moderados; SSM – planta com sintomas severos de murcha. Observou-se que as mudas que receberam 12 g de hidrogel permaneceram durante seis semanas com a maior porcentagem (83%) de plantas sem sintomas de murcha, decaindo apenas na 8ª semana para 68%, fato esse observado em mudas de <em>A. occidentale</em>. Em testemunhas que receberam regas, na 6ª semana, 91% delas não apresentaram sintomas de murcha, decaindo apenas na 8ª semana para 81%. Para <em>E. pyriformis</em>, o tratamento  com maior dosagem de hidrogel, tornou-se o mais eficiente para a cultura, com 12% das plantas sem sintomas de murcha, tendo em vista que durante seis semanas, os demais tratamentos não apresentaram diferença significativa.<strong> </strong>Para mudas que receberam rega, 34% das mesmas não apresentaram sintomas de murcha até a 8ª semana.<strong> </strong>Tendo em vista o percentual de plantas que se estabeleceram durante o período de dois meses com ausência de regas e o uso do hidrogel, observamos que a <em>E. pyriformis </em>apresentou-se mais sensível ao estresse hídrico provocado no sistema, enquanto que para a <em>A. occidentale, </em>a<em> </em>maior dosagem de hidrogel tornou-se significativa quanto ao percentual de murcha, sendo comparada as plantas que receberam regas. Assim, conclui-se que o hidrogel é eficiente em sistemas agroflorestais que passaram por um longo período de estresse hídrico, assegurando a sobrevivência da planta.

scholarly journals Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using aqueous extract from Anacardium occidentale leaf: comparative study of different precursors

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Eric Kwabena Droepenu ◽  
Ebenezer Aquisman Asare ◽  
Boon Siong Wee ◽  
Rafeah Binti Wahi ◽  
Frederick Ayertey ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Comparative Study ◽  
Aqueous Extract ◽  
Zinc Chloride ◽  
Zinc Acetate ◽  
Zinc Acetate Dihydrate ◽  
Inhibition Zone ◽  
Anacardium Occidentale ◽  
Acetate Dihydrate

Abstract Background Various parts of Anacardium occidentale plant possess curative qualities like antidiabetic, anti-inflammatory, antibacterial, antifungal, and antioxidant. Aqueous extract of this plant leaf was used in biosynthesizing zinc oxide (ZnO) nanoaggregates using two precursors of zinc salt (zinc acetate dihydrate [Zn(CH3COO)2∙2H2O] and zinc chloride [ZnCl2]). The synthesized ZnO samples were used in a comparative study to investigate the antibacterial activity against selected Gram-positive and Gram-negative microbes [Staphylococcus aureus, Exiguobacterium aquaticum (Gram +ve) and Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii (Gram −ve)]. The synthesized ZnO nanoaggregates from the two precursors were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive x-ray spectroscopy (EDX) techniques. Results Micrographs of SEM and TEM confirmed nanoparticles agglomerated into aggregates. While spherical nanoaggregates were identified in samples prepared from Zn(CH3COO)2∙2H2O, flake-like structures were identified in samples synthesized from ZnCl2. Particle size determined by TEM was 107.03 ± 1.54 nm and 206.58 ± 1.86 nm for zinc acetate dihydrate and zinc chloride precursors respectively. ZnO nanoaggregate synthesized using zinc acetate as precursor gave higher antibacterial activity than its counterpart, zinc chloride with K. pneumonia recording the highest inhibition zone of 2.08 ± 0.03 mm (67.53%) whereas S. aureus recorded the least inhibition zone of 1.06 ± 0.14 mm (34.75%) for ZnO nanoaggregate from zinc chloride precursor. Also, antibacterial activity increases with increasing concentration of the extract in general. However, A. baumannii, E. aquaticum, and K. pneumoniae did not follow the continuity trend with regards to the 250 ppm and 500 ppm concentrations. Conclusion Biosynthesis of ZnO nanoaggregates using aqueous extract of A. occidentale leaf from zinc acetate dihydrate and zinc chloride as precursors was successful with the formation of nanospheres and nanoflakes. The study suggested that A. occidentale sp. could be an alternative source for the production of ZnO nanoparticles and are efficient antibacterial compounds against both Gram +ve and Gram −ve microbes with its promising effect against infectious bacteria.

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