scholarly journals ENTEROPATHOGENIC ESCHERICHIA COLI STRAINSFROM DIARRHOEIC STOOLSAMPLES OF CHILDREN BELOW 5 YEARS OF AGE IN DAMATURU, YOBE STATE, NIGERIA

2020 ◽  
Vol 8 ◽  
pp. 19
Author(s):  
Anotu Mopelola Deji-Agboola ◽  
Mohammed Ali ◽  
Olubunmi Adetokunbo Osinupebi ◽  
Stephen Olaosebikan Makanjuola
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction Method ◽  
Enteropathogenic Escherichia Coli ◽  
Hygiene Practices ◽  
Cause Of Deaths ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Susceptibility Tests ◽  
Predominant Strain ◽  
Infants And Young Children

Enteropathogenic Escherichia coli (EPEC) is an important cause of deaths mostly in infants and young children with diarrhoea worldwide. This study investigated Enteropathogenic Escherichia coli strains in diarrhoeic stool samples of children below 5 years of age in Damaturu, Yobe State, Nigeria. Microscopy, culture and antibiotic susceptibility tests were carried out on stool specimens obtained from children with diarrhoea. All isolated Escherichia coli were investigated for virulence eae and eaf genes of EPEC strains using Polymerase Chain Reaction method. Information on risk factors of diarrhoea was obtained using the questionnaire. Out of 307 children, 154 (50.2%) were male and 153 (49.8%) female, majority 107 (34.9%) were 3 years old. A total of 175 (57.0%) Escherichia coli were isolated, 19 (10.9%) were identified to be enteropathogenic Escherichia coli of these, 17 (89.5%) were atypical (carries eae genes) while only 2 (10.5%) were typical (harbours eaf genes). Multidrug resistance was observed in some of the isolates, the EPEC were resistant to Reflacin (47.4%), Ciprofloxacin (36.8%), Augmentin (36.8%), Septrin (36.8%). The major factor that predispose children to diarrhoea are poor hygiene practices. Escherichia coli was the most prevalent bacterial causing diarrhoea and atypical EPEC is the predominant strain circulating among these children.

scholarly journals Molecular detection of a new pathotype enteroaggregative haemorrhagic Escherichia coli (EAHEC) in Indonesia, 2015

2020 ◽  
Author(s):  
Wahyu Setyarini ◽  
Dadik Raharjo ◽  
Radita Yuniar Arizandy ◽  
Zakaria Pamoengkas ◽  
Subijanto Marto Sudarmo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction Method ◽  
Unique Combination ◽  
Haemolytic Uremic Syndrome ◽  
E Coli ◽  
Virulence Potential ◽  
Uremic Syndrome ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Enterohemorrhagic E.Coli

Enteroaggregative haemorrhagic Escherichia coli (E. Coli, EAHEC) has been identified as the agent responsible for one of the largest outbreaks of gastroenteritis and Haemolytic-uremic syndrome (HUS) that is transmitted through food in Germany in 2011. The hypervirulent pathotype has a unique combination of two pathogens namely enterohemorrhagic E.coli strain (EHEC) which produces shiga/verotoxin and enteroaggregative E.coli toxins (EAEC) which produces toxins similar to ST and hemolysin. The toxin produced by the EAHEC strain is a hybrid pathotype that combines the virulence potential of the EAEC and EHEC strains that will damage the microcirculation, cause vasculitis and other toxic effects. The purpose of this study was to determine the percentage of samples infected with enteroaggregative hemorrhagic E. coli bacteria (EAHEC) in pediatric diarrhea patients at DR. Soetomo Hospital, Surabaya, Indonesia, 2015. This study used PCR (Polymerase Chain Reaction) method to detect enteroaggregative E. coli strains (CVD432 and aaic genes) and enterohemorrhagic E.coli (eae gene).The results showed that 33 out of 40 (82,5%) stool samples examined were detected enteroaggregative E. coli (EAEC), 4 out of 40 (10%) enterohemorrhagic E. coli (EHEC) and 3 out of 40 (7,5%) enteroaggregative haemorrhagic E. coli bacteria (EAHEC) , which caused diarrhea in pediatric diarrhea patients at Dr. Soetomo General Hospital. The unique combination of genomic features of the Surabaya outbreak strain, containing characteristics from pathotypes EAEC and EHEC, suggested that it represents a new pathotype enteroaggregative haemorrhagic E. coli (EAHEC). It is expected that development of specific primer design and sequencing are needed to continue in this research.

scholarly journals Haemolytic uraemic syndrome and Shiga toxin-producing Escherichia coli infection in children in France

2000 ◽  
Vol 124 (2) ◽  
pp. 215-220 ◽  
Author(s):  
B. DECLUDT ◽  
P. BOUVET ◽  
P. MARIANI-KURKDJIAN ◽  
F. GRIMONT ◽  
P. A. D. GRIMONT ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Prospective Study ◽  
Shiga Toxin ◽  
Haemolytic Uraemic Syndrome ◽  
Serum Samples ◽  
E Coli ◽  
Escherichia Coli Infection ◽  
Stool Samples ◽  
Polymerase Chain

We conducted a study to determine the incidence of haemolytic uraemic syndrome (HUS) in children in France and to assess the role of Shiga-toxin-producing Escherichia coli (STEC) infection in the aetiology of HUS. In collaboration with the Société de Néphrologie Pédiatrique we undertook a retrospective review of all cases of HUS hospitalized from January 1993 to March 1995 and a 1-year prospective study (April 1995–March 1996) of epidemiological and microbiological features of cases of HUS. The polymerase chain reaction (PCR) procedure was used to detect stx, eae, e-hlyA genes directly from case stool samples. Serum samples from cases were examined for antibodies to lipopolysaccharide (LPS) of 26 major STEC serogroups. Two hundred and eighty-six cases were reported. The average incidence per year was 0·7/105 children < 15 years and 1·8/105 children < 5 years. During the prospective study, 122/130 cases were examined for evidence of STEC infection using PCR and/or serological assays and 105 (86%) had evidence of STEC infection. Serum antibodies to E. coli O157 LPS were detected in 79 (67%) cases tested. In conclusion, this study showed that STEC infection is an important cause of HUS in children in France, with a high proportion related to the O157 serogroup.

Establishment of a Polymerase Chain Reaction Method for Detection of Escherichia coli in Amniotic Fluid in Patients with Chorioamnionitis

2008 ◽  
Vol 24 (2) ◽  
pp. 132-139 ◽  
Author(s):  
Ghada A. Daoud ◽  
Yoshikatsu Suzuki ◽  
Tamao Yamamoto ◽  
Takashi Suzuki ◽  
Nobuhiro Suzumori ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Amniotic Fluid ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

scholarly journals Prevalence, phylogeny, and antimicrobial resistance of Escherichia coli pathotypes isolated from children less than 5 years old with community acquired- diarrhea in Upper Egypt

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Rasha M. M. Khairy ◽  
Zahra Atef Fathy ◽  
Doaa Mohamed Mahrous ◽  
Ebtisam S. Mohamed ◽  
Soha S. Abdelrahim
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance Genes ◽  
E Coli ◽  
Extended Spectrum ◽  
Causative Agents ◽  
Typical Epec ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Lactamase Gene ◽  
Children Under 5

Abstract Background Diarrhoea, affecting children in developing countries, is mainly caused by diarrheagenic Escherichia coli (DEC). This study principally aimed to determine the prevalence of DEC pathotypes and Extended-spectrum β-lactamase (ESBL) genes isolated from children under 5 years old with diarrhea. Methods A total of 320 diarrhoea stool samples were investigated. E. coli isolates were investigated for genes specific for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC) and enterohemorrhagic E. coli (EHEC) using polymerase chain reaction (PCR). Furthermore, antimicrobial susceptibility testing, detection of antibiotic resistance-genes and phylogenetic typing were performed. Results Over all, DEC were isolated from 66/320 (20.6%) of the children with diarrhoea. EAEC was the predominant (47%), followed by typical EPEC (28.8%) and atypical EPEC (16.6%). Co-infection by EPEC and EAEC was detected in (7.6%) of isolates. However, ETEC, EIEC and EHEC were not detected. Phylogroup A (47%) and B2 (43.9%) were the predominant types. Multidrug-resistance (MDR) was found in 55% of DEC isolates. Extended-spectrum β-lactamase (ESBL) genes were detected in 24 isolates (24 blaTEM and 15 blaCTX-M-15). Only one isolate harbored AmpC β-lactamase gene (DHA gene). Conclusion The study concluded that, EAEC and EPEC are important causative agents of diarrhoea in children under 5 years. MDR among DEC has the potential to be a big concern.

Molecular detection of Escherichia coli (E. coli) from Diarrheal stool samples from children in Quetta, Balochistan, Pakistan.

2019 ◽  
Vol 2 (2) ◽  
pp. 27-31
Author(s):  
Tauseef M Asmat
Keyword(s):  
Escherichia Coli ◽  
Developing Countries ◽  
Disease Transmission ◽  
Causes Of Death ◽  
Growth Media ◽  
Huge Number ◽  
E Coli ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Control Disease

Diarrhea is one of the major causes of death in children, particularly in developing countries. Rapid detection and treatment is necessary to control disease transmission in the community and thus limiting the huge number of death toll. The major cause of diarrhea in developing countries is Escherichia coli (E. coli).This study was aimed to isolate E. coli from diarrheal stool samples from children aged 05 months to 05 years visited/ hospitalized in Quetta due to acute/persistent diarrhea. Diarrheal stool samples from 200 children were collected from Lady Sandeman Hospital Quetta and cultured on nutrient agar and later transferred to E. coli specific growth media for initial detection. For further confirmation the colonies were subjected to polymerase chain reaction (PCR). The PCR results revealed that 44(22%) samples out of 200 samples were positive for E. coli. These results indicate a high proportion of E. coli infection among children suffering with diarrhea.

Detection of the mcr-1 gene in Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains isolated from broilers

2020 ◽  
Vol 40 (3) ◽  
pp. 165-169
Author(s):  
Hugo P. Lopes ◽  
Gisllany A. Costa ◽  
Ana C.L.Q. Pinto ◽  
Leandro S. Machado ◽  
Nathalie C. Cunha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Poultry Products ◽  
Drug Of Choice ◽  
Bacteriological Method ◽  
Enteric Infections ◽  
Polymerase Chain

ABSTRACT: Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains are among the major pathotypes found in poultry and their products, which are capable of causing human enteric infections. Colistin has been claimed the drug of choice against diseases caused by multidrug-resistant Gram-negative bacteria (MDRGN) in humans. The mcr-1 gene was the first plasmidial gene that has been described to be responsible for colistin resistance and has also been detected in birds and poultry products. Our study aimed to detect the mcr-1 gene in enteropathogenic strains of E. coli in order to evaluate the resistance to colistin in broilers. The material was obtained from 240 cloacal samples and 60 broiler carcasses. The strains were isolated by the conventional bacteriological method and by the virulence genes, which characterize the enteropathogenic strains and resistance, and the samples were detected by polymerase chain reaction (PCR). Of the 213 isolated strains of E. coli, 57 (26.76%) were characterized as atypical EPEC and 35 (16.43%) as STEC. The mcr-1 gene was found in 3.5% (2/57) of the EPEC strains and 5.7% (2/35) of the STEC strains. In this study, it was possible to confirm that the mcr-1 resistance gene is already circulating in the broiler flocks studied and may be associated with the pathogenic strains.

scholarly journals Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Kaewkanya Nakjarung ◽  
Ladaporn Bodhidatta ◽  
Pimmnapar Neesanant ◽  
Paphavee Lertsethtakarn ◽  
Orntipa Sethabutr ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Young Children ◽  
Real Time ◽  
Pediatric Hospital ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Phnom Penh ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Predominant Strain

This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006. In the previous study, 926 stool samples were collected from children aged 3–60 months with acute diarrhea (cases) and without diarrhea (controls) with reported 6.7% of cases and 3.2% of controls being positive for norovirus. The initial norovirus diagnostic assay was performed with real-time reverse transcription-polymerase chain reaction (real-time RT PCR) which also distinguished between genogroups I and II (GI and GII). Norovirus infection was most commonly detected in children aged 12–23 months in both cases and controls. Norovirus Genotyping Tool and phylogenetic analysis of partial sequences of the 3′ end of the RNA-dependent RNA Polymerase (RdRp) and the capsid domain region were employed to assign genotypes of the norovirus strains. GII.4 was the most predominant capsid genotype detected at 39.5% followed by GII.6 at 14.9%. The GII.4 Hunter 2004 variant was the predominant strain detected. Six RdRP/capsid recombinants including GII.P7/GII.6, GII.P7/GII.14, GII.P7/GII.20, GII.P12/GII.13, GII.P17/GII.16, and GII.P21/GII.3 were also identified. This study of norovirus infection in young children in Cambodia suggests genetic diversity of norovirus as reported worldwide.

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