scholarly journals Isolation and Selection of some Antagonizing Fungal Strains of Trichoderma And Chaetonium that can Inhibit the Growth of Pathogenic Fungi on Orange Trees

2021 ◽  
Vol 07 (09) ◽  
Author(s):  
Le Minh Thanh ◽  
Keyword(s):  
Soil Fungi ◽  
Pathogenic Fungi ◽  
Trichoderma Asperellum ◽  
Long Time ◽  
Decline Disease ◽  
Microbial Strains ◽  
Diseased Root ◽  
Orange Trees ◽  
Selection Of

Decline disease has been discovered for a long time but infection is more extensively increasing. It is difficult to detect because the disease is originated from roots. Among the causes, soil fungi have been widely determined. Using fungicides is not an effective way to control the disease. In this case, biocontrol with suitable microbial strains is a potential approach. This study aims to investigate in vitro the possibility of using Trichoderma and Chaetonium to control the causing fungi. Two Chaetonium strains and 1 Trichoderma asperellum strain were isolated from diseased-root samples. Six strains of Trichoderma (isolated strains T1, T2, T3, T4, T5, T6) and three strains of Chaetonium (isolated strains C1, C2, C3) showed a reasonably antagonistic ability to Phytophthora, Fusarium, Rhizoctonia. Base on the PCR identification method, 6 strains of Trichoderma was isolated belonging to Trichoderma asperellum species, and 3 strains of Chaetonium belonging to 2 species Chaetomium globosum and Chaetomium cichlids.

scholarly journals Selection of antagonists for biocontrol of Xanthomonas euvesicatoria

2020 ◽  
pp. 181-189
Author(s):  
Ivana Pajcin ◽  
Vanja Vlajkov ◽  
Dragoljub Cvetkovic ◽  
Maja Ignjatov ◽  
Mila Grahovac ◽  
...  
Keyword(s):  
Biological Control ◽  
Plant Disease ◽  
Plant Pathogens ◽  
Plant Diseases ◽  
Bacterial Spot ◽  
Cultivation Medium ◽  
Microbial Strains ◽  
Xanthomonas Euvesicatoria ◽  
Selection Of

Xanthomonas euvesicatoria is a worldwide causer of pepper bacterial spot, a bacterial plant disease responsible for massive losses of fresh pepper fruits. Considering the current problems in management of bacterial plant diseases, biological control using antagonistic microbial strains with high potential for plant pathogens suppression emerges as a possible solution. The aim of this study was to select suitable antagonists for suppression of X. euvesicatoria among the bacteria, yeast and fungi from the genera Pseudomonas, Lactobacillus, Saccharomyces and Trichoderma, based on in vitro antimicrobial activity testing using the diffusion disc method. The results of this study have revealed that cultivation broth samples of the antagonists Lactobacillus MK3 and Trichoderma reseii QM 9414, as well as supernatant samples of the antagonist Pseudomonas aeruginosa I128, have showed significant potential to be applied in biological control of X. euvesicatoria. Further research would be required to formulate suitable cultivation medium and optimize bioprocess conditions for production of the proposed pepper bacterial spot biocontrol agents.

scholarly journals Synergistic antifungal interactions of amphotericin B with 4-(5-methyl-1,3,4-thiadiazole-2-yl) benzene-1,3-diol

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Barbara Chudzik ◽  
Katarzyna Bonio ◽  
Wojciech Dabrowski ◽  
Daniel Pietrzak ◽  
Andrzej Niewiadomy ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Synergistic Interaction ◽  
Clinical Isolates ◽  
Screening Tests ◽  
Synergistic Interactions ◽  
Low Toxicity ◽  
Selection Of

Abstract Amphotericin B (AmB) is a very potent antifungal drug with very rare resistance among clinical isolates. Treatment with the AmB formulations available currently is associated with severe side effects. A promising strategy to minimize the toxicity of AmB is reducing its dose by combination therapy with other antifungals, showing synergistic interactions. Therefore, substances that display synergistic interactions with AmB are still being searched for. Screening tests carried out on several dozen of synthetic 1,3,4-thiadiazole derivatives allowed selection of a compound called 4-(5-methyl-1,3,4-thiadiazole-2-yl) benzene-1,3-diol (abbreviated as C1), which shows strong synergistic interaction with AmB and low toxicity towards human cells. The aim of the present study was to investigate the type of in vitro antifungal interactions of the C1 compound with AmB against fungal clinical isolates differing in susceptibility. The results presented in the present paper indicate that the C1 derivative shows strong synergistic interaction with AmB, which allows the use of a dozen to several dozen times lower AmB concentration necessary for 100% inhibition of the growth of pathogenic fungi in vitro. Synergistic interactions were noted for all tested strains, including strains with reduced sensitivity to AmB and azole-resistant isolates. These observations give hope for the possibility of application of the AmB - C1 combinatory therapy in the treatment of fungal infections.

Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

SISTEM PENDUKUNG KEPUTUSAN PEMILIHAN KEPALA CABANG TERBAIK BANK SUMUT DENGAN MENERAPKAN METODE VIKOR

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Betrik J Hutapea ◽  
Mesran Mesran ◽  
Siti Nurhabibah
Keyword(s):  
Decision Support ◽  
Decision Support System ◽  
Support System ◽  
Development Bank ◽  
Vikor Method ◽  
The North ◽  
Long Time ◽  
North Sumatra ◽  
Regional Development Bank ◽  
Selection Of

SUMUT Bank is one of the Banks in Indonesia with the name of the company PT. Regional Development Bank of North Sumatra. The North Sumatra Bank has branches in each region in North Sumatra both in the district and in the sub-district, and each of these branches is led by a branch leader or branch head. The head of this branch is responsible for the reversal of the Bank being led. The best and most accomplished branch heads deserve more and more awards. The selection of the best branch heads is selected transparently and structured in the hope that it can be a motivation for all branch heads to be able to further improve the quality and service of the Bank they lead. Making the best branch head selection done manually will take a long time and tends to be less transparent and structured. One solution so that the implementation of the selection can be carried out easily and quickly, it requires a Decision Support System that can provide consistency of assessment. In this study the method used is the VIKOR method (Visekriterijumsko Kompromisno Rangiranje). This method makes cracking on alternatives based on criteria that have been determined with an ideal compromise solution or the best solution, so that this system can later be beneficial for the SUMUT Bank to get the title in determining the best branch head.Keywords: Decision Support System, North Sumatra Bank, Branch Head, Vikor

Sign in / Sign up

Export Citation Format

Share Document