scholarly journals In vitro ASSESSMENT OF Trichoderma asperellum ISOLATED FROM PLANT RHIZOSPHERE AND EVALUATION OF THEIR POTENTIAL ACTIVITY AGAINST SOME PATHOGENIC FUNGI

2016 ◽  
Vol 45 (1) ◽  
pp. 113-128
Author(s):  
G HASSAN ◽  
NADA HEMEDA
Keyword(s):  
Pathogenic Fungi ◽  
Trichoderma Asperellum ◽  
Potential Activity ◽  
In Vitro Assessment

scholarly journals Isolation and Selection of some Antagonizing Fungal Strains of Trichoderma And Chaetonium that can Inhibit the Growth of Pathogenic Fungi on Orange Trees

2021 ◽  
Vol 07 (09) ◽  
Author(s):  
Le Minh Thanh ◽  
Keyword(s):  
Soil Fungi ◽  
Pathogenic Fungi ◽  
Trichoderma Asperellum ◽  
Long Time ◽  
Decline Disease ◽  
Microbial Strains ◽  
Diseased Root ◽  
Orange Trees ◽  
Selection Of

Decline disease has been discovered for a long time but infection is more extensively increasing. It is difficult to detect because the disease is originated from roots. Among the causes, soil fungi have been widely determined. Using fungicides is not an effective way to control the disease. In this case, biocontrol with suitable microbial strains is a potential approach. This study aims to investigate in vitro the possibility of using Trichoderma and Chaetonium to control the causing fungi. Two Chaetonium strains and 1 Trichoderma asperellum strain were isolated from diseased-root samples. Six strains of Trichoderma (isolated strains T1, T2, T3, T4, T5, T6) and three strains of Chaetonium (isolated strains C1, C2, C3) showed a reasonably antagonistic ability to Phytophthora, Fusarium, Rhizoctonia. Base on the PCR identification method, 6 strains of Trichoderma was isolated belonging to Trichoderma asperellum species, and 3 strains of Chaetonium belonging to 2 species Chaetomium globosum and Chaetomium cichlids.

scholarly journals An in vitro assessment of anti-SARS-CoV-2 activity of oral preparations of iodine complexes (RENESSANS)

2020 ◽  
Author(s):  
Imran Altaf ◽  
Muhammad Faisal Nadeem ◽  
Nadir Hussain ◽  
Muhammad Nawaz ◽  
Sohail Raza ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Cell Survival ◽  
Cytopathic Effect ◽  
Vero Cells ◽  
Iodine Complex ◽  
Oral Formulations ◽  
Potential Activity ◽  
In Vitro Assessment ◽  
Iodine Complexes

ABSTRACTSince the emergence of CoVID-19 pandemic in China in late 2019, scientists are striving hard to explore non-toxic, viable anti-SARS-CoV-2 compounds or medicines. We determined In Vitro anti-SARS-CoV-2 activity of oral formulations (syrup and capsule) of an Iodine-complex (Renessans). A monolayer of vero cells were exposed to SARS-CoV-2 in the presence and absence of different concentrations (equivalent to 50, 05 and 0.5 μg/ml of I2) of Renessans. Anti-SARS-CoV-2 activity of each of the formulation was assessed in the form of cell survival, SARS-CoV-2-specific cytopathic effect (CPE) and genome quantization. With varying concentrations of syrup and capsule, a varying rate of inhibition of CPE, cells survival and virus replication was observed. Compared to 0.5 μg/ml concentration of Renessans syrup, 5 and 50 μg/ml showed comparable results where there was a 100% cell survival, no CPEs and a negligible viral replication (ΔCT= 0.11 and 0.13, respectively). This study indicates that Renessans, containing iodine, may have potential activity against SARS-CoV-2 which needs to be further investigated in human clinical trials.

scholarly journals Virtual Screening and the In Vitro Assessment of the Antileishmanial Activity of Lignans

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2281 ◽  
Author(s):  
Mayara dos Santos Maia ◽  
Joanda Paolla Raimundo e Silva ◽  
Thaís Amanda de Lima Nunes ◽  
Julyanne Maria Saraiva de Sousa ◽  
Gabriela Cristina Soares Rodrigues ◽  
...  
Keyword(s):  
Predictive Models ◽  
Leishmania Major ◽  
Biological Activities ◽  
Computer Aided Drug Design ◽  
Combined Analysis ◽  
Ic50 Value ◽  
Potential Activity ◽  
In Vitro Assessment ◽  
Using Data

Leishmaniasis is endemic in at least 98 countries. Due to the high toxicity and resistance associated with the drugs, we chose lignans as an alternative, due to their favorable properties of absorption, distribution, metabolism, excretion, and toxicity (ADMET). To investigate their leishmanicidal potential, the biological activities of a set of 160 lignans were predicted using predictive models that were built using data for Leishmania major and L. (Viannia) braziliensis. A combined analysis, based on ligand and structure, and several other computational approaches were used. The results showed that the combined analysis was able to select 11 lignans with potential activity against L. major and 21 lignans against L. braziliensis, with multitargeting effects and low or no toxicity. Of these compounds, four were isolated from the species Justicia aequilabris (Nees) Lindau. All of the identified compounds were able to inhibit the growth of L. braziliensis promastigotes, with the most active compound, (159) epipinoresinol-4-O-β-d-glucopyranoside, presenting an IC50 value of 5.39 µM and IC50 value of 36.51 µM for L. major. Our findings indicated the potential of computer-aided drug design and development and demonstrated that lignans represent promising prototype compounds for the development of multitarget drugs against leishmaniasis.

Synthesis and Antimicrobial Activity of Calycanthaceous Derivatives

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110326
Author(s):  
Rui Zhu ◽  
Cheng Yang ◽  
Ke Han ◽  
Yongdong Gu ◽  
Jiwen Zhang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Plant Pathogen ◽  
Antifungal Agent ◽  
Inhibitory Concentration ◽  
Pathogenic Fungi ◽  
Effective Activity ◽  
Potential Activity ◽  
Further Development

A series of calycanthaceous alkaloid analogs have been synthesized in excellent yields. All the target compounds were evaluated in vitro for biological activity against a broad range of plant pathogen fungi, bacteria and human pathogenic fungi, and some of the designed compounds exhibited potential activity in the primary assays. Notably, Compound b7 illustrated higher degrees of activity against Aspergillu sflavu than amphotericin B, with a minimal inhibitory concentration value of 15.63 µg·mL−1. Compound b7 displayed the most effective activity among the tested calycanthaceous analogs and might be a novel potential leading compound for further development of antifungal agent.

In vitro assessment of Labisia pumila and its constituents for interactions with CYPs, P-GP and PXR

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
VK Manda ◽  
OR Dale ◽  
C Awortwe ◽  
Z Ali ◽  
IA Khan ◽  
...  
Keyword(s):  
In Vitro Assessment ◽  
Labisia Pumila

Improved Production of Two Anti-Candida Lipopeptide Homologues Co- Produced by the Wild-Type Bacillus subtilis RLID 12.1 under Optimized Conditions

2020 ◽  
Vol 21 (5) ◽  
pp. 438-450
Author(s):  
Ramya Ramchandran ◽  
Swetha Ramesh ◽  
Anviksha A ◽  
RamLal Thakur ◽  
Arunaloke Chakrabarti ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pathogenic Fungi ◽  
Fold Increase ◽  
Production Yield ◽  
Yield Enhancement ◽  
Bioactive Metabolites ◽  
Candida Auris ◽  
Media Formulation ◽  
Static Conditions

Background:: Antifungal cyclic lipopeptides, bioactive metabolites produced by many species of the genus Bacillus, are promising alternatives to synthetic fungicides and antibiotics for the biocontrol of human pathogenic fungi. In a previous study, the co- production of five antifungal lipopeptides homologues (designated as AF1, AF2, AF3, AF4 and AF5) by the producer strain Bacillus subtilis RLID 12.1 using unoptimized medium was reported; though the two homologues AF3 and AF5 differed by 14 Da and in fatty acid chain length were found effective in antifungal action, the production/ yield rate of these two lipopeptides determined by High-Performance Liquid Chromatography was less in the unoptimized media. Methods:: In this study, the production/yield enhancement of the two compounds AF3 and AF5 was specifically targeted. Following the statistical optimization (Plackett-Burman and Box-Behnken designs) of media formulation, temperature and growth conditions, the production of AF3 and AF5 was improved by about 25.8- and 7.4-folds, respectively under static conditions. Results:: To boost the production of these two homologous lipopeptides in the optimized media, heat-inactivated Candida albicans cells were used as a supplement resulting in 34- and 14-fold increase of AF3 and AF5, respectively. Four clinical Candida auris isolates had AF3 and AF5 MICs (100 % inhibition) ranging between 4 and 16 μg/ml indicating the lipopeptide’s clinical potential. To determine the in vitro pharmacodynamic potential of AF3 and AF5, time-kill assays were conducted which showed that AF3 (at 4X and 8X concentrations) at 48h exhibited mean log reductions of 2.31 and 3.14 CFU/ml of C. albicans SC 5314, respectively whereas AF5 at 8X concentration showed a mean log reduction of 2.14 CFU/ml. Conclusion:: With the increasing threat of multidrug-resistant yeasts and fungi, these antifungal lipopeptides produced by optimized method promise to aid in the development of novel antifungal that targets disease-causing fungi with improved efficacy.

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