scholarly journals Effect of Nonsurgical Periodontal Therapy on Some Oxidative Stress Markers in Patients with Chronic Periodontitis: A Biochemical Study

2013 ◽  
Vol 4 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Tabita Benjamin ◽  
Abdul S Aziz ◽  
Madhav G Kalekar ◽  
Adinath N Suryakar ◽  
Milsee M Prakashan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Chronic Periodontitis ◽  
Biochemical Study ◽  
Periodontal Therapy ◽  
Healthy Controls ◽  
Oxidative Stress Markers ◽  
Clinical Attachment Loss ◽  
Stress Markers ◽  
Nonsurgical Periodontal Therapy

ABSTRACT Introduction Chronic periodontitis is the inflammatory disease of the periodontium; the tissue that surround and support the teeth. The aim of the study was to estimate and compare some biochemical oxidative stress markers in patients with chronic periodontitis and healthy controls and to assess the effect of nonsurgical periodontal therapy on these parameters. Materials and methods Eighty-two chronic periodontitis patients and 120 healthy controls were recruited for the study. The periodontal status was evaluated using gingival index (GI), plaque index (PI), papillary bleeding index (PBI) and clinical attachment loss (CAL). The biochemical parameters estimated were total antioxidant capacity (TAOC), antioxidant enzymes RBC-superoxide dismutase (RBC-SOD) and glutathione peroxidase (GPx), vitamin C, malondialdehyde (MDA) and Creactive protein (CRP) were also evaluated. Nonsurgical periodontal therapy (scaling and root planing; SRP) was performed on chronic periodontitis patients and a follow-up was done after 3 months. Results The study suggests that oxidative stress is induced in chronic periodontitis, as the levels of CRP, MDA and RBC-SOD were significantly higher (p < 0.001) and those of TAOC, GPx and vitamin C were significantly lower (p < 0.001) compared to healthy controls at baseline. SRP therapy significantly improved the clinical and biochemical oxidative stress markers in periodontitis patients. Conclusion Chronic periodontitis patients show higher inflammatory manifestations and oxidative stress. SRP therapy helps in lowering inflammatory burden and improving systemic oxidant: Antioxidant imbalance. The SRP therapy may be a useful support in the control and prevention of various inflammatory diseases associated with periodontitis. How to cite this article Aziz AS, Kalekar MG, Benjamin T, Suryakar AN, Prakashan MM, Bijle MNA. Effect of Nonsurgical Periodontal Therapy on Some Oxidative Stress Markers in Patients with Chronic Periodontitis: A Biochemical Study. World J Dent 2013;4(1):17-23.

Relationship between atherogenic index and oxidative stress among patients presented with coronary artery disease in a tertiary care hospital

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2807-2813
Author(s):  
Resmi C R ◽  
Kedari G S R ◽  
Deepa P K
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Density Lipoprotein ◽  
Atherogenic Index ◽  
Enzymatic Antioxidants ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Lipid Parameters ◽  
And Oxidative Stress

CAD is recognized as a multifactorial disease that is influenced by environmental and genetic factors. This study aimed to evaluate the levels of lipid parameters, oxidative stress and antioxidant markers in subjects with CAD compared to their age & sex matched controls and to analyze the relationship between atherogenic Index and oxidative stress among them 62 clinically proved CAD patients and 62 healthy age and sex matched subjects without CAD were selected for this study. 5 ml of fasting venous blood was collected from all the subjects and investigations such as FPG, lipid profile, oxidative markers Malondialdehyde (MDA), F2 isoprostanes (F2iso) and antioxidants glutathione S-transferase (GST), superoxide dismutase (SOD), vitamin-C, vitamin-E were performed. This study showed that levels of lipid parameters total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c) and AI were significantly higher whereas high density lipoprotein cholesterol (HDL-c) were significantly low in CAD patients compared to normal controls. Oxidative stress markers MDA and F2 Isoprostanes level were significantly high, whereas enzymatic antioxidants GST and SOD and non-enzymatic antioxidants Vitamin-C and Vitamin-E levels were significantly low in CAD patients. Oxidative stress markers were found to significantly influence the AI. Results of this study showed that oxidative stress markers F2iso and MDA and antioxidants GST, VIT-C and VIT-E are found to influence the atherogenic index significantly.

Gingival crevicular fluid and plasma oxidative stress markers and TGM-2 levels in chronic periodontitis

2017 ◽  
Vol 83 ◽  
pp. 47-54 ◽  
Author(s):  
Sema Becerik ◽  
Veli Özgen Öztürk ◽  
Peter Celec ◽  
Natalia Kamodyova ◽  
Gül Atilla ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Crevicular Fluid

scholarly journals Effect of Nonsurgical Periodontal Therapy on Crevicular Fluid and Serum Glutathione Peroxidase Levels

2012 ◽  
Vol 32 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Swati Pradeep Patel ◽  
Nishanth S. Rao ◽  
A. R. Pradeep
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Periodontal Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pocket Depth ◽  
Nonsurgical Periodontal Therapy ◽  
Crevicular Fluid

Background: Plasma glutathione peroxidase (eGPx) is an important selenium containing antioxidant in human defense against oxidative stress. While crevicular fluid (GCF) eGPx levels and its association with periodontal disease is well documented, there is no data on correlation of GCF and serum eGPx levels in chronic periodontitis. Hence this study was undertaken to further probe into the role of oxidative stress in periodontal diseases and effect of nonsurgical periodontal therapy (NSPT) by correlating GCF and serum levels of eGPx.Materials and methods: Thirty subjects (16-Males and 14-Females; age: 30–38 years) participated in the study. The subjects were divided, based on gingival index, probing pocket depth and clinical attachment level into: Healthy (group-1,n=10), Gingivitis (group-2,n=10) and Periodontitis (group-3,n=10). Chronic periodontitis patients after NSPT constituted group 4. GCF and serum samples collected from each subject were quantified for eGPx levels using Enzyme linked Immunosorbent Assay.Results: The mean eGPx concentrations increased from health (14.01 ng/μl and 78.26 ng/ml) to gingivitis (22.86 ng/μl and 90.44 ng/ml) and then to periodontitis (29.89 ng/μl and 103.43 ng/ml), in GCF and serum respectively. After NSPT, there was statistically significant reduction in eGPx concentration in GCF and serum (19.41 ng/μl and 85.21 ng/ml). Further, all the GCF eGPx values showed a positive correlation to that of serum eGPx level.Conclusion: Thus, increased eGPx concentration in GCF can be considered as an indicator of local increase in oxidative stress. While, increase in serum eGPx levels indicates that periodontal disease can also lead to increased oxidative stress at the systemic level.

scholarly journals Sequential Analysis of Oxidative Stress Markers and Vitamin C Status in Acute Bacterial Osteomyelitis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Rade Grbic ◽  
Dijana J Miric ◽  
Bojana Kisic ◽  
Ljiljana Popovic ◽  
Vojkan Nestorovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Sequential Analysis ◽  
Bone Cells ◽  
Redox Status ◽  
Oxidative Stress Markers ◽  
Total Vitamin ◽  
Baseline Serum ◽  
Stress Markers ◽  
Systemic Oxidative Stress

In bacterial bone infections, excessively formed oxidants may result in local and systemic oxidative stress. Vitamin C is the major extracellular nonenzymatic antioxidant, also implicated in bone cells metabolism and viability. The physiological functions of vitamin C largely depend on its redox status. We sequentially assessed oxidative stress markers, hydroperoxides and malondialdehyde (MDA), total antioxidant activity (AOA), total vitamin C, ascorbic acid (Asc), and oxidized/reduced vitamin C ratio in 137 patients with acute osteomyelitis (OM). Compared to 52 healthy controls, in OM group baseline serum hydroperoxides, MDA and oxidized/reduced vitamin C ratio were higher whilst Asc and AOA were lower (P < 0.05, resp.). On the other side, total vitamin C levels in patients and controls were similar(P > 0.05), thereby suggesting a relative rather than absolute vitamin C deficiency in OM. During the follow-up, oxidative stress markers, AOA, and oxidizedreduced vitamin C ratio were gradually returned to normal, while there was no apparent change of total vitamin C concentrations. Persistently high values of oxidized/reduced vitamin C ratio and serum MDA were found in subacute OM. In conclusion, acute OM was associated with enhanced systemic oxidative stress and the shift of vitamin C redox status towards oxidized forms.

scholarly journals Oxidative Stress Markers (8-Isoprostane and 8-Hydroxy-2-Deoxyguanosine) in Major Depression: A Case-control Study

2021 ◽  
Author(s):  
Parul Chopra ◽  
Rajesh Sagar ◽  
Asok Kumar Mukhopadhyay
Keyword(s):  
Oxidative Stress ◽  
Major Depression ◽  
Oxidative Damage ◽  
Case Control Study ◽  
Case Control ◽  
Clinical Severity ◽  
Healthy Controls ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Control Study

Introduction: Depression is associated with activation of innate immune response leading to oxidative damage. The 8-isoprostanes and 8-hydroxy-2-deoxyguanosine (8-OHdG) are biomarkers of oxidative damage to lipids and Deoxyribonucleic Acid (DNA), respectively. They have been independently linked to depression. Aim: To study the oxidative stress markers (8-Isoprostanes and 8-OHdG) in subjects with major depression. Materials and Methods: In this observational case-control study 42 cases of depression, 13-25 years of age were recruited from Psychiatry Out Patient Department (OPD) at a tertiary-care hospital in Delhi, India, along with 42 healthy controls. They were assessed clinically and using psychometric evaluation scores, Beck’s Depression Inventory-II (BDI-II) and Hamilton Depression Rating Scale (HAM-D). All 42 subjects were on medication with antidepressants {33/42 with Selective Serotonin Reuptake Inhibitors (SSRI) 8/42 with Tricyclic Antidepressants (TCA) and 1/42 on a combination of both}. Routine laboratory investigations were done. Plasma 8-Isoprostane and serum 8-OHdG concentrations were measured in both cases and controls. The results obtained were analysed using relevant statistical tests on STATA version 11 (StataCorp, 2009). Results: Clinically, all patients had moderate to severe depression. BDI-II and HAM-D scores were raised in all cases as compared to the controls (28.81±5.60 vs 1.62±1.59 for BDI and 20.88±4.67 vs 1.33±1.43 for HAM-D, respectively). The concentration (in depressed vs controls) of plasma 8-Isoprostane (107.70±54.48 pg/mL vs 77.78±60.15 pg/mL) and serum 8-OHdG (2103.03±154 pg/mL vs 2017±164.69 pg/mL) were significantly elevated (p-value <0.05). Though elevated in patients belonging to both genders, showed significant increase of 8-Isoprostane only in females and 8-OHdG only in males as compared to their healthy controls. No correlation of the levels of any of two markers was seen with clinical severity of depression of patients as assessed by BDI. Conclusion: Evidence of oxidative stress to lipids and DNA are present in the peripheral blood. These can be explored further in establishing the biomarkers for diagnosis and prognosis of depression.

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