Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species- Endemic plant species of Western Ghats

Biomedicine ◽  
2021 ◽  
Vol 40 (4) ◽  
pp. 460-466
Author(s):  
Vinayak Lokapur ◽  
Varsha Jayakar ◽  
Manjula Shantaram
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Western Ghats ◽  
Skin Diseases ◽  
Antioxidant Properties ◽  
Physicochemical Analysis ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative ◽  
In Vitro Antioxidant Activity

Introduction and Aim: Plants from Anacardiaceae family are used in indigenous system of medicine for their anti-arthritic, antibacterial and antioxidant properties. Among them, Holigarna species are widely known as toxic members of Anacardeaceae family which are endemic to Western Ghats. It is commonly known as allergic plant which causes contact dermatitis if contacted. However, young leaves and seeds are used against haemorrhoids, obesity, and cancer and skin diseases. Despite the claims and the use of Holigarna ferrugenia, Holigarna nigra and Holigarna grahamii for the management of skin diseases in traditional medicine, little is known and documented so the aim of the study was to explore the in vitro antioxidant activity and to analyse qualitative and quantitative of phytochemicals along with their physicochemical properties. Materials and Methods: Physicochemical parameters such as moisture, pH, total ash, acid-insoluble ash, and elemental values were determined. Qualitative and quantitative analysis were carried out along with different antioxidant assays for evaluating the antioxidant activity of aqueous crude extract of H. ferrugenia, H. nigra and H. grahamii. Results: Physicochemical analysis revealed the presence of trace elements. Preliminary phytochemical analysis revealed the presence of tannins, alkaloids, flavonoids, phenols, saponins and steroids in aqueous crude extract of H. ferrugenia, H. nigra and H. grahamii. The free radical scavenging activity of all plants exhibited better antioxidant properties. Conclusion: The results indicated that the leaves contain an appreciable number of bioactive compounds and presence of these phytochemicals, especially the phenols and flavonoids could be the reason behind the use of both plants in ethno-medicine for the management of various ailments.

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

scholarly journals Phytochemical evaluation, HPTLC analysis and in-vitro antioxidant activity of hydroalcoholic leaf extract of Grewia hirsuta collected from Western Ghats forest

2020 ◽  
Vol 11 (3) ◽  
pp. 4653-4659
Author(s):  
Dattatraya Kature ◽  
Gaurav Gupta ◽  
Ritu Gilotra
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Western Ghats ◽  
High Performance ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity

In-vitro antioxidant action of hydroalcoholic leaf extract of Grewia hirsuta (HAEGH) has been examined using one, “1-diphenyl-2-picryl-hydrazil (DPPH) free from radical scavenging” actions. The plant collected from the forest of the Western Ghats region of Karnataka province. The motive for plant collection from a specific location is the plant of forests exhibits the variation in growth, quantity, and quality of their active ingredients and secondary metabolites due to influence ecological factors like effect changes in location, soil, climate, etc. The work corresponds to preliminary phytochemical investigation for diverse phytoconstituents and quantitative phytochemical analysis of total phenolic, flavonoids & alkaloids content (TPC, TFC & TAC respectively) was evaluated with advanced methods. “HPTLC (High performance thin-layer” chromatography) fingerprint investigation was achieved for qualitative determination of the likely number of elements present in the hydroalcoholic extract. In-vitro antioxidant activity of HAEGH has been determined through hydroxyl radical scavenging assay that exhibited strong dose-dependent antioxidant activity as compared with standards compound, ascorbic acid. The IC50 value of HAEGH found, 25.90 % inhibition and for ascorbic acid, it was 17.68%. The Preliminary phytochemical estimation found presence of flavonoids, alkaloids, glycosides, phenol, proteins, diterpins and quantitative phytochemical analysis estimation of TPC, TFC & TAC found to be 3.627%, 4.059% & 5.671% respectively. HPTLC analysis of HAEGH at 354nm reveals the presence of a compound with Rf value 0.44 compare with Rf value 0.46 of quercetin. These outcomes indicated that the hydroalcoholic leaf extract of Grewia hirsuta plant contains phytoconstituents that exhibit antioxidant activity possible because of the existence of bioactive compounds.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir

2012 ◽  
Vol 6 (1) ◽  
pp. 67 ◽  
Author(s):  
Mohd Mujeeb ◽  
Kamran Ashraf ◽  
Mohammad Aqil ◽  
Mohd Amir ◽  
Ahsanullah Khan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity

In vitro antioxidant activity and phytochemical analysis of Teucrium pseudo-Scorodonia Desf. Collected from Algeria

2017 ◽  
Vol 17 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Karima Belarbi ◽  
Fawzia Atik-Bekkara ◽  
Imad Abdelhamid El Haci ◽  
Ilhem Bensaid ◽  
Fawzia Beddou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity

scholarly journals Antioxidant Activity of Plant Extracts from Colombian Coffee-Growing Eco-Region

2017 ◽  
Vol 13 (1) ◽  
pp. 56-59
Author(s):  
Aura M. Blandón ◽  
Oscar M. Mosquera ◽  
Antônio E. G. Sant’ana ◽  
Aldenir F. Dos Santos ◽  
Luana L. S. Pires
Keyword(s):  
Antioxidant Activity ◽  
Plant Species ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Dpph Assay ◽  
Methanolic Extracts ◽  
In Vitro Antioxidant Activity

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of  Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND WOUND HEALING ACTIVITY OF WHEATGRASS BY 1,1- DIPHENYL, 2 PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (12) ◽  
pp. 170
Author(s):  
Veermaneni Alekhya ◽  
Thiyagarajan Deepan ◽  
Magharla Dasaratha Dhanaraju
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Wheat Plant ◽  
Wide Range ◽  
Wound Healing Activity ◽  
In Vitro Antioxidant Activity

Objective: This study was designed to evaluate in vitro antioxidant activity and wound healing activity in Triticum aestivum (wheat grass).Methods: T. aestivum commonly known as Wheatgrass had a wide range of health benefits among the young grass of common wheat plant components includes chlorophyll, flavonoids, and Vitamins A, C, and E. Wheatgrass is used in Folklore medicine for treatment of skin diseases and wound healing. In our present study, petroleum ether, ethanol and aqueous extracts of T. aestivum have been evaluated for in vitro antioxidant activity and wound healing activity by 1,1- diphenyl, 2 Picrylhydrazyl radical scavenging activity, and Chick chorioallantoic method, respectively.Results: The results of both the assay showed that all the extracts of T. aestivum have significant antioxidant and wound healing activity on dose-dependent manner.Conclusion: The wheatgrass has antioxidant and wound healing activity.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY, IN VIVO ANTIPYRETIC AND ANTI-INFLAMMATORY ACTIVITIES OF PARONYCHIA CAPITATA L.

2017 ◽  
Vol 10 (3) ◽  
pp. 177
Author(s):  
Soumia Bouzidi ◽  
Benkiki Naima ◽  
Allaoua Zina
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Butanol Extract ◽  
Antipyretic Activity ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

ABSTRACTObjective: The aim of this study is to evaluate the in vitro antioxidant activity of Paronychia capitata various extracts, acute toxicity, the in vivoantipyretic and anti-inflammatory activities of the n-butanol extract.Methods: The antioxidant properties were tested using 2,2-diphenyl 1-picrylhydrazyl radical scavenging and β-carotene linoleic acid system, thetotal phenolic and flavonoid contents were determined. Furthermore, anti-inflammatory activity and antipyretic activity of the n-butanol extractwere assessed on male Wistar rats at the dose levels 250, 500 mg/kg body weight, using the egg albumin-induced edema, and brewer’s yeast-inducedpyrexia method, respectively.Results: The n-butanol extract of P. capitata had considerably the highest antiradical activity with inhibitory concentration 50 value of 8.667 μg/ml.Conclusion: The experimental data demonstrated that n-butanol extract of P. capitata possess significantly remarkable anti-inflammatory andantipyretic activities compared to the standard drugs.Keywords: Caryophyllaceae, Flavonoid, Inflammation, Fever, Paronychia capitata.

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