scholarly journals Dose dependent effect of silver nitrate on enhanced production of gymnemic acid in cell suspension cultures of Gymnemasylvestre R. Br

Biomedicine ◽  
2021 ◽  
Vol 41 (1) ◽  
pp. 16-22
Author(s):  
Jarubandi Rajashekar ◽  
Vadlapudi Kumar ◽  
DittekoppaVijendra Poornima ◽  
Gajula Hari ◽  
Torankumar Sannabommaji ◽  
...  
Keyword(s):  
Silver Nitrate ◽  
Cell Suspension ◽  
Acid Production ◽  
Triterpenoid Saponins ◽  
Dependent Effect ◽  
Gymnemic Acid ◽  
Enhanced Production ◽  
Mda Content ◽  
Dose Dependent ◽  
Dose Dependent Effect

  Introduction and Aim: Gymnemasylvestre is rich source of triterpenoid saponins and is popularly known for its anti-diabetic potential. Gymnemic acid is chiefly responsible for its therapeutic potential. In fact, gymnemic acid is a group of compounds known as gymnemagenin (oleanane group) and other related compounds are gymnemasides (dammaranes). Despite of its immense medicinal importance, very few studies were conducted for enhanced gymnemic acid production. The present study undertaken for the enhanced gymnemic acid production using silver nitrate (AgNO3) as effective elicitor and its influence on other culture parameters.   Materials and Methods:Callus culture was initiated for cell suspension culture establishment and is used to study the elicitor effect. Different doses of AgNO3 used at varied culture periods and its mediated effect on growth index, pH, EC and MDA content was conducted. Gymnemic acid production was estimated by HPLC.   Results:Cell growth was positively influenced by AgNO3 with concomitant change in pH and EC of the medium. Apparent rise in MDA content in response to increased AgNO3 dosage was observed and is positively correlated with enhanced gymnemic acid production. Dose dependent effect of AgNO3 was observed at different culture periods used under study.  Conclusion: Substantial improvements of gymnemic acid produced in response to AgNO3 at 100µM dosage can be used for its enhanced production at industrial scale. It also offers opportunities for studying elicitor influence on other parameters for gymnemic acid production

scholarly journals Silver‐doped sol‐gel borate glasses: Dose‐dependent effect on Pseudomonas aeruginosa biofilms and keratinocyte function

2021 ◽  
Author(s):  
Shiva Naseri ◽  
Gabriele Griffanti ◽  
William C. Lepry ◽  
Vimal B. Maisuria ◽  
Nathalie Tufenkji ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sol Gel ◽  
Borate Glasses ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect

Dose-Dependent Effect of Statins on the Incidence of Acute Kidney Injury After Cardiac Surgery

2011 ◽  
Vol 91 (2) ◽  
pp. 520-525 ◽  
Author(s):  
Salima Mithani ◽  
Michael Kuskowski ◽  
Yelena Slinin ◽  
Areef Ishani ◽  
Edward McFalls ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Cardiac Surgery ◽  
Kidney Injury ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect

Ligand-Regulated Expression of TNF Receptors 1 and 2 Determines Receptor-Mediated Functional Responses

2021 ◽  
pp. 1-12
Author(s):  
Alina Alshevskaya ◽  
Olga Koneva ◽  
Irina Belomestnova ◽  
Julia Lopatnikova ◽  
Irina Evsegneeva ◽  
...  
Keyword(s):  
Biological Effects ◽  
High Expression ◽  
Functional Responses ◽  
Double Positive ◽  
Dependent Effect ◽  
Similar Expression Profile ◽  
Proliferation Response ◽  
Dose Dependent ◽  
Dose Dependent Effect ◽  
Mcf 7

<b><i>Introduction:</i></b> Modulating specific biological effects through the changes in cytokine receptors’ expression level remains poorly understood. This study aimed to investigate the influence of the dose-dependent effect of TNF on the balance between proapoptotic and proliferation response depending on the parameters of TNFR1/2 expression density. <b><i>Methods:</i></b> Tumor cell lines (HEp-2, K-562, MCF-7, ZR-75/1, MOLT-4, IM-9, and Raji) were characterized for TNFR1/2 co-expression using flow cytometry and were studied to reveal the dose-dependent effect of rhTNF on cell cycle and apoptosis parameters. The associations among the studied parameters were estimated by correlation and regression analysis. <b><i>Results:</i></b> It was found for ZR-75/1 cells (the cell line characterized by high expression of both types) that a dose-dependent increase in expression of both types of TNF-α receptors on cells reduces the proliferative activity of cells. For MOLT-4 cells (which are characterized by lower expression), an increase in proliferative response of cells was positively associated with the percentage of both TNFR1<sup>+</sup> and TNFR2<sup>+</sup> cells. However, opposite effects on the cells were shown for the K-562 and MCF-7 lines having a similar expression profile. A similarity (a large percentage of double-positive cells) was revealed for the lines having similar effects (K-562 and ZR-75/1). <b><i>Conclusions:</i></b> High expression of TNF receptor type 1 is not always associated with predominant activation of proapoptotic pathways. However, in the case of simultaneous high expression of both types of receptors, the proportion of double-positive cells is crucial for the activation of either the proapoptotic or proliferation pathways.

scholarly journals Dose-dependent effect of low-intensity pulsed ultrasound on callus formation during rapid distraction osteogenesis

2006 ◽  
Vol 24 (11) ◽  
pp. 2072-2079 ◽  
Author(s):  
Chun Wai Chan ◽  
Ling Qin ◽  
Kwong Man Lee ◽  
Wing Hoi Cheung ◽  
Jack Chun Yiu Cheng ◽  
...  
Keyword(s):  
Distraction Osteogenesis ◽  
Callus Formation ◽  
Pulsed Ultrasound ◽  
Dependent Effect ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Dose Dependent ◽  
Dose Dependent Effect

Dose-Dependent Effect of Octreotide on Insulin Secretion after OGTT in Obesity

1997 ◽  
Vol 49 (1) ◽  
pp. 17-21 ◽  
Author(s):  
A. Bertoli ◽  
R. Magnaterra ◽  
P. Borboni ◽  
M.A. Marini ◽  
A. Barini ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect

scholarly journals Evaluation of cyclooxygenase-2 inhibition in an orthotopic murine model of lung cancer for dose-dependent effect

2005 ◽  
Vol 129 (6) ◽  
pp. 1242-1249 ◽  
Author(s):  
Eric M. Sievers ◽  
Robert D. Bart ◽  
Leah M. Backhus ◽  
Yuanguang Lin ◽  
Margaret Starnes ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Murine Model ◽  
Cyclooxygenase 2 ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect
Sign in / Sign up

Export Citation Format

Share Document