scholarly journals HIGH-RESOLUTION CT (HRCT) CHEST FINDINGS IN PCR POSITIVE PATIENTS OF COVID19 AT COMBINED MILITARY HOSPITAL RAWALPINDI

2021 ◽  
Vol 71 (3) ◽  
pp. 951-54
Author(s):  
Javed Ahmad Khan ◽  
Faryal Asmat ◽  
Fuad Ahmad Siddiqi ◽  
Tariq Bashir Tareen ◽  
Fayyaz Hassan ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
High Resolution ◽  
British Society ◽  
Ground Glass ◽  
Mediastinal Lymphadenopathy ◽  
Military Hospital ◽  
Chain Reaction ◽  
High Resolution Computed Tomography ◽  
Polymerase Chain

Objective: To study High resolution computed tomography chest findings in polymerase chain reaction (PCR) positive patients of COVID-19. Study Design: Cross sectional study. Place and Duration of Study: Combined Military Hospital Rawalpindi, from May to Jun 2020. Methodology: Study included high resolution computed tomography chest of 200 patients admitted to COVID ward who were polymerase chain reaction positive. Patients having previously diagnosed Interstitial lung disease were excluded. Informed consent from patients were taken. Different findings on High resolution computed tomography chest were summarized as percent of total cases. British society of thoracic imaging (BSTI) classification was used to classify high resolution computed tomography chest patterns of COVID-19 pneumonia. Results: Out of 200 patients included in study, 7 had previous changes on their chest imaging and were excluded. 193 patients (145 males and 48 females) enrolled had a median age of 52 years. One hundred and two patients (52.9%) showed classical pattern for COVID-19, 81 patients (41.9%) had indeterminate changes, 3 patients (1.6%) had atypical changes while 7 (3.6%) had normal high resolution computed tomography chest. Four had pleural effusions while 5 had mediastinal lymphadenopathy. One hundred and eight nine (97.9%) patients had bilateral lung involvement, 145 (75.1%) had central and peripheral while 48 (24.9%) had peripheral invol-vement only. One hundred and forteen (59%) patients had ground glass opacities while 70 (36.3%) had ground glass opacities with consolidation. Nine (4.7%) patients presented with consolidation only. Conclusion: High resolution computed tomography chest and COVID polymerase chain reaction done at the same time.........

scholarly journals Clinical Characteristics of Patients With Coronavirus Disease 2019 in Japan: A Single-Center Case Series

2020 ◽  
Vol 222 (2) ◽  
pp. 194-197 ◽  
Author(s):  
Ken-ichiro Kobayashi ◽  
Takahiro Kaki ◽  
Shinsuke Mizuno ◽  
Kenji Kubo ◽  
Nobuhiro Komiya ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Clinical Characteristics ◽  
Case Series ◽  
Ground Glass ◽  
Single Center ◽  
Chain Reaction ◽  
Pharyngeal Swab ◽  
Polymerase Chain

Abstract We report a case series of 6 patients with confirmed coronavirus disease 2019 (COVID-19) in Wakayama prefecture, Japan. All 6 of the patients tested positive via pharyngeal swab polymerase chain reaction (PCR) tests, and 2 of the 6 were still positive at 3 weeks after onset. All of the patients exhibited bilateral ground glass opacities on computed tomography (CT). This article also reports narrative information on the spectrum of symptoms collected directly from the patients. It would be difficult to triage patients with COVID-19 based on the typical symptoms of fever and/or cough, although PCR and CT are definitive in diagnosis.

scholarly journals A Case Series of Patients Suspected to Have Covid-19 Based on Clinical Features, Laboratory Markers and Imaging Studies but Who Were RT-PCR Negative

2021 ◽  
Vol 8 (15) ◽  
pp. 982-988
Author(s):  
Dilip Pandurang Patil ◽  
Nikhil Dilip Patil
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Clinical Features ◽  
Case Series ◽  
Rt Pcr ◽  
Imaging Studies ◽  
Chain Reaction ◽  
High Resolution Computed Tomography ◽  
Laboratory Markers ◽  
Polymerase Chain

BACKGROUND Real-time reverse transcription polymerase chain reaction (rRTPCR) test is the gold standard assay to diagnose Covid-19 in-spite of few concerns. The disease is suspected in patients presenting with clinical features suggestive of Covid-19 supported by laboratory markers and imaging studies. We describe a case series of ten patients suspected to have SARS CoV-2 infection but RTPCR negative. METHODS We carried out a retrospective observational study of patients who presented with clinical features suggestive of Covid-19 but were RTPCR negative. As dedicated Covid hospitals, RTPCR negative patients were not admitted because of overload. We admitted such patients in isolation ward in non Covid hospitals, evaluated them further with laboratory biomarkers, and imaging studies. All patients were categorised in disease severity and were managed according to guidelines of Ministry of Health and Family Welfare (MoHFW) India. RESULTS Our case series included ten patients with a mean age of 55.1 years and the male to female ratio was 1.5:1. All patients (100 %) were suspected to have SARS CoV2 infection. Commonest symptom was fever in all (100 %) and least common was diarrhoea in 30 % patients. Mean SpO2 was 92.5 % on pulse oximeter (range 91 to 94 %). The laboratory findings showed (median): white blood cell count 9400 / cumm, N / L ratio 3.78, C-reactive protein (CRP) 46.21 mg / L, D-dimer 465 ng / mL, lactate dehydrogenase 499.59 U / L, serum ferritin 160.55 ng / mL, interleukin 6 31.6 pg / mL. Chest radiograph showed patchy non homogenous opacities in lungs in 6 (60 %) out of 10 patients, high resolution computed tomography (HRCT) chest revealed peripheral ground glass opacities (GGOs) in 10 (100 %) patients, Coronavirus Disease 2019 Reporting and Data System (CORADS) score was 5 in 8 (80 %) patients and less than 5 in 2 (20 %) patients. CONCLUSIONS In current pandemic, patients presenting with clinical features suggestive of Covid19, but RT-PCR negative should be suspected to have SARS CoV-2 infection, further evaluation with laboratory markers and imaging study help in diagnosis. Managing and monitoring according to MoHFW guidelines show good clinical recovery. KEYWORDS Corona Virus Disease (Covid-19), Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR), High Resolution Computed Tomography (HRCT), Chest X-Ray (CXR)

scholarly journals Contribution of neutrophil/lymphocyte ratio to the diagnostic efficiency of computed tomography and polymerase chain reaction in COVID-19 patients

2021 ◽  
Vol 9 ◽  
pp. 205031212110464
Author(s):  
Serhat Örün ◽  
Mustafa Numan Erdem
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Ground Glass ◽  
Care Hospital ◽  
Chain Reaction ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Computed Tomography Images ◽  
The Mean ◽  
Polymerase Chain

Background: 6.5% of the country’s population was diagnosed with COVID-19 disease. Computed tomography scanning and polymerase chain reaction tests are considered reliable methods for the detection of COVID-19. However, the specificity and reliability of polymerase chain reaction tests and ground-glass opacity (GGO) on thorax computed tomography images in diagnosing COVID-19 are still being disputed. Our aim was to compare the neutrophil/lymphocyte ratio, whose efficiency in differentiating between viral and bacterial infections has previously been studied, with computed tomography and polymerase chain reaction for COVID-19 diagnosis. Materials and methods: This was a retrospective study that included patients treated in a tertiary care hospital emergency service pandemic polyclinic between 14 March and 1 June 2020. The neutrophil/lymphocyte ratios of patients with polymerase chain reaction tests and ground-glass opacities on computed tomography were calculated. The neutrophil/lymphocyte ratios of polymerase chain reaction-negative patients with computed tomography images were compared with the neutrophil/lymphocyte ratios of polymerase chain reaction-positive patients with computed tomography images. Results: A total of 631 patients were included in this study. Thorax computed tomography scans were obtained from all patients. The mean neutrophil/lymphocyte ratio of patients with ground-glass opacities was 3.50 ± 2.12, whereas that of patients without ground-glass opacities was 2.90 ± 2.01. This difference was also statistically significant. Polymerase chain reaction swab samples were obtained from 282 patients (44.7%). The mean neutrophil/lymphocyte ratio of polymerase chain reaction-positive patients was 2.38 ± 1.02, whereas that of polymerase chain reaction-negative patients was 3.97 ± 2.25. The difference was statistically significant. Conclusion: Many studies are undoubtedly required to determine the efficiency of the neutrophil/lymphocyte ratio in COVID-19 diagnosis. However, we postulate that evaluating the neutrophil/lymphocyte ratio along with computed tomography and polymerase chain reaction can assist in the diagnosis of patients.

scholarly journals Chest X-Ray in Coronavirus Disease 2019 (COVID-19) Infection: Findings and Correlation with Clinical Outcome at Level-3 Nepalgunj Medical College and Teaching Hospital Kohalpur

2020 ◽  
Vol 18 (1) ◽  
pp. 74-77
Author(s):  
Shyam Kumar BK ◽  
Sumit Pandey ◽  
Nabin Poudel ◽  
Sandesh Pandit ◽  
Alok Kumar Sah ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Virus Disease ◽  
Ground Glass ◽  
X Rays ◽  
Chain Reaction ◽  
Medical College ◽  
Corona Virus ◽  
Chest X Ray ◽  
Polymerase Chain

Introduction: At the end of 2019 a novel virus, named SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), expanded globally from China. A new coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), was identified as the cause of this outbreak of viral pneumonia that causes coronavirus disease 2019 (COVID-19). Aims: The aim of this study is to find out the chest radiological features of corona virus disease patients and correlate them with clinical outcome. Methods: This is a Hospital based study involving patients with clinical-epidemiological aspect of all reverse transcription polymerase chain reaction (RT-PCR) corona virus disease (COVID-19) positive patients, who performed Chest X-Rays at the emergency department of Nepalgunj Medical College, Teaching Hospital from March to June, 2020. All patients performed reverse transcription polymerase chain reaction from nasopharyngeal and throat swab, Chest X-Ray at the Emergency Department and clinical-epidemiological data. Results: Patients with a reverse transcription polymerase chain reaction positive results for corona virus disease infection were 32 out of these, 22 were females (68.75%) and 10 males (31.25%), with a mean age of 40.78 years (range 20–74 years). Only 2 Chest X-Rays were negative for radiological thoracic involvement (6.25%).  The following alterations were more commonly observed among 30 patients: 18 patients with lung consolidations (56.25%), 19 (59.37%) with Ground Glass Opacities, 7 (21.87%) with nodules and 21 (65.6%) with reticular–nodular opacities. Patients with consolidations and Ground Glass Opacities coexisting in the same radiography were 34.37% of total. In reverse transcription polymerase chain reaction positive patients, we found also signs nonspecific for corona virus disease pneumonia as hilar or vascular congestion (37.5%), cardiomegaly (28.12%), pleural effusion (15.6%) and pneumothorax (3.12%). Peripheral (56.25%) and lower zone distribution (56.25%) were the most common predominance. Bilateral involvement (68.75%) was most frequent than unilateral one. Given the results, baseline Chest X-Rays sensitivity in our experience is about 65.62%. Conclusion: In this study, COVID-19 CXRs generally manifested a spectrum of pure ground glass, mixed ground glass opacities to consolidation in bilateral peripheral middle and lower lung zones. BSTI CXR reporting classification of COVID-19 is valid and sensitive in our patients with addition of middle zonal involvement in classical COVID-19 criteria as opposed to just lower zone involvement.

scholarly journals High-Resolution Melting-Based Quantitative Analysis of RASSF1 Methylation in Breast Cancer

Medicina ◽  
2013 ◽  
Vol 49 (2) ◽  
pp. 14 ◽  
Author(s):  
Kristina Stuopelytė ◽  
Kristina Daniūnaitė ◽  
Aida Laurinavičienė ◽  
Valerijus Ostapenko ◽  
Sonata Jarmalaitė
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
High Resolution ◽  
Quantitative Methods ◽  
High Resolution Melting ◽  
Cost Effective ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Breast Tissues

Background and Objective. Breast cancer is the leading cause of death from cancer among women worldwide. The aberrant promoter methylation of tumor suppressor genes is a typical epigenetic alteration for breast cancer and can be detected in early carcinogenesis. High-throughput and cost-effective methods are needed for the early and sensitive detection of epigenetic changes in clinical material. The main purpose of our study was to optimize a high-resolution melting (HRM) assay for the reliable and quantitative assessment of RASSF1 gene methylation, which is considered one of the earliest epigenetic alterations in breast cancer. Material and Methods. A total of 76 breast carcinomas and 10 noncancerous breast tissues were studied by means of HRM and compared with the results obtained by means of quantitative methylation-specific polymerase chain reaction (QMSP) and methylation-specific polymerase chain reaction (MSP). Results. Both quantitative methods, HRM and QMSP, showed a similar specificity and sensitivity for the detection of RASSF1 methylation in breast cancer (about 80% and 70%, respectively). In breast cancer, the mean methylation intensity of RASSF1 was 42.5% and 48.6% according to HRM and QMSP, respectively. Both methods detected low levels of methylation (less than 5%) in noncancerous breast tissues. In comparison with quantitative methods, MSP showed a lower sensitivity (70%), but a higher specificity (80%) for the detection of RASSF1 methylation in breast cancer. Conclusions. HRM is as a simple, cost-effective method for the reliable high-throughput quantification of DNA methylation in clinical material.

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