scholarly journals THE STABILITY OF THE PACKED CELL VOLUME OF ANTICOAGULATED BOVINE BLOOD STORED AT REFRIGERATION TEMPERATURE.

2021 ◽  
Vol 18 ◽  
pp. 111-112
Author(s):  
I. O. IGBOKWE ◽  
N. M. SANU
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Ethylene Diamine ◽  
Bovine Blood ◽  
Blood Samples ◽  
Plasma Fractions ◽  
Ethylene Diamine Tetra Acetate ◽  
The Stability

Bovine blood samples with ethylene diamine tetra-acetate (EDTA) or oxalate as anticoagulant were stored at refrigeration temperature of 4°C. The packed cell volume (PCV) was determined daily. During storage, the PCV did not vary significantly. Complete haemolysis, suggested by failure of blood samples to separate into cellular and plasma fractions, started occuring in samples on days 28 and 17, and was observed to have occurred in all samples on days 41 and 24 in blood with EDTA and oxalate respectively.

scholarly journals Some physiological and chemical properties of horse and cow blood samples under effect of some different types of anticoagulants

2018 ◽  
Vol 42 (1) ◽  
pp. 7-11
Author(s):  
Muna M. Ismail
Keyword(s):  
Acetic Acid ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Chemical Properties ◽  
Acid Group ◽  
Ion Concentration ◽  
Ethylene Diamine ◽  
Sodium Oxalate ◽  
Heparin Group ◽  
Blood Samples

     The purpose of the present study is to determine and compare the anticoagulants, Ethylene diamine tetra acetic acid, Sodium oxalate, Sodium citrate and heparin in blood samples of horse and bull, through estimation of (Hemoglobin, Packed Cell Volume and Plasma Platelets Count) and  plasma physical properties (clarity and volume) and some chemical properties (pH and calcium ion concentration). Five Blood samples were obtained from five stallions and five bulls (20 ml/sample) and were divided as 5 ml in four test tubes containing Ethylene diamine tetra acetic acid, sodium oxalate, sodium citrate and heparin at the College of Veterinary Medicine, University of Diyala. Hemoglobin and packed cell volume were estimated immediately and then the blood samples were centrifuged for 15 min. at 3000 rpm to obtain plasma for evaluating the physical and chemical properties specified above. The horse blood samples pertaining data had no significant elevation in hemoglobin concentration and packed cell volume in Ethylene diamine tetra acetic acid group as compared to other groups, beside having no significant changes in pH between the four treated groups while there was a significant increase in plasma volume of oxalate and citrate as compared to Ethylene diamine tetra acetic acid and heparin, and there was a significant increase in plasma platelet count of Ethylene diamine tetra acetic acid group as compared to heparin. As well there was a significant decrease of calcium ion concentration in Ethylene diamine tetra acetic acid, oxalate and citrate as compared to heparin, while the results of bull blood samples revealed that the hemoglobin and Packed Cell Volume were significantly higher in heparin group than in the oxalate and citrate groups with non significant differences with Ethylene diamine tetra acetic acid group. The plasma obtained from different types of anticoagulants appeared with a high degree of clarity, and the volume of plasma had no significant increase in oxalate and citrate group as compared to other groups. The Plasma Platelet Count and calcium concentration significantly increased in heparin group as compared to other groups. In conclusion, the Ethylene diamine tetra acetic acid was more reliable for horse blood samples while the anticoagulant heparin was more reliable anticoagulant for bull blood samples.

The blood composition of Friesian bulls; variations with age and relationships with results of progeny tests

1978 ◽  
Vol 91 (3) ◽  
pp. 579-585 ◽  
Author(s):  
A. J. Stark ◽  
G. J. Rowlands ◽  
R. Manston ◽  
A. E. McClintock
Keyword(s):  
Dairy Cows ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Inorganic Phosphate ◽  
Mean Corpuscular Volume ◽  
Cattle Breeding ◽  
Blood Samples ◽  
Progeny Tests ◽  
Milk Marketing ◽  
Cell Volumes

SummaryBlood samples were taken from 172 Friesian bulls during 1973–5 on three or four occasions. The bulls were in Milk Marketing Board cattle breeding centres in England and Wales and were from 1 to 14 years of age.The blood samples were analysed for packed cell volume, haemoglobin, erythrocytes, mean corpuscular volume and glucose, and samples of serum for the concentrations of albumin, total protein, urea-nitrogen, inorganic phosphate, Ca, Mg, K, Na and Cu.Packed cell volumes and haemoglobin and K concentrations were higher in bulls than those previously recorded in dairy cows. There were significant age relationships for packed cell volume, haemoglobin, albumin and globulin (P < 0·001), inorganic phosphate and erythrocytes (P < 0·01) and Ca and Mg (P < 0·05).Repeatability estimates were calculated and compared with estimates previously obtained for dairy cows.On the basis of improved contemporary comparisons of the bulls, there was a significant relationship between the average milk yield of a bulls daughters and the concentrations of urea, inorganic phosphate and K (P < 0·05) in the blood serum of the bull.

The effect of season on the composition of the blood of lactating and non-lactating cows as revealed from repeated metabolic profile tests on 24 dairy herds

1974 ◽  
Vol 83 (1) ◽  
pp. 27-35 ◽  
Author(s):  
G. J. Rowlands ◽  
W. Little ◽  
R. Manston ◽  
Sally M. Dew
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Dairy Herds ◽  
Urea Nitrogen ◽  
Blood Samples ◽  
Mean Values ◽  
Lactating Cows ◽  
Nitrogen Concentrations ◽  
Volume Blood ◽  
Autumn And Winter

SUMMARYBlood samples were taken from groups of lactating and non-lactating cows on three occasions from each of 24 commercial dairy herds sampled eight herds at a time at 6-weekly intervals throughout 1972. The blood samples were analysed for packed cell volume, blood glucose and haemoglobin, and for the serum concentrations of albumin, total protein, urea nitrogen, Ca, inorganic phosphate, Mg, K, Na, Cu, and Fe.Clear seasonal patterns were evident for packed cell volume and the mean concentrations of urea nitrogen and haemoglobin each of which were highest during the summer months. The effect of season on the concentrations of the other constituents was smaller. Mean values of packed cell volume, haemoglobin and Fe were consistently higher in the non-lactating cows than in the lactating cows. Mean concentrations of Mg were lower in the blood of non-lactating cows throughout the year; Cu concentrations followed a similar trend in the autumn and winter.There was evidence of overall herd differences regardless of season, in particular for Cu, globulin, Fe and urea nitrogen concentrations.

The effect of plane of nutrition on the response by Trypanotolerant cattle to infection with Trypanosomiasis

1994 ◽  
Vol 1994 ◽  
pp. 148-148
Author(s):  
D L Romney ◽  
A Njie ◽  
P Holmes ◽  
M Gill
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Samples ◽  
Groundnut Cake ◽  
Domestic Livestock ◽  
Negative Effect ◽  
Ad Libitum ◽  
Trypanotolerant Cattle

Tse-Tse flies inhabit over 11 million km2 of Africa and are vectors of trypanosomes which cause trypanosomiasis in domestic livestock. The disease has a negative effect on productivity and is fatal in non tolerant breeds. The effect of nutrition on response to the disease was studied.Thirty two N'Dama heifers (initial mean liveweight 113 + 17.0) were offered ad libitum andropogon hay plus 10.2 g/kg LW groundnut hay (GNH) (L) or GNH and 3.9 g/kg LW groundnut cake (GNC) (H). After 4 weeks on diet, half of each group were inoculated intradermally with trypanosomes (T. Congolense) (LI and HI). Intake was measured daily and liveweights weekly, while thrice weekly blood samples were examined for parasitaemia and packed cell volume (PCV).

Spontaneous drinking: is it stimulated by hypertonicity or hypovolemia?

1990 ◽  
Vol 258 (1) ◽  
pp. R143-R148
Author(s):  
T. R. Houpt ◽  
C. R. Anderson
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Protein ◽  
Packed Cell Volume ◽  
Young Female ◽  
Protein G ◽  
Base Line ◽  
Blood Samples ◽  
Spontaneous Drinking

Ten young female pigs were allowed to eat and drink whenever they wanted, and blood samples were taken without disturbance to the pig. Samples were divided into four categories: 1) base line, i.e., taken when not drinking and not eating; 2) preprandial, i.e., taken when starting to drink just before eating; 3) postcibal, i.e., taken when starting to drink after any eating; and 4) nonprandial, i.e., taken when starting to drink but not in association with eating. Osmolality (mosmol/kgH2O), plasma protein (g/dl), and packed cell volume (%) were, respectively, as follows (mean +/- SE): base line 294.9 +/- 1.8, 6.2 +/- 0.1, and 30.0 +/- 1.2; preprandial 295.1 +/- 1.7, 6.1 +/- 0.2, and 29.7 +/- 1.4; postcibal 295.0 +/- 3.3, 6.5 +/- 0.2, and 31.7 +/- 1.3; and nonprandial 295.2 +/- 1.4, 6.3 +/- 0.1, and 30.0 +/- 1.3. None of the parameters associated with drinking were significantly different from base-line parameters, except for the postcibal rise of packed cell volume. Calculated blood volume differences from base line were as follows: preprandial 0.7 +/- 1.2% fall; postcibal 0.2 +/- 1.7% increase; and nonprandial 0.8 +/- 0.9% fall. It is unlikely that either plasma hypertonicity or hypovolemia stimulates drinking under spontaneous conditions.

Glucose determinations in plasma and serum: potential error related to increased hematocrit.

1982 ◽  
Vol 28 (1) ◽  
pp. 190-192 ◽  
Author(s):  
R A Sidebottom ◽  
P R Williams ◽  
K S Kanarek
Keyword(s):  
Blood Glucose ◽  
Cord Blood ◽  
Cell Volume ◽  
Plasma Glucose ◽  
Packed Cell Volume ◽  
Blood Samples ◽  
Potential Error ◽  
Blood Specimens

Abstract This study reports the effect of increased hematocrit (packed cell volume) on the rate of decrease in plasma glucose during 6 h in blood specimens from seven newborns (cord blood) and heparin-treated blood specimens from eight adults. Mean hematocrits ranged frm 0.43 to 0.75 for the adults and from 0.51 to 0.81 for the infants. The decrease in glucose was related to time and to hematocrit. For example, in adults with a mean hematocrit of 0.43, glucose decreased from 890 +/- 40 mg/L to 570 +/- 30 mg/L (mean +/- SEM) at 6 h, whereas in adults with a mean hematocrit of 0.75, glucose decreased from 890 +/- 40 to 200 +/- 30 mg/L. In infants with a mean hematocrit of 0.51, glucose decreased from 1010 +/- 20 to 480 +/- 70 mg/L at 6 h; infants with a mean hematocrit of 0.81 had blood glucose decrease from 1010 +/- 20 to 50 +/- 30 mg/L. These results indicate that the incidence of hypoglycemia in infants with polycythemia may be overestimated, and emphasize the need for prompt handling of blood samples collected from newborns for glucose determinations.

scholarly journals Effects of storage duration on haematological parameters of the red eared slider - Trachemys Scripta Elegans

2020 ◽  
Vol 51 (6) ◽  
pp. 639-644
Author(s):  
Nejra Hadžimusić ◽  
Dunja Rukavina ◽  
Vedad Škapur ◽  
Lejla Velić
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Healthy Adult ◽  
Haematological Parameters ◽  
Blood Cell Count ◽  
Storage Duration ◽  
Blood Samples

Reptiles, especially turtles, are becoming increasingly popular as pets. The haematological evaluation of turtles is an irreplaceable diagnostic tool in veterinary practice. However, the morphologic distinctiveness of turtle blood limits the use of electronic cell-counting devices, making time-consuming, manual counting techniques and evaluation of blood smears necessary. Many samples are dispatched to a laboratory over long distances, where a delay of 24 h or more may occur. At weekends, this interval may exceed 48 h. The objective of the present study was to determine the effect of storage duration at refrigerator temperature (4 °C) on the counts of red blood cells (RBC) and white blood cells (WBC), and on the mean corpuscular volume (MCV) and packed cell volume (PCV) in blood samples from healthy adult red-eared sliders. Blood samples were collected by venipuncture from the occipital venous sinus from six apparently healthy adult red-eared sliders, aged 2 to 4 years. Blood samples were analysed immediately after sampling to obtain the baseline value (BV) of the red blood cell count, white blood cell count and packed cell volume percentage. Blood was stored at 4 °C and the haematological analyses were performed after 24h, 48h and 72h. The results showed the same level of stability for RBC and WBC count, and MCV values during 72 hours of storage at 4 °C and for PCV during 48 hours. Handling of blood samples, and duration of storage of the blood samples can significantly influence the results/values of haematological tests. Consequently, the obtained values of the determined haematological parameters of improperly stored or handled blood samples can give a misleading interpretation of the results on the animal’s health status.

Sign in / Sign up

Export Citation Format

Share Document