THE STABILITY OF THE PACKED CELL VOLUME OF ANTICOAGULATED BOVINE BLOOD STORED AT REFRIGERATION TEMPERATURE.

Bovine blood samples with ethylene diamine tetra-acetate (EDTA) or oxalate as anticoagulant were stored at refrigeration temperature of 4°C. The packed cell volume (PCV) was determined daily. During storage, the PCV did not vary significantly. Complete haemolysis, suggested by failure of blood samples to separate into cellular and plasma fractions, started occuring in samples on days 28 and 17, and was observed to have occurred in all samples on days 41 and 24 in blood with EDTA and oxalate respectively.

Analytical measures (tests) recommended before delivery in a labour room for female

The study was conducted at central hospital Saidu Sharif, Swat, Khyber Pakhtunkhwa from April to September 2020. A total of 100 pregnant women belonging to different regions of district Swat were examined, prior to delivery in the research study. The age of the studied participants was 20-45 years. 3ml of venous blood was taken in a sterile syringe (3cc) from each woman and drained in EDTA (Ethylene diamine tetra acetate) tube. The tube was immediately carried to Sheikh-Al-Zahid-bin-Haiyan (casualty) hospital’s laboratory for complete blood count (CBC), Blood sugar (RBS). Human immunodeficiency virus (HIV), HBS-Ag, Hepatitis C virus (HCV), malarial parasites (MP), Brucella and widal test. Urine was also collected in urine bottles for examination and for COVID-19 detection swab samples were taken from nose of the participants. From the recent study it was concluded that pregnant women in the study are susceptible to many diseases and complications. It was found that 12% women were severe anemic while 23% were moderate anemic and 40% were mild anemic. Covid-19 was detected in 12% of the studied women. 2% were observed as HCV positive. 10% of the women had higher blood sugar level (RBS 190mg/dl). 35% of the studied women had a urinary tract infection (UTI). Only 1% of the participant was found to be affected by Brucella bacteria. Malarial parasite was found in almost 5% of the examined participants. HIV was rare and detected in only 1% of the examined one hundred women.

Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk K2EDTA dan K3EDTA. K3EDTA biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

Ekstrak Daun Asam Jawa (Tamarindus Indica L.) Sebagai Antidiabetes

One of the plant that is widely used as a medicinal plant is the leaves of tamarind (tamarindus indica L). This study aims to determine the effectiveness of anti-diabetic of tamarind (tamarindus indica L.) extract in lowering blood glucose levels in mice animals test. The experiment was performed using tamarind leaves extracted through infusion method. The animals test were 15 male mice induced by ethylene diamine tetra acetate (EDTA). Mice were divided randomly into 5 groups with different treatments. Treatments I, II and III were given tamarind leaves extracts for each with a concentration of 10%, 20% and 40%. Treatment IV was given glibenclamide suspense as a positive control (+), and the treatment V was given Na-CMC 1% as a negative control (-). Based on the analysis of variance statistical calculations tamarind leaves extracts can lower blood glucose levels on mice test. Furthermore, the significant difference was tested to check the concentration effect. The results on animals test with a significant level of 5% showed that the tamarind leaves extract of 40% or Treatment III affected on decreasing blood sugar levels on mice.